Garnet and Zircon U-Pb Geochronology and Geochemistry Reveal Genesis of the Dafang Au-Pb-Zn-Ag Deposit,Southern Hunan

Garnet is a primary mineral in skarn deposits and plays a significant role in recording copious mineralization and metallogenic information.This study systematically investigates the geochemistry and geochronology of garnet and zircon in the Dafang Au-Pb-Zn-Ag deposit,which represents prominent gold mineralization in southern Hunan,China.Garnet samples with distinct zoning patterns and compositional variations were identified using various analytical techniques,including Backscattered Electron(BSE)imaging,Cathodoluminescence(CL)response,textural characterization,and analysis of rare-earth elements(REE),major contents,and trace element compositions.The garnet was dated U-Pb dating,which yielded a lower intercept age of 161.06±1.93 Ma.This age is older than the underlying granodiorite porphyry,which has a concordia age of 155.13±0.95 Ma determined by zircon U-Pb dating.These results suggest that the gold mineralization may be related to the concealed granite.Two groups of garnet changed from depleted Al garnet to enriched Al garnet,and the rare earth element(REE)patterns of these groups were converted from light REE(LREE)-enriched and heavy REE(HREE)-depleted with positive europium(Eu)anomalies to medium REE(MREE)-enriched from core to rim zoning.The different REE patterns of garnet in various zones may be attributed to changes in the fluid environment and late superposition alteration.The development of distal skarn in the southern Hunan could be a significant indicator for identifying gold mineralization.

Innovative Research Group of Hunan Province

Team Profile Focusing on the major needs of national ecological civilization construction and soil environmentalprotection,the team systematically carried out researches on mining&metallurgy sites remediation,and the soilformation of bauxite residue.A new model of physical-chemical-biological based ecological disposal for bauxiteresidue was proposed,and a replicable and popularized soil-groundwater collaborative remediation technologyfor smelting sites was formed,with the demonstration projects of 250000 m^(2).

Material Analysis of Traditional Folk Dwellings and Its Modern Inheritance:A Case Study of Traditional Folk Dwellings in Hunan Province

Traditional folk dwellings contain rich cultural connotations and plain architectural techniques.In architecture,material is the most fundamental thing,different materials can demonstrate different architectural forms,and reflect local characteristics and change of the time.Therefore,it is of great significance to explore the material selection of traditional Chinese folk dwellings.The paper took traditional folk dwellings in Hunan for example to analyze the regional materials and construction of these dwellings,discussed the application of traditional materials in modern architecture,used some cases to explore the innovative application of traditional materials,so as to figure out the new direction of applying traditional materials,and provide references for the construction of modern architecture.

Origin of the Yueguang gold deposit in Xinhua, Hunan Province, South China: insights from fl uid inclusion and hydrogen–oxygen stable isotope analysis

The Yueguang gold deposit is located in Fengjia,Xinhua County,Hunan Province,South China.It represents a recently discovered small-scale gold deposit situated in the southwestern region of the Jiangnan Orogenic Belt,west of the Baimashan granitic batholith.In order to discern the characteristics of the ore-formingfluids,the underlying mineralization processes,and establish a foundation for the origin of the Yueguang gold depositfluid inclusion micro-thermometry,as well as quartz hydrogen and oxygen isotope analysis,have been carried out on samples obtained from various stages of mineralization.The hydrothermal miner-alization stages within the Yueguang gold deposit can be categorized into three stages:(i)the barren,pre-ore quartz-pyrite stage(Stage Ⅰ),the quartz-pyrite-gold stage(Stage Ⅱ),and the post-ore quartz-carbonate stage(Stage Ⅲ),with the second stage being the main mineralization stage.Thefluid inclusions identified in samples from the main min-eralization stage can predominantly be described with the NaCl–H_(2)O and CO_(2)–NaCl–H_(2)O systems.These inclusions display homogenization temperatures ranging from 158.8 to 334.9℃,and thefluid salinity ranges from 0.3%to 4.0%(wt.%NaCl equiv.).Laser Raman spectroscopy analysis of individual inclusions further reveals the presence of gas-phases such as CO_(2),CH_(4),and N_(2).Isotopic analysis indicatesδ^(18)Ofluid values ranging from 3.95 to 6.7‰ and δDH_(2)O values ranging from-71.9 to-55.7‰.These results indi-cate that the ore-formingfluid of the Yueguang gold deposit belongs to metamorphic hydrothermalfluids of middle-low temperature and low salinity.In the process of ore formation,gold is transported in the form of Au(HS)2-complexes,with gold deposition being driven byfluid immiscibility.Therefore,the Yueguang gold deposit is categorized as an orogenic gold deposit dominated by metamorphic hydrother-malfluid.It may become a new target for gold exploration in the Baimashan region,central Hunan Province.

Re–Os and Pb isotope features of pyrite in the Shihangli graphite deposit:implications of coal-generated graphite mineralization in central Hunan,South China

The coal metamorphism in Central Hunan pro-vides valuable information about hydrothermal activity and water/rock reactions.Learning how to collect age data on hydrothermal fluid systems is necessary for understanding the history and genetic mechanisms of large-scale coal-generated graphite deposits.The Shihangli graphite deposit,formed by significant siliceous hydrothermal alteration,is the most distinctive in Central Hunan.Re–Os dating of pyrite from the Shihangli graphite deposit demonstrates that the coal-generated graphite mineraliza-tion age is-127.6±3.8 Ma.Based on in-situ mineral analysis,the hydrothermal pyrite in the Shihangli graphite deposit is mostly enriched in Sb,As,Au,W,Ag,Cu,Pb,and Zn.Based on the pyrite Re–Os isochron,the initial(^(187)Os/^(188)Os)values of pyrite were 1.03±0.24 and the Os(t)values varied from 571.8 to 755.1.Pyrite from the Shihangli graphite deposit comprises a Pb isotope composition similar to that of the Madiyi Formation bulk rock and stibnite from the Xikuangshan Sb deposit.Based on the Re–Os,Sr,S,and Pb isotopic compositions of sul-fides in the graphite and Sb deposits in Central Hunan,the Madiyi Formation was likely the primary source of ore-forming elements(Sb,Au,and As).The Re–Os and Pb isotope compositions of pyrite most likely reflect when large-scale fluid migration and coal-generated graphite mineralization occurred in Central Hunan.

The Late Tremadocian(Ordovician)Graptolite Kiaerograptus from Central Hunan,China:Taxonomy and Biostratigraphy

Well-preserved graptolites of the genus Kiaerograptus are first reported from the upper Tremadocian Yinchupu Formation in the Nanba section,Yiyang,Hunan Province,South China,including four species,i.e.,Kiaerograptus lauzonensis(Erdtmann,1966),Kiaerograptus stoermeri(Erdtmann,1965),Kiaerograptus cf.supremus?Lindholm,1991,and Kiaerograptus sp.,which are robust in form.Based on these new specimens,the Kiaerograptus biozone is established for the first time in South China,increasing the late Tremadoc graptolite biozones in South China from four to five,in ascending order as follows:the Adelograptus tenellus biozone,the Aorograptus victoriae biozone,the Kiaerograptus biozone,the Sagenograptus murrayi biozone,and the Hunnegraptus copiosus biozone.A review of the occurrence records for robust Kiaerograptus species worldwide reveals that the distribution is restricted to the Aorograptus victoriae biozone and Kiaerograptus biozone in the late Tremadocian.This limited stratigraphical distribution makes Kiaerograptus a valuable taxon for precise biostratigraphical correlation at both the regional and global scales.

The Pathogenic Characteristics of the Initial Three Mpox Cases in Hunan Province, China

Mpox virus (MPXV) belongs to the family Poxviridae,subfamily Chordopoxvirinae,and genus Orthopoxvirus,which also includes Variola virus(VARV),Cowpox virus (CPXV),and Vaccinia virus(VACV).Clinical symptoms of mpox are similar to smallpox but are relatively milder^([1]).MPXV was first isolated from crab-eating monkeys in the tropical rainforests of Africa in 1958^([2]).The first human case of MPXV infection was reported in the Congo in1970^([3]).Following the eradication of smallpox in1977,mpox has emerged as one of the prominent viral members of the Poxviridae family,significantly impacting public health^([4]),mainly prevalent in Central and West Africa.Starting from May 2022.

Study on the Change Character of Double Rice Cold Damage in Hunan and Hubei Province during the Past 50 Years

Based on the meteorological data of representative meteorological stations in Hunan and Hubei from 1961 to 2008 years,the change character of double rice cold damage under the background of climate change were analyzed adopting 30 years glide accumulation method.The analysis showed that the 30 years sliding cumulative days of mild low-temperature during spring sowing in Hunan and Hubei were decreased in the recent 50 years,as well as the 30 years sliding cumulative days of moderate low-temperature in central south Hubei,northern and western Hunan.However,there was an increase in southern Hunan,so was the 30 years sliding cumulative days of severe low-temperature.The 30 years sliding cumulative days of mild low-temperature in May reduced in Hunan and Hubei.Instead,that of moderate low-temperature in May in central south Hubei was on the rise,and it was opposite in northern and southern Hunan,but there was no obvious change in western Hunan.The 30 years sliding cumulative days of severe low-temperature showed a decreasing tendency in May in central south Hubei,northern and western Hunan,and southern Hunan showed a contrary tendency.The 30 years sliding cumulative days of mild cold dew wind in Hunan and Hubei were decreased,that of moderate cold dew wind in central south Hubei,northern and western Hunan had the same tendency,but there was a contrary tendency in southern Hunan.The 30 years sliding cumulative days of severe cold dew wind was decreased in central south Hubei and increased in western Hunan,without cold dew wind in northern and southern Hunan.

Analysis of Emergy Structure,Function and Efficiency of Agro-ecosystem in Hunan Province

[Objective] The aim was to explore function and efficiency of emergy input/ output of Hunan agricultural ecosystem to reveal relationship of human being with natural resources and environment. [Method] Emergy structure, function and efficien- cy of Hunan agricultural ecosystem were analyzed based on input/output data in Hunan Province in 2009 as per emergy theory. [Result] The structure characters of agricultural ecosystem were as follows： Hunan is characterized with traditional agri- culture, depending greatly on both human and animal labor. For industrial accessory emergy, chemical fertilizers make most contribution to the system, and agricultural mechanization is not satisfied. Furthermore, renewable ratio of industrial accessory emergy is lower, and development and potential of green energy is promising. In addition, prices of the products are lower, without consideration of contribution made by natural resources and environment. It also suggested that Hunan agricultural chain is short and added value of products is not high. In general, emergy output of farming and animal husbandry dominates and the prices are lower than those of forestry and fishery. The function of the system was as follows： With lower environ- mental load ratio, the system is overloaded by population and the index of sustain- able development was 5.96, suggesting that the system enjoys vitality and potential, but the economy is undeveloped and the pressure from natural resources and envi- ronment is not high. The emergy output ratio was a little lower than national level in 2009, suggesting Hunan agricultural production is extensive. [Conclusion] The re- search indicated that rapid increase of population should be controlled; surplus labor should be transferred; agricultural structure and products structure should be further adjusted; agricultural technology should be further developed; agdcultural mechaniza- tion and modernization should be improved.

CREG promote the acquisition of CREG Hunan

China Rare Earth Group(CREG)announced on November 21st that the company issued80,331,800 A-shares to 14 specific targets at a price of 26.1 yuan/share,raising a total of about 2.097billion yuan,and after deducting related expenses,the net raised funds were about 2.081 billion yuan.The Central SOEs Industrial Investment Fund for Rural Area was the single largest subscriber,and was allocated 22,988,500 shares,with an allocation amount of about 600 million yuan,accounting for28.6%of the total fundraising.After the issuance,the fund would become the third largest shareholder of CREG with a shareholding ratio of 2.17%.

Structural deformation, metallogenic epoch and genetic mechanism of the Woxi Au-Sb-W deposit, Western Hunan Province, South China

The remobilization,migration,precipitation,and enrichment of ore-forming elements are closely related to structures.Therefore,detailed regional and ore-field structural analyses are critical for determining the genesis of a mineral deposit.The Jiangnan Orogenic Belt(JOB)is an important gold polymetallic metallogenic belt in South China,which is characterized by multiple periods of gold mineralization in the Paleozoic and Mesozoic.However,the genesis of these gold polymetallic deposits is still not well understood due to a lack of systematic research on the regional geology,ore-controlling structures and metallogenic mechanism.In this study,a detailed structural survey at the surface and in the subsurface tunnels was conducted on the Woxi Au-Sb-W deposit,the genesis of which is relatively controversial among the gold polymetallic deposits in the JOB due to poor structural constraints.In addition,a wolframite U-Pb dating was carried out to further constrain the relationship between structures and mineralization.Based on the results of these studies,together with those from previous studies,it is proposed that the Woxi deposit and surrounding areas likely underwent six periods of regional deformation,which are constrained in time and geodynamic setting.Furthermore,we present a systematic discussion on the roles of ore-controlling structures in the transportation,distribution,and deposition of ore-forming elements and localization of orebodies.According to the wolframite dating results,structural analyses,and previous data,we propose that the Woxi Au-Sb-W deposit was formed in two stages during the Yanshanian:a W(wolframite)-Au mineralization stage at ca.140 Ma and an Au-Sb-W(scheelite)mineralization stage at<130 Ma.These mineralizing events are interpreted to have a tight relationship with tectonic reactivation,and the ore-forming fluids were derived from deep sources,including those of magmatic or metamorphic origins.The Woxi deposit can therefore be classified as an“intracontinental reactivation-type”,and the mineralization is related to lithospheric extension caused by plate retreat,retention,and delamination following the cessation of westward subduction of the Paleo-Pacific Plate beneath the East Asian continent.

Petrogenesis of skarn in Shizhuyuan W-polymetallic deposit, southern Hunan,China:Constraints from petrology,mineralogy and geochemistry

Skarn is the main altered rock type and is of great importance to mineralization and ore-prospecting in the Shizhuyuan area of Hunan province, China. Its features of petrography, mineralogy and geochemistry were studied systematically. The results show that the skarn mainly consists of garnet skarn, secondary wollastonite-garnet skarn, tremolite-clinozoisite skarn, and few wolframine garnet skarn, idocrase-garnet skarn and wollastonite skarn with granoblastic texture, granular sheet crystalloblastic texture, and massive structure, disseminated structure, mesh-vein structure, comb structure, and banded structure. And, it is mainly composed of garnet, fluorite, chlorite, hornblende, epidote, tremolite, plagioclase, biotite, muscovite, plagioclase, quartz, idocrase, and calcite and so on. The chemical components mainly include SiO2, Al2O3, Fe2O3, MgO and CaO, and the trace elements and REEs consist of Li, Be, V, Co, Zn, Ga, Rb, Sr, Y, Ce, Nd, Pb and Bi, etc. And, the obvious fractionation exists between LREE and HREE, and it shows typical features of Nanling ore-forming granite for W？Sn polymetallic deposit. Skarn is derived from the sedimentary rock, such as limestone, mudstone, argillaceous rock, and few pelitic strips. It is affected by both Shetianqiao formation strata and Qianlishan granite during the diagenesis, indicating a strong reduction environment. The occurrence of skarn, whose mutation site is favorable to the mineralization enrichment, is closely related to the mineralization and prospecting.

Optimized Strategy for Layout of Crop Production Areas in Hunan Province

The optimized strategy made a comprehensive consideration of resources, technology, market orientation, production scale, industry basis and layout based on the principle of crop security and farmers’ income increasing, and determined the general planning on layout and structure optimization of future crop production ar-eas, with present crop production, market outlook, future industry development, con-cluding crop production characteristics of the 4 crop regions, and proposing function orientation and highlights.

New Bivalved Arthropods from the Cambrian(Series 3, Drumian Stage) of Western Hunan, South China

We report two new three-dimensionally phosphatized microfossils, Cambrolongispina reticulata gen. et sp. nov. and Cambrolongispina glabra gen. et sp. nov., from the middle Cambrian （Series 3, Drumian Stage） at Wangcun, Yongshun County, western Hunan, South China. They are bivalved arthropods, with thin, pliable, originally chitinous or chitin-calcareous shields （c. 350-517 I1 m long）. The shields are equipped with a pair of antero-dorsal spines. The spines are internally hollow, varying in length from 1/2 of to as long as the shield length in C. reticulata, and uniformly longer than the shield length in C. glabra. The spines of C. reticulata are ornamented with a longitudinal row of conical or blade-shaped denticles along the posterior edge. Cambrolongispina lacks marginal rims, valve lobation and sulci which are diagnostic of the Bradoriida sensu stricto. It also lacks a donblure/duplicature characteristic of phosphatocopids and some bradoriids. It could be related to the Monasteriidae （which may belong to Bradoriida sensu lato）, both characterized by a pair of antero-dorsal spines. Cambrolongispina may have been meiofaunai detritus feeders that lived on or within sediments. The antero-dorsal spines may have been sensory organs to detect nearby predators. The posterior denticles on these spines might have facilitated the sensory function of the spines.

Analysis of Change of Cultivated Land and Grain Production in Hunan Province

[Objective] This paper aimed at exploring the countermeasures for cultivated land protection and food security assurance and providing references for the formulation of policies related to cultivated land and grain production of the whole province.[Methods] Data of population,cultivated land,grain and others in Hunan Province during 1989 to 2008 were collected by county（district）.The changes of cultivated land and grain production of the whole province were analyzed using Geographical Information System（GIS）.[Results] The population was increasing continuously,the total area of cultivated land was reducing as a whole in spite of increasing in one or two year in one or two county,the total grain production and per capita grain yield was fluctuated within a certain range in recent 20 years of the whole province.The reducing of cultivated land was mainly due to the industrialization and urbanization development.The unbalanced quantity and quality of cultivated land,various natural disasters,readjustment of industry structure and inner readjustment in the agriculture,variety improvement and improvement of cultivated technology mainly affected of the grain production.[Conclusions] The protection of the quantity and quality of cultivated land has to be strengthened to ensure food security.It is important to enhance the ability of resisting drought and flood disasters by improving the construction of agriculture establishment in order to insure the productivity of cultivated land,to increase farmer’s enthusiasm for growing grain with favourable food policy,to improve the per unit area yield of grain by variety improvement and to increase grain sown area by enlarging the double-cropping rice sown area.

Emergy analysis of paddy farming in Hunan Province, China： A new perspective on sustainable development of agriculture

The multi-functionality of paddy farming has become a hot issue recently. Paddy farming provides numerous ecosystem services that are crucial to human well-being. However, evaluation of the contribution of paddy farming to human well-being usually focus on its economic value, while its non-market services are usually ignored. Only evaluating the market profits or market relative benefits cannot reflect comprehensively the contribution of paddy farming to people＇s well-being. This will affect people＇s choices for or against paddy farming activities and people＇s opt for invest or not invest in it. A comprehensive evaluation of paddy farming can provide an important reference for the government and society to conserve the multi-functionality of paddy farming and achieve sustainable development. To this end, this paper reports a case evaluation of paddy farming in Hunan, the largest rice producing as well as rice yield province in China, and uses emergy theory to make a comprehensive evaluation for paddy farming. The emergy evaluation results of the paddy ecosystem in Hunan are as follows： in 2010, the input emergy of the paddy ecosystem in Hunan is 2.51E＋22 sej and the output emergy is 6.31E＋22sej. For the input emergy, the part from natural resources is 1.96E＋21 sej and the part from human society is 2.32E＋22sej; for the output emergy, the part from products is 2.22E＋22 sej, the part from impositive externality is 4.16E＋22 sej and the part from negative externality is –7.41E＋20 sej. Taking the non-market outputs into consideration, the gains from the human economic society＇s 1 $ input in paddy farming, emergy sustainability index（ESI） and emergy profit rate are respectively 2.73 $, 3.53 and 151.31%. If the evaluation leave out the non-market output, the three indexes are only 0.96 $,1.24 and 30.67%. The research results show that non-market services of paddy farming contribute significantly to human well-being. Therefore, in order to protect the multi-functionality of paddy farming and achieve the sustainable management,the government should take reasonable measures and make incentive plans.

Analysis of an Abrupt Rainstorm Process in Central Hunan Province

[Objective] The aim is to expound the abrupt rainstorm in the central Hunan Province on May 6 in 2010.[Method] By dint of NCEP 1°×1° reanalysis data,routine observation data,auto-station precipitation and FY-2C satellite data,the large-scale circulation background and physical condition during the large rainstorm period from the night on May 5 to 6 in 2010 were analyzed.The large scale environment,meso-scale characteristics and potential causes for the formation of large precipitation were revealed.By dint of FY-2D satellite and water-vapor cloud image,infrared cloud image,4 black light temperature,the variation characteristics of heavy rainstorm convection system were analyzed.[Result] Under the favorable environment background,the rainstorm was induced by the meso-scale convection system,and it had close relation with the upper-air trough,shear line in the middle and low layer,ground weak and cold air and the torrent in the upper and low latitude.The result of all kinds of physical quantities suggested that the meso-scale rainstorm above the air had distinct characteristics and the heavy precipitation was in the middle Hunan Province.It was caused by meso-system.The dynamic dry belt above the satellite,TBB=-60℃ center and the large value area of the gradient could provide references for the forecast and pre-warning of large rainstorm.[Conclusion] The study accumulated experiences for the forecast and report of rainstorm in Hunan Province.

A New Species of the Genus Tylototriton (Urodela: Salamandridae) from Northeastern Hunan Province, China

We describe a new species from the family Salamandridae from northeastern Hunan Province of China based on morphological and molecular genetic evidences.The new species,Tylototriton liuyangensis,is a member of the T.asperrimus group,and shares a number of similarities with T.wenxianensis and T.broadoridgus.The dorsal surface of the new Tylototriton species is completely black,with nodule-like warts distributed evenly along the lateral margin of dorsal body,and there is no transverse striae between the warts.There are several diagnostic characters of the new species that differ from those of T.wenxianensis and T.broadoridgus as follows：（1）the interorbital space（IOS）is comparatively wide;（2）there is a bony ridge present on the dorsal head surface,running along the upper eyelids;（3）the space between axilla and groin（AGS）is comparatively large,with the mean male AGS approximately equal to 50%of the snout-vent length（SVL）and the mean female AGS being 53%of SVL;and（4）there is a small genital armature on the anterior angle of labial folds present inside the anal fissure of adult males during the breeding season.The result from the molecular phylogenetic analysis of the genus Tylototriton（including the type specimen of the new species）based on the complete DNA sequence of the mitochondrial ND2 gene（1035 bp）indicated that the new species was similar to T.wenxianensis,T.dabienicus,and T.broadoridgus,but formed an independent clade.This result was consistent with the morphological analysis above,supporting that the Liuyang Population represented a distinct species.

Source and location mechanism for lode gold deposits hosted in metamorphic rocks in northeastern Hunan, China

For understanding the source and location mechanism of lode gold deposits hosted in metamorphic rocks in northeastern Hunan, the authors analyzed the REE (rare earth elements) in ores and their host rocks, metallogenic elements in host rocks near and distant from the ore bodies, and characteristics of ore controlling structures, and deduced their genetic implication. Their geochemical features of REE and metallogenic elements suggest that they are formed by mobilization of dispersed metallogenic materials in Lengjiaxi Group of Middle Proterozoic during deformation and metamorphism process, mainly in Wulingian period. From the attributes of ore controlling structures and regularity of location of gold metallization, it is concluded that the location of gold deposits is closely related to reverse shearing. Ore forming fluids are focused on the secondary faults and extension fractures of reverse shear zones of nearly EW strike by stress driven diffusion and seismic pumping.

Biota and Organic Matter in the Late Sinian and EarlyCambrian Black Rock Series of West Hunan andIts Significance for Metallization

Abstract: A great amount of black rock series has been found in the Upper Sinian Members 2 and 4 of the Jinjiadong Formation, middle-upper Liuchapo Formation and the Lower Cambrian Xiaoyanxi Formation in West Hunan, which is associated with periodic sea-level changes. By the studies of relationships between the distribution and development of the biota and the abundance of Au, Ag, U, V, Ni, Mo and Cu in the Upper Sinian and Early Cambrian black rock series in Cili, Dayong, Yuanling, Xupu and Qianyang of West Hunan, central China, it has been revealed that the enrichment of Ag, V, and Mo is related with the development of multi-cell plants and vendotaenides, and that of Cu and Ni is related to flourishing of bacteria and shelly fossils. The black rock series in the study area contains abundant organic matter, among which the siliceous shale contains the highest TOC, amounting to 4.51–13.4%. All the values of the equivalent vitrinite reflectance (medium values) determinated with the IR-spectroscopic method in the area are over 2.65%, indicating that the organic evolution was at an overmature stage. Due to the effect of enhancement of organic maturation by hydrothermal fluids, the Upper Sinian and Lower Cambrian had a reverse organic maturity profile in most areas of West Hunan. The enrichment of Ag, V, Ni, Mo and U is a result of organic absorption, and that of Au might be attributed to the migration by hydrothermal fluids.