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MXene纳米颗粒Ti_(3)C_(2)T_(X)与光热效应促进糖尿病小鼠创面愈合
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作者 李美运 刘森 +5 位作者 陈开元 施灵 宋美陈 曹家洪 武艳 于晶 《中国组织工程研究》 CAS 北大核心 2025年第28期6052-6060,共9页
背景:MXene纳米颗粒具备出色的亲水性、生物相容性和抗菌特性,被广泛应用于创面、肿瘤、神经修复和心血管等治疗领域,目前尚不清楚MXene纳米颗粒对糖尿病创面愈合的作用。目的:考察MXene纳米颗粒Ti_(3)C_(2)T_(x)的体外抗氧化、抗炎和... 背景:MXene纳米颗粒具备出色的亲水性、生物相容性和抗菌特性,被广泛应用于创面、肿瘤、神经修复和心血管等治疗领域,目前尚不清楚MXene纳米颗粒对糖尿病创面愈合的作用。目的:考察MXene纳米颗粒Ti_(3)C_(2)T_(x)的体外抗氧化、抗炎和光热抗菌活性,以及对糖尿病小鼠创面的修复效果。方法:①体外实验:采用MTT法检测不同质量浓度Ti_(3)C_(2)T_(x)对小鼠成纤维细胞(NIH-3T3)的毒性作用。将NIH-3T3细胞暴露在H_(2)O_(2)中,采用MTT法检测不同质量浓度Ti_(3)C_(2)T_(x)对NIH-3T3细胞的保护作用。将NIH-3T3细胞暴露在H_(2)O_(2)中,分析光照(或不光照)处理下Ti_(3)C_(2)T_(x)(20μg/mL)对NIH-3T3细胞活性氧生成的影响。将RAW264.7巨噬细胞分3组处理:对照组、脂多糖组与脂多糖+Ti_(3)C_(2)T_(x)组,采用实时定量PCR法检测细胞中特定基因(CD86、白细胞介素6、CD206、精氨酸酶1)的表达。将大肠杆菌(或金黄色葡萄球菌)分3组处理:对照组、Ti_(3)C_(2)T_(x)组、Ti_(3)C_(2)T_(x)光照组,利用平板菌落计数法计算细菌存活率。②体内实验:通过腹腔注射链脲佐菌素的方法构建ICR小鼠糖尿病模型,造模成功后在小鼠背部建立全层皮肤缺损创面,随机分3组干预:对照组(n=6)、Ti_(3)C_(2)T_(x)组(n=6)和Ti_(3)C_(2)T_(x)光照组(n=6),观察创面愈合情况,干预后第7天进行创面组织CD31、CD206免疫组化染色,干预后第7,14天进行创面组织苏木精-伊红与Masson染色。将Ti_(3)C_(2)T_(x)溶液注射至ICR小鼠皮下,在光照(或非光照)后,通过血生化检测分析Ti_(3)C_(2)T_(x)对小鼠的毒性作用。结果与结论:①体外实验:Ti_(3)C_(2)T_(x)质量浓度在5-160μg/mL范围内对NIH-3T3细胞无毒性作用,在质量浓度20μg/mL时可增加NIH-3T3细胞存活率。10-80μg/mL的Ti_(3)C_(2)T_(x)可显著提升H_(2)O_(2)干预下的NIH-3T3细胞存活率。Ti_(3)C_(2)T_(x)可显著抑制H_(2)O_(2)干预下NIH-3T3细胞活性氧的生成,光照处理可进一步提升Ti_(3)C_(2)T_(x)抑制活性氧生成的作用。Ti_(3)C_(2)T_(x)能够有效抑制由脂多糖诱导的巨噬细胞炎症,促进细胞向具有抗炎特性的M2型巨噬细胞转化。Ti_(3)C_(2)T_(x)与Ti_(3)C_(2)T_(x)光照均可显著抑制大肠杆菌和金黄色葡萄球菌的生长,并且Ti_(3)C_(2)T_(x)光照的抑制效果更显著。②体内实验:创面大体与组织学分析显示,Ti_(3)C_(2)T_(x)与Ti_(3)C_(2)T_(x)光照均可促进糖尿病小鼠创面的愈合,并且Ti_(3)C_(2)T_(x)光照的促进作用更显著。免疫组化染色结果显示,Ti_(3)C_(2)T_(x)与Ti_(3)C_(2)T_(x)光照均可抑制糖尿病创面的炎症反应并促进血管的生成,并且Ti_(3)C_(2)T_(x)光照的作用更显著。血生化检测结果显示,Ti_(3)C_(2)T_(x)与光照对小鼠无明显毒性作用。③结果表明,Ti_(3)C_(2)T_(x)借助独特的光热效应能够高效地展现抗氧化、抗炎和抗菌特性,促进糖尿病小鼠创面的愈合。 展开更多
关键词 Ti_(3)C_(2)T_(x) Mxene纳米颗粒 糖尿病创面 抗氧化 抗炎 抗菌 工程化创面材料
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Cellular interplay to 3D in vitro microphysiological disease model:cell patterning microbiota-gut-brain axis 被引量:1
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作者 Kamare Alam Lakshmi Nair +6 位作者 Souvik Mukherjee Kulwinder Kaur Manjari Singh Santanu Kaity Velayutham Ravichandiran Sugato Banerjee Subhadeep Roy 《Bio-Design and Manufacturing》 SCIE EI CAS CSCD 2024年第3期320-357,共38页
The microbiota-gut-brain axis(MGBA)has emerged as a key prospect in the bidirectional communication between two major organ systems:the brain and the gut.Homeostasis between the two organ systems allows the body to fu... The microbiota-gut-brain axis(MGBA)has emerged as a key prospect in the bidirectional communication between two major organ systems:the brain and the gut.Homeostasis between the two organ systems allows the body to function without disease,whereas dysbiosis has long-standing evidence of etiopathological conditions.The most common communication paths are the microbial release of metabolites,soluble neurotransmitters,and immune cells.However,each pathway is intertwined with a complex one.With the emergence of in vitro models and the popularity of three-dimensional(3D)cultures and Transwells,engineering has become easier for the scientific understanding of neurodegenerative diseases.This paper briefly retraces the possible communication pathways between the gut microbiome and the brain.It further elaborates on three major diseases:autism spectrum disorder,Parkinson’s disease,and Alzheimer’s disease,which are prevalent in children and the elderly.These diseases also decrease patients’quality of life.Hence,understanding them more deeply with respect to current advances in in vitro modeling is crucial for understanding the diseases.Remodeling of MGBA in the laboratory uses many molecular technologies and biomaterial advances.Spheroids and organoids provide a more realistic picture of the cell and tissue structure than monolayers.Combining them with the Transwell system offers the advantage of compartmentalizing the two systems(apical and basal)while allowing physical and chemical cues between them.Cutting-edge technologies,such as bioprinting and microfluidic chips,might be the future of in vitro modeling,as they provide dynamicity. 展开更多
关键词 Microbiota-gut-brain axis Neurodegeneration 3D disease model Organoid Transwell system
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Modeling time-dependent mechanical behavior of hard rock considering excavation-induced damage and complex 3D stress states 被引量:1
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作者 Peiyang Yu Xiuli Ding +3 位作者 Peng-Zhi Pan Shuting Miao Zhaofeng Wang Shuling Huang 《Journal of Rock Mechanics and Geotechnical Engineering》 SCIE CSCD 2024年第10期4046-4065,共20页
To investigate the long-term stability of deep rocks,a three-dimensional(3D)time-dependent model that accounts for excavation-induced damage and complex stress state is developed.This model comprises three main compon... To investigate the long-term stability of deep rocks,a three-dimensional(3D)time-dependent model that accounts for excavation-induced damage and complex stress state is developed.This model comprises three main components:a 3D viscoplastic isotropic constitutive relation that considers excavation damage and complex stress state,a quantitative relationship between critical irreversible deformation and complex stress state,and evolution characteristics of strength parameters.The proposed model is implemented in a self-developed numerical code,i.e.CASRock.The reliability of the model is validated through experiments.It is indicated that the time-dependent fracturing potential index(xTFPI)at a given time during the attenuation creep stage shows a negative correlation with the extent of excavationinduced damage.The time-dependent fracturing process of rock demonstrates a distinct interval effect of the intermediate principal stress,thereby highlighting the 3D stress-dependent characteristic of the model.Finally,the influence of excavation-induced damage and intermediate principal stress on the time-dependent fracturing characteristics of the surrounding rocks around the tunnel is discussed. 展开更多
关键词 Hard rock Excavation damage Complex stress state Three-dimensional(3D)time-dependent model
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Modeling and Comprehensive Review of Signaling Storms in 3GPP-Based Mobile Broadband Networks:Causes,Solutions,and Countermeasures
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作者 Muhammad Qasim Khan Fazal Malik +1 位作者 Fahad Alturise Noor Rahman 《Computer Modeling in Engineering & Sciences》 SCIE EI 2025年第1期123-153,共31页
Control signaling is mandatory for the operation and management of all types of communication networks,including the Third Generation Partnership Project(3GPP)mobile broadband networks.However,they consume important a... Control signaling is mandatory for the operation and management of all types of communication networks,including the Third Generation Partnership Project(3GPP)mobile broadband networks.However,they consume important and scarce network resources such as bandwidth and processing power.There have been several reports of these control signaling turning into signaling storms halting network operations and causing the respective Telecom companies big financial losses.This paper draws its motivation from such real network disaster incidents attributed to signaling storms.In this paper,we present a thorough survey of the causes,of the signaling storm problems in 3GPP-based mobile broadband networks and discuss in detail their possible solutions and countermeasures.We provide relevant analytical models to help quantify the effect of the potential causes and benefits of their corresponding solutions.Another important contribution of this paper is the comparison of the possible causes and solutions/countermeasures,concerning their effect on several important network aspects such as architecture,additional signaling,fidelity,etc.,in the form of a table.This paper presents an update and an extension of our earlier conference publication.To our knowledge,no similar survey study exists on the subject. 展开更多
关键词 Signaling storm problems control signaling load analytical modeling 3GPP networks smart devices diameter signaling mobile broadband data access data traffic mobility management signaling network architecture 5G mobile communication
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Integrating remote sensing and 3-PG model to simulate the biomass and carbon stock of Larix olgensis plantation
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作者 Yu Bai Yong Pang Dan Kong 《Forest Ecosystems》 SCIE CSCD 2024年第4期543-555,共13页
Accurate estimations of biomass and its temporal dynamics are crucial for monitoring the carbon cycle in forest ecosystems and assessing forest carbon sequestration potentials.Recent studies have shown that integratin... Accurate estimations of biomass and its temporal dynamics are crucial for monitoring the carbon cycle in forest ecosystems and assessing forest carbon sequestration potentials.Recent studies have shown that integrating process-based models(PBMs)with remote sensing data can enhance simulations from stand to regional scales,significantly improving the ability to simulate forest growth and carbon stock dynamics.However,the utilization of PBMs for large-scale simulation of larch carbon storage distribution is still limited.In this study,we applied the parameterized 3-PG(Physiological Principles Predicting Growth)model across the Mengjiagang Forest Farm(MFF)to make broad-scale predictions of the biomass and carbon stocks of Larix olgensis plantation.The model was used to simulate average diameter at breast height(DBH)and total biomass,which were later validated with a wide range of observation data including sample plot data,forest management inventory data,and airborne laser scanning data.The results showed that the 3-PG model had relatively high accuracy for predicting both DBH and total biomass at stand and regional scale,with determination coefficients ranging from 0.78 to 0.88.Based on the estimation of total biomass,we successfully produced a carbon stock map of the Larix olgensis plantation in MFF with a spatial resolution of 20 m,which helps with relevant management advice.These findings indicate that the integration of 3-PG model and remote sensing data can well predict the biomass and carbon stock at regional and even larger scales.In addition,this integration facilitates the evaluation of forest carbon sequestration capacity and the development of forest management plans. 展开更多
关键词 3-PG model LARCH BIOMASS Carbon stock ALS
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Exploring mechanism of hidden,steep obliquely inclined bedding landslides using a 3DEC model:A case study of the Shanyang landslide in Shaanxi Province,China
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作者 Jia-yun Wang Zi-long Wu +3 位作者 Xiao-ya Shi Long-wei Yang Rui-ping Liu Na Lu 《China Geology》 CAS CSCD 2024年第2期303-314,I0001-I0003,共15页
Catastrophic geological disasters frequently occur on slopes with obliquely inclined bedding structures(also referred to as obliquely inclined bedding slopes),where the apparent dip sliding is not readily visible.This... Catastrophic geological disasters frequently occur on slopes with obliquely inclined bedding structures(also referred to as obliquely inclined bedding slopes),where the apparent dip sliding is not readily visible.This phenomenon has become a focal point in landslide research.Yet,there is a lack of studies on the failure modes and mechanisms of hidden,steep obliquely inclined bedding slopes.This study investigated the Shanyang landslide in Shaanxi Province,China.Using field investigations,laboratory tests of geotechnical parameters,and the 3DEC software,this study developed a numerical model of the landslide to analyze the failure process of such slopes.The findings indicate that the Shanyang landslide primarily crept along a weak interlayer under the action of gravity.The landslide,initially following a dip angle with the support of a stable inclined rock mass,shifted direction under the influence of argillization in the weak interlayer,moving towards the apparent dip angle.The slide resistance effect of the karstic dissolution zone was increasingly significant during this process,with lateral friction being the primary resistance force.A reduction in the lateral friction due to karstic dissolution made the apparent dip sliding characteristics of the Shanyang landslide more pronounced.Notably,deformations such as bending and uplift at the slope’s foot suggest that the main slide resistance shifts from lateral friction within the karstic dissolution zone to the slope foot’s resistance force,leading to the eventual buckling failure of the landslide.This study unveils a novel failure mode of apparent dip creep-buckling in the Shanyang landslide,highlighting the critical role of lateral friction from the karstic dissolution zone in its failure mechanism.These insights offer a valuable reference for mitigating risks and preventing disasters related to obliquely inclined bedding landslides. 展开更多
关键词 LANDSLIDE Steep obliquely inclined bedding slope Failure mode Failure mechanism Apparent dip creep-buckling Lateral friction 3DEC model Landslide numerical model Geological hazards survey engineering
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Multi-scale Modeling and Finite Element Analyses of Thermal Conductivity of 3D C/SiC Composites Fabricating by Flexible-Oriented Woven Process
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作者 Zheng Sun Zhongde Shan +5 位作者 Hao Huang Dong Wang Wang Wang Jiale Liu Chenchen Tan Chaozhong Chen 《Chinese Journal of Mechanical Engineering》 SCIE EI CAS CSCD 2024年第3期275-288,共14页
Thermal conductivity is one of the most significant criterion of three-dimensional carbon fiber-reinforced SiC matrix composites(3D C/SiC).Represent volume element(RVE)models of microscale,void/matrix and mesoscale pr... Thermal conductivity is one of the most significant criterion of three-dimensional carbon fiber-reinforced SiC matrix composites(3D C/SiC).Represent volume element(RVE)models of microscale,void/matrix and mesoscale proposed in this work are used to simulate the thermal conductivity behaviors of the 3D C/SiC composites.An entirely new process is introduced to weave the preform with three-dimensional orthogonal architecture.The 3D steady-state analysis step is created for assessing the thermal conductivity behaviors of the composites by applying periodic temperature boundary conditions.Three RVE models of cuboid,hexagonal and fiber random distribution are respectively developed to comparatively study the influence of fiber package pattern on the thermal conductivities at the microscale.Besides,the effect of void morphology on the thermal conductivity of the matrix is analyzed by the void/matrix models.The prediction results at the mesoscale correspond closely to the experimental values.The effect of the porosities and fiber volume fractions on the thermal conductivities is also taken into consideration.The multi-scale models mentioned in this paper can be used to predict the thermal conductivity behaviors of other composites with complex structures. 展开更多
关键词 3D C/SiC composites Finite element analyses Multi-scale modeling Thermal conductivity
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Tidal modeling based on satellite altimetry observations of TOPEX/ Poseidon, Jason1, Jason2, and Jason3 with high prediction capability: A case study of the Baltic Sea
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作者 Alireza A.Ardalan Asiyeh Hashemifaraz 《Geodesy and Geodynamics》 EI CSCD 2024年第4期404-418,共15页
This research aims to optimize the utilization of long-term sea level data from the TOPEX/Poseidon,Jason1,Jason2,and Jason3 altimetry missions for tidal modeling.We generate a time series of along-track observations a... This research aims to optimize the utilization of long-term sea level data from the TOPEX/Poseidon,Jason1,Jason2,and Jason3 altimetry missions for tidal modeling.We generate a time series of along-track observations and apply a developed method to produce tidal models with specific tidal constituents for each location.Our tidal modeling methodology follows an iterative process:partitioning sea surface height(SSH)observations into analysis/training and prediction/validation parts and ultimately identi-fying the set of tidal constituents that provide the best predictions at each time series location.The study focuses on developing 1256 time series along the altimetry tracks over the Baltic Sea,each with its own set of tidal constituents.Verification of the developed tidal models against the sSH observations within the prediction/validation part reveals mean absolute error(MAE)values ranging from 0.0334 m to 0.1349 m,with an average MAE of 0.089 m.The same validation process is conducted on the FES2014 and EOT20 global tidal models,demonstrating that our tidal model,referred to as BT23(short for Baltic Tide 2023),outperforms both models with an average MAE improvement of 0.0417 m and 0.0346 m,respectively.In addition to providing details on the development of the time series and the tidal modeling procedure,we offer the 1256 along-track time series and their associated tidal models as supplementary materials.We encourage the satellite altimetry community to utilize these resources for further research and applications. 展开更多
关键词 Satellitealtimetry Baltic Sea Ocean tide modeling Jason3 Jason2 Jason1 TOPEx/POSEIDON EOT20 FES2014
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UAV-based transient electromagnetic 3D forward modeling and inversion and analysis of exploration capability
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作者 WEI Laonao LIU Yunhe ZHANG Bo 《Global Geology》 2024年第3期154-166,共13页
Unmanned aerial vehicle transient electromagnetic(UAV-TEM)is a novel airborne exploration method that offers advantages such as low cost,simple operation,high exploration efficiency and suitability for near-surface ex... Unmanned aerial vehicle transient electromagnetic(UAV-TEM)is a novel airborne exploration method that offers advantages such as low cost,simple operation,high exploration efficiency and suitability for near-surface exploration in complex terrain areas.To improve the accuracy of data interpretation in this method,the authors conducted a systematic three-dimensional(3D)forward modeling and inversion of the UAV-TEM.This study utilized the finite element method based on unstructured tetrahedral elements and employed the second-order backward Euler method for time discretization.This allowed for accurate 3D modeling and accounted for the effects of complex terrain.Based on these,the influence characteristics of flight altitudes and the sizes,burial depths,and resistivities of anomalies are compared and analyzed to explore the UAV-TEM systems’exploration capability.Lastly,four typical geoelectrical models of landslides are designed,and the inversion method based on the Gauss-Newton optimization method is used to image the landslide models and analyze the imaging effect of the UAV-TEM method on landslide geohazards.Numerical results showed that UAV-TEM could have better exploration resolution and fine imaging of nearsurface structures,providing important technical support for monitoring,early warning,and preventing landslides and other geological hazards. 展开更多
关键词 UAV 3D forward modeling transient electromagnetic 3D inversion landslide model
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Review of Artificial Intelligence for Oil and Gas Exploration: Convolutional Neural Network Approaches and the U-Net 3D Model
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作者 Weiyan Liu 《Open Journal of Geology》 CAS 2024年第4期578-593,共16页
Deep learning, especially through convolutional neural networks (CNN) such as the U-Net 3D model, has revolutionized fault identification from seismic data, representing a significant leap over traditional methods. Ou... Deep learning, especially through convolutional neural networks (CNN) such as the U-Net 3D model, has revolutionized fault identification from seismic data, representing a significant leap over traditional methods. Our review traces the evolution of CNN, emphasizing the adaptation and capabilities of the U-Net 3D model in automating seismic fault delineation with unprecedented accuracy. We find: 1) The transition from basic neural networks to sophisticated CNN has enabled remarkable advancements in image recognition, which are directly applicable to analyzing seismic data. The U-Net 3D model, with its innovative architecture, exemplifies this progress by providing a method for detailed and accurate fault detection with reduced manual interpretation bias. 2) The U-Net 3D model has demonstrated its superiority over traditional fault identification methods in several key areas: it has enhanced interpretation accuracy, increased operational efficiency, and reduced the subjectivity of manual methods. 3) Despite these achievements, challenges such as the need for effective data preprocessing, acquisition of high-quality annotated datasets, and achieving model generalization across different geological conditions remain. Future research should therefore focus on developing more complex network architectures and innovative training strategies to refine fault identification performance further. Our findings confirm the transformative potential of deep learning, particularly CNN like the U-Net 3D model, in geosciences, advocating for its broader integration to revolutionize geological exploration and seismic analysis. 展开更多
关键词 Deep Learning Convolutional Neural Networks (CNN) Seismic Fault Identification U-Net 3D model Geological Exploration
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La_(1-x)Ce_(x)MnO_(3)-Ba/Al_(2)O_(3)催化剂对NO选择性生成NH_(3)的影响
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作者 宋崇林 郑庆贺 +3 位作者 吕誉 崔立峰 李云强 吕刚 《天津大学学报(自然科学与工程技术版)》 EI CAS CSCD 北大核心 2024年第10期1053-1061,共9页
为了实现碳中和目标,降低内燃机碳排放,稀薄燃烧技术成为了当前重要的研究方向.该技术不仅能提高发动机燃油热效率,还能有效降低CO_(2)排放.但是稀薄燃烧往往会伴随着大量氮氧化物的产生,为了解决该问题,采用LNT-SCR耦合的NO_(x)净化技... 为了实现碳中和目标,降低内燃机碳排放,稀薄燃烧技术成为了当前重要的研究方向.该技术不仅能提高发动机燃油热效率,还能有效降低CO_(2)排放.但是稀薄燃烧往往会伴随着大量氮氧化物的产生,为了解决该问题,采用LNT-SCR耦合的NO_(x)净化技术,此时LNT的作用是将排气中部分NO_(x)转化为NH_(3),为下游的SCR提供还原剂.基于此,制备了LNT催化剂,研究催化剂对NO选择性生成NH_(3)的影响.采用溶胶-凝胶法制备了La_(1-x)Ce_(x)MnO_(3)系列钙钛矿氧化物,并通过分步浸渍法得到了La_(1-x)Ce_(x)MnO_(3)-Ba/Al_(2)O_(3)负载型催化剂.利用XRD、H_(2)-TPR、NO-TPD等表征手段研究了钙钛矿氧化物的晶相结构,以及负载型催化剂的还原特性、NO_(x)吸附-脱附性能等物化性质,并且通过H_(2)选择性催化还原NO实验探究了催化剂掺杂Ce对NO转化成NH_(3)的影响.结果表明,Ce掺杂催化剂具有良好的NH_(3)产物选择性,并且显著提高了NO转化率.温度是NO转化和NH_(3)产物选择性生成的决定性因素,而H_(2)和NO体积比是NO转化和NH_(3)产物选择性生成的关键性因素.其中,La_(0.95)Ce_(0.05)MnO_(3)-Ba/Al_(2)O_(3)在低温下催化活性表现最佳,在350℃、H_(2)和NO体积比为5.0时NH_(3)产物选择性为65%,NO转化率为100%.此外,所制备的La_(1-x)Ce_(x)MnO_(3)都形成了钙钛矿型结构,而且Ce掺杂催化剂的大部分Ce离子可以进入到LaMnO_(3)结构中.在催化剂适量掺杂Ce后,H_(2)消耗总面积增大、还原峰的峰值温度降低,表明掺杂Ce改善了催化剂的还原特性;同时NO吸附和脱附面积增大,表明Ce掺杂改变了催化剂的NO_(x)吸附-脱附性能. 展开更多
关键词 La_(1-x)Ce_(x)MnO_(3)-Ba/Al_(2)O_(3)催化剂 H_(2)选择性催化还原NO NH_(3)产物选择性 NO转化率 晶相结构 还原特性 NO_(x)吸附-脱附
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P2X7R过表达的巨噬细胞MSU晶体诱导痛风炎症反应过程中IL-1β、TNF-α、NLRP3表达观察 被引量:1
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作者 秦丽岩 冀琨 +3 位作者 陈邬锦 张蓓 孙玉萍 李瑞 《山东医药》 CAS 2024年第12期41-45,共5页
目的观察嘌呤能受体P2X配体门控离子通道7的配体(P2X7R)过表达白血病细胞诱导分化的巨噬细胞单钠尿酸盐(MSU)晶体诱导痛风炎症反应过程中NOD样受体家族3(NLRP3)蛋白、IL-1β、TNF-α表达情况。方法取人单核细胞白血病细胞系THP-1,并随... 目的观察嘌呤能受体P2X配体门控离子通道7的配体(P2X7R)过表达白血病细胞诱导分化的巨噬细胞单钠尿酸盐(MSU)晶体诱导痛风炎症反应过程中NOD样受体家族3(NLRP3)蛋白、IL-1β、TNF-α表达情况。方法取人单核细胞白血病细胞系THP-1,并随机分为过表达组、空白组、模型组、对照组;过表达组和空白组分别转染P2X7R过表达质粒、空白载体质粒,转染5 d,将过表达组、空白组、模型组THP-1细胞用100 ng/mL的PMA刺激3 h后分化为巨噬细胞,另将MSU晶体用氢氧化钠溶解配制成浓度为100μg/mL的MSU乳糜状悬液加入培养液中孵育6 h;对照组正常培养。分别采用RT-PCR法和Western blot法测算巨噬细胞P2X7R mRNA、蛋白,ELISA法检测巨噬细胞上清液IL-1β、TNF-α,Western blot法测算巨噬细胞NOD样受体家族3(NLRP3)蛋白。结果与对照组比较,过表达组、空白组、模型组P2X7R mRNA和蛋白相对表达量升高,细胞上清液IL-1β、TNF-α水平升高,细胞NLRP3蛋白相对表达量升高(P均<0.05);与模型组、空白组比较,过表达组P2X7R mRNA、蛋白相对表达量升高,细胞上清液IL-1β、TNF-α水平升高,细胞NLRP3蛋白相对表达量升高(P均<0.05)。结论P2X7R过表达白血病细胞诱导分化的巨噬细胞MSU晶体诱导痛风炎症反应过程中IL-1β、TNF-α、NLRP3表达增加,IL-1β、TNF-α水平升高可能通过激活NLRP3蛋白来实现。 展开更多
关键词 嘌呤能受体P2x配体门控离子通道7的配体 痛风 炎症因子 NOD样受体家族3炎症小体
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Ga_(2-x)Fe_(x)O_(3) 单相多铁性及室温磁电耦合效应的研究进展
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作者 张军 马建春 薛武红 《中国陶瓷》 CAS CSCD 北大核心 2024年第3期1-11,共11页
在单相多铁材料中,利用电场代替磁场来可逆控制磁性这一手段是实现下一代高密度、低功耗磁电多功能器件的理想方法。然而,目前所发现的单相多铁材料大多数都表现出了弱的室温铁电性、铁磁性或者低于室温的磁电工作温度,这严重限制了其... 在单相多铁材料中,利用电场代替磁场来可逆控制磁性这一手段是实现下一代高密度、低功耗磁电多功能器件的理想方法。然而,目前所发现的单相多铁材料大多数都表现出了弱的室温铁电性、铁磁性或者低于室温的磁电工作温度,这严重限制了其在实际生产中的应用。近年来的研究发现,具有强磁电(ME)耦合的第Ⅱ类室温单相多铁Ga_(2-x)Fe_(x)O_(3),其剩余铁电极化强度(Pr)和饱和磁化强度(Ms)在最优的条件下分别可以达到25μC/cm^(2)和1.2μB/f.u.,因而是一种极有可能同时解决上述问题的新型替代材料。首先介绍了单相多铁材料的研究现状以及潜在的应用;然后总结了Ga_(2-x)Fe_(x)O_(3)材料单相多铁性和ME耦合效应的研究历程;最后,围绕Ga_(2-x)Fe_(x)O_(3)未来面临的关键科学问题和挑战进行了详细讨论。 展开更多
关键词 单相多铁性 Ga_(2-x)Fe_(x)O_(3) 铁电性 铁磁性 磁电耦合
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表面基团对Ti_(3)C_(2)T_(x)吸附NO性能影响的第一性原理研究
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作者 邱毅 邹江峰 +4 位作者 马智炜 罗强 刘忠华 陈洋 代逸飞 《材料导报》 EI CAS CSCD 北大核心 2024年第5期138-142,共5页
本工作基于密度泛函理论的第一性原理方法,构建了Ti_(3)C_(2)O_(2)、Ti_(3)C_(2)O1.5(OH)0.5、Ti_(3)C_(2)O(OH)、Ti_(3)C_(2)O1.5F0.5和Ti_(3)C_(2)OF五种模型,从几何结构、电荷转移以及电子性质等方面研究了基团种类和比例对Ti_(3)C_(... 本工作基于密度泛函理论的第一性原理方法,构建了Ti_(3)C_(2)O_(2)、Ti_(3)C_(2)O1.5(OH)0.5、Ti_(3)C_(2)O(OH)、Ti_(3)C_(2)O1.5F0.5和Ti_(3)C_(2)OF五种模型,从几何结构、电荷转移以及电子性质等方面研究了基团种类和比例对Ti_(3)C_(2)T_(x)吸附NO的影响。结果表明:相较于Ti_(3)C_(2)O_(2),含低比例-OH和-F基团的Ti_(3)C_(2)T_(x)对NO的吸附能更大,电荷转移更弱,不利于其探测NO,与实验结果一致;但随着-OH和-F比例的提高,吸附能分别减小和增大,电荷转移分别增强和减弱,表明高比例的-OH有利于Ti_(3)C_(2)T_(x)探测NO,而高比例的-F是不利的;同时,在吸附NO后,Ti_(3)C_(2)O_(2)在费米能级附近的能带极值曲率变小,电子有效质量增大,表明-O基团有利于Ti_(3)C_(2)T_(x)探测NO。在几何弛豫过程中NO分子总是以N原子靠近衬底,吸附距离均较小,而且最近邻原子的电子轨道出现杂化,电子的聚集和消散位于两端,表明最近邻原子间成键较弱且偏离子性。此计算结果可以为Ti_(3)C_(2)T_(x)探测和屏蔽NO提供理论指导,同时为Ti_(3)C_(2)T_(x)的表面改性提供思路。 展开更多
关键词 二维材料 Ti_(3)C_(2)T_(x) NO吸附 密度泛函理论 电子性质
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复方斑蝥胶囊联合旋转容积调强技术对头颈部放疗生存期及血清XRCC1、XRCC3mRNA水平的影响
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作者 任淑惠 王山川 《中华中医药学刊》 CAS 北大核心 2024年第10期40-43,共4页
目的探究复方斑蝥胶囊联合旋转容积调强技术对头颈部放疗患者生存期及血清X线修复交错互补基因1(X-ray Repair Cross Complementing 1,XRCC1)、X线修复交错互补基因3(X-ray Repair Cross Complementing 3,XRCC3)信使核糖核酸(Messenger ... 目的探究复方斑蝥胶囊联合旋转容积调强技术对头颈部放疗患者生存期及血清X线修复交错互补基因1(X-ray Repair Cross Complementing 1,XRCC1)、X线修复交错互补基因3(X-ray Repair Cross Complementing 3,XRCC3)信使核糖核酸(Messenger RNA,mRNA)水平的影响。方法选取2019年6月—2021年7月医院收治的头颈部肿瘤患者97例作为研究对象,根据治疗方法不同进行分组,对照组(48例)采用旋转容积调强技术结合放疗治疗,联合组(49例)在对照组基础上加用复方斑蝥胶囊治疗。观察比较临床疗效、中医证候积分、血清XRCC1及XRCC3mRNA水平、生存期、不良反应。结果联合组客观缓解率高于对照组(P<0.05),但疾病控制率与对照组比较差异无统计学意义(P>0.05)。联合组自汗、恶心呕吐、神疲乏力、食欲差、失眠、头晕眼花等得分低于对照组(P<0.05)。联合组血清XRCC1、XRCC3水平及外周血XRCC1、XRCC3 mRNA表达均低于对照组(P<0.05)。联合组无进展生存期和总生存期均高于对照组(P<0.05)。联合组不良反应发生率(34.69%,17/49)低于对照组不良反应发生率(72.92%,35/48)(P<0.05)。结论复方斑蝥胶囊联合旋转容积调强技术能改善头颈部放疗患者肿瘤客观缓解率,缓解临床症状,降低血清XRCC1、XRCC3mRNA水平,减少不良反应。 展开更多
关键词 复方斑蝥胶囊 旋转容积调强技术 头颈部肿瘤 放疗 生存期 x线修复交错互补基因1 x线修复交错互补基因3
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Ti_(3)C_(2)T_(x)超薄纳米片高效率去除废水中重金属的研究
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作者 于雪荣 姜健 +3 位作者 王秀莉 陈元元 刘惠 陈宁宁 《化学试剂》 CAS 2024年第1期69-75,共7页
通过刻蚀剥离法制备了Ti_(3)C_(2)T_(x)超薄纳米片,探究了其对废水中重金属离子的吸附特性。通过SEM、XRD、AFM、FT-IR、Raman对Ti_(3)C_(2)T_(x)超薄纳米片的形貌和结构进行了表征,通过ICP-MS对处理前后水体中重金属离子的含量进行了... 通过刻蚀剥离法制备了Ti_(3)C_(2)T_(x)超薄纳米片,探究了其对废水中重金属离子的吸附特性。通过SEM、XRD、AFM、FT-IR、Raman对Ti_(3)C_(2)T_(x)超薄纳米片的形貌和结构进行了表征,通过ICP-MS对处理前后水体中重金属离子的含量进行了测试。结果表明剥离的Ti_(3)C_(2)T_(x)超薄纳米片表面含有结构缺陷和羟基,当水体中Cu(Ⅱ)、Pb(Ⅱ)、Cr(Ⅵ)初始浓度为50 mg/L时,47.5 mg Ti_(3)C_(2)T_(x)超薄纳米片对其的去除效率高达90%以上,尤其是对Pb(Ⅱ)的去除效率达到了98.81%,吸附性能远高于大孔树脂、硅藻土和活性炭等常见吸附试剂。在Na(Ⅰ)、Cu(Ⅱ)、Pb(Ⅱ)、Cr(Ⅵ)4种离子共存溶液中,Ti_(3)C_(2)T_(x)超薄纳米片对于Pb(Ⅱ)和Cr(Ⅵ)的去除依然可以达到92%以上。通过动力学和吸附等温拟合,Ti_(3)C_(2)T_(x)纳米片对Pb(Ⅱ)的吸附符合拟二级动力学模型和Langmuir等温模型,对Pb(Ⅱ)的最大吸附量和最低检出限分别为81.7 mg/g和0.0094 mg/L。Ti_(3)C_(2)T_(x)超薄纳米片对重金属离子的吸附特性在化学工业、食品加工的废水处理中具有良好的应用前景。 展开更多
关键词 Ti_(3)C_(2)T_(x) 超薄纳米片 重金属离子 刻蚀剥离 吸附
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基于Bax、Cleaved-Caspase-3蛋白表达探讨大蒜素对膀胱癌细胞增殖、凋亡、迁移、侵袭的作用 被引量:1
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作者 贾磊 张超 +2 位作者 陈俊如 冯岳龙 朱瑞 《中国老年学杂志》 北大核心 2024年第3期637-640,共4页
目的 探讨大蒜素对膀胱癌细胞增殖、凋亡、迁移、侵袭的作用及其机制。方法 以膀胱癌细胞BIU-87为研究对象,以不同浓度(50、75、100 mg/L)大蒜素作用于细胞,测定细胞的增殖能力、凋亡能力、迁移能力及侵袭能力;测定细胞内B细胞淋巴瘤-2... 目的 探讨大蒜素对膀胱癌细胞增殖、凋亡、迁移、侵袭的作用及其机制。方法 以膀胱癌细胞BIU-87为研究对象,以不同浓度(50、75、100 mg/L)大蒜素作用于细胞,测定细胞的增殖能力、凋亡能力、迁移能力及侵袭能力;测定细胞内B细胞淋巴瘤-2相关X蛋白(Bax)、裂解的半胱天冬酶(Cleaved-Caspase)-3蛋白表达水平。结果 不同浓度大蒜素对细胞增殖均有抑制作用,且随着时间和浓度的增长而显著增长(P<0.05)。与空白组相比,不同浓度大蒜素组细胞凋亡率明显较高,且随着浓度的增高而明显增高;侵袭细胞数量明显较低,且随着浓度的增高而明显降低;细胞迁移能力明显较弱,且随着浓度的增高而明显减弱;Bax、Cleaved-Caspase-3蛋白表达明显较高,且随着浓度的升高而明显升高(均P<0.05)。结论 大蒜素可以抑制膀胱癌细胞的增殖、凋亡、迁移和侵袭,其机制可能有调控Bax、Cleaved-Caspase-3蛋白表达有关。 展开更多
关键词 大蒜素 膀胱癌 B细胞淋巴瘤-2相关x蛋白(Bax) 裂解的半胱天冬酶(Cleaved-Caspase)-3
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单层Ti_(3)C_(2)T_(x)/ZnO复合光催化剂的制备及对盐酸四环素的降解应用
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作者 吴方棣 赖丽婷 李学全 《化工新型材料》 CAS CSCD 北大核心 2024年第S01期441-446,共6页
采用二甲基亚砜(DMSO)插层法制备单层Ti_(3)C_(2)T_(x),并用共沉淀法合成了高催化活性的Ti_(3)C_(2)T_(x)/ZnO复合光催化剂。通过X射线粉末衍射(XRD)、扫描电子显微镜(SEM)、X射线光电子能谱(XPS)和紫外-可见漫反射光谱(UV-Vis DRS)等... 采用二甲基亚砜(DMSO)插层法制备单层Ti_(3)C_(2)T_(x),并用共沉淀法合成了高催化活性的Ti_(3)C_(2)T_(x)/ZnO复合光催化剂。通过X射线粉末衍射(XRD)、扫描电子显微镜(SEM)、X射线光电子能谱(XPS)和紫外-可见漫反射光谱(UV-Vis DRS)等手段进行表征。结果表明:ZnO与Ti_(3)C_(2)T_(x)复合后,其光催化性能得到了明显提升,当Ti_(3)C_(2)T_(x)与ZnO复合比例为16%时,其具有最佳的降解效果,在可见光照射2h下对盐酸四环素(TCH)降解率可达91.36%。光催化活性的提高主要由于Ti_(3)C_(2)T_(x)与ZnO表面形成的肖特基势垒有效地抑制了电子空穴的复合,提高了光催化效率。 展开更多
关键词 单层Ti_(3)C_(2)T_(x) ZNO 盐酸四环素 光催化
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Error modeling of 3-RSR parallel robot based on D-H transformation matrix 被引量:3
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作者 李瑞琴 杨斌 《Journal of Measurement Science and Instrumentation》 CAS 2014年第3期53-59,2,共7页
By selecting any one limb of 3-RSR parallel robot as a research object, the paper establishes a position and orienta- tion relationship matrix between the moving platform and the base by means of Denavit-Hartenberg (... By selecting any one limb of 3-RSR parallel robot as a research object, the paper establishes a position and orienta- tion relationship matrix between the moving platform and the base by means of Denavit-Hartenberg (D-H) transformation matrix. The error mapping model is derived from original error to the error of the platform by using matrix differential method. This model contains all geometric original errors of the robot. The nonlinear implicit function relation between po- sition and orientation error of the platform and the original geometric errors is simplified as a linear explicit function rela- tion. The results provide a basis for further studying error analysis and error compensation. 展开更多
关键词 error modeling 3-RSR parallel robot Denavit-Hartenberg (D-H) transformation matrix
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Reliability Analysis of a Mixed Redundant Model in C 3I System 被引量:1
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作者 徐勇 侯朝桢 《Journal of Beijing Institute of Technology》 EI CAS 1999年第3期324-330,共7页
Aim To define a mixed redundant model(MRM), improving the reliability of C 3I system. Methods The model combined the technology characters of two? unit system with one warm stand by unit and function substitute s... Aim To define a mixed redundant model(MRM), improving the reliability of C 3I system. Methods The model combined the technology characters of two? unit system with one warm stand by unit and function substitute system. The reliability and availability equations of MRM were deduced. Results and Conclusion Compared with several other reliability models, it has obvious effect upon improving the system reliability. The effect? cost rate is very high among these models. The model can be used in reliability design, evaluation and check of C 3I system. Only a little attached cost is needed to improve C 3I system reliability effectively. 展开更多
关键词 C 3I system reliability analysis mixed redundant model
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