Five new toosendanin limonoids with highly oxidative furan ring walsurobustones A-D(1-4),and one new furan ring degraded limonoid walsurobustone E(5)together with one known compound toonapubesic acid B(6)were isolated...Five new toosendanin limonoids with highly oxidative furan ring walsurobustones A-D(1-4),and one new furan ring degraded limonoid walsurobustone E(5)together with one known compound toonapubesic acid B(6)were isolated from the leaves of Walsura robusta.Their structures were elucidated by NMR and MS data.Especially,the absolute configuration of toonapubesic acid B(6)was confirmed by X-ray diffraction study.Compounds 1-6 exhibited good cytotoxicity against the cancer cell lines HL-60,SMMC-7721,A-549,MCF-7,and SW480.展开更多
Screening active natural products, rapid identification, and accurate isolation are of great important for modern natural lead compounds discovery1. We hereby reported the isolation of seven new neotecleanin-type limo...Screening active natural products, rapid identification, and accurate isolation are of great important for modern natural lead compounds discovery1. We hereby reported the isolation of seven new neotecleanin-type limonoids(1–7), seven new limonoids with 5-oxatricyclo[5.4.0.11,4]hendecane ring system(8–14), and two new precursors(15–16) together with four known limonoids(17–20) from the root barks of Walsura robusta. Their structures, including their absolute configurations, were elucidated based on analyses of HR-ESI-MS, 1D/2D NMR, ECD spectrum calculations and singlecrystal X-ray diffraction techniques. Compounds 2, 8, 9, 11, 13, 14, 18 showed significant anti-inflammatory activities in LPS-induced RAW 264.7 cell line, BV2 microglial cells, and Propionibacterium acnes-stimulated THP-1 human monocytic cells. Walrobsin M(11) exhibited anti-inflammatory activity with IC50 value of 7.9670.36 μmol/L, and down-regulated phosphorylation levels of ERK and p38 in a dose-dependent manner.展开更多
基金the National Natural Science Foundation of China(U1812403 to X.-J.Hao and 22177050 to M.Cao)Project of Yunnan Characteristic Plant Screening and R&D Service CXO Platform(2022YKZY001)+1 种基金Foundation of Central Asian Drug Discovery and Development Center of Chinese Academy of Sciences(CAM202103,China)Youth Innovation Promotion Association CAS to M.Cao(2022-2026).
文摘Five new toosendanin limonoids with highly oxidative furan ring walsurobustones A-D(1-4),and one new furan ring degraded limonoid walsurobustone E(5)together with one known compound toonapubesic acid B(6)were isolated from the leaves of Walsura robusta.Their structures were elucidated by NMR and MS data.Especially,the absolute configuration of toonapubesic acid B(6)was confirmed by X-ray diffraction study.Compounds 1-6 exhibited good cytotoxicity against the cancer cell lines HL-60,SMMC-7721,A-549,MCF-7,and SW480.
基金Financial support for this study by the National Natural Science Foundation of China (31470416, China)the Outstanding Youth Fund of the Basic Research Program of Jiangsu Province (BK20160077, China)+1 种基金the Program for Changjiang Scholars and Innovative Research Team in University (IRT_15R63, China)the "Double First-Class" University project (CPU2018GY08, China)
文摘Screening active natural products, rapid identification, and accurate isolation are of great important for modern natural lead compounds discovery1. We hereby reported the isolation of seven new neotecleanin-type limonoids(1–7), seven new limonoids with 5-oxatricyclo[5.4.0.11,4]hendecane ring system(8–14), and two new precursors(15–16) together with four known limonoids(17–20) from the root barks of Walsura robusta. Their structures, including their absolute configurations, were elucidated based on analyses of HR-ESI-MS, 1D/2D NMR, ECD spectrum calculations and singlecrystal X-ray diffraction techniques. Compounds 2, 8, 9, 11, 13, 14, 18 showed significant anti-inflammatory activities in LPS-induced RAW 264.7 cell line, BV2 microglial cells, and Propionibacterium acnes-stimulated THP-1 human monocytic cells. Walrobsin M(11) exhibited anti-inflammatory activity with IC50 value of 7.9670.36 μmol/L, and down-regulated phosphorylation levels of ERK and p38 in a dose-dependent manner.
