Wudang cherry ameliorates urate underexcretion and renal dysfunction in hyperuricemic mice

OBJECTIVE To investigate effects of Wudang cherry on urate excretion and renal function and examined whether renal organic ion transporters were involved in potassium oxonateinduced hyperuricemic mice.METHODS The model of hyperuricemic mice was induced by intraperitoneal injection of potassium oxonate(250 mg·kg^(-1))for 7 d.Water extracts of Wudang cherry at 500 mg·kg^(-1)were orally administered to hyperuricemic mice for 7 d,benzbromarone(20 mg·kg^(-1))and allopurinol(20 mg·kg^(-1))were given as positive controls,vehicle control group was given equal normal saline.Serum and urine levels of uric acid were measured in hyperuricemic and normal mice.Simultaneously,the m RNA and protein levels of mouse urate transporter 1(m URAT1),glucose transporter 9(mGLUT9),organic anion transporters(mOAT1 and mOAT3),ATP-binding cassette,subfamily G,membrane 2(mABCG2)and organic cation/carnitine transporters(m OCT1,m OCT2,m OCTN1 and m OCTN2)in the kidney were analyzed by Western blot,RT-PCR,immunohistochemical and immunofluorescent assay,respectively.RESULTS Wudang cherry significantly reduced serum uric acid levels and increased urine uric acid levels in hyperuricemic mice.And it effectively reversed potassium oxonate-induced alterations in renal m URAT1,mGLUT9,mOAT1,mOAT3 and mABCG2 m RNA and protein levels,resulting in the enhancement of renal urate excretion in mice.Moreover,Wudang Cherry increased renal m OCT1,m OCT2,m OCTN1 and m OCTN2 m RNA and protein levels,and improved renal impairment in this model.CONCLUSION Wudang cherry processes uricosuric and nephroprotective actions by regulating renal organic ion transporters in hyperuricemic mice.

Spatial Pattern and Process of Urbanization in Karst Area——A Case Study in Guiyang City

ObjectiveKarst area is one of ecological fragile regions in China, where the economic and social development is lagged heavily. In recent years, with the constant development of the west regions, economy and society are developed rapidly and urbanization speed is accelerated. This study aims to investigate the spatial pattern and process of urbanization in karst area of Guiyang City, which has important significance for land management and urban planning in karst area. MethodBased on RS and GIS technologies, TM satellite images in 1986, 1995 and 2000 and CBERS-01 satellite image in 2006 were adopted to obtain the data of urban land utilization in Guiyang City in the four years. The spatial pattern and process of urbanization in Guiyang City were investigated using urbanization proportion index （UPI）, urbanization intensity index （UII） and absolute expansion intensity （AEI）. ResultThe urbanization process in Guiyang City was significant, exhibiting polygon-urbanization, line-urbanization and point-urbanization. From the perspective of spatial pattern, the urbanization in Guiyang City displayed the cluster development; from the perspective of urbanization process, the urbanization speed in some secondary central areas was higher than that of central areas. ConclusionThe spatial pattern and process of urbanization in karst area were unique and were mainly influenced by terrain, transportation, economy, society and so on.

Geochemical characteristics of soil selenium and evaluation of Serich land resources in the central area of Guiyang City,China

Based on the results of multipurpose regional geochemical surveys of the Guizhou Province, geochemical characteristics of soil Se and Se-rich land resources in the central area of Guiyang City were studied and evaluated.Major conclusions are as follows:(1) the Se content in surface soil of the central area of Guiyang City was 0.17–2.89 mg kg^(-1), and the average was 0.78 mg kg^(-1), which were respectively 2.6 and 3.9 times of the national background value of soil and the world background value of soil.The Se content in deep soil was 0.11–1.48 mg kg^(-1), and the average was 0.44 mg kg^(-1), which were respectively 1.5 and2.2 times of the national background value of soil and the world background value of soil. The soil Se content decreased with the increase in the soil depth on the vertical profile, and the surface soil had a higher Se content.(2)Distribution of Se content was mainly affected by parent material, physicochemical properties of soil and other components, soil type, and land use type. Parent material played a key role, as the soil Se content was mainly originated from parent rock and increased with the background value of Se in parent rock, physicochemical properties of soil and other components had certain influences upon the Se content. Se was shown to have a significant linear positive correlation with S and organic carbon but no significant correlation with p H value. Se content varied with different types of soil as follows: skeleton soil > yellow soil > paddy soil > limestone soil > purple soil. Land use type also hadcertain influences upon the soil Se content as follows: dry land > construction land > garden plot > grassland =garden plot > forest land.(3) Taking 0.4 mg kg^(-1)B x(Se) <3.0 mg kg^(-1) as the standard for Se-rich soil, Se-rich soil of the study area covered an area of 2224 km^2 and 92.5% of the total area; the remaining is general soil. The study area had no Se-excess soil. Therefore, the central area of Guiyang City has a high proportion of Se-rich land, a large area of Serich land resources, and a moderate selenium enrichment strength, which have been rarely seen anywhere and provide advantageous resources for the development of Se-rich featured agriculture.

Wudang Mode of Ecological Civilization Construction in Western Counties

First, emphasis was given to the analysis on the practices of ecological civilization construction in Wudang District, and then the Wudang mode of ecological civilization construction was discussed and summarized from the aspects of implementation process, implementation path, and implementation dynamics, obtaining the following enlightenments： accelerating the development of ecological economy is the primary task for the ec- ological civilization construction in western counties; optimizing the spatial development pattern is an important means to promote the ecological civi- lization construction scientifically; capturing the acting point is the key factor to realize the win-win of economic development and ecological protection; the overall development of social undertakings to achieve the happiness of people in rural and urban area is the goal of ecological civilization.

Wudang Ancient Buildings

THE palaces and temples of the ancient building complex in the Wudang Mountains exemplify the architectural and artistic achievements of China＇s secular and religious buildings of the Yuan （1279-1368）. Ming （1368- 1644） and Qing （1644-1911） dynasties. Towering mountains and deep valleys are partnered with nearby woods, rocks and streams, presenting a harmonious picturesque fusion of man-made architecture and nature.

Ultrasonographic measurement of median nerve cross-sectional area reference values in a healthy Han population from Guiyang,China

High-resolution ultrasonography was used to analyze the nerve cross-sectional area （CSA） of the median nerve at 7 sites： the wrist crease, pisiform bone, hamate bone, 6 cm proximal to the tip of the wrist crease, proximal forearm （where the nerve enters the pronator teres muscle）, 4 cm proximal to the tip of the medial epicondyle, and mid-humerus （mid-point between elbow crease and axilla） in 200 healthy volunteers from Guiyang, China. Results showed similar CSA values between the left and right sides, but the CSA 6 cm proximal to the tip of the wrist crease, proximal forearm, 4 cm proximal to tip of the medial epicondyle, and mid-humerus in males was greater than that of females. Moreover, CSA values at the wrist crease, pisiform bone, and hamate bone were greater in the middle-aged and old groups when compared to the young group, and correlated with body mass and height. Thus, reference values of median nerve CSA of the upper limbs can facilitate the analysis of abnormal nerve conditions.

Changes of hydrochemical composition and heavy metals concentration in shallow groundwater from karst hilly areas in Guiyang region,China

The quality changes of shallow groundwater from karst hilly areas in Guiyang region of China impacted by the urbanization were investigated. The results show that the major ions in shallow groundwater from the karst hilly areas are mainly composed of HCO3^-, SO4^2-, Ca^2＋ and Mg^2＋, and the concentrations scopes of NO3^-, Cl^-, K^＋ and Na^＋ of the groundwater in agricultural, residential and industrial areas are 4.5-9.6, 2.8-7.1, 3.9-6.3 and 2.5-4.9 times higher than those in the forest areas, respectively. The concentrations of As, Pb and Cd of shallow groundwater in the industrial areas are also significantly enhanced, followed by those in the residential areas and the agricultural areas. The concentrations of NO3^-, SO4^2- , As, Pb and Cd of the groundwater in the industrial areas and those of NO3^- , SO4^2-, As and Cd of shallow groundwater in the residential areas reach grade Ⅲ of the Groundwater Quality Standard of China （GB/T 14848-93）, while the concentration of NO3^- in the groundwater from the industrial areas exceeds grade V. With the process of urbanization, NO3^- is the key factor to influence the groundwater quality in karst hilly areas, followed by SO4^2-, As, Pb and Cd.

The distribution characteristics of heavy metals in Guiyang urban soils

Guiyang is located in southwestern China,which is a famous tourism city,and is awarded the title of"the Summer Capital of China".A study on heavy metals in Guiyang urban soils was conducted.The results showed that the concentrations of Cd,Cu,Ni,Pb and Zn in Guiyang urban soils are significantly higher than their background values and Cu,Zn and Pb in Guiyang soils are accumulated seriously.The distribution characteristics of heavy metals in 3 districts are described as follows:the level of heavy metals in soils follows the order of Nanming District> Yunyan District>Xiaohe District.The spatial distribution of metals in urban soils of Guiyang shows that the level of metals in the central and eastern parts of Guiyang is relatively high,and its characteristics are attributed to the influence of commercial activities,heavy traffic and industrial emission.Compared with some other cities in China,Guiyang is characterized by relatively high accumulation level of Cd,Cu,and Zn in its soils.

The geochemical characteristics of aqueous rare-earth elements in shallow karst groundwater in Guiyang City, China

Fifty-seven shallow groundwater samples were collected from Guiyang karst basin, China, to analyze the aqueous rare-earth elements in low-water seasons and it is shown that the total amount of rare-earth elements (ΣREE) in karst groundwater is exceedingly low compared with that in carbonate rocks or weathering crusts of carbonate rocks, and ranges from 0.01 to 0.43, from 0.03 to 0.27, from 0.03 to 0.19 and from 0.05 to 1.38 μg·L-1 for dolomite, dolomitic & limestone, limestone and clastic rock aquifer, respectively. Both distributions and contents of rare-earth elements (REE) in karst groundwater reflect the lithology of host rocks or weathering crusts of carbonate rocks through which groundwater flows. The chondrite-normalized patterns show a non-flat profile with higher enrichment of slightly light rare-earth elements (LREE) than heavy rare-earth elements (HREE), prominent fractionation between LREE and HREE, negative Ce anomalies and negative or positive Eu anomalies. There is more obvious fractionation between LREE and HREE in groundwater than that in carbonate rocks and their weathering crusts due to high contents of HCO3? and PH in groundwater. In shallow karst groundwater, REE(CO3)n2n-3 (n=1 and 2) is the main inorganic species of REE. But for a clastic rock aquifer, both REESO4+ and REECO3+ are the main inorganic species of REE. Species of REE in groundwater is closely associated with the hydrochemical type of groundwater which is predominated by the lithology of host rocks, groundwater-rock interaction and weathering-pedogenesis of carbonate rocks.

Distribution and sequential extraction of some heavy metals in urban soils of Guiyang City,China

Sixty-two soil samples collected from different functional zones of Guiyang were analyzed for total con-centrations and sequential extraction of Cr,Cu,Pb,Zn and Cd by ICP spectrometry. The average total concentrations of Cr,Cu,Pb,Zn and Cd in the soils of Guiyang were 92.9,51.6,44.1,139.3 and 0.28 mg/kg,respectively. The soils have been polluted by Cr,Cu,Pb,Zn and Cd to some extent in comparison with the background values of Guiyang. Significant differences were recognized in the concentrations of Cr,Cu,Pb,Zn and Cd in different functional zones. As for the sequential extraction,Cr,Cu and Zn were present mainly in the residual fraction,and Pb was present mainly in the oxidizable fraction. The reducible fraction of Cd accounts for 47.5%,and the residual fraction is lowest. The mobility and bioavailability of heavy metals follow the order of Cd>Pb>Cu>Cr>Zn.

Geogenic distribution and baseline concentrations of Cu and Zn in surficial soil of Guiyang, China

This paper describes the environmentally geochemical characteristics of heavy metal pollutants such as Cu and Zn in the surficial soil of Guiyang, the capital city of Guizhou Province. The baseline concentrations of heavy metals in soil and the geochemical norms have been established to distinguish natural or artificial influence on the environment. Using the statistically analytical methods, we have established the baseline of Cu in Guiyang ({18.8} mg/kg) and the soil (over {68.4} mg/kg) may suffer Cu contamination. The data of Zn are {46.3} mg/kg and {112.0} mg/kg. Geoaccumulation index analysis indicated that the surficial soil that has suffered non-pollution to mid-pollution of Cu accounts for 38%, 38%, and 22% for mid-pollution, and only 2% between mid|pollution to severe pollution. Surficial soil that has suffered non-pollution to mid-pollution of Zn accounts for 41%, 50%, and 7% for mid-pollution, and only 2% between mid-pollution to severe pollution. The maximal degree of Cu contamination is 5.09 in the surficial soil of Guiyang. Surficial soil that has not suffered Cu pollution accounts for {53.2%}. The total pollution degree of Cu is slightly over zero. So the surficial soil of Guiyang has suffered slight Cu pollution. The maximum value of Zn contamination degree is {2.85} in the surficial soil of Guiyang. {77.8%} of the surficial soil has not yet suffered any Zn pollution. The total pollution degree of Zn is less than zero. So the surficial soil in Guiyang is free from pollution of Zn.

Study on the Sr, Nd, Pb and O Isotopes of Basic Dyke Swarms in the Wudang Block and Basic Volcanics of the Yaolinghe Group

The Sr, Nd and Pb isotopic characteristics of the Wudang basic dyke swarms and basic volcanics of the Yaolinghe Group show that they were derived from the same multi\|component mixing source in the mantle. The Wudang basic dyke swarms have (\{\}\+\{87\}Sr/\{\}\+\{86\}Sr)\-i=\{0.6905\}～\{0.7061\}, ε\-\{Nd\}(t)=\{-1.9\}～\{5.0\}, Δ\{\}\+\{208\}Pb/\+\{204\}Pb=\{35.49\}～\{190.26\}, Δ\{\}\+\{207\}Pb/\{\}\+\{204\}Pb=4～85, low Th/Ta and a wide range of La/Yb ratios; and the basic volcanics of the Yaolinghe Group have (\{\}\+\{87\}Sr/\{\}\+\{86\}Sr)\-i=\{0.6487\}～\{0.7075\}, ε\-\{Nd\}(t)=\{0.11\}～\{3.94\}, Δ\{\}\+\{208\}Pb/\{\}\+\{204\}Pb=\{-81.58\}～\{219.95\}, Δ\{\}\+\{207\}Pb/\{\}\+\{204\}Pb=\{4.44\}～\{16.68\} and higher Th/Ta and La/Yb ratios, indicating that their source is a mixture of DM and EMII, and the basic volcanics of the Yaolinghe Group were contaminated by crust materials en rout to the surface. Based on the geochemical features of continental tholeiitic basalts and being products of different facies derived from the same source, it can be concluded that an important rifting event in the South Qinling basement block occurred during Neoproterozoic, followed by a setting of oceanic basin in the Early Paleozoic.

Accumulation and risk assessment of heavy metals in dust in main living areas of Guiyang City, Southwest China

Guiyang is a famous tourist city located in southwestern China. In this study, dust from eleven residential areas, seven city squares, and nine schools was collected to measure the heavy metal levels and evaluate its risk. At each sampling site, 4–5 sub-samples were taken as a bulk sample. All samples were air-dried, ground, passed through a 0.105 mm nylon sieve, digested with HNO3-HClO4 to determine the concentrations of Cd, Cu, Ni, Pb and Zn by ICP-MS, and digested with 1:1 aqua regia to determine As by AFS. The results show that the concentrations of As, Cd, Cu, Ni, Pb and Zn in dust of Guiyang City follow normal distribution with means of 16.1, 1.54, 138, 47.7, 129 and 479 mg/kg, respectively. Levels of As, Cd, Cu, Ni, Pb and Zn exceed the background level of soil in Guizhou Province by 33%, 96%, 100%, 78%, 96%, and 100%, respectively. Cd, Cu, Pb and Zn are heavily accumulated in dust of living areas with accumulation factors of 4.10, 5.12, 4.12 and 5.51, respectively. City square possesses the highest geometric means of As, Cd, Cu, Pb, and Zn. The risks of heavy metal exposure to teenagers are not obvious and in an order of As>Pb>Cu>Ni>Zn(Cd).

Economic Cost Analysis of the Urban Sustainable Development——A Case of Guiyang City,China

Based on the constitution identification of the urbanization economic costs,econometric model of urbanization economic costs is constructed from four aspects of the investment cost in employment opportunities,the investment cost in urban land use,the investment cost in urban infrastructure,and the investment cost in urban management.Taking Guiyang City as an example,economic cost for each additional urban population in Guiyang City is measured under the current level of social and economic development.Capital requirement for the urban development of Guiyang City in the next 10 years is forecasted.And it is pointed out that there will be significant financing gap in the sustainable development and urbanization process of Guiyang City in future,which provides the basis for the development speed and the reasonable goals of urban sustainable development in future.

Geochemistry of Basic Dykes in Wudang Block and Its Tectonic Significance

Geochemical characteristics of the Neoproterozoic basic dykes widely exposed in the Wudang block of South Qinling show that the dykes possess the characteristics of continental tholeiitic basalts and were formed in a setting of continental rifting. Therefore, there was an ancient continental block in the areas of southern Qinling and Yangtze block during the Jinning period, and an important rifting event of this ancient block occurred in the Early Neoproterozoic.

Call For Papers: The 2019 China Eco-civilization Construction Guiyang Forum

The report of the Nineteenth National Congress of the Communist Party of China clearly points out that we should speed up the reform of the ecological civilization system and build a beautiful China.Guizhou Province is one of the first demonstration zones of ecological civilization established by the State Council and themacro-ecological strategy is one of the three strategic actions of Guizhou Province.