东昆仑成矿带是中国西部重大成(找)矿潜力的贵金属-有色金属成矿带。对东昆仑东段都兰县诺木洪南部产于下石炭统哈拉郭勒组(C1h)中的杏树沟金矿(化)点进行了初步研究,认为该金矿(化)点受近东西向韧脆性剪切构造的控制,矿化带内中酸性脉...东昆仑成矿带是中国西部重大成(找)矿潜力的贵金属-有色金属成矿带。对东昆仑东段都兰县诺木洪南部产于下石炭统哈拉郭勒组(C1h)中的杏树沟金矿(化)点进行了初步研究,认为该金矿(化)点受近东西向韧脆性剪切构造的控制,矿化带内中酸性脉岩发育,与构造蚀变岩型金矿有相似的特征,可以与东昆仑其它金矿对比,具有一定的成矿潜力。含矿围岩为一套火山碎屑岩-碎屑岩建造,矿化蚀变主要为褐铁矿化、孔雀石化和黄铁矿化。在围岩的灰岩夹层中发现大量珊瑚、腕足和腹足类化石,其中Siphonodendron asiatica Yabe et Hayasaka和Siphonodendron asiatica minor Minato这2种珊瑚化石是首次在哈拉郭勒组发现,这不仅丰富了哈拉郭勒组的化石组合类型,而且将杏树沟金矿(化)点的围岩时代进一步限定为早石炭世维宪期。展开更多
The prostate secretory protein of 94 amino acids （PSP94） has been shown to interact with cysteine-rich secretory protein 3 （CRISP-3） in human seminal plasma. Interestingly, PSP94 expression is redaced or lost in t...The prostate secretory protein of 94 amino acids （PSP94） has been shown to interact with cysteine-rich secretory protein 3 （CRISP-3） in human seminal plasma. Interestingly, PSP94 expression is redaced or lost in the majority of the prostate tumours, whereas CRISP-3 expression is upregulated in prostate cancer compared with normal prostate tissue. To obtain a better understanding of the individual roles these proteins have in prostate tumourigenesis and the functional relevance of their interaction, we ectopically expressed either PSP94 or CRISP-3 alone or PSP94 along with CRISP-3 in three prostate cell lines （PC3, WPE1-NB26 and LNCaP） and performed growth inhibition assays. Reverse transcription-polymerase chain reaction and Western blot analysis were used to screen prostate cell lines for PSP94 and CRISP-3 expression. Mammalian expression constructs for human PSP94 and CRISP-3 were also generated and the expression, localization and secretion of recombinant protein were assayed by transfection followed by Western blot analysis and immunofluorescence assay. The effect that ectopic expression of PSP94 or CRISP-3 had on cell growth was studied by clonogenic survival assay follokving transfection. To evaluate the effects of coexpression of the two proteins, stable clones of PC3 that expressed PSP94 were generated. They were subsequently transfected with a CRISP-3 expression construct and subjected to clonogenic survival assay. Our results showed that PSP94 and CRISP-3 could each induce growth inhibition in a cell line specific manner. Although the growth of CRISP-3-positive cell lines was inhibited by PSP94, growth inhibition mediated by CRISP-3 was not affected by the presence or absence of PSP94. This suggests that CRISP-3 may participate in PSP94-independent activities during prostate tumourigenesis.展开更多
文摘东昆仑成矿带是中国西部重大成(找)矿潜力的贵金属-有色金属成矿带。对东昆仑东段都兰县诺木洪南部产于下石炭统哈拉郭勒组(C1h)中的杏树沟金矿(化)点进行了初步研究,认为该金矿(化)点受近东西向韧脆性剪切构造的控制,矿化带内中酸性脉岩发育,与构造蚀变岩型金矿有相似的特征,可以与东昆仑其它金矿对比,具有一定的成矿潜力。含矿围岩为一套火山碎屑岩-碎屑岩建造,矿化蚀变主要为褐铁矿化、孔雀石化和黄铁矿化。在围岩的灰岩夹层中发现大量珊瑚、腕足和腹足类化石,其中Siphonodendron asiatica Yabe et Hayasaka和Siphonodendron asiatica minor Minato这2种珊瑚化石是首次在哈拉郭勒组发现,这不仅丰富了哈拉郭勒组的化石组合类型,而且将杏树沟金矿(化)点的围岩时代进一步限定为早石炭世维宪期。
文摘The prostate secretory protein of 94 amino acids （PSP94） has been shown to interact with cysteine-rich secretory protein 3 （CRISP-3） in human seminal plasma. Interestingly, PSP94 expression is redaced or lost in the majority of the prostate tumours, whereas CRISP-3 expression is upregulated in prostate cancer compared with normal prostate tissue. To obtain a better understanding of the individual roles these proteins have in prostate tumourigenesis and the functional relevance of their interaction, we ectopically expressed either PSP94 or CRISP-3 alone or PSP94 along with CRISP-3 in three prostate cell lines （PC3, WPE1-NB26 and LNCaP） and performed growth inhibition assays. Reverse transcription-polymerase chain reaction and Western blot analysis were used to screen prostate cell lines for PSP94 and CRISP-3 expression. Mammalian expression constructs for human PSP94 and CRISP-3 were also generated and the expression, localization and secretion of recombinant protein were assayed by transfection followed by Western blot analysis and immunofluorescence assay. The effect that ectopic expression of PSP94 or CRISP-3 had on cell growth was studied by clonogenic survival assay follokving transfection. To evaluate the effects of coexpression of the two proteins, stable clones of PC3 that expressed PSP94 were generated. They were subsequently transfected with a CRISP-3 expression construct and subjected to clonogenic survival assay. Our results showed that PSP94 and CRISP-3 could each induce growth inhibition in a cell line specific manner. Although the growth of CRISP-3-positive cell lines was inhibited by PSP94, growth inhibition mediated by CRISP-3 was not affected by the presence or absence of PSP94. This suggests that CRISP-3 may participate in PSP94-independent activities during prostate tumourigenesis.
