[Objective] This research aimed at exploring an effective way for inoculation and identification of chrysanthemum white rust under controlled conditions. [Method] By combining the observation methods with the naked ey...[Objective] This research aimed at exploring an effective way for inoculation and identification of chrysanthemum white rust under controlled conditions. [Method] By combining the observation methods with the naked eye and under optical microscope, we had established the identification standards for chrysanthemum white rust with six classifications and optimized artificial inoculation methods in vitro. [Result] The results showed that bottled cuttings identification method and petri dished leaves identification method both can be used for identification in vitro of chrysanthemum white rust, bottled cuttings identification method had shown better effects than petri dished leaves identification method, and was supposed to be best artificial inoculation and identification method in vitro. [Conclusion] This research had provided a scientific method for safe and effective researches on chrysanthemum white rust, in order to control the occurrence and diffusion of this quarantine disease.展开更多
Whitebark pine （Pinus albicaulis） populations are in decline across the species＇ range due to historic wildfire exclusion, mountain pine beetle （Dendroctonus ponderosae, MPB） outbreaks, and an invasive fungal pat...Whitebark pine （Pinus albicaulis） populations are in decline across the species＇ range due to historic wildfire exclusion, mountain pine beetle （Dendroctonus ponderosae, MPB） outbreaks, and an invasive fungal pathogen causing the disease white pine blister rust （Cronartium ribicola, WPBR）. Despite reliance on stand- replacing fires, information on whitebark pine regeneration occurrence is limited and the trajectory of future forests is largely unknown in some areas of the range. Regeneration densities were assessed in burned and adjacent non-burned areas at six high elevation locations in northwest Wyoming where stand-replacing fires occurred 8-32 years before thesurveys. In these locations at the eastern extent of the species range, we assessed what site factors were associ- ated with regeneration success. Whitebark pine regen- eration density was greater and seedlings were older in non-burned compared to burned areas. Within burns, north aspects had more regeneration than south aspects. Potential seed source densities and other species＇ regeneration were positively related to whitebark p^ne regeneration densities in burned areas. South facing slopes or grass covered areas may have either delayed or no regeneration of whitebark pine without the help of artificial planting.展开更多
The white rust,caused by Puccinia horiana was one of the most important epidemic diseases on Chrysanthemum,and also was a quarantine action pest in the world.Trials determined the teliospores germinating biology of P....The white rust,caused by Puccinia horiana was one of the most important epidemic diseases on Chrysanthemum,and also was a quarantine action pest in the world.Trials determined the teliospores germinating biology of P.horiana : the teliospores were germinated between 4 ℃ and 32 ℃,while the optimum temperature range was 15 ℃ to 24 ℃,especially between 18 ℃ and 21 ℃.Water was necessary for the teliospores germination.Without free water,it couldn’t germinate in 24 h,even with 100 % R.H.The propriety of pH for germination was pH 4 to 6.5, while pH 6 was the most favorite.2 % glucose solution promoted the germination of the teliospores evidently,whereas the fresh juice of chrysanthemum foliages restrained it prominently.Light didn’t influence on the germination.展开更多
基金Supported by the"Eleventh Five-Year"National Technology Support Program"Breeding of New Varieties of High Yield and Quality of Major Commercial Flowers"(2006BAD01A18)the Postdoctoral Research Fund of Shenyang Agricultural University~~
文摘[Objective] This research aimed at exploring an effective way for inoculation and identification of chrysanthemum white rust under controlled conditions. [Method] By combining the observation methods with the naked eye and under optical microscope, we had established the identification standards for chrysanthemum white rust with six classifications and optimized artificial inoculation methods in vitro. [Result] The results showed that bottled cuttings identification method and petri dished leaves identification method both can be used for identification in vitro of chrysanthemum white rust, bottled cuttings identification method had shown better effects than petri dished leaves identification method, and was supposed to be best artificial inoculation and identification method in vitro. [Conclusion] This research had provided a scientific method for safe and effective researches on chrysanthemum white rust, in order to control the occurrence and diffusion of this quarantine disease.
基金supported by USDA Forest Service,Region 1 and 4,Whitebark Pine Restoration Fund,Agreement No.07-CA-11010000-009the Colorado State University,Agricultural Experiment Station
文摘Whitebark pine （Pinus albicaulis） populations are in decline across the species＇ range due to historic wildfire exclusion, mountain pine beetle （Dendroctonus ponderosae, MPB） outbreaks, and an invasive fungal pathogen causing the disease white pine blister rust （Cronartium ribicola, WPBR）. Despite reliance on stand- replacing fires, information on whitebark pine regeneration occurrence is limited and the trajectory of future forests is largely unknown in some areas of the range. Regeneration densities were assessed in burned and adjacent non-burned areas at six high elevation locations in northwest Wyoming where stand-replacing fires occurred 8-32 years before thesurveys. In these locations at the eastern extent of the species range, we assessed what site factors were associ- ated with regeneration success. Whitebark pine regen- eration density was greater and seedlings were older in non-burned compared to burned areas. Within burns, north aspects had more regeneration than south aspects. Potential seed source densities and other species＇ regeneration were positively related to whitebark p^ne regeneration densities in burned areas. South facing slopes or grass covered areas may have either delayed or no regeneration of whitebark pine without the help of artificial planting.
文摘The white rust,caused by Puccinia horiana was one of the most important epidemic diseases on Chrysanthemum,and also was a quarantine action pest in the world.Trials determined the teliospores germinating biology of P.horiana : the teliospores were germinated between 4 ℃ and 32 ℃,while the optimum temperature range was 15 ℃ to 24 ℃,especially between 18 ℃ and 21 ℃.Water was necessary for the teliospores germination.Without free water,it couldn’t germinate in 24 h,even with 100 % R.H.The propriety of pH for germination was pH 4 to 6.5, while pH 6 was the most favorite.2 % glucose solution promoted the germination of the teliospores evidently,whereas the fresh juice of chrysanthemum foliages restrained it prominently.Light didn’t influence on the germination.
