Objective:To explore the presence of inflammatory memory in the lung tissue of mice with cough variant asthma(CVA)and the mechanism of Wuhu decoction in preventing and treating CVA.Methods:Among the 90 SPF-grade femal...Objective:To explore the presence of inflammatory memory in the lung tissue of mice with cough variant asthma(CVA)and the mechanism of Wuhu decoction in preventing and treating CVA.Methods:Among the 90 SPF-grade female BALB/c mice,20 were randomly selected as the blank group,while the remaining 70 were sensitized by intraperitoneal injection of ovalbumin(OVA)combined with aluminum hydroxide and then exposed to OVA by nebulization to establish the CVA mouse model.From the conctrol group and the model group,10 mice were randomly selected to detect airway responsiveness using an animal ventilator.Successfully modeled mice were then randomly divided into 7 groups:a CVA model group,a CVA re-challenge group,low-,medium-,and high-dose Wuhu decoction groups,and a positive control(dexamethasone)group,with 10 mice in each group.After 30 days of rest,except for the conctrol group and the model group,mice in the other groups underwent a second OVA nebulization challenge and received corresponding drug interventions once a day for 10 consecutive days before further analysis.Pathological changes in lung tissue were observed using periodic acid-schiff(PAS)and Masson staining methods.Serum levels of tumor necrosis factor-α(TNF-α),interleukin-4(IL-4),interferon-γ(IFN-γ),and interleukin-10(IL-10)were detected using enzyme-linked immunosorbent assay(ELISA).The expression of T-bet mRNA and GATA-3 mRNA in lung tissue was determined by real-time quantitative polymerase chain reaction(Real-time PCR).Results:The results of airway responsiveness indicated successful establishment of the model.Compared with the control group,the model group exhibited severalkey pathological changes including inflammatory cellinfiltration around the lung tissue,epithelialmetaplasia,airway collagen deposition,increased airway mucus accumulation index and airway collagen deposition index(P<0.05),elevated serum TNF-αand IL-4 expression(P<0.05),decreased IFN-γand IL-10 levels(P<0.05),decreased T-bet mRNA levels(P<0.05),increased GATA-3 mRNA levels(P<0.05),and a reduced T-bet mRNA/GATA-3 mRNA ratio(P<0.05)in lung tissue.When compared to the CVA re-challenge group,the Wuhu decoction groups and the dexamethasone group effectively alleviated pathological changes in lung tissue,reduced the airway mucus accumulation index and airway collagen deposition index(P<0.05),decreased serum TNF-αand IL-4 expression(P<0.05),increased serum IFN-γand IL-10 levels(P<0.05),upregulated T-bet mRNA levels(P<0.05),downregulated GATA-3 mRNA levels(P<0.05),and increased the T-bet mRNA/GATA-3 mRNA ratio(P<0.05)in lung tissue.Conclusion:The lung tissue of mice with cough variant asthma exhibits inflammatory memory,and Wuhu decoction may intervene in this inflammatory memory by restoring the balance between T-bet and GATA-3,thereby correcting the imbalance in Th1/Th2 immunity and exerting a preventive and therapeutic effect on cough variant asthma.展开更多
The increasing availability of data in the urban context(e.g.,mobile phone,smart card and social media data)allows us to study urban dynamics at much finer temporal resolutions(e.g.,diurnal urban dynamics).Mobile phon...The increasing availability of data in the urban context(e.g.,mobile phone,smart card and social media data)allows us to study urban dynamics at much finer temporal resolutions(e.g.,diurnal urban dynamics).Mobile phone data,for instance,are found to be a useful data source for extracting diurnal human mobility patterns and for understanding urban dynamics.While previous studies often use call detail record(CDR)data,this study deploys aggregated network-driven mobile phone data that may reveal human mobility patterns more comprehensively and can mitigate some of the privacy concerns raised by mobile phone data usage.We first propose an analytical framework for characterizing and classifying urban areas based on their temporal activity patterns extracted from mobile phone data.Specifically,urban areas’diurnal spatiotemporal signatures of human mobility patterns are obtained through longitudinal mobile phone data.Urban areas are then classified based on the obtained signatures.The classification provides insights into city planning and development.Using the proposed framework,a case study was implemented in the city of Wuhu,China to understand its urban dynamics.The empirical study suggests that human activities in the city of Wuhu are highly concentrated at the Traffic Analysis Zone(TAZ)level.This large portion of local activities suggests that development and planning strategies that are different from those used by metropolitan Chinese cities should be applied in the city of Wuhu.This article concludes with discussions on several common challenges associated with using network-driven mobile phone data,which should be addressed in future studies.展开更多
Based on the domestic and foreign related research methods, the life meteorological index forecasting system of Wuhu City was compiled using database and network as well as computer language. The system realized the a...Based on the domestic and foreign related research methods, the life meteorological index forecasting system of Wuhu City was compiled using database and network as well as computer language. The system realized the automation process for the generation of life index forecasting products from local situation of Wuhu City and forecasting data, which could get the latest service products dispensing with manual intervention. The development of the system not only made the operation process of the life meteorological index of Wuhu City more time-saving and efficient, but also made the results more scientific and rigorous.展开更多
Wuhu is one of the three major tourist centers in Anhui Province, and the first city to be built and operated in China＇ s fourth generation theme park. Special series of theme parks have been built from the operation...Wuhu is one of the three major tourist centers in Anhui Province, and the first city to be built and operated in China＇ s fourth generation theme park. Special series of theme parks have been built from the operation, make Wuhu city transit tourism city has become a tourist destination city. In recent years, the pattern of Wuhu＇ s over dependence on theme parks has become increasingly prominent. Under this background, characteristics and the city tourism interaction status clear Wuhu theme park, theme park and interactive strategy to explore the development of city tourism, such as the concept of innovation, establish one king city development strategy, group development, build Wuhu tourism cluster, increase independent efforts to stimulate innovation cluster. The analysis method and combining with the on-the-spot investigation, facing Wuhu Fantawild theme park development strengths, weaknesses, opportunities and challenges are analyzed, and on this basis to improve the competitiveness of Wuhu Fantawild theme park marketing strategy.展开更多
Located in the southeast of AnhuiProvince, beside the Yangtze River, and atthe west verge of the Yangtze Delta, Wuhu isan open riverside city approved by the state,and a port opening to foreign ships,performing a link...Located in the southeast of AnhuiProvince, beside the Yangtze River, and atthe west verge of the Yangtze Delta, Wuhu isan open riverside city approved by the state,and a port opening to foreign ships,performing a link function in the generalstrategy of the Yangtze River, with Shanghaias the head and three gorges as the pillar. The Wuhu Economic and TechnicalDevelopment Zone, approved by the StateCouncil is the key point to developingWanjiang and responding Pudong. With aplanned area of 10 sq km, the zone is close展开更多
文摘Objective:To explore the presence of inflammatory memory in the lung tissue of mice with cough variant asthma(CVA)and the mechanism of Wuhu decoction in preventing and treating CVA.Methods:Among the 90 SPF-grade female BALB/c mice,20 were randomly selected as the blank group,while the remaining 70 were sensitized by intraperitoneal injection of ovalbumin(OVA)combined with aluminum hydroxide and then exposed to OVA by nebulization to establish the CVA mouse model.From the conctrol group and the model group,10 mice were randomly selected to detect airway responsiveness using an animal ventilator.Successfully modeled mice were then randomly divided into 7 groups:a CVA model group,a CVA re-challenge group,low-,medium-,and high-dose Wuhu decoction groups,and a positive control(dexamethasone)group,with 10 mice in each group.After 30 days of rest,except for the conctrol group and the model group,mice in the other groups underwent a second OVA nebulization challenge and received corresponding drug interventions once a day for 10 consecutive days before further analysis.Pathological changes in lung tissue were observed using periodic acid-schiff(PAS)and Masson staining methods.Serum levels of tumor necrosis factor-α(TNF-α),interleukin-4(IL-4),interferon-γ(IFN-γ),and interleukin-10(IL-10)were detected using enzyme-linked immunosorbent assay(ELISA).The expression of T-bet mRNA and GATA-3 mRNA in lung tissue was determined by real-time quantitative polymerase chain reaction(Real-time PCR).Results:The results of airway responsiveness indicated successful establishment of the model.Compared with the control group,the model group exhibited severalkey pathological changes including inflammatory cellinfiltration around the lung tissue,epithelialmetaplasia,airway collagen deposition,increased airway mucus accumulation index and airway collagen deposition index(P<0.05),elevated serum TNF-αand IL-4 expression(P<0.05),decreased IFN-γand IL-10 levels(P<0.05),decreased T-bet mRNA levels(P<0.05),increased GATA-3 mRNA levels(P<0.05),and a reduced T-bet mRNA/GATA-3 mRNA ratio(P<0.05)in lung tissue.When compared to the CVA re-challenge group,the Wuhu decoction groups and the dexamethasone group effectively alleviated pathological changes in lung tissue,reduced the airway mucus accumulation index and airway collagen deposition index(P<0.05),decreased serum TNF-αand IL-4 expression(P<0.05),increased serum IFN-γand IL-10 levels(P<0.05),upregulated T-bet mRNA levels(P<0.05),downregulated GATA-3 mRNA levels(P<0.05),and increased the T-bet mRNA/GATA-3 mRNA ratio(P<0.05)in lung tissue.Conclusion:The lung tissue of mice with cough variant asthma exhibits inflammatory memory,and Wuhu decoction may intervene in this inflammatory memory by restoring the balance between T-bet and GATA-3,thereby correcting the imbalance in Th1/Th2 immunity and exerting a preventive and therapeutic effect on cough variant asthma.
基金Under the auspices of the National Natural Science Foundation of China(No.41571146)China Postdoctoral Science Foundation(No.2019M651784)。
文摘The increasing availability of data in the urban context(e.g.,mobile phone,smart card and social media data)allows us to study urban dynamics at much finer temporal resolutions(e.g.,diurnal urban dynamics).Mobile phone data,for instance,are found to be a useful data source for extracting diurnal human mobility patterns and for understanding urban dynamics.While previous studies often use call detail record(CDR)data,this study deploys aggregated network-driven mobile phone data that may reveal human mobility patterns more comprehensively and can mitigate some of the privacy concerns raised by mobile phone data usage.We first propose an analytical framework for characterizing and classifying urban areas based on their temporal activity patterns extracted from mobile phone data.Specifically,urban areas’diurnal spatiotemporal signatures of human mobility patterns are obtained through longitudinal mobile phone data.Urban areas are then classified based on the obtained signatures.The classification provides insights into city planning and development.Using the proposed framework,a case study was implemented in the city of Wuhu,China to understand its urban dynamics.The empirical study suggests that human activities in the city of Wuhu are highly concentrated at the Traffic Analysis Zone(TAZ)level.This large portion of local activities suggests that development and planning strategies that are different from those used by metropolitan Chinese cities should be applied in the city of Wuhu.This article concludes with discussions on several common challenges associated with using network-driven mobile phone data,which should be addressed in future studies.
文摘Based on the domestic and foreign related research methods, the life meteorological index forecasting system of Wuhu City was compiled using database and network as well as computer language. The system realized the automation process for the generation of life index forecasting products from local situation of Wuhu City and forecasting data, which could get the latest service products dispensing with manual intervention. The development of the system not only made the operation process of the life meteorological index of Wuhu City more time-saving and efficient, but also made the results more scientific and rigorous.
文摘Wuhu is one of the three major tourist centers in Anhui Province, and the first city to be built and operated in China＇ s fourth generation theme park. Special series of theme parks have been built from the operation, make Wuhu city transit tourism city has become a tourist destination city. In recent years, the pattern of Wuhu＇ s over dependence on theme parks has become increasingly prominent. Under this background, characteristics and the city tourism interaction status clear Wuhu theme park, theme park and interactive strategy to explore the development of city tourism, such as the concept of innovation, establish one king city development strategy, group development, build Wuhu tourism cluster, increase independent efforts to stimulate innovation cluster. The analysis method and combining with the on-the-spot investigation, facing Wuhu Fantawild theme park development strengths, weaknesses, opportunities and challenges are analyzed, and on this basis to improve the competitiveness of Wuhu Fantawild theme park marketing strategy.
文摘Located in the southeast of AnhuiProvince, beside the Yangtze River, and atthe west verge of the Yangtze Delta, Wuhu isan open riverside city approved by the state,and a port opening to foreign ships,performing a link function in the generalstrategy of the Yangtze River, with Shanghaias the head and three gorges as the pillar. The Wuhu Economic and TechnicalDevelopment Zone, approved by the StateCouncil is the key point to developingWanjiang and responding Pudong. With aplanned area of 10 sq km, the zone is close
