A study on the simulation of carbon and water fluxes of Dangxiong alpine meadow and its response to climate change

The alpine meadow ecosystem accounts for 27%of the total area of the Tibetan Plateau and is also one of the most important vegetation types.The Dangxiong alpine meadow ecosystem,located in the south-central part of the Tibetan Plateau,is a typical example.To understand the carbon and water fluxes,water use efficiency(WUE),and their responses to future climate change for the alpine meadow ecosystem in the Dangxiong area,two parameter estimation methods,the Model-independent Parameter Estimation(PEST)and the Dynamic Dimensions Search(DDS),were used to optimize the Biome-BGC model.Then,the gross primary productivity(GPP)and evapotranspiration(ET)were simulated.The results show that the DDS parameter calibration method has a better performance.The annual GPP and ET show an increasing trend,while the WUE shows a decreasing trend.Meanwhile,ET and GPP reach their peaks in July and August,respectively,and WUE shows a“dual-peak”pattern,reaching peaks in May and November.Furthermore,according to the simulation results for the next nearly 100 years,the ensemble average GPP and ET exhibit a significant increasing trend,and the growth rate under the SSP5–8.5 scenario is greater than that under the SSP2–4.5 scenario.WUE shows an increasing trend under the SSP2–4.5 scenario and a significant increasing trend under the SSP5–8.5 scenario.This study has important scientific significance for carbon and water cycle prediction and vegetation ecological protection on the Tibetan Plateau.

Yields,growth and water use under chemical topping in relations to row configuration and plant density in drip-irrigated cotton

Background Water deficit is an important problem in agricultural production in arid regions.With the advent of wholly mechanized technology for cotton planting in Xinjiang,it is important to determine which planting mode could achieve high yield,fiber quality and water use efficiency(WUE).This study aimed to explore if chemical topping affected cotton yield,quality and water use in relation to row configuration and plant densities.Results Experiments were carried out in Xinjiang China,in 2020 and 2021 with two topping method,manual topping and chemical topping,two plant densities,low and high,and two row configurations,i.e.,76 cm equal rows and 10+66 cm narrow-wide rows,which were commonly applied in matching harvest machine.Chemical topping increased seed cotton yield,but did not affect cotton fiber quality comparing to traditional manual topping.Under equal row spacing,the WUE in higher density was 62.4%higher than in the lower one.However,under narrow-wide row spacing,the WUE in lower density was 53.3%higher than in higher one(farmers’practice).For machine-harvest cotton in Xinjiang,the optimal row configuration and plant density for chemical topping was narrow-wide rows with 15 plants m-2 or equal rows with 18 plants m-2.Conclusion The plant density recommended in narrow-wide rows was less than farmers’practice and the density in equal rows was moderate with local practice.Our results provide new knowledge on optimizing agronomic managements of machine-harvested cotton for both high yield and water efficient.

Effects of thinning and understory removal on water use efficiency of Pinus massoniana:evidence from photosynthetic capacity and stable carbon isotope analyses

Understanding the relationship between forest management and water use efficiency(WUE)is important for evaluating forest adaptability to climate change.However,the effects of thinning and understory removal on WUE and its key controlling processes are not well understood,which limits our comprehension of the physiological mechanisms of various management practices.In this study,four forest management measures(no thinning:NT;understory removal:UR;light thinning:LT;and heavy thinning:HT)were carried out in Pinus massoniana plantations in a subtropical region of China.Photosynthetic capacity and needle stable carbon isotope composition(δ^(13)C)were measured to assess instantaneous water use efficiency(WUE_(inst))and long-term water use efficiency(WUE_(i)).Multiple regression models and structural equation modelling(SEM)identified the effects of soil properties and physiological performances on WUE_(inst)and WUE_(i).The results show that WUE_(inst)values among the four treatments were insignificant.However,compared with the NT stand(35.8μmol·mol^(-1)),WUE_(i)values significantly increased to 41.7μmol·mol^(-1)in the UR,50.1μmol·mol^(-1)in the LT and 46.6μmol·mol^(-1)in HT treatments,largely explained by photosynthetic capacity and soil water content.Understory removal did not change physiological performance(needle water potential and photosynthetic capacity).Thinning increased the net photosynthetic rate(A_n)but not stomatal conductance(g_s)or predawn needle water potential(ψ_(pd)),implying that the improvement in water use efficiency for thinned stands was largely driven by radiation interception than by soil water availability.In general,thinning may be an appropriate management measure to promote P.massoniana WUE to cope with seasonal droughts under future extreme climates.

Water and nitrogen footprint assessment of integrated agronomic practice management in a summer maize cropping system

The footprints of water and nitrogen(WF and NF)provide a comprehensive overview of the type and quantity of water consumption and reactive nitrogen(Nr)loss in crop production.In this study,a field experiment over two years(2019 and 2020)compared three integrated agronomic practice management(IAPM)systems:An improved management system(T2),a high-yield production system(T3),and an integrated soil-crop management system(ISCM)using a local smallholder farmer’s practice system(T1)as control,to investigate the responses of WF,Nr losses,water use efficiency(WUE),and nitrogen use efficiency(NUE)to IAPM.The results showed that IAPM optimized water distribution and promoted water use by summer maize.The evapotranspiration over the whole maize growth period of IAPM increased,but yield increased more,leading to a significant increase in WUE.The WUE of the T2,T3,and ISCM treatments was significantly greater than in the T1 treatment,in 2019 and 2020respectively,by 19.8-21.5,31.8-40.6,and 34.4-44.6%.The lowest WF was found in the ISCM treatment,which was 31.0%lower than that of the T1 treatment.In addition,the ISCM treatment optimized soil total nitrogen(TN)distribution and significantly increased TN in the cultivated layer.Excessive nitrogen fertilizer was applied in treatment T3,producing the highest maize yield,and resulting in the highest Nr losses.In contrast,the ISCM treatment used a reduced nitrogen fertilizer rate,sacrificing grain yield partly,which reduced Nr losses and eventually led to a significant increase in nitrogen use efficiency and nitrogen recovery.The Nr level in the ISCM treatment was34.8%lower than in the T1 treatment while NUE was significantly higher than in the T1 treatment by 56.8-63.1%in2019 and 2020,respectively.Considering yield,WUE,NUE,WF,and NF together,ISCM should be used as a more sustainable and clean system for sustainable production of summer maize.

Regulation effects of water and nitrogen on yield,water,and nitrogen use efficiency of wolfberry

Wolfberry(Lycium barbarum L.)is important for health care and ecological protection.However,it faces problems of low productivity and resource utilization during planting.Exploring reasonable models for water and nitrogen management is important for solving these problems.Based on field trials in 2021 and 2022,this study analyzed the effects of controlling soil water and nitrogen application levels on wolfberry height,stem diameter,crown width,yield,and water(WUE)and nitrogen use efficiency(NUE).The upper and lower limits of soil water were controlled by the percentage of soil water content to field water capacity(θ_(f)),and four water levels,i.e.,adequate irrigation(W0,75%-85%θ_(f)),mild water deficit(W1,65%-75%θ_(f)),moderate water deficit(W2,55%-65%θ_(f)),and severe water deficit(W3,45%-55%θ_(f))were used,and three nitrogen application levels,i.e.,no nitrogen(N0,0 kg/hm^(2)),low nitrogen(N1,150 kg/hm^(2)),medium nitrogen(N2,300 kg/hm^(2)),and high nitrogen(N3,450 kg/hm^(2))were implied.The results showed that irrigation and nitrogen application significantly affected plant height,stem diameter,and crown width of wolfberry at different growth stages(P<0.01),and their maximum values were observed in W1N2,W0N2,and W1N3 treatments.Dry weight per plant and yield of wolfberry first increased and then decreased with increasing nitrogen application under the same water treatment.Dry weight per hundred grains and dry weight percentage increased with increasing nitrogen application under W0 treatment.However,under other water treatments,the values first increased and then decreased with increasing nitrogen application.Yield and its component of wolfberry first increased and then decreased as water deficit increased under the same nitrogen treatment.Irrigation water use efficiency(IWUE,8.46 kg/(hm^(2)·mm)),WUE(6.83 kg/(hm^(2)·mm)),partial factor productivity of nitrogen(PFPN,2.56 kg/kg),and NUE(14.29 kg/kg)reached their highest values in W2N2,W1N2,W1N2,and W1N1 treatments.Results of principal component analysis(PCA)showed that yield,WUE,and NUE were better in W1N2 treatment,making it a suitable water and nitrogen management mode for the irrigation area of the Yellow River in the Gansu Province,China and similar planting areas.

Changes in Water Use Efficiency Caused by Climate Change,CO_(2) Fertilization,and Land Use Changes on the Tibetan Plateau

Terrestrial ecosystem water use efficiency(WUE)is an important indicator for coupling plant photosynthesis and transpiration,and is also a key factor linking the carbon and water cycles between the land and atmosphere.However,under the combination of climate change and human intervention,the change in WUE is still unclear,especially on the Tibetan Plateau(TP).Therefore,satellite remote sensing data and process-based terrestrial biosphere models(TBMs)are used in this study to investigate the spatiotemporal variations of WUE over the TP from 2001 to 2010.Then,the effects of land use and land cover change(LULCC)and CO_(2) fertilization on WUE from 1981-2010 are assessed using TBMs.Results show that climate change is the leading contributor to the change in WUE on the TP,and temperature is the most important factor.LULCC makes a negative contribution to WUE(-20.63%),which is greater than the positive contribution of CO_(2) fertilization(11.65%).In addition,CO_(2) fertilization can effectively improve ecosystem resilience on the TP.On the northwest plateau,the effects of LULCC and CO_(2) fertilization on WUE are more pronounced during the driest years than the annual average.These findings can help researchers understand the response of WUE to climate change and human activity and the coupling of the carbon and water cycles over the TP.

Arbuscular mycorrhizal fungi improve biomass, photosynthesis, and water use efficiency of Opuntia ficus-indica (L.) Miller under different water levels

Opuntia ficus-indica(L.)Miller is a CAM(crassulacean acid metabolism)plant with an extraordinary capacity to adapt to drought stress by its ability to fix atmospheric CO_(2) at nighttime,store a significant amount of water in cladodes,and reduce root growth.Plants that grow in moisture-stress conditions with thick and less fine root hairs have a strong symbiosis with arbuscular mycorrhizal fungi(AMF)to adapt to drought stress.Water stress can limit plant growth and biomass production,which can be rehabilitated by AMF association through improved physiological performance.The objective of this study was to investigate the effects of AMF inoculations and variable soil water levels on the biomass,photosynthesis,and water use efficiency of the spiny and spineless O.ficus-indica.The experiment was conducted in a greenhouse with a full factorial experiment using O.ficus-indica type(spiny or spineless),AMF(presence or absence),and four soil water available(SWA)treatments through seven replications.Water treatments applied were 0%–25%SWA(T1),25%–50%SWA(T2),50%–75%SWA(T3),and 75%–100%SWA(T4).Drought stress reduced biomass and cladode growth,while AMF colonization significantly increased the biomass production with significant changes in the physiological performance of O.ficus-indica.AMF presence significantly increased biomass of both O.ficus-indica plant types through improved growth,photosynthetic water use efficiency,and photosynthesis.The presence of spines on the surface of cladodes significantly reduced the rate of photosynthesis and photosynthetic water use efficiency.Net photosynthesis,photosynthetic water use efficiency,transpiration,and stomatal conductance rate significantly decreased with increased drought stress.Under drought stress,some planted mother cladodes with the absence of AMF have not established daughter cladodes,whereas AMF-inoculated mother cladodes fully established daughter cladodes.AMF root colonization significantly increased with the decrease of SWA.AMF caused an increase in biomass production,increased tolerance to drought stress,and improved photosynthesis and water use efficiency performance of O.ficus-indica.The potential of O.ficus-indica to adapt to drought stress is controlled by the morpho-physiological performance related to AMF association.

Controlled drainage in the Nile River delta of Egypt:a promising approach for decreasing drainage off-site effects and enhancing yield and water use efficiency of wheat

North Africa is one of the most regions impacted by water shortage.The implementation of controlled drainage(CD)in the northern Nile River delta of Egypt is one strategy to decrease irrigation,thus alleviating the negative impact of water shortage.This study investigated the impacts of CD at different levels on drainage outflow,water table level,nitrate loss,grain yield,and water use efficiency(WUE)of various wheat cultivars.Two levels of CD,i.e.,0.4 m below the soil surface(CD-0.4)and 0.8 m below the soil surface(CD-0.8),were compared with subsurface free drainage(SFD)at 1.2 m below the soil surface(SFD-1.2).Under each drainage treatment,four wheat cultivars were grown for two growing seasons(November 2018–April 2019 and November 2019–April 2020).Compared with SFD-1.2,CD-0.4 and CD-0.8 decreased irrigation water by 42.0%and 19.9%,drainage outflow by 40.3%and 27.3%,and nitrate loss by 35.3%and 20.8%,respectively.Under CD treatments,plants absorbed a significant portion of their evapotranspiration from shallow groundwater(22.0%and 8.0%for CD-0.4 and CD-0.8,respectively).All wheat cultivars positively responded to CD treatments,and the highest grain yield and straw yield were obtained under CD-0.4 treatment.Using the initial soil salinity as a reference,the soil salinity under CD-0.4 treatment increased two-fold by the end of the second growing season without negative impacts on wheat yield.Modifying the drainage system by raising the outlet elevation and considering shallow groundwater contribution to crop evapotranspiration promoted water-saving and WUE.Different responses could be obtained based on the different plant tolerance to salinity and water stress,crop characteristics,and growth stage.Site-specific soil salinity management practices will be required to avoid soil salinization due to the adoption of long-term shallow groundwater in Egypt and other similar agroecosystems.

Seasonality of PSII thermostability and water use e ffi ciency of in situ mountainous Norway spruce(Picea abies)

The stability of monocultural,even-aged spruce forests at lower altitudes in Central Europe is seriously threatened by the prospects of global climate change.The thermostability and water use efficiency of their photo synthetic apparatus might play a vital role in their successful acclimation.In this study,photo systemⅡ(PSⅡ)performance(OJIP transient,rapid light curves)and thermostability were analyzed in Norway spruce(Picea abies(L.)Karst.)throughout the growing season of the exceptionally warm year 2018(May-September)in the Western Carpathians,Slovakia.These measurements were accompanied by analysis of pigment concentrations in the needles.In addition,gas-exchange temperature curves were produced weekly from June until September to obtain intrinsic water use efficiencies.At the beginning of the growing season,needles exposed to heat stress showed significantly higher basal fluorescence and lower quantum yield,performance index,critical temperature thresholds of PSII inactivation and nonphotochemical yield in comparison to other months.The overall thermostability(heat-resistance)of PSII peaked in July and August,reflected in the lowest basal fluorescence and the highest quantum yield of PSII,critical temperature thresholds and yield of non-photochemical quenching under heat stress.Additionally,the ratio between chlorophyll and carotenoids was the highest in August and had a positive impact on PSII thermostability.Moreover,the high-temperature intrinsic water use efficiency was significantly higher during July and August than in June.Results show that15-year-old trees of Picea abies at 840 m a.s.l.exhibited acclimative seasonal responses of PSII thermostability and intrinsic water use efficiency during an exceptionally warm year.Our results suggest that mountainous P.abies at lower altitudes can acclimate their photosynthetic apparatus to higher temperatures during summer.

Effect of Rural Sewage Irrigation Regime on Water-Nitrogen Utilization and Crop Growth of Paddy Rice in Southern China

Reclaimed water irrigation has become an effective mean to alleviate the contradiction between water availability and its consumption worldwide.In this study,three types of irrigation water sources(rural sewage’s primary treated water R1 and secondary treated water R2,and river water R3)meeting the requirements of water quality for farmland irrigation were selected,and three types of irrigation water levels(low water levelW1 of 0–80 mm,medium water level W2 of 0–100 mm,and high water level W3 of 0–150 mm)were adopted to carry out research on the influence mechanismS of different irrigation water sources and water levels on water and nitrogen use and crop growth in paddy field.The water quantity indicators(irrigation times and irrigation volume),soil ammonium nitrogen(NH4+-N)and nitrate nitrogen(NO3−-N),rice yield indicators(thousand-grain weight,the number of grains per spike,and the number of effective spikes),and quality indicators(the amount of protein,amylose,vitamin C,nitrate and nitrite content)of rice were measured.The results showed that,the average irrigation volume under W3 was 2.4 and 1.9 times of that under W1 and W2,respectively.Compared with R3,the peak consumption of rice was lagged behind under R1 and R2,and the nitrogen form in 0–40 cm soil layers under rural sewage irrigation was mainly NH4+-N.The changes of NO3−-N and NH4+-N in the 0–40 cm soil layer showed the trend of declining and then increasing.The water level control only had a significant effect on the change of NO3−-N in the 60–80 cm soil layer.Both irrigation water use efficiency and crop water use efficiency were gradually reduced with the increase of field water level control.The nitrogen utilization efficiency under rural sewage irrigation was significantly higher than that under R3.Compared with the R3,rural sewage irrigation could significantly increase the yield of rice,and as the field water level rose,the effect of yield promotion was more obvious.It was noteworthy that the grain of rice under R1 monitored the low nitrate and nitrite content,but no nitrate and nitrite was discovered under R2 and R3.Therefore,reasonable rural sewage irrigation(R2)and medium water level(W2)were beneficial to improve nitrogen utilization efficiency,crop yield and crop quality promotion.

Response of Growth and Water Use Efficiency of Spring Wheat to Whole Season CO_2 Enrichment and Drought

Whole_growing season pot experiments were conducted to examine the response of growth and water use efficiency ( WUE ) of spring wheat ( Triticum aestivum L. cv. Gaoyuan 602) to CO 2 enrichment. Wheat plants were grown in open_top chambers (OTCs) subject to two concentrations of CO 2 ()(350 and 700 μL/L, hereafter 'ambient' and 'elevated' respectively) and three soil water levels (80%, 60% and 40% field water capacity ( FWC ), hereafter 'high soil moisture', 'medium soil moisture' and 'low soil moisture' respectively). Elevated CO 2 greatly increased leaf net photosynthesis ( Pn ) at all three soil water levels. The Pn of plants growing under elevated was 22% lower than that of plants growing at ambient when measured with the same (700 μL/L). Plant growth was enhanced by elevated throughout the growing season, with an increase of 14.8% in shoot dry weight at harvest under high soil moisture, and leaf area was increased by about 20% at all three soil water levels. Elevated in combination with high soil moisture increased the ratio of plant shoot dry weight to height by 15.7%, while this ratio was decreased by over 50% when plants were subject to drought. Elevated also increased the water use efficiency of wheat, mainly due to decreases in transpiration and cumulative consumption of water, and an increase in shoot dry weight, with the biggest value of 30% occurring at high soil water moisture level. Compared to high soil moisture, drought decreased shoot dry weight by 72% under ambient , and by 76% under elevated . Similarly, drought also reduced WUE by 19% under ambient , and 23% under elevated . Our results indicate that: (1) elevated can increase the photosynthetic rates, growth and WUE of wheat plants; (2) long_term exposure to high may result in lower photosynthetic capacity; (3) high stimulates plants lateral growth more than vertical growth; (4) the effects of CO 2 enrichment on plants depend on soil water status, with plants benefiting more from CO 2 enrichment if sufficient water is supplied; and (5) drought may cause relatively more reduction in plant growth and WUE under future elevated conditions.

Variations in Composition and Water Use Efficiency of Plant Functional Groups Based on Their Water Ecological Groups in the Xilin River Basin

Major plant species in the Xilin River Basin were grouped into six plant functional groups (PFGs) based on their water ecological groups: xerophytes, mesoxerophytes, xeromesophytes, mesophytes, hygromesophytes and hygrophytes. We surveyed the composition, delta(13)C values and proline concentration of PFGs in eight different plant communities along a soil moisture gradient. Results show that: (1) PFGs occurred variously in eight steppe communities with different soil moisture status. In wetter habitats, hygromesophytes and hygrophytes were more abundant and accounted for the majority of aboveground biomass, whereas xerophytes and mesoxerophytes became more conspicuous in dryer habitats; (2) the numerical order of the mean delta(13)C values of PFGs is as follows: xerophytes (-26.38parts per thousand) = mesoxerophytes (-26.51parts per thousand) > xeromesophytes (-27.02parts per thousand) > mesophytes (-27.56parts per thousand) = hygromesophytes and hygrophytes (-27.80parts per thousand); (3) xerophytes maintained relative higher delta(13)C values and water use efficiency (WUE) in habitats of different water availability, whereas delta(13)C values of xeromesophytes were more sensitive to change in soil water availability; (4) From xerophytes to hygrophytes, their proline content markedly increased. Significantly positive correlations existed between proline and biomass or delta(13)C values of different water ecological groups.

Species- and Habitat-variability of Photosynthesis, Transpiration and Water Use Efficiency of Different Plant Species in Maowusu Sand Area

Photosynthesis ( P n ), transpiration ( E ) and water use efficiency ( WUE ) of more than 66 arid sand species from different environmental habitats, shifting sand dune, fixed sand dune, lowland and wetland in the Maowusu Sand Area were analyzed and the relation among these characteristics and the resource utilization efficiency, taxonomic categories and growth forms of the species were assessed. The results showed that species from Chenopodiaceae, Gramineae, Leguminosae which possessed the C 4 photosynthesis pathway, or C 3 pathway and also with nitrogen_fixation capacities had higher or the highest P n values, i.e., 20～30 μmol CO 2·m -2 ·s -1 , while that of evergreen shrub of Pinaceae had the lowest P n values, i.e., 0～5 μmol CO 2·m -2 ·s -1 . Those species from Compositae, Scrophulariaceae, and Gramineae with C 3 pathway but no N_fixation capacity had the highest E rates, i.e., 20～30 mmol H 2O·m -2 ·s -1 and again the evergreen shrub together with some species from Salicaceae and Compositae had the lowest E rates, i.e., 0～5 mmol H 2O·m -2 ·s -1 . Species from Leguminosae, Gramineae and Chenopodiaceae with C 4 pathway or C 3 pathway with N_fixation capacity, both shrubs and grasses, generally had higher WUE . However, even the physiological traits of the same species were habitat_ and season_specific. The values of both P n and E in late summer were much higher than those in early summer, with average increases of 26%, 40% respectively in the four habitats. WUE in late summer was, however, 12% lower. Generally, when the environments became drier as a result of habitats changed, i.e., in the order of wetland, lowland, fixed sand dune and shifting sand dune, P n and E decreased but WUE increased.

Water Use of Leymus chinensis Community

Soil moisture of Leymus chinensis (Trin.) Tzvel. community has obviously stratified phenomena: the layer (0-40 cm) in which roots are concentrically distributed is directly influenced by precipitation and evapotranspiration. It can be called interaction layer of precipitation and evapotranspiration. The layer (40-120 cm), where water-storage capacity exchange lagged exchange of the root-layer water-storage capacity and the community evapotranspiration, can be called major water-storage layer. The layer (under 120 cm) can be called water relatively stable/balanced layer. The year 1996 was a normal flow year, and soil water had a surplus of 18 mm at the end of the growing season. The year 1998 was a high flow year, because leakage took place under continuous heavy rainfall, soil water had a deficit of 15 mm at the end of the growing season. Transpiration to evapotranspiration ( T/ET) value reflected not only the luxuriance degree of the community, but also the water use regime of the environmental resources. T/ET value was low (0.5) in May 1998, reaching 0.7 in June, then decreasing to 0.6 in July, due to the impact of rainfall inclining, while August reached the maximum (0.9), and September decreased to 0.6. Water use efficiency (WUE) was mainly restricted by the growing rate of plants under sufficient water condition (1998). Its seasonal changes were coincident with the grand period of growth of the plants. When both meanings of WUE and T/ET were analyzed profoundly, the concept of evapotranspiration efficiency (ETE) which can all-side reflect utilization regime of the environmental water resources was advanced.

Effects of Different Tillage Techniques on Yield and Water Use Efficiency in Broomcorn Millet

[Objective] The effects of different tillage techniques on dry matter accu- mulation, soil water content, water use efficiency and yield of broomcom millet were studied. [Method] With Jinsu 9 as an experiment material, the effects of deep tillage, traditional tillage and no tillage and rotary tillage on dry matter accumulation, soil water content, water use efficiency and yield of broomcom millet were investi- gated. [Result] Dry matter accumulation rate and accumulated amount were signifi- cantly higher in the deep tillage, no tillage and rotary tillage treatments than in the conventional tillage treatment, and the highest in the deep tillage treatment. The soil water content of the deep tillage treatment at 0-100 cm was higher than those of other tillage techniques, deep tillage also exhibited the highest soil water storage, and water use efficiency values were in order of deep tillage〉rotary tillage〉no tillage〉conventional tillage. The deep tillage treatment also showed the highest grain weight per spike, 1 000-grain weight and yield, while conventional tillage exhibited the lowest values, indicating that deep tillage is most beneficial to improvement of yield and water use efficiency of broomcom millet. [Conclusion] This study provides a scientific basis for water use efficiency of broomcorh millet in its main producing areas.

Responses of the seedlings of five dominant tree species in Changbai Mountain to soil water stress

Soil water stress was studied on the potted seedlings of five dominant tree species (Pinus koraienes Sieb.et Zucc., Fraxinus mandshurica Rupr., Juglans mandshurica Maxim, Tilia amurensis Rupr. and Quercus mongolica Fisch.ex Turcz) from the broadleaved/Korean pine forest in Changbai Mountain. Leaf growth, water transpiration and photosynthesis were compared for each species under three soil moisture conditions: 85%-100% (high water, CK), 65%-85% (Medium water, MW) and 45%-65% (low water, LW) of 37.4% water-holding capacity in field. The results showed that the characteristic of typical drought-resistance of the leaves is significantly developed. The net photosynthetic rate and water use efficiency of Fraxinus mandshurica were higher in MW than those in CK. But for the other four species, the net photosynthetic rate and water use efficiency in CK were lower than those in MW and LW. The transpiration rate responding to soil moistures varied from species to species.

Research Advance on Influencing Factors of Crop Water Use Efficiency

The research advance on the influencing factors of crop water use effi-ciency （WUE） was reviewed in this paper. Based on the discussion on the conno-tation of crop WUE, the influencing factors of crop WUE, such as crop, environ-ment, chemicals, cultivation measures, cropping systems, etc, were elaborated. A-mong them, the species and varieties of crop, soil and chemicals were discussed in detail.

Suitable Date of Seeding, Planting Density and Water Use Efficiency for Propagation of Stock Seed Potato in Mountainous Region of Southwest Sichuan

[Objective] The experiment was conducted to study suitable date of seed- ing and density of spring potato at the stock breeding base in Ebian County at an elevation of 1 200 to 1 500 m. [Methods] Virus-free Potato ＂Chuanyu 13＂ was used as material to study the effects of date of seeding and density on growing period, germination rate, yield and water use efficiency of spring potato in the field. [Result] With the postponement of date of seeding, the days from sowing to germination shortened, while the germination rate, the number of tubers per plant, the number of middle and small tubers in a group, yield and water use efficiency all increased. Planting density had no effects on the days from sowing to germination and the ger- mination rate, while the number of tubers per ptant, the number of middle and small tubers in a group, yield and water use efficiency increased significantly along with the increasing planting density. [Conclusion] At an elevation of 1 200 m to 1 250 m in Ebian County, the suitable date of seeding for potato was from February 9 to March 1, and the suitable planting density was 12×10^4 plants per hm^2, however, in the optimum planting density has not been found so that it needs further research,

Stable Isotope Technique——An Advanced Technology in Ascertaining Plant-Water Relations

Some key terms and relevant standard related with stable isotope tech- nique were introduced, and the determination of carbon, hydrogen and oxygen, the three stable isotopes in water, as well as their isotope effect was discussed. Appli- cations and advances of stable isotopes were stated mainly from plant water source, water use efficiency, the most active water adsorption region in root system and water resources utilization differences among different species in plant communi- ty. In addition, some existing problems in investigating the plant-water relations by using stable isotope technique were put forward. In this study, we aimed to promote the sustainable development of stable isotope technique in the research of plant water utilization and in the field of ecology in China.

Rice Cultivation under Film Mulching Can Improve Soil Environment and Be Beneficial for Rice Production in China

Rice cultivation under film mulching is an integrated management technology that can conserve water, increase soil temperature, improve yield, and enhance water and nitrogen use efficiencies. Despite these advantages, the system does have its drawbacks, such as soil organic matter reduction and microplastic pollution, which impede the widespread adoption of film mulching cultivation in China. Nonetheless, the advent of degradable film, controlled-release fertilizer, organic fertilizer, and film mulching machinery is promoting the development of rice film mulching cultivation. This review outlines the impact of rice cultivation under film mulching on soil moisture, soil temperature, soil fertility, greenhouse gas emissions, weed control, and disease and pest management. It also elucidates the mechanism of changes in rice growth, yield and quality, water use efficiency, and nitrogen use efficiency. This paper incorporates a review of published research articles and discusses some uncertainties and shortcomings associated with rice cultivation under film mulching. Consequently, prospective research directions for the technology of rice film mulching cultivation are outlined, and recommendations for future research into rice cultivation under film mulching are proposed.