Ninety wild strains of Agaricus collected in China,including 44 strains preliminarily identified as Agaricus bisporus by isozyme electrophoresis,were studied using SRAP and ISSR techniques.Eighteen specific SRAP bands...Ninety wild strains of Agaricus collected in China,including 44 strains preliminarily identified as Agaricus bisporus by isozyme electrophoresis,were studied using SRAP and ISSR techniques.Eighteen specific SRAP bands and 12 specific ISSR bands were scored,and a cluster dendrogram was constructed.The 90 wild strains of Agaricus were separated into two major groups,the first consisting of wild strains of A.bisporus and the second consisting of other Agaricus strains.Forty-one wild strains of A.bisporus collected in Sichuan and Tibet formed four groups according to the geographical location of the collection area.White A.bisporus strains from Xinjiang and Tibet(Xinjiang Wild,AgX04 and AgX042)exhibited unique band patterns resulting in lower similarity coefficient values(10%～13%)when compared with brown or light-brown A.bisporus strains.展开更多
[Objectives] To study the effect of different culture conditions on the growth of wild I. cicadae Miq. liquid strain. [Methods]The I. cicadae Miq. strain was inoculated into the liquid culture medium with different ca...[Objectives] To study the effect of different culture conditions on the growth of wild I. cicadae Miq. liquid strain. [Methods]The I. cicadae Miq. strain was inoculated into the liquid culture medium with different carbon sources,nitrogen sources,micronutrients and p H,cultured in the constant temperature shaking incubator with rotating speed of 120 r/min at 19℃,and the mycelium pellet diameter,density and weight were compared between different treatments. [Results] The results showed that the optimum components of I. cicadae Miq.strain liquid included soluble starch,milk powder and vitamin B_(12),and the optimum p H was 5. 0-6. 0. [Conclusions] Soluble starch was the most suitable carbon source for the culture medium of I. cicadae Miq. liquid strain; milk powder was the most suitable nitrogen source for the culture medium of I. cicadae Miq. liquid strain; the most suitable p H was 5. 0-9. 0 for the mycelial growth of I. cicadae Miq.; the formula and mixture ratio of the optimum culture medium for the growth of liquid strain were determined.展开更多
The autolysis and proteolysis are important features in the strains of L. lactis used in the manufacture of cheese. The autolytic and proteolytic activity of L. lactis has been linked with the development of flavor an...The autolysis and proteolysis are important features in the strains of L. lactis used in the manufacture of cheese. The autolytic and proteolytic activity of L. lactis has been linked with the development of flavor and texture in the cheese. On the other hand, there is a growing interest in new strains isolated from raw-milk cheeses and vegetables. These wild-strains have showed different features of industrial importance in comparison with those observed in commercial cultures. However, it still not clear if the autolytic and proteolytic properties of these wild-strains differ from the industrial strains. The objective of this work was to assess the autolytic and proteolytic activities of 21 strains of L. lactis isolated from diverse sources. The rates of autolysis and proteolysis observed in vitro were highly strain-dependent. The pH and the NaCl concentration in the media affected significantly the autolysis of L. lactis. The strains isolated from vegetable showed in general low and medium autolytic activity, whereas the strains isolated from raw-milk cheeses had medium to high autolytic activity. The strain with highest proteolytic activity was a strain isolated from corn leaves. Although still not clear how this strain acquired this pronounced characteristic.展开更多
基金Supported by National Fund of Natural Sciences(No.30671456,30771503)Key Science & Technology Programsof Fujian Province(No.2006S1001)+1 种基金Fujian Fund of Natural Sciences(No.B0620004)Key S&T InnovationItems of Fujian Academy of Agricultural Sciences(2007)
文摘Ninety wild strains of Agaricus collected in China,including 44 strains preliminarily identified as Agaricus bisporus by isozyme electrophoresis,were studied using SRAP and ISSR techniques.Eighteen specific SRAP bands and 12 specific ISSR bands were scored,and a cluster dendrogram was constructed.The 90 wild strains of Agaricus were separated into two major groups,the first consisting of wild strains of A.bisporus and the second consisting of other Agaricus strains.Forty-one wild strains of A.bisporus collected in Sichuan and Tibet formed four groups according to the geographical location of the collection area.White A.bisporus strains from Xinjiang and Tibet(Xinjiang Wild,AgX04 and AgX042)exhibited unique band patterns resulting in lower similarity coefficient values(10%～13%)when compared with brown or light-brown A.bisporus strains.
基金Supported by"Six Talents Peaks"Project in Jiangsu Province in 2015(NY-024)Project of Jiangsu Polytechnic College of Agriculture and Forestry(2014KJ28)
文摘[Objectives] To study the effect of different culture conditions on the growth of wild I. cicadae Miq. liquid strain. [Methods]The I. cicadae Miq. strain was inoculated into the liquid culture medium with different carbon sources,nitrogen sources,micronutrients and p H,cultured in the constant temperature shaking incubator with rotating speed of 120 r/min at 19℃,and the mycelium pellet diameter,density and weight were compared between different treatments. [Results] The results showed that the optimum components of I. cicadae Miq.strain liquid included soluble starch,milk powder and vitamin B_(12),and the optimum p H was 5. 0-6. 0. [Conclusions] Soluble starch was the most suitable carbon source for the culture medium of I. cicadae Miq. liquid strain; milk powder was the most suitable nitrogen source for the culture medium of I. cicadae Miq. liquid strain; the most suitable p H was 5. 0-9. 0 for the mycelial growth of I. cicadae Miq.; the formula and mixture ratio of the optimum culture medium for the growth of liquid strain were determined.
文摘The autolysis and proteolysis are important features in the strains of L. lactis used in the manufacture of cheese. The autolytic and proteolytic activity of L. lactis has been linked with the development of flavor and texture in the cheese. On the other hand, there is a growing interest in new strains isolated from raw-milk cheeses and vegetables. These wild-strains have showed different features of industrial importance in comparison with those observed in commercial cultures. However, it still not clear if the autolytic and proteolytic properties of these wild-strains differ from the industrial strains. The objective of this work was to assess the autolytic and proteolytic activities of 21 strains of L. lactis isolated from diverse sources. The rates of autolysis and proteolysis observed in vitro were highly strain-dependent. The pH and the NaCl concentration in the media affected significantly the autolysis of L. lactis. The strains isolated from vegetable showed in general low and medium autolytic activity, whereas the strains isolated from raw-milk cheeses had medium to high autolytic activity. The strain with highest proteolytic activity was a strain isolated from corn leaves. Although still not clear how this strain acquired this pronounced characteristic.
