Gibbons have experienced extensive karyotype rearrangements during evolution and represent an ideal model for studying the underlying molecular mechanism of evolutionary chromosomal rearrangements. It is anticipated t...Gibbons have experienced extensive karyotype rearrangements during evolution and represent an ideal model for studying the underlying molecular mechanism of evolutionary chromosomal rearrangements. It is anticipated that the cloning and sequence characterization of evolutionary chromosomal breakpoints will provide vital insights into the molecular force that has driven such a radical karyotype reshuffle in gibbons. We constructed and characterized a high-quality fosmid li- brary of the white-cheeked gibbon （Nomascus leucogenys） containing 192,000 non- redundant clones with an average insert size of 38 kb and 2.5-fold genome coverage. By end sequencing of 100 randomly selected fosmid clones, we generated 196 se- quence tags for the library. These end-sequenced fosmid clones were then mapped onto the chromosomes of the white-cheeked gibbon by fluorescence in situ hy- bridization~ and no spurious chimeric clone was detected. BLAST search against the human genome showed a good correlation between the number of hit clones and the number of chromosomes, an indication of unbiased chromosomal distribu- tion of the fosmid library. The chromosomal distribution of the mapped clones is also consistent with the BLAST search result against human and white-cheeked gibbon genomes. The fosmid library and the mapped clones will serve as a valu- able resource for further studying gibbons＇ chromosomal rearrangements and the underlying molecular mechanism as well as for comparative genomic study in the lesser apes.展开更多
本文报道了云南省勐腊自然保护区内的白颊长臂猿(Hylobat es concolor leucogenys)全年的觅食情况及食谱。长臂猿喜食植物的果,其次分别为叶、嫩枝叶和花,偶食小型动物。植物性食物占93.60％,其中每天采食果、叶、花和嫩枝,叶的时间分...本文报道了云南省勐腊自然保护区内的白颊长臂猿(Hylobat es concolor leucogenys)全年的觅食情况及食谱。长臂猿喜食植物的果,其次分别为叶、嫩枝叶和花,偶食小型动物。植物性食物占93.60％,其中每天采食果、叶、花和嫩枝,叶的时间分别占觅食时间的38.63％、35.52％、4.6％17.29％,动物性食物占6.40％,每天捕食动物性食物的时间占觅食时间的3.89％,主要捕食昆虫。展开更多
基金the National Natural Science Foundation of China（No.30270719 and 30623007）.
文摘Gibbons have experienced extensive karyotype rearrangements during evolution and represent an ideal model for studying the underlying molecular mechanism of evolutionary chromosomal rearrangements. It is anticipated that the cloning and sequence characterization of evolutionary chromosomal breakpoints will provide vital insights into the molecular force that has driven such a radical karyotype reshuffle in gibbons. We constructed and characterized a high-quality fosmid li- brary of the white-cheeked gibbon （Nomascus leucogenys） containing 192,000 non- redundant clones with an average insert size of 38 kb and 2.5-fold genome coverage. By end sequencing of 100 randomly selected fosmid clones, we generated 196 se- quence tags for the library. These end-sequenced fosmid clones were then mapped onto the chromosomes of the white-cheeked gibbon by fluorescence in situ hy- bridization~ and no spurious chimeric clone was detected. BLAST search against the human genome showed a good correlation between the number of hit clones and the number of chromosomes, an indication of unbiased chromosomal distribu- tion of the fosmid library. The chromosomal distribution of the mapped clones is also consistent with the BLAST search result against human and white-cheeked gibbon genomes. The fosmid library and the mapped clones will serve as a valu- able resource for further studying gibbons＇ chromosomal rearrangements and the underlying molecular mechanism as well as for comparative genomic study in the lesser apes.
文摘本文报道了云南省勐腊自然保护区内的白颊长臂猿(Hylobat es concolor leucogenys)全年的觅食情况及食谱。长臂猿喜食植物的果,其次分别为叶、嫩枝叶和花,偶食小型动物。植物性食物占93.60％,其中每天采食果、叶、花和嫩枝,叶的时间分别占觅食时间的38.63％、35.52％、4.6％17.29％,动物性食物占6.40％,每天捕食动物性食物的时间占觅食时间的3.89％,主要捕食昆虫。
