Diabetic wounds are a difficult medical challenge.Excessive secretion of matrix metalloproteinase-9(MMP-9)in diabetic wounds further degrades the extracellular matrix and growth factors and causes severe vascular dama...Diabetic wounds are a difficult medical challenge.Excessive secretion of matrix metalloproteinase-9(MMP-9)in diabetic wounds further degrades the extracellular matrix and growth factors and causes severe vascular damage,which seriously hinders diabetic wound healing.To solve these issues,a double-network porous hydrogel composed of poly(methyl methacrylate-co-acrylamide)(p(MMA-co-AM))and polyvinyl alcohol(PVA)was constructed by the high internal phase emulsion(HIPE)technique for the delivery of potassium sucrose octasulfate(PSO),a drug that can inhibit MMPs,increase angiogenesis and improve microcirculation.The hydrogel possessed a typical polyHIPE hierarchical microstructure with interconnected porous morphologies,high porosity,high specific surface area,excellent mechanical properties and suitable swelling properties.Meanwhile,the p(MMA-co-AM)/PVA@PSO hydrogel showed high drug-loading performance and effective PSO release.In addition,both in vitro and in vivo studies showed that the p(MMA-co-AM)/PVA@PSO hydrogel had good biocompatibility and significantly accelerated diabetic wound healing by inhibiting excessive MMP-9 in diabetic wounds,increasing growth factor secretion,improving vascularization,increasing collagen deposition and promoting re-epithelialization.Therefore,this study provided a reliable therapeutic strategy for diabetic wound healing,some theoretical basis and new insights for the rational design and preparation of wound hydrogel dressings with high porosity,high drug-loading performance and excellent mechanical properties.展开更多
Our feet are often subjected to moist and warm environments,which can promote the growth of harmful bacteria and the development of severe infection in wounds located in the foot.As a result,there is a need for new an...Our feet are often subjected to moist and warm environments,which can promote the growth of harmful bacteria and the development of severe infection in wounds located in the foot.As a result,there is a need for new and innovative strategies to safely sterilize feet,when shoes are worn,to prevent any potential foot-related diseases.In this paper,we have produced a non-destructive,biocompatible and convenient-to-use insole by embedding a BaTiO3(BT)ferroelectric material into a conventional polydimethylsilane(PDMS)insole material to exploit a ferroelectric catalytic effect to promote the antibacterial and healing of infected wounds via the ferroelectric charges generated during walking.The formation of reactive oxygen species generated through a ferroelectric catalytic effect in the PDMS-BT composite is shown to increase the oxidative stress on bacteria and decrease both the activity of bacteria and the rate of formation of bacterial biofilms.In addition,the ferroelectric field generated by the PDMS-BT insole can enhance the level of transforming growth factor-beta and CD31 by influencing the endogenous electric field of a wound,thereby promoting the proliferation,differentiation of fibroblasts and angiogenesis.This work therefore provides a new route for antimicrobial and tissue reconstruction by integrating a ferroelectric biomaterial into a shoe insole,with significant potential for health-related applications.展开更多
Microneedle, as a novel drug delivery system, has attracted widespread attention due to its non-invasiveness, painless and simple administration, controllable drug delivery, and diverse cargo loading capacity. Althoug...Microneedle, as a novel drug delivery system, has attracted widespread attention due to its non-invasiveness, painless and simple administration, controllable drug delivery, and diverse cargo loading capacity. Although microneedles are initially designed to penetrate stratum corneum of skin for transdermal drug delivery, they, recently, have been used to promote wound healing and regeneration of diverse tissues and organs and the results are promising. Despite there are reviews about microneedles, few of them focus on wound healing and tissue regeneration. Here, we review the recent advances of microneedles in this field. We first give an overview of microneedle system in terms of its potential cargos (e.g., small molecules, macromolecules, nucleic acids, nanoparticles, extracellular vesicle, cells), structural designs (e.g., multidrug structures, adhesive structures), material selection, and drug release mechanisms. Then we briefly summarize different microneedle fabrication methods, including their advantages and limitations. We finally summarize the recent progress of microneedle-assisted wound healing and tissue regeneration (e.g., skin, cardiac, bone, tendon, ocular, vascular, oral, hair, spinal cord, and uterine tissues). We expect that our article would serve as a guideline for readers to design their microneedle systems according to different applications, including material selection, drug selection, and structure design, for achieving better healing and regeneration efficacy.展开更多
It is of great importance to treat a bacterial-infected wound by a smart dressing capable of delivering antibiotics in a smart manner without causing drug resistance.The construction of smart release nanocontainers re...It is of great importance to treat a bacterial-infected wound by a smart dressing capable of delivering antibiotics in a smart manner without causing drug resistance.The construction of smart release nanocontainers responsive to near-infrared(NIR)laser irradiation in an on-demand and stepwise way is a promising strategy for avoiding the emergence of multidrug-resistant bacteria.Here,we develop a hydrogel composite made of alginate and nanotubes with an efficient NIR-triggered release of rifampicin and outstanding antibacterial ability.This composite hydrogel is prepared through co-encapsulating antibacterial drug(rifampicin),NIR-absorbing dye(indocyanine green),and phase-change materials(a eutectic mixture of fatty acids)into halloysite nanotubes,followed by incorporation into alginate hydrogels,allowing the in-situ gelation at room temperature and maintaining the integrity of drug-loaded nanotubes.Among them,the eutectic mixture with a melting point of 39℃ serves as the biocompatible phase-change material to facilitate the NIR-triggered drug release.The resultant phase-change material gated-nanotubes exhibit a prominent photothermal efficiency with multistep drug release under laser irradiation.In an in vitro assay,composite hydrogel provides good antibacterial potency against Staphylococcus aureus,one of the most prevalent microorganisms of dangerous gas gangrene.A bacterial-infected rat full-thickness wound model demonstrates that the NIR-responsive composite hydrogel inhibits the bacteria colonization and suppresses the inflammatory response caused by bacteria,promoting angiogenesis and collagen deposition to accelerate wound regeneration.The NIR-responsive composite hydrogel has a great po-tential as an antibacterial wound dressing functionalized with controlled multistep treatment of the infected sites.展开更多
The complication of diabetes,which is known as diabetic foot ulcer(DFU),is a significant concern due to its association with high rates of disability and mortality.It not only severely affects patients’quality of lif...The complication of diabetes,which is known as diabetic foot ulcer(DFU),is a significant concern due to its association with high rates of disability and mortality.It not only severely affects patients’quality of life,but also imposes a substantial burden on the healthcare system.In spite of efforts made in clinical practice,treating DFU remains a challenging task.While mesenchymal stem cell(MSC)therapy has been extensively studied in treating DFU,the current efficacy of DFU healing using this method is still inadequate.However,in recent years,several MSCs-based drug delivery systems have emerged,which have shown to increase the efficacy of MSC therapy,especially in treating DFU.This review summarized the application of diverse MSCs-based drug delivery systems in treating DFU and suggested potential prospects for the future research.展开更多
Background:Wound healing is a process that requires angiogenesis and antibacterial activities and it remains a challenge for both experimental and clinical research worldwide.Zn2+has been reported to be widely involve...Background:Wound healing is a process that requires angiogenesis and antibacterial activities and it remains a challenge for both experimental and clinical research worldwide.Zn2+has been reported to be widely involved in angiogenesis and exerts antibacterial effects,making it suitable as a treatment to promote wound healing.Therefore Zn2+-loaded adhesive bacterial cellulose hydrogel was designed to observe its angiogenic and antibacterial abilities in the wound healing process.Methods:The characterization,tensile strength,swelling behaviors and antibacterial activity of bacterial cellulose/polydopamine/zeolitic imidazolate framework-8(BC/PDA/ZIF8)hydrogels were tested.Cell-Counting-Kit-8(CCK8),transwell,tube formation and real time qunantitative PCR(qRT-PCR)assays were performed to evaluate the cell compatibility of BC/PDA/ZIF8 hydrogels in vitro.A full-thickness defect wound model and histological assays were used to evaluate the BC/PDA/ZIF8 hydrogels in vivo.Results:The prepared BC/PDA/ZIF8 hydrogels exhibited suitable mechanical strength,excellent swelling properties,good tissue adhesion,efficient angiogenic and antibacterial effects and good performance as a physical barrier.In vivo experiments showed that the BC/PDA/ZIF8 hydrogels accelerated wound healing in a full-thickness defect wound model by stimulating angiogenesis.Conclusions:This study proved that BC/PDA/ZIF8 hydrogels possess great potential for promoting satisfactory wound healing in full-thickness wound defects through antibacterial effects and improved cell proliferation,tissue formation,remodeling and re-epithelialization.展开更多
基金supported by the National Key Research and Development Program of China(2022YFB4601402)the National Natural Science Foundation of China(32201109,51772233,82072446)+3 种基金the Guangdong Basic and Applied Basic Research Foundation(2022B1515120052,2021A1515110557)the Key Basic Research Program of Shenzhen(JCYJ20200109150218836)the Chaozhou Branch of Chemistry and Chemical Engineering Guangdong Laboratory(HJL202202A002)the Trauma Microsurgery Clinical Medical Research Center of Hubei Province and the Health Commission of Hubei Province Medical Leading Talent Project(LJ20200405).
文摘Diabetic wounds are a difficult medical challenge.Excessive secretion of matrix metalloproteinase-9(MMP-9)in diabetic wounds further degrades the extracellular matrix and growth factors and causes severe vascular damage,which seriously hinders diabetic wound healing.To solve these issues,a double-network porous hydrogel composed of poly(methyl methacrylate-co-acrylamide)(p(MMA-co-AM))and polyvinyl alcohol(PVA)was constructed by the high internal phase emulsion(HIPE)technique for the delivery of potassium sucrose octasulfate(PSO),a drug that can inhibit MMPs,increase angiogenesis and improve microcirculation.The hydrogel possessed a typical polyHIPE hierarchical microstructure with interconnected porous morphologies,high porosity,high specific surface area,excellent mechanical properties and suitable swelling properties.Meanwhile,the p(MMA-co-AM)/PVA@PSO hydrogel showed high drug-loading performance and effective PSO release.In addition,both in vitro and in vivo studies showed that the p(MMA-co-AM)/PVA@PSO hydrogel had good biocompatibility and significantly accelerated diabetic wound healing by inhibiting excessive MMP-9 in diabetic wounds,increasing growth factor secretion,improving vascularization,increasing collagen deposition and promoting re-epithelialization.Therefore,this study provided a reliable therapeutic strategy for diabetic wound healing,some theoretical basis and new insights for the rational design and preparation of wound hydrogel dressings with high porosity,high drug-loading performance and excellent mechanical properties.
基金National Natural Science Foundation of China,Grant/Award Numbers:52172265,5230130435Scientific research project of Hunan Provincial Department of Education,Grant/Award Number:21B0009+2 种基金Hunan Excellent Youth Science Foundation,Grant/Award Number:2022JJ20067State Key Laboratory of Powder Metallurgy,Central South University,Changsha,Chinathe Hong Kong Polytechnic University,Grant/Award Number:1-W34B。
文摘Our feet are often subjected to moist and warm environments,which can promote the growth of harmful bacteria and the development of severe infection in wounds located in the foot.As a result,there is a need for new and innovative strategies to safely sterilize feet,when shoes are worn,to prevent any potential foot-related diseases.In this paper,we have produced a non-destructive,biocompatible and convenient-to-use insole by embedding a BaTiO3(BT)ferroelectric material into a conventional polydimethylsilane(PDMS)insole material to exploit a ferroelectric catalytic effect to promote the antibacterial and healing of infected wounds via the ferroelectric charges generated during walking.The formation of reactive oxygen species generated through a ferroelectric catalytic effect in the PDMS-BT composite is shown to increase the oxidative stress on bacteria and decrease both the activity of bacteria and the rate of formation of bacterial biofilms.In addition,the ferroelectric field generated by the PDMS-BT insole can enhance the level of transforming growth factor-beta and CD31 by influencing the endogenous electric field of a wound,thereby promoting the proliferation,differentiation of fibroblasts and angiogenesis.This work therefore provides a new route for antimicrobial and tissue reconstruction by integrating a ferroelectric biomaterial into a shoe insole,with significant potential for health-related applications.
基金supported by the grant from the Guangdong Basic and Applied Basic Research Foundation(2020B1515130002)Collaborative Research with World-leading Research Groups from The Hong Kong Polytechnic University(P0039523)+2 种基金National Excellent Young Scientists Fund(Hong Kong and Macao),National Natural Science Foundation of China(No.82122002)National Natural Science Foundation of China(No.U1909218)the Science Fund for Creative Research Groups of the National Natural Science Foundation of China(No.T2121004).
文摘Microneedle, as a novel drug delivery system, has attracted widespread attention due to its non-invasiveness, painless and simple administration, controllable drug delivery, and diverse cargo loading capacity. Although microneedles are initially designed to penetrate stratum corneum of skin for transdermal drug delivery, they, recently, have been used to promote wound healing and regeneration of diverse tissues and organs and the results are promising. Despite there are reviews about microneedles, few of them focus on wound healing and tissue regeneration. Here, we review the recent advances of microneedles in this field. We first give an overview of microneedle system in terms of its potential cargos (e.g., small molecules, macromolecules, nucleic acids, nanoparticles, extracellular vesicle, cells), structural designs (e.g., multidrug structures, adhesive structures), material selection, and drug release mechanisms. Then we briefly summarize different microneedle fabrication methods, including their advantages and limitations. We finally summarize the recent progress of microneedle-assisted wound healing and tissue regeneration (e.g., skin, cardiac, bone, tendon, ocular, vascular, oral, hair, spinal cord, and uterine tissues). We expect that our article would serve as a guideline for readers to design their microneedle systems according to different applications, including material selection, drug selection, and structure design, for achieving better healing and regeneration efficacy.
基金National Natural Science Foundation of China(Grant No.82002049 and 52073014,to J.X.)Key Program of Beijing Natural Science Foundation(Z200025)+1 种基金Fundamental Research Funds for the Central Universities(buctrc202020)YL thanks support by T.Pipes Nano Microsystem Endowment,Louisiana Tech University,USA.
文摘It is of great importance to treat a bacterial-infected wound by a smart dressing capable of delivering antibiotics in a smart manner without causing drug resistance.The construction of smart release nanocontainers responsive to near-infrared(NIR)laser irradiation in an on-demand and stepwise way is a promising strategy for avoiding the emergence of multidrug-resistant bacteria.Here,we develop a hydrogel composite made of alginate and nanotubes with an efficient NIR-triggered release of rifampicin and outstanding antibacterial ability.This composite hydrogel is prepared through co-encapsulating antibacterial drug(rifampicin),NIR-absorbing dye(indocyanine green),and phase-change materials(a eutectic mixture of fatty acids)into halloysite nanotubes,followed by incorporation into alginate hydrogels,allowing the in-situ gelation at room temperature and maintaining the integrity of drug-loaded nanotubes.Among them,the eutectic mixture with a melting point of 39℃ serves as the biocompatible phase-change material to facilitate the NIR-triggered drug release.The resultant phase-change material gated-nanotubes exhibit a prominent photothermal efficiency with multistep drug release under laser irradiation.In an in vitro assay,composite hydrogel provides good antibacterial potency against Staphylococcus aureus,one of the most prevalent microorganisms of dangerous gas gangrene.A bacterial-infected rat full-thickness wound model demonstrates that the NIR-responsive composite hydrogel inhibits the bacteria colonization and suppresses the inflammatory response caused by bacteria,promoting angiogenesis and collagen deposition to accelerate wound regeneration.The NIR-responsive composite hydrogel has a great po-tential as an antibacterial wound dressing functionalized with controlled multistep treatment of the infected sites.
基金Supported by Science and Health Joint Medical Research Project of Chongqing,No.2022MSXM133Natural Science Foundation of Chongqing,No.CSTB2022NSCQ-MSX1522,No.CSTB2023NSCQ-MSX0246,No.CSTB2022NSCQ-MSX1271+1 种基金The First Batch of Key Disciplines on Public Health in Chongqing and ScienceHealth Joint Project of Dazu District Science and Technology Bureau,No.DZKJ,2022CCC1001.
文摘The complication of diabetes,which is known as diabetic foot ulcer(DFU),is a significant concern due to its association with high rates of disability and mortality.It not only severely affects patients’quality of life,but also imposes a substantial burden on the healthcare system.In spite of efforts made in clinical practice,treating DFU remains a challenging task.While mesenchymal stem cell(MSC)therapy has been extensively studied in treating DFU,the current efficacy of DFU healing using this method is still inadequate.However,in recent years,several MSCs-based drug delivery systems have emerged,which have shown to increase the efficacy of MSC therapy,especially in treating DFU.This review summarized the application of diverse MSCs-based drug delivery systems in treating DFU and suggested potential prospects for the future research.
基金supported by the National Natural Science Foundation of China(52003048)China Postdoctoral Science Foundation(2021 T140110).
文摘Background:Wound healing is a process that requires angiogenesis and antibacterial activities and it remains a challenge for both experimental and clinical research worldwide.Zn2+has been reported to be widely involved in angiogenesis and exerts antibacterial effects,making it suitable as a treatment to promote wound healing.Therefore Zn2+-loaded adhesive bacterial cellulose hydrogel was designed to observe its angiogenic and antibacterial abilities in the wound healing process.Methods:The characterization,tensile strength,swelling behaviors and antibacterial activity of bacterial cellulose/polydopamine/zeolitic imidazolate framework-8(BC/PDA/ZIF8)hydrogels were tested.Cell-Counting-Kit-8(CCK8),transwell,tube formation and real time qunantitative PCR(qRT-PCR)assays were performed to evaluate the cell compatibility of BC/PDA/ZIF8 hydrogels in vitro.A full-thickness defect wound model and histological assays were used to evaluate the BC/PDA/ZIF8 hydrogels in vivo.Results:The prepared BC/PDA/ZIF8 hydrogels exhibited suitable mechanical strength,excellent swelling properties,good tissue adhesion,efficient angiogenic and antibacterial effects and good performance as a physical barrier.In vivo experiments showed that the BC/PDA/ZIF8 hydrogels accelerated wound healing in a full-thickness defect wound model by stimulating angiogenesis.Conclusions:This study proved that BC/PDA/ZIF8 hydrogels possess great potential for promoting satisfactory wound healing in full-thickness wound defects through antibacterial effects and improved cell proliferation,tissue formation,remodeling and re-epithelialization.
