Understanding the diet of threatened wildlife is vital for species-specific conservation and habitat management measures.The Red-crowned Crane(Grus japonensis)is a vulnerable bird distributed in Northeast Asia.Previou...Understanding the diet of threatened wildlife is vital for species-specific conservation and habitat management measures.The Red-crowned Crane(Grus japonensis)is a vulnerable bird distributed in Northeast Asia.Previous dietary studies of this bird focused mainly on its plant food composition based on field observations and microhistological identification.Herein,a total of 45 fecal samples were collected in November,December and January(15 fecal samples each month)from wintering cranes,and then subjected to a high throughput sequencing meta-barcoding approach to determine the primary plant(rbcL)and animal(COI)food items in their diet.A total of 230 operational taxonomic units(OTUs)of plant foods and 371 OTUs of animal foods were obtained.The main plant foods in the wintering period were Miscanthus,Zea,and Hordeum genera,which were similar to those in the breeding and the migration periods.Both agricultural and natural plants were detected,indicating a relatively broad dietary niche for this crane species.However,the main animal foods were representatives of Theridiidae,Megascolecidae,and Agelenidae,in sharp contrast to previous studies.The higher number of small terrestrial arthropods in animal foods might be due to the indirect intake of plants.The composition of both plant and animal foods in the diet showed the highest diversity in December,while it was homogeneous in January.The plant of Zea genus became the main source of nutrition in late winter,as supplementary feeding was performed in the reserve,which could help Red-crowned Cranes to get through the cold season.The results obtained in this work would contribute to the development of effective conservation strategies for the Red-crowned Crane.展开更多
This paper studied the winter habitat selection of red-crowned cranes (Grus japonensis) in Yancheng National Natural Reserve, Jiangsu Province. There were six types of habitat used by red-crowned cranes, i.e. salt-wor...This paper studied the winter habitat selection of red-crowned cranes (Grus japonensis) in Yancheng National Natural Reserve, Jiangsu Province. There were six types of habitat used by red-crowned cranes, i.e. salt-works, aquiculture ponds, reed lands, grassy tidal lands, salting wormwood lands and wheat fields. The wheat field was a new type of habitat used by red-crowned cranes. More than 70 percent of red-crowned cranes chose grassy tidal lands, salting wormwood lands, and reed lands as their most important habitats. In recent years, the distribution of red-crowned cranes moved southward gradually. Red-crowned cranes prefer artificial or semiartificial wetlands rather than original wetlands, successive distribution was broken into fragments.展开更多
Coordination of the aerial servey, the author carried ground census on the population size of Red-crowned crane(Grus japonensis) in Khanka Lakes District from 1984 to 1986. According to the census in spring of 1984, t...Coordination of the aerial servey, the author carried ground census on the population size of Red-crowned crane(Grus japonensis) in Khanka Lakes District from 1984 to 1986. According to the census in spring of 1984, the total number of Red-crowned crane is 72 individuals and 20 nests. So that this ground census has recorded 59 individuals more than the aerial survey of 13 individuals, and the percentage of nesting individuals in this flock is 55.6%, which is obviously higher than 36.9% of the average ratio in China. After this, in fall of 1984 and in spring of 1985 and 1986, we repeated the same censuses three times in the same districts, the results indicated that the flock of the crane in Khanka Lakes District is stable basically, but some individuals interchange with opposite flock of USSR because of gcgraphical adjoin.So, we believe this flock in China and that flock in USSR belong to the same population and suggest two countries should act in concert to studing on this population in future. Meanwhile, the aerial servey cooperate with ground census, it is the best way for reducing missing crane and achieving to high precision of census. From this, total number of Red-crowned crane in China is 542 individuals (483+59 ind ividuals).展开更多
Sex determining gene primers of Oriental White Stork were used to amplify sex-linked gene of the Red-crowned Crane′s W chromosome-specific by PCR for sex identification. The sexes of 7 couples of grown Red-crowned Cr...Sex determining gene primers of Oriental White Stork were used to amplify sex-linked gene of the Red-crowned Crane′s W chromosome-specific by PCR for sex identification. The sexes of 7 couples of grown Red-crowned Cranes and 15 youngs were identified. Through DNA sequence analysis, the identity is 94.77% between Red-crowned Crane and Oriental White Stork. The results of this study suggest that the application of the polymerase chain reaction technique is practicable for determining sex in the Red-crowned Crane.展开更多
Nest-site selection patterns of Red-crowned cranes（Grus japonensis） and the effects of environmental variables were studied during the years of 2002-2008 in Zhalong Nature Reserve,Qiqihar city,northeast China.The ne...Nest-site selection patterns of Red-crowned cranes（Grus japonensis） and the effects of environmental variables were studied during the years of 2002-2008 in Zhalong Nature Reserve,Qiqihar city,northeast China.The nest-site selection pattern of Red-crowned cranes included two orders and three choices：the choice of nest-site habitat type within the macro-habitat order,nest zone selection and nest-site micro-habitat selection within the micro-habitat order.Various habitats（such as Carex swamps and reed fire districts） can be selected as the nest sites for Red-crowned cranes,of which reed swamps（93.15%） are given a preference.Factor Analysis reveals that the micro-habitat selection are affected by four main factors：fire,security（concealment /disturbance）,incubation（conditions,nest-material）,and food.Further analysis reveals that Red-crowned cranes have certain adaptability to the changes of nesting habitat quality in the Zhalong wetlands.In conclusion,fire,reeds,and water were the most important variables for nest-site habitat selection of Red-crowned Cranes in Zhalong Nature Reserve.展开更多
Incorporating private and working lands into protected area networks could mitigate the isolation state of protected areas(PAs) and improve the efficiency of conservation.But how to select patches of land for conserva...Incorporating private and working lands into protected area networks could mitigate the isolation state of protected areas(PAs) and improve the efficiency of conservation.But how to select patches of land for conservation is still a troublesome issue.In this study, the MaxEnt model and irreplaceability index were applied to guide marsh conservation in the Nenjiang River Basin, Northeast China.According to the high accuracy of the MaxEnt model predictions(i.e., the average AUC value = 0.933), the Wuyuer River and Zhalong marshes in the downstream reaches of Wuyuer River are the optimal habitat for the Red-crowned crane and migratory waterfowls.There are 22 marsh patches selected by the patch irreplaceability index for conservation, of which 12 patches had been included in the current network of protected areas.The other 10 patches of marsh(amounting to 1096 km^2) far from human disturbances with high NDVI(up to 0.8) and close distance to water(less than 100 m), which are excluded from the existing network of PAs, should be implemented conservation easement programs to improve the protection efficiency of conservation.Specifically, the marshes at Taha, Tangchi, and Lamadian should be given priority for conservation and restoration to reintroduce migratory waterfowls, as this would lessen the current isolation state of the Zhalong National Nature Reserve.展开更多
基金supported by the National Natural Science Foundation of China(No.31800453)the Biodiversity Investigation,Observation and Assessment Program(2019-2023)+1 种基金the Ministry of Ecology and Environment of China(2110404)the Science and Technology Department of Sichuan Province(No.2022YFS0487).
文摘Understanding the diet of threatened wildlife is vital for species-specific conservation and habitat management measures.The Red-crowned Crane(Grus japonensis)is a vulnerable bird distributed in Northeast Asia.Previous dietary studies of this bird focused mainly on its plant food composition based on field observations and microhistological identification.Herein,a total of 45 fecal samples were collected in November,December and January(15 fecal samples each month)from wintering cranes,and then subjected to a high throughput sequencing meta-barcoding approach to determine the primary plant(rbcL)and animal(COI)food items in their diet.A total of 230 operational taxonomic units(OTUs)of plant foods and 371 OTUs of animal foods were obtained.The main plant foods in the wintering period were Miscanthus,Zea,and Hordeum genera,which were similar to those in the breeding and the migration periods.Both agricultural and natural plants were detected,indicating a relatively broad dietary niche for this crane species.However,the main animal foods were representatives of Theridiidae,Megascolecidae,and Agelenidae,in sharp contrast to previous studies.The higher number of small terrestrial arthropods in animal foods might be due to the indirect intake of plants.The composition of both plant and animal foods in the diet showed the highest diversity in December,while it was homogeneous in January.The plant of Zea genus became the main source of nutrition in late winter,as supplementary feeding was performed in the reserve,which could help Red-crowned Cranes to get through the cold season.The results obtained in this work would contribute to the development of effective conservation strategies for the Red-crowned Crane.
文摘This paper studied the winter habitat selection of red-crowned cranes (Grus japonensis) in Yancheng National Natural Reserve, Jiangsu Province. There were six types of habitat used by red-crowned cranes, i.e. salt-works, aquiculture ponds, reed lands, grassy tidal lands, salting wormwood lands and wheat fields. The wheat field was a new type of habitat used by red-crowned cranes. More than 70 percent of red-crowned cranes chose grassy tidal lands, salting wormwood lands, and reed lands as their most important habitats. In recent years, the distribution of red-crowned cranes moved southward gradually. Red-crowned cranes prefer artificial or semiartificial wetlands rather than original wetlands, successive distribution was broken into fragments.
文摘Coordination of the aerial servey, the author carried ground census on the population size of Red-crowned crane(Grus japonensis) in Khanka Lakes District from 1984 to 1986. According to the census in spring of 1984, the total number of Red-crowned crane is 72 individuals and 20 nests. So that this ground census has recorded 59 individuals more than the aerial survey of 13 individuals, and the percentage of nesting individuals in this flock is 55.6%, which is obviously higher than 36.9% of the average ratio in China. After this, in fall of 1984 and in spring of 1985 and 1986, we repeated the same censuses three times in the same districts, the results indicated that the flock of the crane in Khanka Lakes District is stable basically, but some individuals interchange with opposite flock of USSR because of gcgraphical adjoin.So, we believe this flock in China and that flock in USSR belong to the same population and suggest two countries should act in concert to studing on this population in future. Meanwhile, the aerial servey cooperate with ground census, it is the best way for reducing missing crane and achieving to high precision of census. From this, total number of Red-crowned crane in China is 542 individuals (483+59 ind ividuals).
文摘Sex determining gene primers of Oriental White Stork were used to amplify sex-linked gene of the Red-crowned Crane′s W chromosome-specific by PCR for sex identification. The sexes of 7 couples of grown Red-crowned Cranes and 15 youngs were identified. Through DNA sequence analysis, the identity is 94.77% between Red-crowned Crane and Oriental White Stork. The results of this study suggest that the application of the polymerase chain reaction technique is practicable for determining sex in the Red-crowned Crane.
基金supported by the 11th Five-Year National Science and Technology plans to support key project (No. 2008BADB0B01)the Program for New Century Excellent Talents in Universities, the National Natural Science Foundation of China (No. 30670350 and 31070345)
文摘Nest-site selection patterns of Red-crowned cranes（Grus japonensis） and the effects of environmental variables were studied during the years of 2002-2008 in Zhalong Nature Reserve,Qiqihar city,northeast China.The nest-site selection pattern of Red-crowned cranes included two orders and three choices：the choice of nest-site habitat type within the macro-habitat order,nest zone selection and nest-site micro-habitat selection within the micro-habitat order.Various habitats（such as Carex swamps and reed fire districts） can be selected as the nest sites for Red-crowned cranes,of which reed swamps（93.15%） are given a preference.Factor Analysis reveals that the micro-habitat selection are affected by four main factors：fire,security（concealment /disturbance）,incubation（conditions,nest-material）,and food.Further analysis reveals that Red-crowned cranes have certain adaptability to the changes of nesting habitat quality in the Zhalong wetlands.In conclusion,fire,reeds,and water were the most important variables for nest-site habitat selection of Red-crowned Cranes in Zhalong Nature Reserve.
基金Under the auspices of National Key Research and Development Program of China(No.2016YFA0600401)the Key Research Program of Frontier Sciences from Chinese Academy of Sciences+1 种基金Fundamental Research Funds in Heilongjiang Provincial Universities(No.135209252,135309359)the Philosophy and Social Sciences Research Plan of Heilongjiang Province(No.16JLC01)
文摘Incorporating private and working lands into protected area networks could mitigate the isolation state of protected areas(PAs) and improve the efficiency of conservation.But how to select patches of land for conservation is still a troublesome issue.In this study, the MaxEnt model and irreplaceability index were applied to guide marsh conservation in the Nenjiang River Basin, Northeast China.According to the high accuracy of the MaxEnt model predictions(i.e., the average AUC value = 0.933), the Wuyuer River and Zhalong marshes in the downstream reaches of Wuyuer River are the optimal habitat for the Red-crowned crane and migratory waterfowls.There are 22 marsh patches selected by the patch irreplaceability index for conservation, of which 12 patches had been included in the current network of protected areas.The other 10 patches of marsh(amounting to 1096 km^2) far from human disturbances with high NDVI(up to 0.8) and close distance to water(less than 100 m), which are excluded from the existing network of PAs, should be implemented conservation easement programs to improve the protection efficiency of conservation.Specifically, the marshes at Taha, Tangchi, and Lamadian should be given priority for conservation and restoration to reintroduce migratory waterfowls, as this would lessen the current isolation state of the Zhalong National Nature Reserve.
