Research Hotspot and Trend Analysis of Wuling Powder Based on CiteSpace

[Objectives]This study was conducted to systematically present the research and development status of Wuling Powder over the years, and comprehensively analyze the hot spots and trends of Wuling Powder in the treatment of diseases, so as to provide reference for scholars in this field in the future. [Methods] China National Knowledge Network(CNKI) database was used as the literature retrieval source to search the literature related to Wuling Powder in the treatment of nephropathy, and then the data visualization analysis was carried out by using the software citespace.5.7.R2. [Results] Through accurate retrieval, a total of 204 effective literatures were included. It was found that domestic research on the treatment of nephropathy by Wuling Powder was on the rise. From the analysis of knowledge graph, it was concluded that after long-term research and development, a relatively concentrated core research group had been formed in China, which mainly focused on "Wuling Powder", "chronic kidney disease" and "diabetic nephropathy". [Conclusions] The research on the treatment of nephropathy by Wuling Powder shows a good development trend, and the research content is constantly enriched, and this research is still the current and future research hotspot.

Effects of Wuling Powder Mediating Notch Pathway on Mice with Nephrotic Syndrome

[Objectives]This study was conducted to investigate the renal protective effects of Wuling Powder on mice with nephrotic syndrome(NS)based on Notch pathway.[Methods]Sixty KM mice were randomly divided into normal group,model group,prednisone acetate positive group,high-dose Wuling Powder group,medium-dose Wuling Power group and low-dose Wuling Power group,with 10 mice in each group.Three days after prophylactic administration,a comprehensive nephropathy model was prepared by injecting 1 mg/ml doxorubicin hydrochloride solution(7.5 mg/kg)into the tail vein.After successful modeling,prednisone acetate and Wuling SAN were given high,medium and low doses for intervention for 28 d,respectively.After that,urinary protein and creatinine contents of mice in each group were detected,and pathological damage of renal tissue was observed by HE and Masson staining.The mRNA levels of Notch1,Jagged1 and Hes1 in mouse kidney tissues were detected by RT-PCR,and the expression levels of Notch1,Jagged1 and Hes1 proteins were detected by Western blot.[Results]Wuling Powder could effectively reduce the contents of urine protein(P<0.01)and Scr(P<0.01)in NS mice,and alleviate the pathological injury of kidney.Compared with the model group,the prednisone acetate group and various Wuling Powder groups could down-regulate the expressions of Notch1,Jagged1 and Hes1 mRNA in the kidney tissue of mice(P<0.01),and the expression of Notch1 protein in the renal tissue of mice decreased(P<0.01).The contents of Hes1 in the prednisone acetate group and the high-and medium-dose Wuling Powder groups significantly decreased(P<0.05).[Conclusions]Wuling Powder could protect the kidneys in mice with NS through Notch pathway.

Constructing a Core Collection for Maize (Zea mays L.) Landrace from Wuling Mountain Region in China

Based on the genetic clustering from 42 microsatellite （SSR） markers with a combination of their geographic origin and germplasm characteristics, 124 maize landraces from Wuling Mountain region in China were used for constructing a core collection. Four evaluating parameters for maize landrace core collection, including mean difference percentage （MD）, variance difference percentage （VD）, coincidence rate of range （CR）, and variable rate of coefficient of variation （VR）, were assessed With 20 quantitative traits. It was shown that genetic relationships among landraces in Wuling Mountain region had the tendency to associate with their geographic origins. The 124 landraces were clustered into 18 subgroups when the coefficient of genetic similarity （GS） is 0.28. Eighteen landraces, each of which was from one subgroup, were applied to construct the core collection with a sampling percentage of 15%. Comparison of the initial and core collection indicated that there existed no significant differences in most quantitative traits. An average of 6.3 and 6.5 alleles were detected in the initial and core collection, respectively. Mean polymorphism information content in the core collection （0.75） was higher than that in the initial one （0.72）. MD was lesser than 20% and CR was more than 80%. The results showed that the sampling strategy would be feasible for constructing the core collection that well represents the genetic diversity of the initial one.

Zircon U-Pb Age,Geochemical,and Sr-Nd-Pb-Hf Isotopic Constraints on the Time Frame and Origin of Early Cretaceous Mafic Dykes in the Wuling Mountain Gravity Lineament,South China

In view of the importance of mafic dyke swarms and their contribution to current scientific problems relating to South China,herein,we present the findings of studies on twenty–five representative mafic dykes cropping out in Hunan Province and Guangxi Zhuang Autonomous Region,within the southern Wuling Mountain gravity lineament,China.These results include new zircon LA-ICP-MS U-Pb age,whole rock geochemical,Sr-Nd-Pb isotopic,and zircon Hf isotopic data for these dykes.The dykes formed between 131.5±1.2 and 121.6±1.1 Ma,and have typical doleritic textures.They fall into the alkaline and shoshonitic series,are enriched in light rare earth elements(LREE),some large ion lithophile elements(LILE;e.g.,Rb,Ba,and Sr),Th,U,and Pb,and are depleted in Nb,Ta,Hf,and Ti.Moreover,the dolerites have high initial 87 Sr/86 Sr ratios(0.7055–0.7057),negativeεNd(t)and zirconεHf(t)values(-14.8 to-11.9,-30.4 to-14.9),and relatively constant initial Pb isotopic ratios(that are EM1-like,16.77–16.94,15.43–15.47,and 36.84–36.92 for 206 Pb/204 Pb,207 Pb/204 Pb,and 208 Pb/204 Pb,respectively).These results indicate that the dykes were likely derived from magma generated through low-degree partial melting(1.0%–10%)of an EM1-like garnet–lherzolite mantle source.The parental magmas fractionated olivine,clinopyroxene,plagioclase,and Ti-bearing phases with negligible crustal contamination,during ascent and dyke emplacement.Several possible models have been proposed to explain the origin of Mesozoic magmatism along the Wuling Mountain gravity lineament.Herein we propose a reasonable model for the origin of these mafic dykes,involving the collision between the paleo-Pacific Plate and South China,which led to subsequent lithospheric extension and asthenosphere upwelling,resulting in partial melting the underlying mantle lithosphere in the Early Cretaceous,to form the parental magmas to the WMGL mafic dykes,as studied.

Efficacy and Safety of Wuling Powder and Western Medicine in Treatment of Hyperuricemia:A Meta-analysis of Clinical Trials

[Objectives]To compare the clinical efficacy and safety of Wuling powder and conventional Western medicine for lowering uric acid in the treatment of hyperuricemia.[Methods]Databases such as CNKI,Wanfang Data,VIP Database,PubMed,Cochrane library and Embase,were retrieved,and comparative experiments of efficacy(serum uric acid level)and safety(incidence of adverse reactions)between Wuling powder(used singly or combined with other prescriptions)and conventional Western medicine in the treatment of hyperuricemia were included,and effect size was pooled with Revman 5.3.[Results]A total of 11 articles,involving 817 patients,were included,and their research quality was evaluated to be low.In terms of overall effect,Wuling powder(used singly or combined with other prescriptions)was better than the control drug in reducing serum uric acid level,but the difference was insignificant[SMD=-0.37,95%CI(-0.90,0.16)].The heterogeneity between studies was high.The incidence of adverse reactions in the experimental group was significantly lower than that in the control group.Subgroup analysis shows that Wuling powder combined with other prescriptions was better than the control drug in reducing serum uric acid level[SMD=-0.37,95%CI(-0.90,0.16),P=0.17],and the heterogeneity was reduced.[Conclusions]Wuling powder combined with other prescriptions is better than conventional Western medicine in the treatment of hyperuricemia,and has better safety.Wuling powder alone is not as effective as conventional Western medicine.It is suggested that in clinical work,doctors may need to modify the prescription of Wuling powder.The low quality of the literature supporting this conclusion suggests that this evidence should be interpreted with caution.

Study on the action mechanism of Wuling Powder on treating osteoporosis based on network pharmacology

Osteoporosis is a health problem to cause global concern.A lot of methods have been used to prevent and treat osteoporosis,but there is still a lack of effective treatment for osteoporosis owing to limited understanding of its mechanism.Therefore,the aim of this present study is to explore the underlying mechanism of Wuling Powder,a traditional Chinese medicine on treating osteoporosis.In this study,we firstly screened and identified the common targets between Wuling Powder and osteoporosis through the related databases,and then explored the relationships among these targets,Wuling Powder and osteoporosis by using Gene Ontology(GO),Kyoto Encyclopedia of Genes and Genomes(KEGG)and network analyses.Subsequently,the molecular docking was performed by using systemsDock to evaluate the potential binding relationships between the active components of Wuling Powder and their related targets.The results showed that in total of 14 common targets including CREBBP,ADAM17,GOT1,GAPDH,USP8,ERBB2,EEF1 A1,MTOR,RAC1,ETS1,DDX58,GCK,EGF and S100 A8 were screened.EGF,ERBB2,MTOR and HIF-1 were the potential therapeutic targets for osteoporosis,and they were also the related targets for predicting active components in Wuling Powder.Taken together,we concluded that Wuling Powder might be used to treat osteoporosis through above these targets.

Simultaneous Determination of Eight Active Components in Liuwei Wuling Tablet Using HPLC

Objective To develop an effective method for simultaneous determination of eight major components in Liuwei Wuling Tablet（LWT）. Methods Reversed phase HPLC method was used with variously improved conditions. Results Salidroside, forsythoside A, specnuezhenide, phillyrin, schisandrol A, schizandrol A, schizandrin A, and schizandrin B in LWT were successfully separated on a Kromasil 100-5-C_（18） reverse phase column（250 mm × 4.6 mm, 5 μm） using acetonitrile -0.1% phosphoric acid（gradient elution） as mobile phase. The detection wavelength was 275 nm with flow rate of 1.0 mL/min, and the column temperature was maintained at 35 ℃. The recovery rate of the method was within the range of 95.13%-104.56% and the precision（RSD） was less than 3% for all eight analytes. All the compounds showed good linearities（r^2〉0.9980） in a relatively wide concentration range. Conclusion Simultaneous quantification of the multiple components by HPLC would be a better strategy for the quality evaluation of LWT.

Geochronology and Tectonic Evolution of the West Section of the Jiangnan Orogenic Belt

As an important part of South China Old Land, the Jiangnan Orogenic Belt plays a significant role in explaining the assembly and the evolution of the Upper Yangtze Block and Cathaysia, as well as the structure and growth mechanism of continental lithosphere in South China.The Lengjiaxi and the Banxi groups are the base strata of the west section of the Jiangnan Orogenic Belt.Thus, the research of geochronology and tectonic evolution of the Lengjiaxi and the Banxi groups is significant.The maximum sedimentary age of the Lengjiaxi Group is ca.862 Ma, and the minimum is ca.822 Ma.The Zhangjiawan Formation, which is situated in the upper part of the Banxi Group is ca.802 Ma.The Lengjiaxi Group and equivalent strata should thus belong to the Neoproterozoic in age.The Jiangnan Orogenic Belt consisting of the Lengjiaxi and the Banxi groups as important constituents is not a Greenville Orogen Belt（1.3 Ga–1.0 Ga）.The Jiangnan Orogenic Belt is a recyclic orogenic belt, and the prototype basin is a foreland basin with materials derived from the southwest and the sediments belong to the active continental sedimentation.By combining large amounts of dating data of the Lengjiaxi and the Banxi groups as well as equivalent strata, the evolutionary model of the western section of the Jiangnan Orogenic Belt is established as follows： Before 862 Ma, the South China Ocean was subducted beneath the Upper Yangtze Block, while a continental island arc was formed on the side near the Upper Yangtze Block.The South China Ocean was not closed in this period.From 862 Ma to 822 Ma, the Upper Yangtze Block was collided with Cathaysia; and sediments began to be deposited in the foreland basin between the two blocks.The Lengjiaxi Group and equivalent strata were thus formed and the materials might be derived from the recyclic orogenic belt.From 822 Ma to 802 Ma, Cathaysia continued pushing to the Upper Yangtze Block, experienced the Jinning-Sibao Movement（Wuling Movement）; as result, the folded basement of the Jiangnan Orogenic Belt was formed.After 802 Ma, Cathaysia and the Upper Yangtze Block were separated from each other, the Nanhua rift basin was formed and began to receive the sediments of the Banxi Group and equivalent strata.These large amounts of dating data and research results also indicate that before the collision of the Upper Yangtze Block with Cathaysia, materials of the continental crust became less and less from the southwest to the east in the Jiangnan Orogeneic Belt; only island arc and neomagmatic arc were developed in the eastern section.Ocean-continent subduction or continent-continent subduction took place in the western and southern sections, while intra-oceanic subduction occurred in the eastern section.Comprehensive analyses on U-Pb ages and Hf model ages of zircons, the main provenance of the Lengjiaxi Group is Cathaysia.