Objective: To study the relationship between one polymorphism in the promoter of the DNA repair gene XPA and the susceptibility to lung cancer. Methods: Genotypes were determined by the PCR-restriction fragment leng...Objective: To study the relationship between one polymorphism in the promoter of the DNA repair gene XPA and the susceptibility to lung cancer. Methods: Genotypes were determined by the PCR-restriction fragment length polymorphism (PCR-RFLP) method in 310 histologically-confirmed lung cancer cases and 341 age and sex frequency-matched cancer-free controls. Results: The XPA A23G genotype frequencies were 27.1% (AA), 42.9% (AG), and 30.0% (GG) in case patients and21.1% (AA), 5218% (AG), and 26.1% (C-G) in control subjects. Multivariate logistic regression analysis revealed that individuals carrying at least one 23G variant allele (AG + GG genotypes) had a significantly decreased risk for lung cancer (adjusted OR = 0.66; 95 % CI = 0.44- 0.98) compared with the wild-type genotype (23AA). Stratified analysis showed that the protective effect was more evident in subjects with a family history of cancer. Conclusion: These results suggest that the XPA A23G polymorphism may have a role in lung cancer susceptibility in this study population.展开更多
目的:DNA修复能力与肿瘤细胞对铂类药物的敏感性密切相关。该研究利用一种新型单核苷酸多态性(SNP)的检测方法,探讨DNA修复基因XPA的SNP与非小细胞肺癌(NSCLC)患者对顺铂(ciaplatin)或卡铂(carboplatin)为主的化疗方案敏感性...目的:DNA修复能力与肿瘤细胞对铂类药物的敏感性密切相关。该研究利用一种新型单核苷酸多态性(SNP)的检测方法,探讨DNA修复基因XPA的SNP与非小细胞肺癌(NSCLC)患者对顺铂(ciaplatin)或卡铂(carboplatin)为主的化疗方案敏感性的关系。方法:经病理学确诊的晚期NSCLC患者96例,采用顺铂或卡铂为主的方案化疗,2~3个周期后进行临床疗效评价。根据cDNA芯片原理制作一种目的基因芯片,利用双色荧光探针杂交进行咒蹦的A23G多态的基因分型,比较不同基因型对化疗敏感性的影响。组间比较采用χ^2检验,比值比(OR)及其95%可信区间(CI)由logistic回归模型计算。结果:成功进行基因分型,野生型、杂合型和突变型的叠加荧光分别显示为绿色、黄色和红色。携带咒蹦23A/A、A/G和G/G基因型的患者,化疗有效率(完全缓解+部分缓解)分别为35.7%、46.9%和16.7%,差异有显著性统计学意义(P〈0.05);携带G/G基因型患者的化疗失败风险是携带至少1个A等位基因(A/G和A/A基因型)个体的3.57倍;但G等位基因携带者的疗效与A等位基因携带者的相似,差异无统计学意义(30.9% vs 41.7%,P=0.2045)。结论:该芯片检测方法准确、高通量且价格低廉,适用于大规模样本SNP调查;XPA基因多态与NSCLC患者对铂类药物化疗的敏感性相关。展开更多
文摘Objective: To study the relationship between one polymorphism in the promoter of the DNA repair gene XPA and the susceptibility to lung cancer. Methods: Genotypes were determined by the PCR-restriction fragment length polymorphism (PCR-RFLP) method in 310 histologically-confirmed lung cancer cases and 341 age and sex frequency-matched cancer-free controls. Results: The XPA A23G genotype frequencies were 27.1% (AA), 42.9% (AG), and 30.0% (GG) in case patients and21.1% (AA), 5218% (AG), and 26.1% (C-G) in control subjects. Multivariate logistic regression analysis revealed that individuals carrying at least one 23G variant allele (AG + GG genotypes) had a significantly decreased risk for lung cancer (adjusted OR = 0.66; 95 % CI = 0.44- 0.98) compared with the wild-type genotype (23AA). Stratified analysis showed that the protective effect was more evident in subjects with a family history of cancer. Conclusion: These results suggest that the XPA A23G polymorphism may have a role in lung cancer susceptibility in this study population.
文摘目的:DNA修复能力与肿瘤细胞对铂类药物的敏感性密切相关。该研究利用一种新型单核苷酸多态性(SNP)的检测方法,探讨DNA修复基因XPA的SNP与非小细胞肺癌(NSCLC)患者对顺铂(ciaplatin)或卡铂(carboplatin)为主的化疗方案敏感性的关系。方法:经病理学确诊的晚期NSCLC患者96例,采用顺铂或卡铂为主的方案化疗,2~3个周期后进行临床疗效评价。根据cDNA芯片原理制作一种目的基因芯片,利用双色荧光探针杂交进行咒蹦的A23G多态的基因分型,比较不同基因型对化疗敏感性的影响。组间比较采用χ^2检验,比值比(OR)及其95%可信区间(CI)由logistic回归模型计算。结果:成功进行基因分型,野生型、杂合型和突变型的叠加荧光分别显示为绿色、黄色和红色。携带咒蹦23A/A、A/G和G/G基因型的患者,化疗有效率(完全缓解+部分缓解)分别为35.7%、46.9%和16.7%,差异有显著性统计学意义(P〈0.05);携带G/G基因型患者的化疗失败风险是携带至少1个A等位基因(A/G和A/A基因型)个体的3.57倍;但G等位基因携带者的疗效与A等位基因携带者的相似,差异无统计学意义(30.9% vs 41.7%,P=0.2045)。结论:该芯片检测方法准确、高通量且价格低廉,适用于大规模样本SNP调查;XPA基因多态与NSCLC患者对铂类药物化疗的敏感性相关。