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Identification of Differentially Expressed Genes During Anther Abortion of Taigu Genic Male Sterile Wheat by Combining Suppression Subtractive Hybridization and cDNA Array 被引量:1
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作者 Qing-Shan Chang Rong-Hua Zhou +2 位作者 Xiu-Ying Kong Zeng-Liang Yu Ji-Zeng Jia 《Journal of Integrative Plant Biology》 SCIE CAS CSCD 2006年第11期1348-1354,共7页
Talgu Genlc Male Sterile Wheat (TGMSW; Trltlcum aestlvum L.), a dominant genic male sterile germplasm, is of considerable value in the genetic Improvement of wheat because of Its stable Inherence, complete male abor... Talgu Genlc Male Sterile Wheat (TGMSW; Trltlcum aestlvum L.), a dominant genic male sterile germplasm, is of considerable value in the genetic Improvement of wheat because of Its stable Inherence, complete male abortion, and high cross-fertilization rate. To Identify specially transcribed genes In sterile anther, a suppression subtractlve hybridization (aSH) library was constructed with sterile anther as the tester and fertile anther as the driver. A total of 2 304 SSH Inserts amplified by polymerase chain reaction were arrayed using robotic printing. The cDNA arrays were hybridized with 32P-labeled probes prepared from the RNA of forward- and reverse-subtracted anthers. Ninety-six clones were scored as upregulated in sterile anthers compared with the corresponding fertile anthers and some clones were selected for sequencing and analysis In GenBank. Based on their putative functions, 87 non-redundant clones were classified Into the following groups: (i) eight genes Involved In metabolic processes; (11) four material transportation genes; (iii) three signal transductlon-assoclated genes; (iv) four stress response and senescence-associated protein genes; (v) seven other functional protein genes; (vi) five genes with no known function; and (vii) another 56 genes with no match to the databases. To test the hybridization efficiency, eight genes were selected and analyzed by Northern blot. The results of the present study provide a comprehensive overview of the genes and gene products Involved In anther abortion In TGMSW. 展开更多
关键词 ANTHER cDNA array suppression subtractive hybridization Taigu Genic male sterile wheat.
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Structural and Expressional Variation Analyses of Mitochondrial Genomes Reveal Candidate Transcripts for the S^V Cytoplasmic Male Sterility in Wheat(Triticum aestivum L.) 被引量:1
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作者 Junwei Wang Xiaoli Wang +3 位作者 Hong Xu Huiwu Tang Gaisheng Zhang Yao-Guang Liu 《Journal of Genetics and Genomics》 SCIE CAS CSCD 2013年第8期437-439,共3页
Common wheat (Triticum aestivum L.) is one of the most important crops, and intra-specific wheat hybrids have obvious heterosis in yield and protein quality. Therefore, utilization of hybrid wheat varieties offers a... Common wheat (Triticum aestivum L.) is one of the most important crops, and intra-specific wheat hybrids have obvious heterosis in yield and protein quality. Therefore, utilization of hybrid wheat varieties offers an effective way to increase yield and nutrition. Cytoplasmic male sterility (CMS) systems are a useful genetic tool for hybrid crop breeding, and are ideal models for studying the genetic interaction and cooperative function of mitochondrial and nuclear genomes in plants (Schnable and Wise, 1998; Hanson and Bentolila, 2004). 展开更多
关键词 Structural and Expressional Variation Analyses of Mitochondrial Genomes Reveal Candidate Transcripts for the S~V Cytoplasmic male Sterility in wheat Triticum aestivum L CMS gene CS
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