Recent studies have shown the potential of artificially synthesized conduits in the repair of peripheral nerve injury. Natural biopolymers have received much attention because of their biocompatibility. To investigate...Recent studies have shown the potential of artificially synthesized conduits in the repair of peripheral nerve injury. Natural biopolymers have received much attention because of their biocompatibility. To investigate the effects of novel electrospun absorbable poly(ε-caprolactone)/type Ⅰ collagen nanofiber conduits(biopolymer nanofiber conduits) on the repair of peripheral nerve injury, we bridged 10-mm-long sciatic nerve defects with electrospun absorbable biopolymer nanofiber conduits, poly(ε-caprolactone) or silicone conduits in Sprague-Dawley rats. Rat neurologica1 function was weekly evaluated using sciatic function index within8 weeks after repair. Eight weeks after repair, sciatic nerve myelin sheaths and axon morphology were observed by osmium tetroxide staining, hematoxylin-eosin staining, and transmission electron microscopy.S-100(Schwann cell marker) and CD4(inflammatory marker) immunoreactivities in sciatic nerve were detected by immunohistochemistry. In rats subjected to repair with electrospun absorbable biopolymer nanofiber conduits, no serious inflammatory reactions were observed in rat hind limbs, the morphology of myelin sheaths in the injured sciatic nerve was close to normal. CD4 immunoreactivity was obviously weaker in rats subjected to repair with electrospun absorbable biopolymer nanofiber conduits than in those subjected to repair with poly(ε-caprolactone) or silicone. Rats subjected to repair with electrospun absorbable biopolymer nanofiber conduits tended to have greater sciatic nerve function recovery than those receiving poly(ε-caprolactone) or silicone repair. These results suggest that electrospun absorbable poly(ε-caprolactone)/type Ⅰ collagen nanofiber conduits have the potential of repairing sciatic nerve defects and exhibit good biocompatibility. All experimental procedures were approved by Institutional Animal Care and Use Committee of Taichung Veteran General Hospital, Taiwan, China(La-1031218) on October 2, 2014.展开更多
Micromechanics aims mainly at establishing the quantitative relation between the macroscopic mechanical behavior and the microstructure of heterogeneous materials.
ln order to raise the tractive efficiency of a boat-typc tractor further, according to a point of view about reducing energy consumption by reducing the volume of thc blade cavity, an idea of four-footed walkiag boat-...ln order to raise the tractive efficiency of a boat-typc tractor further, according to a point of view about reducing energy consumption by reducing the volume of thc blade cavity, an idea of four-footed walkiag boat-type tractor is put forward. and a walking device with two feet and four small wheels is designed and manufactured. It can move on the rails of a paddy soil bin to simulate the tractive performance of a boat-type tractor in thc paddy field. Kinematics and dynam- ics of the walking device is analysed. Because it can ensure that the blade foot enters and leaves the soil vertically, and eliminates the functions of the soil compression and pick by the blade entirely, so the volume of the blade cavity can be reduced to a minimum and the efficiency of the walking sys- tem has been rasied considerably. Thus, it is feasible to adopt a four-footed walking mechanism in the boat-type tractor.展开更多
基金supported by grants from the Taichung Veterans General Hospital and Central Taiwan University of Science and Technology,No.TCVGH-CTUST1047701(to CCS and BSL)Taichung Veterans General Hospital,No.TCVGH-1034907C(to CCS),Taiwan,China
文摘Recent studies have shown the potential of artificially synthesized conduits in the repair of peripheral nerve injury. Natural biopolymers have received much attention because of their biocompatibility. To investigate the effects of novel electrospun absorbable poly(ε-caprolactone)/type Ⅰ collagen nanofiber conduits(biopolymer nanofiber conduits) on the repair of peripheral nerve injury, we bridged 10-mm-long sciatic nerve defects with electrospun absorbable biopolymer nanofiber conduits, poly(ε-caprolactone) or silicone conduits in Sprague-Dawley rats. Rat neurologica1 function was weekly evaluated using sciatic function index within8 weeks after repair. Eight weeks after repair, sciatic nerve myelin sheaths and axon morphology were observed by osmium tetroxide staining, hematoxylin-eosin staining, and transmission electron microscopy.S-100(Schwann cell marker) and CD4(inflammatory marker) immunoreactivities in sciatic nerve were detected by immunohistochemistry. In rats subjected to repair with electrospun absorbable biopolymer nanofiber conduits, no serious inflammatory reactions were observed in rat hind limbs, the morphology of myelin sheaths in the injured sciatic nerve was close to normal. CD4 immunoreactivity was obviously weaker in rats subjected to repair with electrospun absorbable biopolymer nanofiber conduits than in those subjected to repair with poly(ε-caprolactone) or silicone. Rats subjected to repair with electrospun absorbable biopolymer nanofiber conduits tended to have greater sciatic nerve function recovery than those receiving poly(ε-caprolactone) or silicone repair. These results suggest that electrospun absorbable poly(ε-caprolactone)/type Ⅰ collagen nanofiber conduits have the potential of repairing sciatic nerve defects and exhibit good biocompatibility. All experimental procedures were approved by Institutional Animal Care and Use Committee of Taichung Veteran General Hospital, Taiwan, China(La-1031218) on October 2, 2014.
文摘Micromechanics aims mainly at establishing the quantitative relation between the macroscopic mechanical behavior and the microstructure of heterogeneous materials.
文摘ln order to raise the tractive efficiency of a boat-typc tractor further, according to a point of view about reducing energy consumption by reducing the volume of thc blade cavity, an idea of four-footed walkiag boat-type tractor is put forward. and a walking device with two feet and four small wheels is designed and manufactured. It can move on the rails of a paddy soil bin to simulate the tractive performance of a boat-type tractor in thc paddy field. Kinematics and dynam- ics of the walking device is analysed. Because it can ensure that the blade foot enters and leaves the soil vertically, and eliminates the functions of the soil compression and pick by the blade entirely, so the volume of the blade cavity can be reduced to a minimum and the efficiency of the walking sys- tem has been rasied considerably. Thus, it is feasible to adopt a four-footed walking mechanism in the boat-type tractor.
