[Objectives]This study was conducted to analyze the quality of several kinds of wild vegetables in Yongchuan District,Chongqing City.[Methods]Five common edible wild herbs,Plantago asiatica,Taraxacum mongolicum,Pterid...[Objectives]This study was conducted to analyze the quality of several kinds of wild vegetables in Yongchuan District,Chongqing City.[Methods]Five common edible wild herbs,Plantago asiatica,Taraxacum mongolicum,Pteridium aquilinum(L.)Kuhn var.latiusculum(Desv.)Underw.ex Heller,Brasenia schreberi and Houttuynia cordata in Yongchuan District,Chongqing,were tested for nitrate,amino acid,reducing sugar,and vitamin C contents.[Results]The vitamin C content was the highest in B.schreberi,reaching 1056 mg/kg,and the lowest in P.aquilinum var.latiusculum,at only 61 mg/kg.T.mongolicum had the highest reducing sugar(E value)and amino acid contents,at 1.01 and 24.3 mg/kg,respectively,and the values were the lowest in P.asiatica,at 0.15 and 9.1 mg/kg,respectively;and the nitrate contents ranked as H.cordata(2529 mg/kg)﹥B.schreberi(2188 mg/kg)﹥P.aquilinum var.latiusculum(1900 mg/kg)﹥P.asiatica(1097 mg/kg)﹥T.mongolicum(1079 mg/kg),which were at the level of medium to high pollution,but within the scope of the national acceptable daily intake(ADI).The nutritional value of wild vegetables is higher than that of vegetables grown in the field,but some wild vegetables are also toxic,which will have adverse effects on human health.[Conclusions]People need to develop and utilize wild vegetable resources scientifically and rationally according to their own needs.展开更多
The cultivation technology were standardized for improving the yield and quality,and the commodity value and the competitiveness of fruit market,and solving the bottleneck problem—fruit quality and yield that affect ...The cultivation technology were standardized for improving the yield and quality,and the commodity value and the competitiveness of fruit market,and solving the bottleneck problem—fruit quality and yield that affect the industrial development of rare and multi-purpose wild fruit Kadsura coccinea(Lem.)A.C.Smith.Through the investigation of K.coccinea production area and planting area,collection and analysis of fruit samples,it was found that in most planting area,the varieties of K.coccinea were mixed,with great differences in fruit quality and yield,which makes it impossible to realize large-scale planting.In view of these problems,a series of studies have been carried out and the technical regulations for standardized planting of rare and multi-purpose wild fruit K.coccinea were formulated.展开更多
More than 1.9 million new colorectal cancer(CRC)cases and 935000 deaths were estimated to occur worldwide in 2020,representing about one in ten cancer cases and deaths.Overall,colorectal ranks third in incidence,but s...More than 1.9 million new colorectal cancer(CRC)cases and 935000 deaths were estimated to occur worldwide in 2020,representing about one in ten cancer cases and deaths.Overall,colorectal ranks third in incidence,but second in mortality.More than half of the patients are in advanced stages at diagnosis.Treatment options are complex because of the heterogeneity of the patient population,including different molecular subtypes.Treatments have included conventional fluorouracil-based chemotherapy,targeted therapy,immunotherapy,etc.In recent years,with the development of genetic testing technology,more and more targeted drugs have been applied to the treatment of CRC,which has further prolonged the survival of metastatic CRC patients.展开更多
旨在探讨新疆野生荒漠肉苁蓉醇提物(ethanol extracts of wild Cistanche deserticola,EEWCD)调节Th1/Th2免疫反应的特点及初步的作用机制。采用卵清白蛋白(ovalbumin,OVA)为抗原,研究EEWCD对小鼠体液免疫,细胞免疫,细胞因子分泌,树突...旨在探讨新疆野生荒漠肉苁蓉醇提物(ethanol extracts of wild Cistanche deserticola,EEWCD)调节Th1/Th2免疫反应的特点及初步的作用机制。采用卵清白蛋白(ovalbumin,OVA)为抗原,研究EEWCD对小鼠体液免疫,细胞免疫,细胞因子分泌,树突状细胞(dendritic cells,DCs)的成熟和调节性T细胞(regulatory T cell,Treg)等的影响。EEWCD低、中、高剂量(分别记作L、M、H)分别与OVA混合,ICR小鼠随机分为0.9%NaCl、EEWCD、OVA、OVA-EEWCD-L、OVA-EEWCD-M、OVA-EEWCD-H和OVA-铝佐剂免疫组。皮下免疫2次,间隔2周。小鼠免疫后,ELISA法检测抗体滴度及分型,MTT检测脾细胞增殖水平,FACS检测CD4^(+)IL-4、CD4^(+)IFN-γ和CD8^(+)IFN-γ的水平,以及DCs表面分子与Treg表达水平。结果显示,EEWCD提高了OVA特异性IgG抗体2~3倍;在初次免疫后21 d显著促进IgG_(1)和IgG_(2a)的表达水平(P<0.05);显著促进OVA特异性脾细胞增殖以及T/B细胞的活化(P<0.05);显著促进CD4^(+)IL-4、CD4^(+)IFN-γ和CD8^(+)IFN-γ的分泌(P<0.05)。同时,EEWCD也显著促进了DCs的CD40、CD80、CD86和MHCII的表达(P<0.05),下调了Treg的水平(P<0.05)。EEWCD-M为最佳剂量。监测免疫后小鼠行为及体重,小鼠行为没有异常,且同一时间点各组体重之间差异不显著(P>0.05)。综上表明,EEWCD能够促进OVA特异性的Th_(1)/Th_(2)免疫反应,尤其促进Th_(1)型反应,具有良好的免疫调节活性,其作用机制可能为通过激活DCs的成熟,促进IL-4和IFN-γ的分泌,降低Treg的水平调节Th_(1)/Th_(2)免疫反应的动态平衡。展开更多
文摘[Objectives]This study was conducted to analyze the quality of several kinds of wild vegetables in Yongchuan District,Chongqing City.[Methods]Five common edible wild herbs,Plantago asiatica,Taraxacum mongolicum,Pteridium aquilinum(L.)Kuhn var.latiusculum(Desv.)Underw.ex Heller,Brasenia schreberi and Houttuynia cordata in Yongchuan District,Chongqing,were tested for nitrate,amino acid,reducing sugar,and vitamin C contents.[Results]The vitamin C content was the highest in B.schreberi,reaching 1056 mg/kg,and the lowest in P.aquilinum var.latiusculum,at only 61 mg/kg.T.mongolicum had the highest reducing sugar(E value)and amino acid contents,at 1.01 and 24.3 mg/kg,respectively,and the values were the lowest in P.asiatica,at 0.15 and 9.1 mg/kg,respectively;and the nitrate contents ranked as H.cordata(2529 mg/kg)﹥B.schreberi(2188 mg/kg)﹥P.aquilinum var.latiusculum(1900 mg/kg)﹥P.asiatica(1097 mg/kg)﹥T.mongolicum(1079 mg/kg),which were at the level of medium to high pollution,but within the scope of the national acceptable daily intake(ADI).The nutritional value of wild vegetables is higher than that of vegetables grown in the field,but some wild vegetables are also toxic,which will have adverse effects on human health.[Conclusions]People need to develop and utilize wild vegetable resources scientifically and rationally according to their own needs.
基金Key R&D projects in Guangxi Zhuang Autonomous Region(GK AB18221091)Scientific Research and Scientific Planning of Guilin,Guangxi(20160223-1).
文摘The cultivation technology were standardized for improving the yield and quality,and the commodity value and the competitiveness of fruit market,and solving the bottleneck problem—fruit quality and yield that affect the industrial development of rare and multi-purpose wild fruit Kadsura coccinea(Lem.)A.C.Smith.Through the investigation of K.coccinea production area and planting area,collection and analysis of fruit samples,it was found that in most planting area,the varieties of K.coccinea were mixed,with great differences in fruit quality and yield,which makes it impossible to realize large-scale planting.In view of these problems,a series of studies have been carried out and the technical regulations for standardized planting of rare and multi-purpose wild fruit K.coccinea were formulated.
文摘More than 1.9 million new colorectal cancer(CRC)cases and 935000 deaths were estimated to occur worldwide in 2020,representing about one in ten cancer cases and deaths.Overall,colorectal ranks third in incidence,but second in mortality.More than half of the patients are in advanced stages at diagnosis.Treatment options are complex because of the heterogeneity of the patient population,including different molecular subtypes.Treatments have included conventional fluorouracil-based chemotherapy,targeted therapy,immunotherapy,etc.In recent years,with the development of genetic testing technology,more and more targeted drugs have been applied to the treatment of CRC,which has further prolonged the survival of metastatic CRC patients.
文摘旨在探讨新疆野生荒漠肉苁蓉醇提物(ethanol extracts of wild Cistanche deserticola,EEWCD)调节Th1/Th2免疫反应的特点及初步的作用机制。采用卵清白蛋白(ovalbumin,OVA)为抗原,研究EEWCD对小鼠体液免疫,细胞免疫,细胞因子分泌,树突状细胞(dendritic cells,DCs)的成熟和调节性T细胞(regulatory T cell,Treg)等的影响。EEWCD低、中、高剂量(分别记作L、M、H)分别与OVA混合,ICR小鼠随机分为0.9%NaCl、EEWCD、OVA、OVA-EEWCD-L、OVA-EEWCD-M、OVA-EEWCD-H和OVA-铝佐剂免疫组。皮下免疫2次,间隔2周。小鼠免疫后,ELISA法检测抗体滴度及分型,MTT检测脾细胞增殖水平,FACS检测CD4^(+)IL-4、CD4^(+)IFN-γ和CD8^(+)IFN-γ的水平,以及DCs表面分子与Treg表达水平。结果显示,EEWCD提高了OVA特异性IgG抗体2~3倍;在初次免疫后21 d显著促进IgG_(1)和IgG_(2a)的表达水平(P<0.05);显著促进OVA特异性脾细胞增殖以及T/B细胞的活化(P<0.05);显著促进CD4^(+)IL-4、CD4^(+)IFN-γ和CD8^(+)IFN-γ的分泌(P<0.05)。同时,EEWCD也显著促进了DCs的CD40、CD80、CD86和MHCII的表达(P<0.05),下调了Treg的水平(P<0.05)。EEWCD-M为最佳剂量。监测免疫后小鼠行为及体重,小鼠行为没有异常,且同一时间点各组体重之间差异不显著(P>0.05)。综上表明,EEWCD能够促进OVA特异性的Th_(1)/Th_(2)免疫反应,尤其促进Th_(1)型反应,具有良好的免疫调节活性,其作用机制可能为通过激活DCs的成熟,促进IL-4和IFN-γ的分泌,降低Treg的水平调节Th_(1)/Th_(2)免疫反应的动态平衡。
