The amplified fragment length polymorphic(AFLP)technique was used to analyze the genome DNA polymorphism among 8 breeds of domestic ducks and 2 species of wild ducks.Nine of the 17 selected primers pairs gave reproduc...The amplified fragment length polymorphic(AFLP)technique was used to analyze the genome DNA polymorphism among 8 breeds of domestic ducks and 2 species of wild ducks.Nine of the 17 selected primers pairs gave reproducible polymorphic DNA amplification bands.The amplified bands ranged from 44 to 83 per primer pair.Of the 513 AFLP markers obtained,498 were polymorphic.The proportion of polymorphic loci was 97.1%.The genetic distance(D)and similarity coefficients(GS)were calcu-lated based on the polymorphic data.Between domestic ducks D ranged from 0.331 to 0.589,while between domes-tic ducks and the wild ducks,it ranged from 0.298 to 0.520(vs.Anas Platyrhynchos)and from 0.316 to 0.522(vs.A.Poecilorhyncha),respectively.The variance analysis showed no significant difference between the two groups of data,which indicated that both mallard and spot-billed ducks made contributions to domestic duck evolution.A dendrogram was constructed according to the D value.展开更多
Twelve fluorescence-labeled microsatellite markers were used to analyze the genetic diversity of 12 domestic duck breeds and 2 wild duck breeds to determine the relationship and origin of Chinese domestic duck breeds....Twelve fluorescence-labeled microsatellite markers were used to analyze the genetic diversity of 12 domestic duck breeds and 2 wild duck breeds to determine the relationship and origin of Chinese domestic duck breeds. Gene frequency, effective number of alleles （Ne）, expected heterozygosity （He）, polymorphism information contents （PIC）, inbreeding coefficient in population （Fis）, standard genetic distance （Ds）, and genetic distance （DA） were calculated by FSTAT and distance and phylogenetic analysis after the dates which were output from the Microsatellite-Toolkit software. Genetic distances between 12 domestic duck breeds and 2 wild duck breeds were analyzed by variance analysis. Unweighted pair group method with arithmetic mean （UPGMA） and phylogenetic trees used for cluster analysis were structured. The results indicated that 11 loci had medium- or high-level genetic diversity among the 12 loci, which could be efficiently used in the detection of the genetic parameters of each population. The values of He were 0.5414 to 0.7343, those of PIC proved similar, and those of Fis were 0.1101 to 0.3381 among all populations. All breeds were clustered into three groups by UPGMA phylogenetic trees. Banzui duck was clustered into a separate group. Differences of the DA were analysed by t-test. The results showed that difference in DA between the 12 domestic duck breeds and Lvtou duck and the Banzui duck were very significant （P〈0.01）, indicating that these 12 domestic duck breeds originated from Lvtou wild duck, but not Banzui duck.展开更多
文摘The amplified fragment length polymorphic(AFLP)technique was used to analyze the genome DNA polymorphism among 8 breeds of domestic ducks and 2 species of wild ducks.Nine of the 17 selected primers pairs gave reproducible polymorphic DNA amplification bands.The amplified bands ranged from 44 to 83 per primer pair.Of the 513 AFLP markers obtained,498 were polymorphic.The proportion of polymorphic loci was 97.1%.The genetic distance(D)and similarity coefficients(GS)were calcu-lated based on the polymorphic data.Between domestic ducks D ranged from 0.331 to 0.589,while between domes-tic ducks and the wild ducks,it ranged from 0.298 to 0.520(vs.Anas Platyrhynchos)and from 0.316 to 0.522(vs.A.Poecilorhyncha),respectively.The variance analysis showed no significant difference between the two groups of data,which indicated that both mallard and spot-billed ducks made contributions to domestic duck evolution.A dendrogram was constructed according to the D value.
基金the National Special Funds of System of Industrial Technologies for Modern Agriculture of China(nycytx-45-04)
文摘Twelve fluorescence-labeled microsatellite markers were used to analyze the genetic diversity of 12 domestic duck breeds and 2 wild duck breeds to determine the relationship and origin of Chinese domestic duck breeds. Gene frequency, effective number of alleles （Ne）, expected heterozygosity （He）, polymorphism information contents （PIC）, inbreeding coefficient in population （Fis）, standard genetic distance （Ds）, and genetic distance （DA） were calculated by FSTAT and distance and phylogenetic analysis after the dates which were output from the Microsatellite-Toolkit software. Genetic distances between 12 domestic duck breeds and 2 wild duck breeds were analyzed by variance analysis. Unweighted pair group method with arithmetic mean （UPGMA） and phylogenetic trees used for cluster analysis were structured. The results indicated that 11 loci had medium- or high-level genetic diversity among the 12 loci, which could be efficiently used in the detection of the genetic parameters of each population. The values of He were 0.5414 to 0.7343, those of PIC proved similar, and those of Fis were 0.1101 to 0.3381 among all populations. All breeds were clustered into three groups by UPGMA phylogenetic trees. Banzui duck was clustered into a separate group. Differences of the DA were analysed by t-test. The results showed that difference in DA between the 12 domestic duck breeds and Lvtou duck and the Banzui duck were very significant （P〈0.01）, indicating that these 12 domestic duck breeds originated from Lvtou wild duck, but not Banzui duck.
文摘利用高光谱遥感技术定量估测野鸭湖湿地植被含水量,对于监测和诊断野鸭湖湿地植被的生理状况及生长趋势具有重要意义,也能够为高光谱遥感影像在野鸭湖湿地植被含水量诊断中的实际应用提供理论依据和技术支持。采用Field Spec 3野外高光谱辐射仪,获取了野鸭湖典型湿地植被冠层和叶片的光谱,并测定了对应的含水量。以上述实测数据为基础,首先以芦苇为例初步探明了不同含水量水平下典型湿地植被冠层和叶片光谱反射率的响应模式,然后采用相关性及单变量线性与非线性拟合分析技术,从冠层和叶片两种层次,对不同尺度下的含水量与"三边"参数及高光谱植被指数进行了分析拟合,并采用交叉检验中的3K-CV方法对估算模型进行了测试和检验,确立了不同尺度下野鸭湖湿地植被含水量的定量监测模型。结果表明:(1)随着含水量水平的增加,芦苇冠层与叶片光谱在可见光波段(350—760 nm)和红外波段(760—2500 nm)的反射率均呈逐渐降低趋势。(2)不同尺度含水量与选取的光谱特征参数整体上相关性较强,与"三边"参数基本上都呈极显著相关,相关系数最大达到0.906;与高光谱指数全部呈极显著相关,相关系数最小为0.455,最大达到0.919,并通过选取不同尺度上相关性最佳的光谱特征参数,分别基于"三边"参数和高光谱植被指数构建了不同尺度下的含水量估算模型。其中,冠层尺度下,黄边面积(SDy)与SRWI(Simple Ratio Water Index)的估算效果最好,估算模型分别为y=-9.462x2-2.671x+0.608和y=0.219e1.010x;叶片尺度下,红边面积(SDr)与WI(Water Index)的估算效果最好,估算模型分别为y=0.562x+0.376和y=2.028x2-0.476x-1.009。通过3K-CV的交叉验证,不同尺度下的含水量估算模型均取得了较为理想的预测精度,预测精度的最小值为94.92%,最大值为97.06%,表明估测模型具有较高的可靠性与普适性。(3)高光谱植被指数与含水量拟合方程的拟合度相对高于"三边"参数与之建立方程的拟合度,说明多波段组合的光谱特征参数更适合含水量的判别。
