The key proposition of this hypothesis is logic behind the order of movements of Hua Tuo’s qigong Wu Qin Xi(Five animal frolics).To date,there were many discussions about connection of the movements of Wu Qin Xi with...The key proposition of this hypothesis is logic behind the order of movements of Hua Tuo’s qigong Wu Qin Xi(Five animal frolics).To date,there were many discussions about connection of the movements of Wu Qin Xi with existing TCM theories and why Hua Tuo made it in that particular way.Some experts are saying that there is no connection but if all stories of Hua Tuo’s abilities and knowledge were half-truth,he wouldn’t let even the order of movements of qigong that he created be just a random order.Hypothesis is exploring different views on Taiji movement direction,Wu Xing and connection between animals in Wu Qin Xi,Lo Shu square and Sun wheel and proposing possible solution to the question“Why Hua Tuo made such order of animals in Wu Qin Xi?”by analyzing and and cross referencing the common ground between theories and bridging the gap the we were left without any written explanation from the master itself.Further progress and confirmation of this hypothesis requires deeper research and cooperation between Qigong expert historians.展开更多
目的探索可被临床医生直接应用的全外显子测序(WES)数据分析平台及方法。方法选择复旦大学附属儿科医院(我院)临床诊断不明疾病的核心家系3例(例1～3)和仅先证者2例(例4和5)行WES分析,采用Wu Xi NextCODE临床测序数据分析系统(简称NextC...目的探索可被临床医生直接应用的全外显子测序(WES)数据分析平台及方法。方法选择复旦大学附属儿科医院(我院)临床诊断不明疾病的核心家系3例(例1～3)和仅先证者2例(例4和5)行WES分析,采用Wu Xi NextCODE临床测序数据分析系统(简称NextCODE平台)进行快速数据分析,并与我院分子诊断中心已建立的高通量数据分析流程(简称常规数据分析流程)进行参与人员及耗时的比较。结果例1～3基于表型相关候选基因联合遗传模式的分析方法,分别检测到FGFR2基因杂合突变、GBE1基因复合杂合突变及TBX1基因杂合突变;例4和5通过表型相关候选基因分析,分别检测到IL10RA基因纯合突变和复合杂合突变。NextCODE平台自动完成3/7个步骤,从输入表型至生成报告,WES数据分析用时30 min以内。常规数据分析流程自动完成1/7个步骤,6个人工完成步骤需要多个专业人员进行数据的筛选及解读,从输入表型至生成报告,熟练的专业人员用时2～8 h。结论 5例临床诊断不明病例通过WES明确了诊断;NextCODE是直接为临床医生所用、简单快速的WES数据分析平台,有助于协助临床医生直接利用高通量测序数据,准确锁定致病突变,提高诊断效率。展开更多
文摘The key proposition of this hypothesis is logic behind the order of movements of Hua Tuo’s qigong Wu Qin Xi(Five animal frolics).To date,there were many discussions about connection of the movements of Wu Qin Xi with existing TCM theories and why Hua Tuo made it in that particular way.Some experts are saying that there is no connection but if all stories of Hua Tuo’s abilities and knowledge were half-truth,he wouldn’t let even the order of movements of qigong that he created be just a random order.Hypothesis is exploring different views on Taiji movement direction,Wu Xing and connection between animals in Wu Qin Xi,Lo Shu square and Sun wheel and proposing possible solution to the question“Why Hua Tuo made such order of animals in Wu Qin Xi?”by analyzing and and cross referencing the common ground between theories and bridging the gap the we were left without any written explanation from the master itself.Further progress and confirmation of this hypothesis requires deeper research and cooperation between Qigong expert historians.
