Purpose:To reveal the research hotpots and relationship among three research hot topics in b iomedicine,namely CRISPR,iPS(induced Pluripotent Stem)cell and Synthetic biology.Design/methodology/approach:We set up their...Purpose:To reveal the research hotpots and relationship among three research hot topics in b iomedicine,namely CRISPR,iPS(induced Pluripotent Stem)cell and Synthetic biology.Design/methodology/approach:We set up their keyword co-occurrence networks with using three indicators and information visualization for metric analysis.Findings:The results reveal the main research hotspots in the three topics are different,but the overlapping keywords in the three topics indicate that they are mutually integrated and interacted each other.Research limitations:All analyses use keywords,without any other forms.Practical implications:We try to find the information distribution and structure of these three hot topics for revealing their research status and interactions,and for promoting biomedical developments.Originality/value:We chose the core keywords in three research hot topics in biomedicine by using h-index.展开更多
The aim of the paper is to identify and explore leading thematic areas within the research field related to innovation ecosystem.Based on data from the Web of Science database,the keywords frequency and its co-occurre...The aim of the paper is to identify and explore leading thematic areas within the research field related to innovation ecosystem.Based on data from the Web of Science database,the keywords frequency and its co-occurrence frequency pair were analyzed,and the theory of mapping knowledge domains was used to visualize the keywords co-occurrence network in innovation ecosystem to make further research of the heated issues.The findings indicate that the research scope involved in innovation ecosystem research is broad,and research content focus on micro and macro levels.According to the results of keywords co-occurrence analysis of different stages,innovation,network,knowledge,strategy,open innovation,value creation are the most important issues to innovation ecosystem research,given their position and role in the research network.The paper generates the added value mainly from the point of view of theory development.展开更多
Inversion tillage with straw amendment is widely applied in northeastern China, and it can substantially increase the storage of carbon and improve multiple subsoil functions. Soil microorganisms are believed to be th...Inversion tillage with straw amendment is widely applied in northeastern China, and it can substantially increase the storage of carbon and improve multiple subsoil functions. Soil microorganisms are believed to be the key to this process,but research into their role in subsoil amelioration is limited. Therefore, a field experiment was conducted in 2018 in a region in northeastern China with Hapli-Udic Cambisol using four treatments: conventional tillage(CT, tillage to a depth of 15 cm with no straw incorporation), straw incorporation with conventional tillage(SCT, tillage to a depth of 15 cm),inversion tillage(IT, tillage to a depth of 35 cm) and straw incorporation with inversion tillage(SIT, tillage to a depth of 35 cm). The soils were managed by inversion to a depth of 15 or 35 cm every year after harvest. The results indicated that SIT improved soil multi-nutrient cycling variables and increased the availability of key nutrients such as soil organic carbon, total nitrogen, available nitrogen, available phosphorus and available potassium in both the topsoil and subsoil.In contrast to CT and SCT, SIT created a looser microbial network structure but with highly centralized clusters by reducing the topological properties of average connectivity and node number, and by increasing the average path length and the modularity. A Random Forest analysis found that the average path length and the clustering coefficient were the main determinants of soil multi-nutrient cycling. These findings suggested that SIT can be an effective option for improving soil multi-nutrient cycling and the structure of microbial networks, and they provide crucial information about the microbial strategies that drive the decomposition of straw in Hapli-Udic Cambisol.展开更多
Denitrifying bacteria in epiphytic biofilms play a crucial role in nitrogen cycle in aquatic habitats.However,little is known about the connection between algae and denitrifying bacteria and their assembly processes i...Denitrifying bacteria in epiphytic biofilms play a crucial role in nitrogen cycle in aquatic habitats.However,little is known about the connection between algae and denitrifying bacteria and their assembly processes in epiphytic biofilms.Epiphytic biofilms were collected from submerged macrophytes(Patamogeton lucens and Najas marina L.)in the Caohai Lake,Guizhou,SW China,from July to November 2020 to:(1)investigate the impact of abiotic and biotic variables on denitrifying bacterial communities;(2)investigate the temporal variation of the algae-denitrifying bacteria co-occurrence networks;and(3)determine the contribution of deterministic and stochastic processes to the formation of denitrifying bacterial communities.Abiotic and biotic factors influenced the variation in the denitrifying bacterial community,as shown in the Mantel test.The co-occurrence network analysis unveiled intricate interactions among algae to denitrifying bacteria.Denitrifying bacterial community co-occurrence network complexity(larger average degrees representing stronger network complexity)increased continuously from July to September and decreased in October before increasing in November.The co-occurrence network complexity of the algae and nirS-encoding denitrifying bacteria tended to increase from July to November.The co-occurrence network complexity of the algal and denitrifying bacterial communities was modified by ammonia nitrogen(NH_(4)^(+)-N)and total phosphorus(TP),pH,and water temperature(WT),according to the ordinary least-squares(OLS)model.The modified stochasticity ratio(MST)results reveal that deterministic selection dominated the assembly of denitrifying bacterial communities.The influence of environmental variables to denitrifying bacterial communities,as well as characteristics of algal-bacterial co-occurrence networks and the assembly process of denitrifying bacterial communities,were discovered in epiphytic biofilms in this study.The findings could aid in the appropriate understanding and use of epiphytic biofilms denitrification function,as well as the enhancement of water quality.展开更多
The co-occurrence of bacteria and microeukaryote species is a ubiquitous ecological phenomenon,but there is limited cross-domain research in aquatic environments.We conducted a network statistical analysis and visuali...The co-occurrence of bacteria and microeukaryote species is a ubiquitous ecological phenomenon,but there is limited cross-domain research in aquatic environments.We conducted a network statistical analysis and visualization of microbial cross-domain co-occurrence patterns based on DNA sampling of a typical subtropical bay during four seasons,using high-throughput sequencing of both 18S rRNA and 16S rRNA genes.First,we found obvious relationships between network stability and network complexity indices.For example,increased cooperation and modularity were found to weaken the stability of cross-domain networks.Secondly,we found that bacterial operational taxonomic units(OTUs)were the most important contributors to network complexity and stability as they occupied more nodes,constituted more keystone OTUs,built more connections,more importantly,ignoring bacteria led to greater variation in network robustness.Gammaproteobacteria,Alphaproteobacteria,Bacteroidetes,and Actinobacteria were the most ecologically important groups.Finally,we found that the environmental drivers most associated with cross-domain networks varied across seasons(in detail,the network in January was primarily constrained by temperature and salinity,the network in April was primarily constrained by depth and temperature,the network in July was mainly affected by depth,temperature,and salinity,depth was the most important factor affecting the network in October)and that environmental influence was stronger on bacteria than on microeukaryotes.展开更多
The Western Subarctic Gyre(WSG)is one of the two gyre-systems in the subarctic North Pacific known for high nutrient and low-chlorophyll waters.However,the bacterioplankton in marine water of this area,either in terms...The Western Subarctic Gyre(WSG)is one of the two gyre-systems in the subarctic North Pacific known for high nutrient and low-chlorophyll waters.However,the bacterioplankton in marine water of this area,either in terms of the taxonomic composition or functional structure,remains relatively unexplored.A total of 22 sampling sites from two water layers(surface water,SW and 50-m layer water,FW)were collected in this area.The physiochemical parameters of waters,Synechococcus,and bacterial density,as well as the bacterioplankton community composition and distribution pattern,were analyzed.The nutrient concentrations of DIN,DIP,and DSi,Chl-a concentration,and the average abundance of heterobacteria in FW were higher than those in SW.However,temperature and the average abundance of Synechococcus and pico-eukaryotes were higher in SW.A total of 3269 OTUs were assigned,and 2123OTUs were commonly shared;moreover,similar alpha diversity patterns were observed in both SW and FW.The bacterioplankton community showed significantly obvious correlation with salinity,DIP,DIN,and Chl a in both SW and FW.Proteobacteria,Cyanobacteria,Bacteroidota,Actinobacteriota,and Firmicutes were the main phyla while Synechococcus_CC9902,Psychrobacter,and Sulfitobacter were the dominant genera in each sampling site.Most correlations that happened between the OTUs in the cooccurrence network were positive and inter-module.Higher edges and graph density were found in SW,indicating that more correlations occurred,and the community was more complex in SW.This study provided novel knowledge on the bacterioplankton community structure and the correlation characteristics in WSG.展开更多
[目的/意义]在人工智能技术及应用快速发展与深刻变革背景下,机器学习领域不断出现新的研究主题和方法,深度学习和强化学习技术持续发展。因此,有必要探索不同领域机器学习研究主题演化过程,并识别出热点与新兴主题。[方法/过程]本文以...[目的/意义]在人工智能技术及应用快速发展与深刻变革背景下,机器学习领域不断出现新的研究主题和方法,深度学习和强化学习技术持续发展。因此,有必要探索不同领域机器学习研究主题演化过程,并识别出热点与新兴主题。[方法/过程]本文以图书情报领域中2011—2022年Web of Science数据库中的机器学习研究论文为例,融合LDA和Word2vec方法进行主题建模和主题演化分析,引入主题强度、主题影响力、主题关注度与主题新颖性指标识别热点主题与新兴热点主题。[结果/结论]研究结果表明,(1)Word2vec语义处理能力与LDA主题演化能力的结合能够更加准确地识别研究主题,直观展示研究主题的分阶段演化规律;(2)图书情报领域的机器学习研究主题主要分为自然语言处理与文本分析、数据挖掘与分析、信息与知识服务三大类范畴。各类主题之间的关联性较强,且具有主题关联演化特征;(3)设计的主题强度、主题影响力和主题关注度指标及综合指标能够较好地识别出2011—2014年、2015—2018年和2019—2022年3个不同周期阶段的热点主题。展开更多
This study investigates the feasibility of applying complex networks to fine-grained language classification and of employing word co-occurrence networks based on parallel texts as a substitute for syntactic dependenc...This study investigates the feasibility of applying complex networks to fine-grained language classification and of employing word co-occurrence networks based on parallel texts as a substitute for syntactic dependency networks in complex-network-based language classification.14 word co-occurrence networks were constructed based on parallel texts of 12 Slavic languages and 2 non-Slavic languages,respectively.With appropriate combinations of major parameters of these networks,cluster analysis was able to distinguish the Slavic languages from the non-Slavic and correctly group the Slavic languages into their respective sub-branches.Moreover,the clustering could also capture the genetic relationships of some of these Slavic languages within their sub-branches.The results have shown that word co-occurrence networks based on parallel texts are applicable to fine-grained language classification and they constitute a more convenient substitute for syntactic dependency networks in complex-network-based language classification.展开更多
This study focuses on the analysis of the Chinese composition writing performance of fourth,fifth,and sixth grade students in 16 selected schools in Longhua District,Shenzhen during the spring semester of 2023.Using L...This study focuses on the analysis of the Chinese composition writing performance of fourth,fifth,and sixth grade students in 16 selected schools in Longhua District,Shenzhen during the spring semester of 2023.Using LIWC(Linguistic Inquiry and Word Count)as a text analysis tool,the study explores the impact of LIWC categories on writing performance which is scaled by score.The results show that the simple LIWC word categories have a significant positive influence on the composition scores of lower-grade students;while complex LIWC word categories have a significant negative influence on the composition scores of lower-grade students but a significant positive influence on the composition scores of higher-grade students.Process word categories have a positive influence on the composition scores of all three grades,but the impact of complex process word categories increases as the grade level rises.展开更多
基金the National Natural Science Foundation of China Grant 71673131 for financial support
文摘Purpose:To reveal the research hotpots and relationship among three research hot topics in b iomedicine,namely CRISPR,iPS(induced Pluripotent Stem)cell and Synthetic biology.Design/methodology/approach:We set up their keyword co-occurrence networks with using three indicators and information visualization for metric analysis.Findings:The results reveal the main research hotspots in the three topics are different,but the overlapping keywords in the three topics indicate that they are mutually integrated and interacted each other.Research limitations:All analyses use keywords,without any other forms.Practical implications:We try to find the information distribution and structure of these three hot topics for revealing their research status and interactions,and for promoting biomedical developments.Originality/value:We chose the core keywords in three research hot topics in biomedicine by using h-index.
文摘The aim of the paper is to identify and explore leading thematic areas within the research field related to innovation ecosystem.Based on data from the Web of Science database,the keywords frequency and its co-occurrence frequency pair were analyzed,and the theory of mapping knowledge domains was used to visualize the keywords co-occurrence network in innovation ecosystem to make further research of the heated issues.The findings indicate that the research scope involved in innovation ecosystem research is broad,and research content focus on micro and macro levels.According to the results of keywords co-occurrence analysis of different stages,innovation,network,knowledge,strategy,open innovation,value creation are the most important issues to innovation ecosystem research,given their position and role in the research network.The paper generates the added value mainly from the point of view of theory development.
基金funded by the National Key Research and Development Program of China (2022YFD1500100)the Strategic Priority Research Program of the Chinese Academy of Sciences (XDA28070100)+1 种基金the National Natural Science Foundation of China (41807085)the earmarked fund for China Agriculture Research System (CARS04)。
文摘Inversion tillage with straw amendment is widely applied in northeastern China, and it can substantially increase the storage of carbon and improve multiple subsoil functions. Soil microorganisms are believed to be the key to this process,but research into their role in subsoil amelioration is limited. Therefore, a field experiment was conducted in 2018 in a region in northeastern China with Hapli-Udic Cambisol using four treatments: conventional tillage(CT, tillage to a depth of 15 cm with no straw incorporation), straw incorporation with conventional tillage(SCT, tillage to a depth of 15 cm),inversion tillage(IT, tillage to a depth of 35 cm) and straw incorporation with inversion tillage(SIT, tillage to a depth of 35 cm). The soils were managed by inversion to a depth of 15 or 35 cm every year after harvest. The results indicated that SIT improved soil multi-nutrient cycling variables and increased the availability of key nutrients such as soil organic carbon, total nitrogen, available nitrogen, available phosphorus and available potassium in both the topsoil and subsoil.In contrast to CT and SCT, SIT created a looser microbial network structure but with highly centralized clusters by reducing the topological properties of average connectivity and node number, and by increasing the average path length and the modularity. A Random Forest analysis found that the average path length and the clustering coefficient were the main determinants of soil multi-nutrient cycling. These findings suggested that SIT can be an effective option for improving soil multi-nutrient cycling and the structure of microbial networks, and they provide crucial information about the microbial strategies that drive the decomposition of straw in Hapli-Udic Cambisol.
基金Supported by the National Natural Science Foundation of China(No.41867056)the Guizhou Provincial Key Technology R&D Program(Nos.2021470,2023216)。
文摘Denitrifying bacteria in epiphytic biofilms play a crucial role in nitrogen cycle in aquatic habitats.However,little is known about the connection between algae and denitrifying bacteria and their assembly processes in epiphytic biofilms.Epiphytic biofilms were collected from submerged macrophytes(Patamogeton lucens and Najas marina L.)in the Caohai Lake,Guizhou,SW China,from July to November 2020 to:(1)investigate the impact of abiotic and biotic variables on denitrifying bacterial communities;(2)investigate the temporal variation of the algae-denitrifying bacteria co-occurrence networks;and(3)determine the contribution of deterministic and stochastic processes to the formation of denitrifying bacterial communities.Abiotic and biotic factors influenced the variation in the denitrifying bacterial community,as shown in the Mantel test.The co-occurrence network analysis unveiled intricate interactions among algae to denitrifying bacteria.Denitrifying bacterial community co-occurrence network complexity(larger average degrees representing stronger network complexity)increased continuously from July to September and decreased in October before increasing in November.The co-occurrence network complexity of the algae and nirS-encoding denitrifying bacteria tended to increase from July to November.The co-occurrence network complexity of the algal and denitrifying bacterial communities was modified by ammonia nitrogen(NH_(4)^(+)-N)and total phosphorus(TP),pH,and water temperature(WT),according to the ordinary least-squares(OLS)model.The modified stochasticity ratio(MST)results reveal that deterministic selection dominated the assembly of denitrifying bacterial communities.The influence of environmental variables to denitrifying bacterial communities,as well as characteristics of algal-bacterial co-occurrence networks and the assembly process of denitrifying bacterial communities,were discovered in epiphytic biofilms in this study.The findings could aid in the appropriate understanding and use of epiphytic biofilms denitrification function,as well as the enhancement of water quality.
基金Supported by the National Natural Science Foundation of China(Nos.42141003,42176147)the National Key Research and Development Program of China(No.2022YFF0802204)the Natural Science Foundation of Fujian Province of China(No.2021J01025)。
文摘The co-occurrence of bacteria and microeukaryote species is a ubiquitous ecological phenomenon,but there is limited cross-domain research in aquatic environments.We conducted a network statistical analysis and visualization of microbial cross-domain co-occurrence patterns based on DNA sampling of a typical subtropical bay during four seasons,using high-throughput sequencing of both 18S rRNA and 16S rRNA genes.First,we found obvious relationships between network stability and network complexity indices.For example,increased cooperation and modularity were found to weaken the stability of cross-domain networks.Secondly,we found that bacterial operational taxonomic units(OTUs)were the most important contributors to network complexity and stability as they occupied more nodes,constituted more keystone OTUs,built more connections,more importantly,ignoring bacteria led to greater variation in network robustness.Gammaproteobacteria,Alphaproteobacteria,Bacteroidetes,and Actinobacteria were the most ecologically important groups.Finally,we found that the environmental drivers most associated with cross-domain networks varied across seasons(in detail,the network in January was primarily constrained by temperature and salinity,the network in April was primarily constrained by depth and temperature,the network in July was mainly affected by depth,temperature,and salinity,depth was the most important factor affecting the network in October)and that environmental influence was stronger on bacteria than on microeukaryotes.
基金Supported by the National Key Research and Development Program of China(No.2019YFD0901401)the Natural Science Foundation of Shandong Province(No.ZR202102280248)+1 种基金the National Natural Science Foundation of China(No.81900630)the Outstanding Youth Project of Yunnan Provincial Department of Science and Technology(No.2019F1019)。
文摘The Western Subarctic Gyre(WSG)is one of the two gyre-systems in the subarctic North Pacific known for high nutrient and low-chlorophyll waters.However,the bacterioplankton in marine water of this area,either in terms of the taxonomic composition or functional structure,remains relatively unexplored.A total of 22 sampling sites from two water layers(surface water,SW and 50-m layer water,FW)were collected in this area.The physiochemical parameters of waters,Synechococcus,and bacterial density,as well as the bacterioplankton community composition and distribution pattern,were analyzed.The nutrient concentrations of DIN,DIP,and DSi,Chl-a concentration,and the average abundance of heterobacteria in FW were higher than those in SW.However,temperature and the average abundance of Synechococcus and pico-eukaryotes were higher in SW.A total of 3269 OTUs were assigned,and 2123OTUs were commonly shared;moreover,similar alpha diversity patterns were observed in both SW and FW.The bacterioplankton community showed significantly obvious correlation with salinity,DIP,DIN,and Chl a in both SW and FW.Proteobacteria,Cyanobacteria,Bacteroidota,Actinobacteriota,and Firmicutes were the main phyla while Synechococcus_CC9902,Psychrobacter,and Sulfitobacter were the dominant genera in each sampling site.Most correlations that happened between the OTUs in the cooccurrence network were positive and inter-module.Higher edges and graph density were found in SW,indicating that more correlations occurred,and the community was more complex in SW.This study provided novel knowledge on the bacterioplankton community structure and the correlation characteristics in WSG.
文摘[目的/意义]在人工智能技术及应用快速发展与深刻变革背景下,机器学习领域不断出现新的研究主题和方法,深度学习和强化学习技术持续发展。因此,有必要探索不同领域机器学习研究主题演化过程,并识别出热点与新兴主题。[方法/过程]本文以图书情报领域中2011—2022年Web of Science数据库中的机器学习研究论文为例,融合LDA和Word2vec方法进行主题建模和主题演化分析,引入主题强度、主题影响力、主题关注度与主题新颖性指标识别热点主题与新兴热点主题。[结果/结论]研究结果表明,(1)Word2vec语义处理能力与LDA主题演化能力的结合能够更加准确地识别研究主题,直观展示研究主题的分阶段演化规律;(2)图书情报领域的机器学习研究主题主要分为自然语言处理与文本分析、数据挖掘与分析、信息与知识服务三大类范畴。各类主题之间的关联性较强,且具有主题关联演化特征;(3)设计的主题强度、主题影响力和主题关注度指标及综合指标能够较好地识别出2011—2014年、2015—2018年和2019—2022年3个不同周期阶段的热点主题。
基金supported by the National Social Science Foundation of China (09BYY024 and 11&ZD188)
文摘This study investigates the feasibility of applying complex networks to fine-grained language classification and of employing word co-occurrence networks based on parallel texts as a substitute for syntactic dependency networks in complex-network-based language classification.14 word co-occurrence networks were constructed based on parallel texts of 12 Slavic languages and 2 non-Slavic languages,respectively.With appropriate combinations of major parameters of these networks,cluster analysis was able to distinguish the Slavic languages from the non-Slavic and correctly group the Slavic languages into their respective sub-branches.Moreover,the clustering could also capture the genetic relationships of some of these Slavic languages within their sub-branches.The results have shown that word co-occurrence networks based on parallel texts are applicable to fine-grained language classification and they constitute a more convenient substitute for syntactic dependency networks in complex-network-based language classification.
文摘This study focuses on the analysis of the Chinese composition writing performance of fourth,fifth,and sixth grade students in 16 selected schools in Longhua District,Shenzhen during the spring semester of 2023.Using LIWC(Linguistic Inquiry and Word Count)as a text analysis tool,the study explores the impact of LIWC categories on writing performance which is scaled by score.The results show that the simple LIWC word categories have a significant positive influence on the composition scores of lower-grade students;while complex LIWC word categories have a significant negative influence on the composition scores of lower-grade students but a significant positive influence on the composition scores of higher-grade students.Process word categories have a positive influence on the composition scores of all three grades,but the impact of complex process word categories increases as the grade level rises.