Hypoxia-mediated programmed cell death is involved in the formation of wooden breast in broilers

Background Wooden breast(WB)myopathy is a common myopathy found in commercial broiler chickens worldwide.Histological examination has revealed that WB myopathy is accompanied by damage to the pectoralis major(PM)muscle.However,the underlying mechanisms responsible for the formation of WB in broilers have not been fully elucidated.This study aimed to investigate the potential role of hypoxia-mediated programmed cell death(PCD)in the formation of WB myopathy.Results Histological examination and biochemical analysis were performed on the PM muscle of the control(CON)and WB groups.A significantly increased thickness of the breast muscle in the top,middle,and bottom portions(P<0.01)was found along with pathological structure damage of myofibers in the WB group.The number of capillaries per fiber in PM muscle,and the levels of p O_(2) and s O_(2) in the blood,were significantly decreased(P<0.01),while the levels of p CO_(2) and TCO_(2) in the blood were significantly increased(P<0.05),suggesting hypoxic conditions in the PM muscle of the WB group.We further evaluated the PCD-related pathways including autophagy,apoptosis,and necroptosis to understand the consequence response to enhanced hypoxic conditions in the PM muscle of birds with WB.The ratio of LC3 II to LC3 I,and the autophagy-related factors HIF-1α,BNIP3,Beclin1,AMPKα,and ULK1 at the m RNA and protein levels,were all significantly upregulated(P<0.05),showing that autophagy occurred in the PM muscle of the WB group.The apoptotic index,as well as the expressions of Bax,Cytc,caspase 9,and caspase 3,were significantly increased(P<0.05),whereas Bcl-2 was significantly decreased(P<0.05)in the WB-affected PM muscle,indicating the occurrence of apoptosis mediated by the mitochondrial pathway.Additionally,the expressions of necroptosis-related factors RIP1,RIP3,and MLKL,as well as NF-κB and the pro-inflammatory cytokines TNF-α,IL-1β,and IL-6,were all significantly enhanced(P<0.05)in the WB-affected PM muscle.Conclusions The WB myopathy reduces blood supply and induces hypoxia in the PM muscle,which is closely related to the occurrence of PCD including apoptosis,autophagy,and necroptosis within myofibers,and finally leads to abnormal muscle damage and the development of WB in broilers.

Joinery and Structure:Cultural and Technical Comparison between Traditional Wooden Framing Systems in East Asia and Western Europe

Traditional wooden joinery is the craft of connecting wood pieces for buildings or producing utensils by utilizing only the geometry of the components and material properties of the wood,without other materials such as glue or metal connectors.In its thousands of years of tradition,this construction technique has achieved high accomplishment in both East Asian and European architectural civilizations.Although sharing the same basic principles,joinery techniques vary between regions and cultures,rooted in the geographical environment,available wood species and their material properties,characteristics of craftsmanship,tools,and structural systems.Wood framing systems from China,Japan,and Western Europe are selected for comparison to assess the relationship between wood joinery and other aspects of building technology.The main conclusions include:in East Asia,the building tradition pays great attention to the design and execution of joinery(sunmao榫卯),making it responsible for multiple functions including architectural mechanics and the stability of the entire frame,which leads to a broader role meant by the term“joint”itself,while in Europe joinery is treated as the“node”or literally“joint”of the structure.Although in both East Asia and Europe wooden joinery serves as an aesthetic factor of the structure,its expression in East Asia is subtle,veiled,and restrained,while in Europe it is explicit and direct.The most important lesson we learn from the study of traditional joinery technology is that it should be seen in the context of the building process as a whole,taking into account geographical environment,material resources,craftsmanship,tools,construction methods,structural form,and the structural system.

The Research on Wooden Arched Bridges and Traditional Villages' Environmental Image in East Fujian Province of China——A Case Study of Youxi Ancient Village in Shouning County of East Fujian

By taking Youxi Village which was the typical village with wooden arched bridges in East Fujian for example, it had discussed landforms, geology, soil, climate and vegetation in natural environment which could be perceived and village's cultural and historical background. Based on relevant research results in academic field, it had studied the relationship between wooden arched bridges and villages' environment in East Fujian, and further analyzed the relation of wooden arched bridges as traditional architectural heritages with village's living condition and history. On this basis, it had analyzed imaginability of natural environment of Youxi Village from the aspects of village's site and stream's trend, and imaginability of cultural environment by taking Wenming Bridge, Liren Bridge and Zhangkeng Bridge for example. It was considered that wooden arched bridge was the consolidation of natural environment and had been the recognizable image at villages of East Fujian, manifesting heavy cultural characteristic.

Transformation of Traditional Wooden Houses in Rural Areas of Southwest China

By analyzing the status quo of traditional wooden houses in southwest China,it was proposed that although traditional wooden houses have many deficiencies in overall layout,type of flat,structure design,thermal protection and insulation,they are popular in southwest China due to particular resources and environment of the local areas,and advantages of wooden houses.It was stressed that the antiseismic structure of traditional wooden houses should be borrowed and modern materials applied to make residential buildings in rural areas more beautiful,comfortable,stable,energy-saving and environment-friendly.Problems of traditional wooden houses in planning,type of flat,structure design,thermal protection and insulation were analyzed,corresponding improvement measures were given,and advantages of improved wooden houses were concluded as "simple and quick construction;concise and beautiful style,favorable flat type;low-carbon and environment-friendly;high antiseismic capacity;low requirements on building materials,reusable materials".

Modal parameter identification and damping ratio estimation from the full-scale measurements of a typical Tibetan wooden structure

Tibetan heritage buildings have a high historical and cultural value. They have endured adverse environmental loadings over hundreds of years without significant damage. However, there are few reports on their structural characteristics under normal environmental loadings and their behavior under dynamic loadings. In this research, a typical Tibetan wooden wall-frame building is selected to study its dynamic characteristics. Field measurements of the structure were conducted under environmental excitation to collect acceleration responses. The stochastic subspace identification （SSI） method was adopted to calculate the structural modal parameters and obtain the out-of-plane vibration characteristics of the slab and frames. The results indicated that the wall-frame structure had a lower out-of-plane stiffness and greater in-plane stiffness due to the presence of stone walls. Due to poor identified damping ratio estimates from the SSI method, a method based on the variance upper bound was proposed to complement the existing variance lower bound method for estimating the modal damping ratio to address the significant damping variability obtained from different points and measurements. The feasibility of the proposed method was illustrated with the measured data from the floor slab of the structure. The variance lower and upper bound methods both provided consistent results compared to those from the traditional SSI method.

Improvement of thermal performance of envelopes for traditional wooden vernacular dwellings of Tujia Minority in Western Hunan,China

Thermal performance of envelopes and indoor thermal environment were technologically improved for traditional wooden vernacular dwellings of Tujia Minority in Western Hunan, China, on the premise of protecting their conventional styles. Thermal insulation boards and wooden boards were added to the interior side of external walls of vernacular dwellings to form two layers of air cavities, so as to gain excellent thermal performance. The indoor temperature of such dwellings after reconstruction was apparently improved compared with the data before reconstruction both in winter and summer, which verified the feasibility and the effectiveness of the reconstruction technologies proposed.

The Effect of Soaking with Wooden Ash and Malting upon Some Nutritional Properties of Sorghum Flour Used for Impeke,a Traditional Burundian Malt-Based Sorghum Beverage

The objective of the present work was to determine the effect of wooden ash extract on anti-nutritional factors and to assess the effect of soaking with malting on nutritional properties of sorghum flour used for impeke. The addition of wooden ash extract during 24 h of soaking resulted in significant decrease in tannin by 50.2% and the decrease was observed to be progressive as malting time increases. 5 d of malting resulted in significant decrease in tannin by 69.3% and in phytic acid by 66.4% with slight decrease in ash, lipid, fiber, and starch. Malting showed an increased percentage of protein, essential amino acids, and then in vitro protein digestibility were markedly improved with increasing malting time. Sugars analysis proved a significant increase in maltose, glucose, fructose, and structural analysis of sorghum starch displayed porosity on granule＇s surface susceptible to the amylolysis.

A feasibility study of using two-component polyurethane adhesive in constructing wooden structures

This investigation was conducted to determine the feasibility of using a two-component polyurethane （PUR） adhesive, with special waterproof properties, in constructing wooden structures. We designed and conducted tests to compare the shear strength and adhesion performance of PUR with polyvinyl acetate （PVAc） adhesive on block-shear specimens constructed of oriental beech （Fagus orientalis L.）, fir （Abies alba Mill.）, poplar （Populus deltoides Bartr.）, white oak （Quercus alba L.）, sycamore （Platanus orientalis L.） and white walnut （Juglans cinerea L.）. The values of the percentage of wood failure were also determined in specimens constructed with each adhesive. The highest shear strength values of both adhesives were obtained in specimens constructed of beech, while the lowest shear strength values were obtained in fir and poplar specimens. Average shear strength of the PUR adhesive was 16.5% higher than that of the PVAc adhesive. Specimens constructed of fir, poplar and sycamore were characterised by the highest percentages of wood failure, whereas the lowest average percentages of wood failure were obtained in beech and oak specimens. With the exception of oak specimens, there was no statistically significant difference between percentage of wood failure among the PUR and PVAc adhesives. Generally, the PUR adhesive showed an acceptable adhesion performance on wood materials used in our study.

Dominant changes in the breast muscle lipid profiles of broiler chickens with wooden breast syndrome revealed by lipidomics analyses

Background: Chicken is the most consumed meat worldwide and the industry has been facing challenging myopathies. Wooden breast(WB), which is often accompanied by white striping(WS), is a serious myopathy adversely affecting meat quality of breast muscles. The underlying lipid metabolic mechanism of WB affected broilers is not fully understood.Results: A total of 150 chickens of a white-feathered, fast-growing pure line were raised and used for the selection of WB, WB + WS and control chickens. The lipids of the breast muscle, liver, and serum from different chickens were extracted and measured using ultra performance liquid chromatography(UPLC) plus Q-Exactive Orbitrap tandem mass spectrometry. In the breast, 560 lipid molecules were identified. Compared to controls, 225/225 of 560 lipid molecules(40.2%) were identified with differential abundance(DA), including 92/100 significantly increased neutral lipids and 107/98 decreased phospholipids in the WB/WB + WS groups, respectively. The content of monounsaturated fatty acids(MUFA) was significantly higher, and the polyunsaturated fatty acids(PUFA) and saturated fatty acids(SFA) were significantly lower in the affected breasts. In the liver, 434 lipid molecules were identified, and 39/61 DA lipid molecules(6.7%/14.1%) were detected in the WB and WB + WS groups, respectively. In the serum, a total of 529 lipid molecules were identified and 4/44 DA lipid molecules(0.8%/8.3%) were detected in WB and WB + WS group, respectively. Compared to controls, the content of MUFAs in the serum and breast of the WB + WS group were both significantly increased, and the content of SFAs in two tissues were both significantly decreased. Only five lipid molecules were consistently increased in both liver and serum in WB + WS group.Conclusions: We have found for the first time that the dominant lipid profile alterations occurred in the affected breast muscle. The relative abundance of 40.2% of lipid molecules were changed and is characteristic of increased neutral lipids and decreased phospholipids in the affected breasts. Minor changes of lipid profiles in the liver and serum of the affected groups were founded. Comprehensive analysis of body lipid metabolism indicated that the abnormal lipid profile of WB breast may be independent of the liver metabolism.

Research on Technology of Wooden Furniture CAD System

This paper introduces the current situation of domestic furniture production as well as world furniture CAD products, puts forward the necessity of developing a dedicated CADsystem——WFCAD and its main functions. It analyzes in detail the applied technology and keytechniques, including geometric modeling, assembly, joint design, database linking and management. The application of WFCAD in furniture manufacturing enterprises and its further improvement are outlined.

Intraorbital wooden foreign bodies: case series and literature review

Intraorbital wooden foreign bodies(IOWFBs) constitute a relatively rare ocular trauma, which occupy a special type of intraorbital foreign bodies(IOFBs). Data regarding IOWFBs must be obtained from case reports or small case series due to their rarity. Here, we reported 5 cases of IOWFBs and reviewed the related literatures, which could provide comprehensive information regarding the clinical manifestations, diagnosis, and surgical treatment of IOWFBs. Combined with the published literature, a total of 51 independent cases were counted after we added 5 cases. Among them, the number of male and female patients was 35 and 16 respectively;the mean age was 27.3±18.2(range 1-66)y. Obviously, the disorder seemed to occur mainly in young and middle-aged people. Because of the diversity in the clinical manifestations and imaging characteristics of IOWFBs, misdiagnosis and missed diagnosis often occur during the initial visit. Delayed diagnosis may lead to a high risk of orbital infection caused by IOWFBs. Surgery is the treatment of choice for most patients;however, the missed diagnosis and residue of foreign bodies after previous surgery cannot be ignored. Therefore, an accurate diagnosis is governed by the detailed trauma history, careful ocular examination, close observation of clinical manifestations, correct imaging diagnosis [e.g., magnetic resonance imaging(MRI) or computerized tomography(CT)], and timely and completely elimination of IOWFBs.

Landscapes of Folk Beliefs Embodied by Wooden Arch Bridges in East Fujian,China

Cultures of folk beliefs in east Fujian Province were introduced and folk beliefs in the local area summarized as ancestor worship,female immortal worship,Taoist secular gods worship,nature worship and local sage worship.Distribution of wooden arch bridges in east Fujian was introduced,major functions of the wooden arch bridges summarized as traffic hub,repairing village environment,creating rural areas with a favorable Fengshui environment,and also god temple.Folk beliefs embodied in wooden arch bridges were classified and responsibilities of the gods and immortals introduced.It was summarized that wooden arch bridges in east Fujian were embodied with diversified folk beliefs,most of the gods and immortals were believed to be responsible for practical aspects of the locals' life,many gods and immortals were worshipped together,which shows the inclusiveness of folk beliefs in the local area,and contributes to main connotations and regional features of local folk belief cultures.

Wooden Wall Construction of Traditional Miao Stilted Buildings: A Case Study of Xijiang Miao Village in Guizhou

Wooden walls of traditional stilted buildings of Miao Nationality show outstanding regional characteristics. Taking the well-preserved traditional stilted buildings in Xijiang Miao Village for example, this paper focused on the construction units of the wooden wall, and the hierarchy of wooden wall construction, concluded spatial forms of the wooden wall, in order to explore the indigenous construction culture in the wooden wall system, protect and inherit the culture; seek for the ways of expressing national and regional characteristics in the modern architectural designs of relevant cultural regions.

Spatial Interpretation of the Wooden Arch Gallery Bridges in Northeast Fujian from the Perspective of Behavior Space

On the basis of investigating wooden arch gallery bridges in China,perspectives of researchers studying wooden arch gallery bridges in northeast Fujian Province were reviewed,and the innovativeness of researches from the perspectives of cultural geography and significance of wooden arch gallery bridge for the locals’behaviors was proposed.Youxi Village in Qinyang Town,Shouning County,known as the Village of Gallery Bridge,was taken for example to analyze physical,spiritual and social properties of its wooden arch gallery bridge spaces.Wooden arch gallery bridge is an effective boundary point of settlement space,constituting natural ecological spaces and man-made physical spaces of rural settlements.And as a type of building space,wooden arch gallery bridge expands settlement spaces,contributes to settlement cultures,and enriches spiritual connotations of regional cultures.In northeast Fujian,wooden arch gallery bridge is an important social space for the locals’daily life by carrying on living,production and spiritual functions.

Heritage and Development of Ningbo Bone-Inlaid Wooden Works

Bone-inlaid wooden art is a very old artistic form that has a strong artistic appeal and local flavor. It can be traced back to the Longshan Culture in the late Neolithic Age, and becomes mature in the late Qing Dynasty. As time lapses, however, bone-inlaid wooden art is gradually out of sight. It was not until June 2008 when Ningbo bone-inlaid wooden art was included in the "second batch of national intangible cultural heritage protection list" that it once again caused social concern. Ningbo bone-inlaid wooden art has a long history, and its craftsmanship is complex. Many bone-inlaid wooden works in Ningbo have been damaged more or less. Therefore, workers in the field of heritage protection should undertake a more systematic protection and repair work based on scientific research, making contributions to the heritage and development of intangible cultural heritage.

Wooden foreign body impalement through the right shoulder region–an unusual penetrating injury:A case report

BACKGROUND Impalement of the body is a rare injury and comes with varied presentation.There is no set classification or defined protocols for managing this injury.This case report aims to create awareness among trauma surgeons about unusual presentation and management of such case.CASE SUMMARY A 45-year-old man presented to the emergency department with a sharp penetrating wooden plank at right clavicular region between the neck and shoulder following a road traffic accident.The vehicle had crashed into a roadside wooden hut,thus causing an impalement injury.He was meticulously worked up and taken to emergency theatre.The wooden plank was removed and the wound healed uneventfully.Postoperatively,he had fairly good shoulder function and was able to return back to work successfully.CONCLUSION Each impalement injury brings in challenges in management as no two cases are the same.The varied presentation and risks involved should be known to medical professionals handling the emergency.Coordinated multidisciplinary team approach is needed for successful outcome.

Construction Techniques of Wooden Dwelling Column Frame in Tibetan Regions of Yunnan Province, China

Column frame of folk dwellings with slope roof in Tibetan regions of Yunnan Province was studied. Through field investigation and surveying, column frame timber, main components and construction techniques were introduced. The research showed that column frame of slope-roof dwelling in Tibetan regions of Yunnan is a regional construction technique using timber as the major materials, tenon-and-mortise work as the major connection, characterized by excellent structural expandability, and shock resistance, but it has no quantitative technical support. Scientific and systemic study is needed to improve safeness and livability of local dwellings.

Residential Wooden Houses Built in Small Towns of Lublin Region in 1918-1939

The housing situation just after the First World War was very bad.This was due,among other things,to the huge war damage of buildings as well as years of neglecting the construction movement during 123 years of partitioning.Not only public buildings were destroyed,but most of all housing development,which at that time in the Lublin region mostly had a wooden structure.The article presents a part of a comprehensive study of the wooden architecture of the interwar period of small towns in the Lublin region.Representative examples from each type of object were discussed.

Application of Management Information System Planning in Concurrent Engineering for Wooden Furniture Industry

This research is a development in management information system （MIS） planning based on operation analysis and development according to concurrent engineering approach and reestablishment of database management. According to our case study industry, such industry currently used traditional network systems such as LAN, and ＂Bus Network＂ Network Topology. Client/Server distributed computing has a problem with database management in data redundancy, data inconsistency, and data independency. For Network Topology, Bus Network has problem with multitasking since the network are able to handle only a set of data at a time so the traffic problem will occur when multiple users request for the service. Thus, such condition is inconsistent with concurrent engineering which must be able to access the data simultaneously. As a consequence, this study develops a network system, network system of working system, using LAN and ＂Star Network＂ network topology. The file server processing distributed is an application while database is stored in host computer or file server but the data will be processed in users＇ computer. When the user needs to access the data, file server will send it to the user and the user can further analysis or manage such data in the user computer, so called ＂Hierarchical Database Model＂. Hierarchical database structure is easily developed like general organization command structure with different level of responsibility. In details, the data level in the database is divided into three levels including DBI, DB2, and DB3, so development of simultaneously systemic flow and access of various critical data is performed in parallel. Furthermore, this is consistent with access of all three data levels including： Level 1 is overall dataflow of both inside and outside the organization; Level 2 is dataflow of each division in the organization; and Level 3 is dataflow of subunit in each division in the organization. After systemize flow and access of data with concurrent engineering as mentioned, it provided optimal efficiency in the whole production system management reducing loss in the whole system of the organization

Development of a Hybrid Pin Joint with a Compressed Wooden Dowel and Metal Pipe

A newly developed hybrid pin (HP), composed of a compressed wooden dowel inserted into a stainless steel pipe is suggested in this research. This configuration is expected to grant high stiffness by bending performance of the metal pipe and rich ductility through shear deformation of compressed wooden dowel without brittle split of the joint member. Experimental tests were performed in order to verify your assumptions and pursue an optimum design. Double shear test perpendicular to the grain of HP was conducted with parameter of thickness and loading direction for base member for pin’s diameter. Rotational test for mortise and tenon joint inserted with HP was performed in order to evaluate the moment resisting performance. Consequently, the hybrid pin showed satisfactory performance as shear type fastener by virtues of not only relatively high stiffness but also rich ductility originated from the properties of each component, stain less steel pipe and compressed wood.