Evaluation of wound healing and antimicrobial potentials of Ixora coccinea root extract

Objective:To evaluate the wound healing and antimicrobial activity of root extracts of Ixora coccinea(I.coccinea).Methods:To investigate the wound healing efficacy of root extract of I.coccinea Linn,five groups of animals were divided each containing six animals.Two wound models including incision and excision wound models were used in this study.The parameters studied were tensile strength on incision wound model and in terms of wound contraction for excision wound model were compared with standard Nitrofurazone(NFZ) ointment(0.2%w/w). Six extracts(ethanol.aqueous,petroleum ether,benzene,chloroform and ethyl acetate ) of I. coccinea were screened for in vitro growth inhibiting activity against different bacterial strains riz. Staphylococcia aureus.Bacillus pumilius,Enterococcus facialis.Escherichia coli,Salmonella typhi and Pseudomonas aeruginosa and fungi Candida albicans and Aspergillus niger were compared with the standard drugs ciprofloxacin and chloramphenicol for antibacterial and griscofulvin for antifungal screening.The serial dilution and cup(or) well plate methods were used for the antimicrobial study and MIC was determined.Results:The ethanolic extract showed significant (P【0.001) wound healing activity when compared to standard drug NFZ with respect to normal control group.Amongst all.ethanolic extract showed highly significant antibacterial activity against all bacterial strains used in this study when compared to standard.The aqueous extract showed moderate significant inhibition against all bacterial strains when compared to standard. All the extracts were shown negligible activity against the fungal strains used in this study. Conclusions:The ethanolic root extract of I.coccinea showed pronounced wound healing and antibacterial activity.The probable reason to heal the wound was that the external application of the extract prevented the microbes to invade through the wound thus the protection of wound occurs against the infection of the various organisms.

Keladi candik(Alocasia longiloba Miq.) petiole extracts promote wound healing in a full thickness excision wound model in rats

Objective: To investigate the wound-healing effect of Alocasia longiloba(A. longiloba) petiole extract on wounds in rats.Methods: Twenty-two male Sprague-dawley rats were randomly assigned to receive 10% solcoseryl gel, phosphate buffer saline, 50% ethanol, 95% ethanol and hexane extracts of A. longiloba at 1.5%, 3% and 6% doses, respectively. A full thicknesses wound(6 mm) was created on the dorsal of the rat; and all rats were applied with the extract solutions, 10% solcoseryl gel and phosphate buffer saline once a day topically until day 12. The wound was photographed on day 1, 6 and 12, and the percentage of wound contraction was calculated. On day 12, rats were sacrificed and histological examination of granulation tissue was carried out using haematoxylin & eosin and Masson's Trichrome stain to determine the wound healing effect.Results: In this study, 6% of 50% and 95% ethanol extracts of A. longiloba showed 82.50% and 82.32% wound contraction, respectively, and were comparable with 10% solcoseryl gel(82.30%). Meanwhile, phosphate buffer saline treated group showed the lowest wound contraction(69.86%). Histological assessment of wound treated with 6% of 95% ethanol extract of A. longiloba showed distinct epidermal and dermal layer, higher proliferation of fibroblast and more angiogenesis with collagen compared to other wound treated groups.Conclusions: A. longiloba petiole extracts have a wound healing potential and 6% of 95% ethanol extract of A. longiloba is more effective. Further studies are required to understand the wound healing mechanism of action of the extract.

富血小板纤维蛋白植入牙槽窝保存术对后牙拔除患者牙槽嵴吸收的影响

目的 探讨后牙拔除富血小板纤维蛋白(PRF)植入牙槽窝保存术对患者牙槽嵴吸收的影响。方法 回顾性选取2021年10月至2023年10月宣城市人民医院收治的后牙拔除患者66例作为研究对象,依据拔牙后牙槽窝保存术所用种植材料不同分为PRF植入牙槽窝保存术组(PRF植入组)、海奥胶原膜植入牙槽窝保存术组(海奥胶原膜植入组)两组,每组各33例。比较两组患者的拟可种种植体长度、直径分布情况、早期软组织愈合情况、骨密度、牙槽嵴宽度、牙槽嵴高度、牙槽嵴骨量、牙龈乳头高度、疼痛程度、矿化骨部分填充量和创面愈合时间。结果 PRF植入组患者的拟可种种植体长度8 mm比例为9.09%,低于海奥胶原膜植入组(45.45%),10、12 mm比例分别为63.64%、27.27%,均明显高于海奥胶原膜植入组(42.42%,12.12%),差异均有统计学意义(P<0.05)。PRF植入组患者拟可种种植体的直径4.1 mm比例为39.39%,明显低于海奥胶原膜植入组(66.67%),4.8 mm比例为60.61%,明显高于海奥胶原膜植入组(33.33%),差异均有统计学意义(P<0.05)。两组患者早期软组织愈合情况比较,差异无统计学意义(P>0.05)。PRF植入组患者修复后的骨密度、牙槽嵴宽度、牙槽嵴高度、临床附着水平(CAL)均高于海奥胶原膜植入组,牙龈退缩低于海奥胶原膜植入组,差异均有统计学意义(P<0.05)。PRF植入组患者的牙龈乳头高度0~Ⅰ级比例为60.61%,明显高于海奥胶原膜植入组(36.36%),Ⅱ级、Ⅲ级、Ⅳ级比例分别为33.33%,3.03%,3.03%,均明显低于海奥胶原膜植入组(45.45%,12.12%,6.06%),差异有统计学意义(P<0.05)。PRF植入组患者的视觉模拟评分法(visual analogue scale, VAS)评分明显低于海奥胶原膜植入组,矿化骨部分填充量明显多于海奥胶原膜植入组,创面愈合时间明显短于海奥胶原膜植入组,差异均有统计学意义(P<0.05)。结论 后牙拔除PRF植入牙槽窝保存术较海奥胶原膜植入牙槽窝保存术更能减少患者牙槽嵴的吸收。

艾叶/茶叶水提物促进皮肤感染模型大鼠创面愈合的协同作用及机制研究

目的探讨艾叶水提物和茶叶水提物对皮肤感染模型大鼠的协同治疗作用及其机制。方法实验分两个部分进行:第一部分将皮肤感染模型大鼠随机分为模型组、西药组、艾叶水提物组、茶叶水提物组、2∶1组(艾叶水提物与茶叶水提物质量比为2∶1)、1∶1组(艾叶水提物与茶叶水提物质量比为1∶1)和1∶2组(艾叶水提物与茶叶水提物质量比为1∶2),每组7只。比较治疗前后各组大鼠创面面积;ELISA法检测各组大鼠血清肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)、白细胞介素(interleukin,IL)-1β、IL-18水平。第二部分随机选取7只大鼠为正常组,将皮肤感染模型大鼠随机分为模型组、2∶1组(艾叶水提物与茶叶水提物质量比为2∶1),每组7只。Western blot法检测各组大鼠皮肤组织黑色素瘤缺乏因子2(absent in melanoma 2,AIM2)、含有CARD的凋亡相关斑点样蛋白(apoptosis-associated speck-like protein containing CARD,ASC)和Caspase-1蛋白表达水平;ELISA法检测各组大鼠血清AIM2、ASC、Caspase-1及IL-1β、IL-18水平。结果第一部分,与模型组比较,西药组、艾叶水提物组、茶叶水提物组和3个不同比例组感染创口收缩明显,创面面积明显减小,且促炎因子TNF-α、IL-1β、IL-18的表达水平均显著降低(P<0.05)。其中西药组、2∶1组效果更明显,两组促炎因子TNF-α、IL-1β水平比较,差异无统计学意义(P>0.05)。第二部分,与正常组比较,模型组感染创面组织中AIM2、ASC、Caspase-1蛋白表达水平和血清IL-1、IL-18水平均显著升高(P<0.05)。与模型组比较,2∶1组感染创面组织中AIM2、ASC、Caspase-1蛋白表达水平和血清IL-1、IL-18水平均显著降低(P<0.05)。结论艾叶水提物和茶叶水提物对治疗皮肤感染模型大鼠存在协同作用,艾叶水提物与茶叶水提物质量比为2∶1是最优比例。其协同机制可能与AIM2炎症小体活化和细胞焦亡通路有关。

基于Akt/mTOR自噬通路介导NLRP3炎症小体失活探讨冲和膏促进糖尿病大鼠溃疡创面愈合的作用机制

目的探讨冲和膏通过蛋白激酶B(Akt)/哺乳动物雷帕霉素靶蛋白(mTOR)自噬通路介导核苷酸结合寡聚酸结构域样受体蛋白3(NLRP3)炎症小体失活对糖尿病大鼠溃疡创面愈合的作用机制。方法采用高糖高脂饲料喂养、腹腔注射链脲佐菌素(Streptozocin,STZ)联合皮肤缺损方法构建糖尿病溃疡SD大鼠创面模型36只,将大鼠随机分为模型组、冲和膏组和生长因子组,每组12只。另取12只SD大鼠构建普通创面模型作为空白组。空白组和模型组不进行药物干预,冲和膏组和生长因子组分别外敷冲和膏、生长因子凝胶,观察记录各组大鼠创面愈合情况。给药第7天和第14天分别取材,HE染色观察创面组织病理学改变,并进行成纤维细胞计数;ELISA法检测血清中白细胞介素(IL)-1β、IL-18、肿瘤坏死因子(TNF)-α水平;免疫组化检测创面肉芽组织自噬相关蛋白Beclin-1、LC3-Ⅱ表达水平;Western Blot法检测创面肉芽组织炎症小体相关蛋白NLRP3、含Caspase募集结构域的凋亡相关斑点样蛋白(ASC)、Caspase-1、Pro-Caspase-1及Akt/mTOR自噬通路相关蛋白Akt、p-Akt、mTOR、p-mTOR的表达情况。结果与空白组比较,模型组在第7天和第14天创面病理损伤修复延缓,单位面积成纤维细胞数量较少(P<0.01),炎症因子IL-1β、IL-18、TNF-α水平升高(P<0.01),创面组织中ASC、Pro-Caspase-1、Caspase-1、NLRP3表达水平升高(P<0.01),Beclin-1、LC3-Ⅱ以及mTOR、p-mTOR、Akt、p-Akt表达水平降低(P<0.01);与模型组比,第7天和第14天冲和膏组和生长因子组病理损伤明显改善,单位面积成纤维细胞数量明显增加(P<0.01),炎症因子IL-1β、IL-18、TNF-α水平明显降低(P<0.01),创面组织中ASC、Pro-Caspase-1、Caspase-1、NLRP3表达水平降低(P<0.01),Beclin-1、LC3-Ⅱ以及mTOR、p-mTOR、Akt、p-Akt表达水平升高(P<0.01,P<0.05)。结论冲和膏可降低炎症反应,促进成纤维细胞生成,调控Akt/mTOR通路介导NLRP3炎症小体失活,改善创面自噬水平,从而促进糖尿病大鼠溃疡创面愈合。

心脏植入式电子装置感染的诊治进展

随着心血管疾病发生率逐渐升高,心脏植入式电子装置(cardiac implantable electronic device, CIED)在临床治疗中的应用越来越广泛,CIED感染的发病率也随之升高。目前国际各大指南及共识均推荐CIED感染发生后应尽早彻底移除整个装置系统(包括脉冲发生器和电极),然而拔除电极的技术难度及风险较大,部分患者因生理或心理因素无法完成相关手术治疗,因此正确认识感染的发生机制、危险因素、治疗和预防,对临床正确处理CIED感染有重要意义。本文就近年CIED感染诊治进展进行综述。

血人参治疗创伤性慢性创面最佳活性部位的筛选及其成分分析

目的:研究血人参不同极性部位促创伤性慢性创面愈合效果,筛选出最佳活性部位并对其进行成分研究。方法:建立创伤性慢性创面,给予不同极性部位的血人参提取物,通过创面愈合情况与程度筛选出促创愈合的最佳活性部位;通过LC-MS对最佳活性部位进行全谱分析。结果:血人参不同极性部位提取物对创伤性慢性创面均有不同程度的治疗效果,其中石油醚萃取部位的治疗效果最佳;LC-MS全谱分析结果显示,石油醚萃取物中主要含大量的脂肪酰类、部分黄酮和异黄酮、羧酸及其衍生物、香豆素及其衍生物等物质。结论:血人参石油醚萃取物对大鼠创伤性慢性创面的治疗效果最佳,可能是其中的脂肪酰、黄酮、香豆素以及羧酸类化合物发挥合疗效的结果。

分心木水提物可溶性微针的制备及其对小鼠皮肤溃疡的治疗效果研究

【目的】筛选分心木水提物微针的制备处方,同时考察其对小鼠皮肤溃疡模型的有效性。【方法】采用两步离心法制备分心木可溶性微针,对微针基质材料处方进行筛选,并测定不同浓度分心木水提物微针释药量,得到最佳基质处方,最终用于评价微针质量;采用化学灼烧法建立小鼠皮肤溃疡模型,模型小鼠随机分为生理盐水组、2%分心木水提物溶液涂布组、2%分心木水提物微针给药组和5%分心木水提物微针给药组,间隔3 d给药1次,期间对小鼠溃疡进行大体形态观察及面积测量,并对造模第14天时溃疡皮肤的羟脯氨酸含量进行检测,对溃疡皮肤组织块进行石蜡包埋切片,HE染色,光镜观察病理组织学变化。【结果】最终筛选得到5%透明质酸作为分心木水提物微针制备基质材料,且后续质量评价结果好;动物试验结果发现,与生理盐水组相比,给药组小鼠皮肤溃疡创面愈合率均显著提高,不同浓度微针组小鼠第14天时溃疡皮肤中羟脯氨酸含量显著增加,病理组织学检查结果表现出更好的愈合效果。【结论】试验制得的分心木水提物微针可有效促进小鼠皮肤溃疡创面愈合。

美洲大蠊鲜虫提取物对慢性难愈合创面组织中PI3K、Akt、mTOR、VEGF蛋白表达的影响

目的:探索美洲大蠊鲜虫提取物对慢性难愈合创面的疗效与机制。方法:用BALB-3T3细胞进行细胞增殖、划痕和transwell实验,免疫印迹法检测细胞上清液中PI3K、Akt、mTOR和VEGF蛋白表达水平;构建大鼠慢性难愈合创面模型,在造模第0、4、8、12、15 d拍照记录创面,计算创面愈合率;每组随机抽取3只取材,用免疫印迹法检测组织中PI3K、Akt、mTOR和VEGF蛋白表达水平。结果:美洲大蠊鲜虫提取物显著促进BALB-3T3细胞的增殖和迁移(P<0.01),且细胞上清液中VEGF蛋白表达量上升(P<0.05);与模型组比较,美洲大蠊鲜虫提取物高、中、低剂量组、康复新液组和急性创面组创面愈合率增加(P<0.05或P<0.01);蛋白免疫印迹结果显示,与慢性模型组比较,美洲大蠊鲜虫提取物胶浆剂高、中、低剂量组、康复新液组和急性创面组PI3K、Akt、mTOR和VEGF蛋白表达量上升(P<0.05或P<0.01)。结论:美洲大蠊鲜虫提取物可促进BALB-3T3细胞的增殖和迁移,并上调PI3K、Akt、mTOR和VEGF蛋白表达来促进慢性难愈合创面的愈合。

Pharmacological studies on Buchanania lanzan Spreng.-A focus on wound healing with particular reference to anti-biofilm properties

Objective:To evaluate the wound healing activity of the methanolic root extract of Buchanania lanzan Spreng.(B.lanzan),with a focus on antimicrobial and anti-biofilm properties.Methods:The extract was evaluated for its wound healing properties(excision and incision models)as evident from the analysis of tensile strength and wound contraction.The extract was also screened for antibacterial properties against different Cram positive and Gram negative bacterial strains.B.lanzan was also studied for its effect on biofilm formation and disruption of preformed biofilms.The synergistic effect of B.lanzan was determined in combination with gentamicin.Results:Topical application of B.lanzan(10%w/w ointment)significantly increased(40.84%)the tensile strength in the incision wound model.B.lanzan also showed significant wound healing activity in excision model and such significant activity was observed from the 9th day.Whereas Soframycin displayed significant wound healing activity from the 6th day.It was found that root extracts of B.lanzan revealed significant inhibition against all tested pathogens.B.lanzan displayed antimicrobial activity against Cram positive(MIC 0.625 mg/mL)and Cram negative(MIC0.625-1.25 mg/mL).B.lanzan was able to reduce biofilm formation and also caused disruption of preformed biofilms in a manner similar to ciprofloxacin.However,gentamicin was found to be ineffective against biofilms formed by Gram negative organism.According to the fractional inhibitory concentration index,B.lanzan displayed synergistic activity when it was combined with gentamicin.Conclusions:From this study it may be concluded that the root extract of B.lanzan revealed significant wound healing potential,which was supported and well correlated with pronounced antibacterial activity of the tested plant parts.

高速涡轮机联合微创拔牙刀治疗阻生智齿患者的效果

目的:观察高速涡轮机联合微创拔牙刀治疗阻生智齿患者的效果。方法:回顾性分析2020年5月至2023年2月该院收治的126例阻生智齿患者的临床资料,按照治疗方法不同将其分为对照组和观察组各63例。对照组采用凿骨劈冠法治疗,观察组采用高速涡轮机联合微创拔牙刀治疗。比较两组拔牙相关指标(拔牙时间、牙窝损伤评分、张口受限度、面颊肿胀度)水平、拔牙后不同时间疼痛[视觉模拟评分法(VAS)]评分、拔牙前后龈沟液指标[前列腺素E_(2)(PGE_(2))、5-羟色胺(5-HT)、白细胞介素-6(IL-6)]水平,并发症发生率和伤口愈合优良率。结果:观察组拔牙时间短于对照组,牙窝损伤评分、张口受限度、面颊肿胀度均低于对照组,差异有统计学意义(P<0.05);拔牙后24、48、72 h,观察组VAS评分均低于对照组,差异有统计学意义(P<0.05);拔牙后,两组龈沟液PGE_(2)、5-HT、IL-6水平均高于拔牙前,但观察组低于对照组,差异有统计学意义(P<0.05);观察组并发症发生率为7.94%(5/63),低于对照组的25.40%(16/63),差异有统计学意义(P<0.05);观察组伤口愈合优良率为93.65%(59/63),高于对照组的80.95%(51/63),差异有统计学意义(P<0.05)。结论:高速涡轮机联合微创拔牙刀治疗阻生智齿患者可改善拔牙相关指标水平,降低疼痛评分、龈沟液指标水平和并发症发生率,提高伤口愈合优良率,效果优于凿骨劈冠法治疗。

苯扎氯铵溶液预防拔牙术后创口感染的临床评价

目的:探讨苯扎氯铵溶液对预防拔牙术后创口感染的临床效果。方法:选取2020年6月-2021年6月在我院行下颌阻生齿拔除术的180例患者,依照患者就诊顺序及个人意愿分为苯扎氯铵溶液组(n=60,术后含漱苯扎氯铵溶液)、生理盐水组(n=60,术后含漱生理盐水)和空白对照组(n=60,不用药物含漱),对比分析3组患者术后创口情况。结果:苯扎氯铵溶液组、生理盐水组和空白对照组发生创口感染者分别为0、5、12例,苯扎氯铵溶液组术后创口感染率明显低于其他2组(P<0.05)。苯扎氯铵溶液组术后7 d Negm分级2~4级的患者占比3.33%,生理盐水组占比26.67%,空白对照组占比35.00%,苯扎氯铵溶液组的Negm分级明显低于其他2组(P<0.05)。结论:使用苯扎氯铵溶液含漱可有效预防拔牙术后创口感染。

Quantitation of Polydeoxyribonucletides (PDRNs) in Human Placental Extract by Fluorescence Spectroscopy Using Ethidium Bromide

Characterization of an aqueous extract of human placenta, used as a licensed drug for wound healing, leads to the identification of several bioactive components including polydeoxyribonu-cleotides (PDRNs). PDRNs are mixture of DNA fragments of different molecular weight. A spectro-fluorimetric method of quantitation of PDRNs in the aqueous extract of human placenta by using ethidium bromide (EtBr) has been described here. It has been demonstrated by thin layer chromatography (TLC) followed by reversed phase HPLC that EtBr binds specifically with the PDRN fraction of the multi-component extract. The binding specificity of EtBr has been verified by the analysis of emission spectra of the extract. A concentration of 0.29 μg/ml EtBr exhibits a linear range of standard CT-DNA from 0.5 - 5 μg/ml of buffer (R2 = 0.992). The same concentration of EtBr shows a linear range of measurements of placenta extract from 5 - 35 μl/ml of buffer (R2 = 0.976). The points of the curve were the average of three sets where maximum variation observed was ±3%. PDRN content of the extract has been estimated based on the resultant fluorescence emission (after background correction) with respect to the standard calibration curve of calf thymus DNA (CT-DNA). Estimation of PDRN in a large number of batches of placenta extract (n = 100) has been done. The statistical analysis of the estimation was found to be significant and the lower and upper levels of PDRN were 158.30 and 239.03 μg/ml of the extract respectively. This easy-to-use method of estimation of PDRN in multi-component biological extract is reported for the first time. This will help in quantitation of PDRNs for other biological extracts.

Anti-Acinetobacter baumannii activity of Rumex crispus L. and Rumex sanguineus L. extracts

Objective:To examine the effect of Rumex crispus(R.crispus)and Rumex sanguineus(R.sanguineus)plant extracts against isolates of Acinetobacter baumannii(A.baumannii)from wounds,including multidrug-resistant strains.Methods:Six prepared Rumex extracts were subjected to liquid chromatography-tandem mass spectrometry.Antimicrobial activity of extracts and pure compounds(catechin,quercetin,isoquercitrin,emodin,and gallic acid)was examined by a microtiter plate method,while for determination of compound binary combinations activity a checkerboard method was applied.Active fractions of extracts were detected by agar-overlay high-performance thinlayer chromatography-bioautography assay followed by liquid chromatography-diode array detection-mass spectrometry analysis.Results:A total of 28 compounds were detected in two extracts of R.crispus and 26 compounds in four different R.sanguineus extracts,with catechin as a dominant component.Anti-A.baumannii activity was confirmed for all six R.sanguineus and R.crispus extracts at the concentration range from 1 to 4 mg/mL.Neither examined single compounds nor their binary combinations exhibited an anti-A.baumannii activity(MIC>256μg/mL).The bioautography showed that fractions with the most prominent anti-A.baumannii activity tended to contain more polar compounds,predominantly flavonol(quercetin and kaempherol)glycosides;but also fractions containing flavanone(eriodictyol)glycosides and anthraquinone(emodin)glycosides;and less polar eriodictyol aglycone.Conclusions:The results justify and elucidate the traditional application of R.sanguineus and R.crispus extracts for wound healing,indicating the necessity for their further examination in combat against multidrug-resistant A.baumannii isolates from wounds.

Antibacterial activities of selected medicinal plants in traditional treatment of human wounds in Ethiopia

Objective:To evaluate the activity of selected Ethiopian medicinal plants traditionally used for wound treatment against wound-causing bacteria.Methods:Samples of medicinal plants(Achyranthes aspera,Brucea antidysenteriea,Datura stramonium,Croton macrostachyus,Acokanthera xchimperi.,Phytolacca dodecandra,Milhttia ferruginea,and Solanum incanum)were extracted using absolute methanol and water and tested for their antimicrobial activities against clinical isolates and standard strains of wound-causing bacteria using agar well diffusion and micro titer plate methods.Results:Most of the plant extracts had antibacterial activities,among which Acokanthera schimperi and Brucea antidysenteriea inhibited growth of 100%and 35%of the test organisms,respectively.Methanolic extracts had higher activities compared with their corresponding aqueous extracts.The most susceptible organism to the extracts was Streptococcus pyogens while the most resistant were Escherichia coli and Proteus vulgaris.Conclusions:This finding justifies the use of the plants in wound healing and their potential activity against woundcausing bacteria.Their toxicity level and antimicrobial activity with different extraction solvents should further be studied to use them as sources and templates for the synthesis of drugs to control wound and other disease-causing bacteria.

老年高血压患者拔牙前后血压水平与创口愈合效果的关系

目的 探讨老年高血压患者拔牙前后血压水平与创口愈合效果的关系。方法 选取2019年1月至2021年12月北京市普仁医院收治的115例老年高血压患者为研究对象,所有患者行拔牙治疗。统计患者拔牙后1周创口愈合情况,并依据创口愈合情况分为愈合不良组、愈合良好组。比较两组临床资料及拔牙前后收缩压及舒张压差值;分析老年高血压患者创口愈合效果的影响因素;评估拔牙前后血压水平对创口愈合效果的预测价值。结果 115例老年高血压患者拔牙后创口愈合良好94例,创口愈合不良21例。愈合不良组血清白细胞介素-6(IL-6)水平,拔牙前后收缩压、舒张压差值高于愈合良好组(P<0.05)。IL-6、拔牙前后收缩压、舒张压差值为老年高血压患者创口愈合不良的危险因素(OR=3.080、2.954、3.350,P<0.05)。拔牙前后收缩压、舒张压差值联合检测的曲线下面积高于各项单独预测(P<0.05)。结论 老年高血压患者拔牙前后血压水平与创口愈合效果相关,且拔牙前后收缩压、舒张压差值联合检测对老年高血压患者创口愈合效果的预测效能较高。

炭药提取液对小鼠皮肤伤口愈合过程中胶原组成的影响

研究炭药提取液(液体敷料1与液体敷料2)对小鼠皮肤伤口愈合过程中Ⅰ、Ⅲ胶原蛋白含量变化的影响。以小鼠全皮层创伤为模型,将液体敷料1、液体敷料2涂抹于小鼠背部伤口,给药3、6、9、13、17和21 d后,通过液质联用法测定小鼠创面组织中Ⅰ、Ⅲ型胶原含量。结果表明:液体敷料1与液体敷料2能明显提高创面愈合率并加速结痂掉落,并在第9、13天明显增加总胶原的含量(P<0.01);第3、9天,液体敷料1与液体敷料2组的Ⅰ、Ⅲ型胶原含量均大于空白组(P<0.05);且在第17、21天,两组胶原Ⅰ/Ⅲ的比例均大于空白组(P<0.01)。研究为探索炭药提取液促进伤口愈合的机理提供基础。

负载石榴皮提取物的聚乙烯醇基水凝胶伤口敷料的制备与表征

以聚乙烯醇(PVA)和羧甲基纤维素钠(Na-CMC)为原料,以丙三醇(GL)水溶液为溶剂,利用循环冻融法制备一种负载石榴皮提取物(PPE)的水凝胶。对水凝胶的化学结构、透光率、抗干燥性、溶胀性、拉伸性能、体外药物释放、抗氧化性及抗菌性进行了研究。结果表明,制备的水凝胶具备较好的抗干燥性,室温下放置72 h仍能保持完整。PPE的加入使水凝胶的透光率降低,溶胀性、拉伸性提高,同时使水凝胶具备良好的抗菌性和抗氧化性。经测试水凝胶中PPE的累计释放率最高可达(67.47±3.75)%,对金黄色葡萄球菌、大肠杆菌均具有较好的抑菌性,抑菌圈最大分别为(18.78±0.26)mm、(15.11±0.23)mm,对1,1-二苯基-2-苦肼基(DPPH)的清除率最高可达(93.65±2.27)%。

创面分泌液对表皮干细胞体外趋化作用的实验研究

目的观察创面分泌液对体外培养表皮干细胞(ESCs)的趋化作用,并初步探索其作用机制。方法细胞免疫荧光化学、流式细胞仪技术检测表皮干细胞表达趋化因子受体CXCR4的情况;采用Transwell培养体系,观察创面分泌液对ESCs的趋化作用。结果细胞免疫荧光化学结果显示,表皮干细胞胞膜及胞浆内FITC染色呈阳性表达;流式细胞仪检测结果,表皮干细胞表达CXCR4阳性率为43%;体外趋化试验结果显示,创面分泌液组从Transwell培养体系微孔膜上层迁移至下层的细胞数约为87±9.12,明显多于受体拮抗组21±2.71和K-SFM对照组15±3.28(P<0.05);受体拮抗组迁移细胞数虽稍多于K-SFM对照组,但差异无统计学意义(P>0.05)。结论本实验通过Transwell培养体系初步验证了人创面分泌液对体外培养的表皮干细胞具有趋化作用,检测到人表皮干细胞表达趋化因子受体CXCR4,因此推测创面分泌液中可能含有CXCR4的惟一配体SDF-1,并通过SDF-1/CXCR4介导完成对表皮干细胞的趋化效应。

脑创伤组织提取液对培养星形胶质细胞的形态特征及FOS基因表达的影响

本研究应用分子杂交和形态学方法观察脑创伤组织提取液对培养大鼠脑星形胶质细胞ｃ－ｆｏｓ原癌基因表达的影响及形态特征改变。发现在分散培养７ｄ后换置于含有脑创伤组织提取液的培养液３０～６０ｍｉｎ，斑点杂交显示星形胶质细胞ｃ－ｆｏｓ原癌基因表达比加正常脑组织提取液组明显，２ｈ后表达减弱．统计学表明有显著差异．推测损伤因素引起了ｃ－ｆｏｓ原癌基因表达．当将脑创伤组织提取液加入新生鼠星形胶质细胞培养液３ｄ后，体视学观测其细胞数无明显增加．以上结果提示损伤因素诱导星形胶质细胞内表达ＦＯＳ蛋白可能刺激反应性胶质增生．