土壤盐渍化是影响蚕豆生长的重要非生物胁迫之一。蚕豆耐盐碱性种质鉴定为挖掘耐盐碱基因和耐盐碱品种选育奠定基础,对利用盐碱地具有重要意义。本研究对155份国内外蚕豆种质全生育期利用基质+混合盐碱(9 g L^(-1) NaCl+Na_(2)CO_(3)+Na...土壤盐渍化是影响蚕豆生长的重要非生物胁迫之一。蚕豆耐盐碱性种质鉴定为挖掘耐盐碱基因和耐盐碱品种选育奠定基础,对利用盐碱地具有重要意义。本研究对155份国内外蚕豆种质全生育期利用基质+混合盐碱(9 g L^(-1) NaCl+Na_(2)CO_(3)+Na_(2)SO_(4),pH 10.5)进行胁迫处理。测定了成苗率、盐害指数、株高、鲜重、干重、叶绿素含量和氮含量7个指标,采用相关性、主成分、隶属函数和系统聚类分析,对各种质的耐盐碱性进行了综合评价和归类,并采用逐步回归分析建立了耐盐碱性预测回归方程。结果表明,(1)筛选出耐盐碱(20≤盐害指数<40)蚕豆种质3份,占1.94%;中耐盐碱(40≤盐害指数<60)种质8份,占5.16%;未发现高耐盐碱(盐害指数<20)蚕豆种质。(2)盐害指数与株高、鲜重、干重、叶绿素含量和氮含量呈极显著负相关(P<0.01)。(3)鲜重、株高、盐害指数、叶绿素含量和成苗率5个指标可以作为蚕豆全生育期耐盐碱鉴定评价指标。(4)155份蚕豆种质可分为2大类群,其中耐盐碱种质类群具有较高的成苗率、生物量、含氮量和叶绿素含量。研究结果可为蚕豆耐盐碱机理研究、耐盐碱基因挖掘和耐盐碱品种的选育提供可靠的材料。展开更多
盐胁迫是许多沿海地区水稻生产的主要制约因素,尤其是沿海地区的咸淡水交汇区域。耐盐性是一种复杂的性状,可以通过QTL定位来帮助耐盐育种,以培育更高耐盐性的水稻品种。本研究供体亲本为沿海深水稻品种赤禾,受体亲本为美国水稻品种Lemo...盐胁迫是许多沿海地区水稻生产的主要制约因素,尤其是沿海地区的咸淡水交汇区域。耐盐性是一种复杂的性状,可以通过QTL定位来帮助耐盐育种,以培育更高耐盐性的水稻品种。本研究供体亲本为沿海深水稻品种赤禾,受体亲本为美国水稻品种Lemont,杂交获得174份F9代的重组自交系,在芽期、苗期和生殖生长期分别利用浓度为15 g L^(-1)、5 g L^(-1)和5~6 g L^(-1)的NaCl进行胁迫,通过芽期相对发芽率、苗期耐盐性评级和生殖生长期的7个表型性状为基础数据,利用142个SSR分子标记绘制连锁遗传图并进行QTL分析。鉴定结果发现,赤禾在芽期表现敏盐,在苗期和生殖生长期表现耐盐;Lemont相反。3个生长时期分别有70.11%、50.57%和60.34%的品系表现为弱耐盐性,而且耐盐性为弱的负相关。本研究共鉴定出33个LOD值为2.52~10.32的QTL,解释0.06%~13.68%的表型遗传变异,解释最大遗传变异的QTL均由耐盐亲本贡献,其中芽期4个、苗期6个和生殖生长期23个位点,并在生殖生长期发现4个QTL重叠区域。这些QTL可以进一步研究,不仅为提高水稻育种的耐盐性提供了新的遗传资源,还有助于在水稻耐盐育种中,提高水稻品种的耐盐性。展开更多
In order to clarify drought resistance of proso millet applied in field production in Shanxi, we identified its drought resistance during the whole growth period and measured nine traits, including plant height, panic...In order to clarify drought resistance of proso millet applied in field production in Shanxi, we identified its drought resistance during the whole growth period and measured nine traits, including plant height, panicle length, stem diameter, panicle weight, grain weight per spike, straw weight per plant, node numbers of main stem, 1 000-grain weight and effective panicles, under different treatments. Principal component analysis and stepwise regression analysis were used for comprehensive evaluation of drought resistance during the whole growth period and screening the drought index. The results showed that there were correlations among 9 phenotypic traits. The coefficient of variation of panicle weight and straw weight per plant sharply reduced under normal water supply and drought stress. Landrace "yellow millet" had the strongest drought resistance(D =0.87), integrated drought D value and membership function value of yield had significant correlation(r =0.515 9, P〈0.05). Panicle weight and straw weight per plant had significant impact on drought resistance and could be taken as comprehensive indexes of drought resistance identification in growth period.展开更多
文摘土壤盐渍化是影响蚕豆生长的重要非生物胁迫之一。蚕豆耐盐碱性种质鉴定为挖掘耐盐碱基因和耐盐碱品种选育奠定基础,对利用盐碱地具有重要意义。本研究对155份国内外蚕豆种质全生育期利用基质+混合盐碱(9 g L^(-1) NaCl+Na_(2)CO_(3)+Na_(2)SO_(4),pH 10.5)进行胁迫处理。测定了成苗率、盐害指数、株高、鲜重、干重、叶绿素含量和氮含量7个指标,采用相关性、主成分、隶属函数和系统聚类分析,对各种质的耐盐碱性进行了综合评价和归类,并采用逐步回归分析建立了耐盐碱性预测回归方程。结果表明,(1)筛选出耐盐碱(20≤盐害指数<40)蚕豆种质3份,占1.94%;中耐盐碱(40≤盐害指数<60)种质8份,占5.16%;未发现高耐盐碱(盐害指数<20)蚕豆种质。(2)盐害指数与株高、鲜重、干重、叶绿素含量和氮含量呈极显著负相关(P<0.01)。(3)鲜重、株高、盐害指数、叶绿素含量和成苗率5个指标可以作为蚕豆全生育期耐盐碱鉴定评价指标。(4)155份蚕豆种质可分为2大类群,其中耐盐碱种质类群具有较高的成苗率、生物量、含氮量和叶绿素含量。研究结果可为蚕豆耐盐碱机理研究、耐盐碱基因挖掘和耐盐碱品种的选育提供可靠的材料。
文摘盐胁迫是许多沿海地区水稻生产的主要制约因素,尤其是沿海地区的咸淡水交汇区域。耐盐性是一种复杂的性状,可以通过QTL定位来帮助耐盐育种,以培育更高耐盐性的水稻品种。本研究供体亲本为沿海深水稻品种赤禾,受体亲本为美国水稻品种Lemont,杂交获得174份F9代的重组自交系,在芽期、苗期和生殖生长期分别利用浓度为15 g L^(-1)、5 g L^(-1)和5~6 g L^(-1)的NaCl进行胁迫,通过芽期相对发芽率、苗期耐盐性评级和生殖生长期的7个表型性状为基础数据,利用142个SSR分子标记绘制连锁遗传图并进行QTL分析。鉴定结果发现,赤禾在芽期表现敏盐,在苗期和生殖生长期表现耐盐;Lemont相反。3个生长时期分别有70.11%、50.57%和60.34%的品系表现为弱耐盐性,而且耐盐性为弱的负相关。本研究共鉴定出33个LOD值为2.52~10.32的QTL,解释0.06%~13.68%的表型遗传变异,解释最大遗传变异的QTL均由耐盐亲本贡献,其中芽期4个、苗期6个和生殖生长期23个位点,并在生殖生长期发现4个QTL重叠区域。这些QTL可以进一步研究,不仅为提高水稻育种的耐盐性提供了新的遗传资源,还有助于在水稻耐盐育种中,提高水稻品种的耐盐性。
基金Supported by the Earmarked Fund for China Agriculture Research(CARS-07-12.5-A12)Shanxi Agricultural Sciences Program(2013gg20)~~
文摘In order to clarify drought resistance of proso millet applied in field production in Shanxi, we identified its drought resistance during the whole growth period and measured nine traits, including plant height, panicle length, stem diameter, panicle weight, grain weight per spike, straw weight per plant, node numbers of main stem, 1 000-grain weight and effective panicles, under different treatments. Principal component analysis and stepwise regression analysis were used for comprehensive evaluation of drought resistance during the whole growth period and screening the drought index. The results showed that there were correlations among 9 phenotypic traits. The coefficient of variation of panicle weight and straw weight per plant sharply reduced under normal water supply and drought stress. Landrace "yellow millet" had the strongest drought resistance(D =0.87), integrated drought D value and membership function value of yield had significant correlation(r =0.515 9, P〈0.05). Panicle weight and straw weight per plant had significant impact on drought resistance and could be taken as comprehensive indexes of drought resistance identification in growth period.