Zengcuo’an,located in the southeastern part of Xiamen,is an attractive tourist destination with a blend of tradition and modernity.With a history of over 500 years,it was once a berth(停泊处)for merchant ships during...Zengcuo’an,located in the southeastern part of Xiamen,is an attractive tourist destination with a blend of tradition and modernity.With a history of over 500 years,it was once a berth(停泊处)for merchant ships during the Ming Dynasty.The area played a significant role in maritime trade,providing shelter for ships docking at Zengcuo’an.One of the historical landmarks is the Fort,established in the Qing Dynasty as a naval garrison(海军驻军)and home to a famous Krupp cannon(克虏伯大炮).展开更多
Before moving to Xiamen to work and settle,Scott Patrick and his wife looked at a lot of photos of the city,and Yundang Lake caught their attention.“We saw beautiful pictures of the lake and immediately thought that ...Before moving to Xiamen to work and settle,Scott Patrick and his wife looked at a lot of photos of the city,and Yundang Lake caught their attention.“We saw beautiful pictures of the lake and immediately thought that Xiamen must be a very pleasant city to live in.So,we went there,which proved to be a good choice,”Patrick told ChinAfrica.展开更多
Xiamen University,a beacon of education and culture,invites us to witness the fascinating interplay(相互影响)of movement and stillness within its main gate.As we embark on this journey,we discover a realm(领域)where t...Xiamen University,a beacon of education and culture,invites us to witness the fascinating interplay(相互影响)of movement and stillness within its main gate.As we embark on this journey,we discover a realm(领域)where the passage of time and the transformation of space create an attractive dance,while the enduring essence of knowledge and youthful passion remain ever-present.展开更多
Xiamen,a coastal city famous for its impressive seascape,draws numerous tourists each year.One of the prime locations to appreciate the city’s beauty is the Mountains-to-Sea Trail,an approximately 23-kilometer-long p...Xiamen,a coastal city famous for its impressive seascape,draws numerous tourists each year.One of the prime locations to appreciate the city’s beauty is the Mountains-to-Sea Trail,an approximately 23-kilometer-long pedestrian corridor that offers a unique experience for visitors.展开更多
In a grid investigation, dinoflagellate cysts were collected from sediments in Xiamen Western Harbor in May of 2000, from which five species of cysts were identified: Alexandrium tamarensis, A. minutum, Lingulodinium ...In a grid investigation, dinoflagellate cysts were collected from sediments in Xiamen Western Harbor in May of 2000, from which five species of cysts were identified: Alexandrium tamarensis, A. minutum, Lingulodinium polyedra, Gonyaulax scrippsae and Gymnodinium catenatum, account for about 21% in the species composition. The quanti-tative analysis of the sediments shows that the number of dinoflagellate cysts varies from 51 to 256 cysts/g of sediment, the highest value (>200 cysts/g) being recorded at the stations of the central part of the bay, while the lowest (<100 cysts/g) at the bay mouth. A good linear relationship is found between cyst amount and fine-grained sediments. Complex physiognomies on the seabed, topographty in the bay and weak water exchange are the main factors not only in cyst accumulation but also in their distribution pattern, and have resulted in the difference in cyst densities between the inner bay and the outer bay in the harbor.展开更多
Reproductive rate of a marine planktonic copepod Labidocera euchaeta Giesbrecht in Xiamen Harbor was studied during the period from April 1986 to August 1987. Results showed that rate of egg production (F, eggs/(,d)) ...Reproductive rate of a marine planktonic copepod Labidocera euchaeta Giesbrecht in Xiamen Harbor was studied during the period from April 1986 to August 1987. Results showed that rate of egg production (F, eggs/(,d)) was positively correlated with temperature (T,℃) ) and body weight of spawning female (We,ugC ) as: F=0.0637 (T-7)0.7445Wc. Each month a maximum rate of egg production occurred at one of the five experimental temperatures (10°, 15°, 20°, 25°, and 30℃) near the monthly mean field temperature. The available food density (P, ugC/ml) had significant effect on specific rate of egg production (Fc, %C/d) as :Fc=0.1732(P-0.0179)/P. The gross efficiency of egg production of ca 62% in carbon was independent of food density snd ingestion rate. Observations on in situ egg production and hatching rate demonstrated that this species reproduces continuously all the year round, with summer as its high reproductive season and winter a low one. Ten generations a year is also suggested based on the展开更多
-The development rate of Labidocera euchaeta Giesbrecht in the Xiamen Harbor was studied in laboratory. The results showed that the development times of egg and larval stages ( D ) were significantly correlated with t...-The development rate of Labidocera euchaeta Giesbrecht in the Xiamen Harbor was studied in laboratory. The results showed that the development times of egg and larval stages ( D ) were significantly correlated with temperature ( T ), following the Belehradek function, D = α( T -7. 5)-1.0634. The development time from egg to larval stages and adult, as well as generation time can be predicted from the equation for egg development ( α=411/24) by appropriately multipling the constant (α) with a proportion. The development was not isochronal over the whole larval period. Durations of naupliar stages were short and quasi-isochronal, which favored their non-feeding development. In contrast, durations of copepodid stages (C) were long. CV had the longest duration followed by CI, both of which are considered as the critical stages in the larval development. The acclimation effect of the seasonal temperature on the development rate was also observed.展开更多
The concentration of PAHs in the surface seawater of the western Xiamen Harbor has been analysed in the paper. The PAHs concnetration at 9 station locations varies greatly within the range of 106.1ng/L ~ 4365ng/L. But...The concentration of PAHs in the surface seawater of the western Xiamen Harbor has been analysed in the paper. The PAHs concnetration at 9 station locations varies greatly within the range of 106.1ng/L ~ 4365ng/L. But PAHs pollution is slight as compared with that in similar areas in other parts of the world. PAHs stem mainly from oil pollution, urban and industrial discharges, and combustion of fuels.展开更多
The results from the 38 cruises of phytoplankton surveys in the red tide outburst area in the XiamenHarbor in 1987 indicate 41 species of red tide organisms among 110 species of phytoplankton identified. Species compo...The results from the 38 cruises of phytoplankton surveys in the red tide outburst area in the XiamenHarbor in 1987 indicate 41 species of red tide organisms among 110 species of phytoplankton identified. Species composition was dominant by eurytopic species and warm water species. Species diversity apparently changed with (coldand warm) seasons. There was a high diversity in warm seasons, 63 species in August. Average annual number ofphytoplankton reached 1 119 × 103 ind. /dm3 which was 2 to 2. 6 times that in 2 control monitoring stations. Thenumber of phytoplankton in horizonal distribution displayed an obvious increasing tendency from the harbour mouthto the inner harbor. The phytoplankton number always changed with time, but with 3 peak values in the year round.The daily change of the phytoplankton number with tide exhibited a negative relativity. Moreover phytoplanktonspotes and their number showed a vertical movement during day and night. Futhermore, 10 predominant specieschanged with seasons was noted in the present paper.展开更多
In this paper,the open-sourced computational fluid dynamics software,OpenFOAM~?,is used to study the fluctuation phenomenon of the water body inside a horizontally one-dimensional enclosed harbor basin with constant w...In this paper,the open-sourced computational fluid dynamics software,OpenFOAM~?,is used to study the fluctuation phenomenon of the water body inside a horizontally one-dimensional enclosed harbor basin with constant water depth triggered by falling wedges with various horizontal falling positions,initial falling velocities and masses.Based on both Fourier transfo rm analysis and wavelet spectrum analysis for the time series of the free surface elevations inside the harbor basin,it is found for the first time that the wedge falling inside the harbor can directly trigger harbor resonance.The influences of the three factors(including the horizontal falling position,the initial falling velocity,and the mass)on the response amplitudes of the lowest three resonant modes are also investigated.The results show that when the wedge falls on one of the nodal points of a resonant mode,the mode would be remarkably suppressed.Conversely,when the wedge falls on one of the anti-nodal points of a resonant mode,the mode would be evidently triggered.The initial falling velocity of the wedge mainly has a remarkable effect on the response amplitude of the most significant mode,and the latter shows a gradual increase trend with the increase of the former.While for the other two less significant modes,their response amplitudes fluctuate around certain constant values as the initial falling velocity rises.In general,the response amplitudes of all the lowest three modes are shown to gradually increase with the mass of the wedge.展开更多
Efficient and stable expression of foreign genes in cells and transgenic animals is important for gain-of-function studies and the establishment of bioreactors.Safe harbor loci in the animal genome enable consistent o...Efficient and stable expression of foreign genes in cells and transgenic animals is important for gain-of-function studies and the establishment of bioreactors.Safe harbor loci in the animal genome enable consistent overexpression of foreign genes,without side effects.However,relatively few safe harbor loci are available in pigs,a fact which has impeded the development of multi-transgenic pig research.We report a strategy for efficient transgene knock-in in the endogenous collagen type I alpha 1 chain(COL1A1)gene using the clustered regularly interspaced short palindromic repeats/CRISPR-associated protein 9(CRISPR/Cas9)system.After the knock-in of a 2A peptide-green fluorescence protein(2A-GFP)transgene in the last codon of COL1A1 in multiple porcine cells,including porcine kidney epithelial(PK15),porcine embryonic fibroblast(PEF)and porcine intestinal epithelial(IPI-2I)cells,quantitative PCR(qPCR),Western blotting,RNA-seq and CCK8 assay were performed to assess the safety of COL1A1 locus.The qPCR results showed that the GFP knock-in had no effect(P=0.29,P=0.66 and P=0.20 for PK15,PEF and IPI-2I cells,respectively)on the mRNA expression of COL1A1 gene.Similarly,no significant differences(P=0.64,P=0.48 and P=0.80 for PK15,PEF and IPI-2I cells,respectively)were found between the GFP knock-in and wild type cells by Western blotting.RNA-seq results revealed that the transcriptome of GFP knock-in PEF cells had a significant positive correlation(P<2.2e–16)with that of the wild type cells,indicating that the GFP knock-in did not alter the global expression of endogenous genes.Furthermore,the CCK8 assay showed that the GFP knock-in events had no adverse effects(P_(24)h=0.31,P_(48)h=0.96,P_(72)h=0.24,P_(96)h=0.17,and P_(120)h=0.38)on cell proliferation of PK15 cells.These results indicate that the COL1A1 locus can be used as a safe harbor for foreign genes knock-in into the pig genome and can be broadly applied to farm animal breeding and biomedical model establishment.展开更多
The motion of the moored ship in the harbor is a classical hydrodynamics problem that still faces many challenges in naval operations,such as cargo transfer and ship pairings between a big transport ship and some smal...The motion of the moored ship in the harbor is a classical hydrodynamics problem that still faces many challenges in naval operations,such as cargo transfer and ship pairings between a big transport ship and some small ships.A mathematical model is presented based on the Laplace equation utilizing the porous breakwater to investigate the moored ship motion in a partially absorbing/reflecting harbor.The motion of the moored ship is described with the hydrodynamic forces along the rotational motion(roll,pitch,and yaw)and translational motion(surge,sway,and heave).The efficiency of the numerical method is verified by comparing it with the analytical study of Yu and Chwang(1994)for the porous breakwater,and the moored ship motion is compared with the theoretical and experimental data obtained by Yoo(1998)and Takagi et al.(1993).Further,the current numerical scheme is implemented on the realistic Visakhapatnam Fishing port,India,in order to analyze the hydrodynamic forces on moored ship motion under resonance conditions.The model incorporates some essential strategies such as adding a porous breakwater and utilizing the wave absorber to reduce the port’s resonance.It has been observed that these tactics have a significant impact on the resonance inside the port for safe maritime navigation.Therefore,the current numerical model provides an efficient tool to reduce the resonance within the arbitrarily shaped ports for secure anchoring.展开更多
文摘Zengcuo’an,located in the southeastern part of Xiamen,is an attractive tourist destination with a blend of tradition and modernity.With a history of over 500 years,it was once a berth(停泊处)for merchant ships during the Ming Dynasty.The area played a significant role in maritime trade,providing shelter for ships docking at Zengcuo’an.One of the historical landmarks is the Fort,established in the Qing Dynasty as a naval garrison(海军驻军)and home to a famous Krupp cannon(克虏伯大炮).
文摘Before moving to Xiamen to work and settle,Scott Patrick and his wife looked at a lot of photos of the city,and Yundang Lake caught their attention.“We saw beautiful pictures of the lake and immediately thought that Xiamen must be a very pleasant city to live in.So,we went there,which proved to be a good choice,”Patrick told ChinAfrica.
文摘Xiamen University,a beacon of education and culture,invites us to witness the fascinating interplay(相互影响)of movement and stillness within its main gate.As we embark on this journey,we discover a realm(领域)where the passage of time and the transformation of space create an attractive dance,while the enduring essence of knowledge and youthful passion remain ever-present.
文摘Xiamen,a coastal city famous for its impressive seascape,draws numerous tourists each year.One of the prime locations to appreciate the city’s beauty is the Mountains-to-Sea Trail,an approximately 23-kilometer-long pedestrian corridor that offers a unique experience for visitors.
基金This project was supported by the National Natural Science Foundation of China under contract No.39790111the Natural Science Foundation of Fujian Province.
文摘In a grid investigation, dinoflagellate cysts were collected from sediments in Xiamen Western Harbor in May of 2000, from which five species of cysts were identified: Alexandrium tamarensis, A. minutum, Lingulodinium polyedra, Gonyaulax scrippsae and Gymnodinium catenatum, account for about 21% in the species composition. The quanti-tative analysis of the sediments shows that the number of dinoflagellate cysts varies from 51 to 256 cysts/g of sediment, the highest value (>200 cysts/g) being recorded at the stations of the central part of the bay, while the lowest (<100 cysts/g) at the bay mouth. A good linear relationship is found between cyst amount and fine-grained sediments. Complex physiognomies on the seabed, topographty in the bay and weak water exchange are the main factors not only in cyst accumulation but also in their distribution pattern, and have resulted in the difference in cyst densities between the inner bay and the outer bay in the harbor.
文摘Reproductive rate of a marine planktonic copepod Labidocera euchaeta Giesbrecht in Xiamen Harbor was studied during the period from April 1986 to August 1987. Results showed that rate of egg production (F, eggs/(,d)) was positively correlated with temperature (T,℃) ) and body weight of spawning female (We,ugC ) as: F=0.0637 (T-7)0.7445Wc. Each month a maximum rate of egg production occurred at one of the five experimental temperatures (10°, 15°, 20°, 25°, and 30℃) near the monthly mean field temperature. The available food density (P, ugC/ml) had significant effect on specific rate of egg production (Fc, %C/d) as :Fc=0.1732(P-0.0179)/P. The gross efficiency of egg production of ca 62% in carbon was independent of food density snd ingestion rate. Observations on in situ egg production and hatching rate demonstrated that this species reproduces continuously all the year round, with summer as its high reproductive season and winter a low one. Ten generations a year is also suggested based on the
文摘-The development rate of Labidocera euchaeta Giesbrecht in the Xiamen Harbor was studied in laboratory. The results showed that the development times of egg and larval stages ( D ) were significantly correlated with temperature ( T ), following the Belehradek function, D = α( T -7. 5)-1.0634. The development time from egg to larval stages and adult, as well as generation time can be predicted from the equation for egg development ( α=411/24) by appropriately multipling the constant (α) with a proportion. The development was not isochronal over the whole larval period. Durations of naupliar stages were short and quasi-isochronal, which favored their non-feeding development. In contrast, durations of copepodid stages (C) were long. CV had the longest duration followed by CI, both of which are considered as the critical stages in the larval development. The acclimation effect of the seasonal temperature on the development rate was also observed.
文摘The concentration of PAHs in the surface seawater of the western Xiamen Harbor has been analysed in the paper. The PAHs concnetration at 9 station locations varies greatly within the range of 106.1ng/L ~ 4365ng/L. But PAHs pollution is slight as compared with that in similar areas in other parts of the world. PAHs stem mainly from oil pollution, urban and industrial discharges, and combustion of fuels.
文摘The results from the 38 cruises of phytoplankton surveys in the red tide outburst area in the XiamenHarbor in 1987 indicate 41 species of red tide organisms among 110 species of phytoplankton identified. Species composition was dominant by eurytopic species and warm water species. Species diversity apparently changed with (coldand warm) seasons. There was a high diversity in warm seasons, 63 species in August. Average annual number ofphytoplankton reached 1 119 × 103 ind. /dm3 which was 2 to 2. 6 times that in 2 control monitoring stations. Thenumber of phytoplankton in horizonal distribution displayed an obvious increasing tendency from the harbour mouthto the inner harbor. The phytoplankton number always changed with time, but with 3 peak values in the year round.The daily change of the phytoplankton number with tide exhibited a negative relativity. Moreover phytoplanktonspotes and their number showed a vertical movement during day and night. Futhermore, 10 predominant specieschanged with seasons was noted in the present paper.
基金financially supported by the National Natural Science Foundation of China (Grant No.51911530205)the Natural Science Foundation of Jiangsu Province (Grant No.BK20201455)+5 种基金the Guangdong Basic and Applied Basic Research Foundation (Grant No.2023A1515010890)the Key Laboratory of PortWaterway and Sedimentation Engineering of MOT (Grant No.YK222001-2)the Open Research Fund of Key Laboratory of Water Security Guarantee in Guangdong-Hong Kong-Marco Greater Bay Area of Ministry of Water Resources (Grant No.WSGBAKJ202309)the Qing Lan Project of Jiangsu Universitiesthe Royal Society (Grant No.IECNSFC181321)。
文摘In this paper,the open-sourced computational fluid dynamics software,OpenFOAM~?,is used to study the fluctuation phenomenon of the water body inside a horizontally one-dimensional enclosed harbor basin with constant water depth triggered by falling wedges with various horizontal falling positions,initial falling velocities and masses.Based on both Fourier transfo rm analysis and wavelet spectrum analysis for the time series of the free surface elevations inside the harbor basin,it is found for the first time that the wedge falling inside the harbor can directly trigger harbor resonance.The influences of the three factors(including the horizontal falling position,the initial falling velocity,and the mass)on the response amplitudes of the lowest three resonant modes are also investigated.The results show that when the wedge falls on one of the nodal points of a resonant mode,the mode would be remarkably suppressed.Conversely,when the wedge falls on one of the anti-nodal points of a resonant mode,the mode would be evidently triggered.The initial falling velocity of the wedge mainly has a remarkable effect on the response amplitude of the most significant mode,and the latter shows a gradual increase trend with the increase of the former.While for the other two less significant modes,their response amplitudes fluctuate around certain constant values as the initial falling velocity rises.In general,the response amplitudes of all the lowest three modes are shown to gradually increase with the mass of the wedge.
基金supported by the Major Scientific Research Tasks for Scientific and Technological Innovation Projects of the Chinese Academy of Agricultural Sciences(CAAS-ZDRW202006)the National Transgenic Breeding Project(2018ZX08010-10B)the Agricultural Science and Technology Innovation Program of Chinese Academy of Agricultural Sciences(ASTIP-IAS05).
文摘Efficient and stable expression of foreign genes in cells and transgenic animals is important for gain-of-function studies and the establishment of bioreactors.Safe harbor loci in the animal genome enable consistent overexpression of foreign genes,without side effects.However,relatively few safe harbor loci are available in pigs,a fact which has impeded the development of multi-transgenic pig research.We report a strategy for efficient transgene knock-in in the endogenous collagen type I alpha 1 chain(COL1A1)gene using the clustered regularly interspaced short palindromic repeats/CRISPR-associated protein 9(CRISPR/Cas9)system.After the knock-in of a 2A peptide-green fluorescence protein(2A-GFP)transgene in the last codon of COL1A1 in multiple porcine cells,including porcine kidney epithelial(PK15),porcine embryonic fibroblast(PEF)and porcine intestinal epithelial(IPI-2I)cells,quantitative PCR(qPCR),Western blotting,RNA-seq and CCK8 assay were performed to assess the safety of COL1A1 locus.The qPCR results showed that the GFP knock-in had no effect(P=0.29,P=0.66 and P=0.20 for PK15,PEF and IPI-2I cells,respectively)on the mRNA expression of COL1A1 gene.Similarly,no significant differences(P=0.64,P=0.48 and P=0.80 for PK15,PEF and IPI-2I cells,respectively)were found between the GFP knock-in and wild type cells by Western blotting.RNA-seq results revealed that the transcriptome of GFP knock-in PEF cells had a significant positive correlation(P<2.2e–16)with that of the wild type cells,indicating that the GFP knock-in did not alter the global expression of endogenous genes.Furthermore,the CCK8 assay showed that the GFP knock-in events had no adverse effects(P_(24)h=0.31,P_(48)h=0.96,P_(72)h=0.24,P_(96)h=0.17,and P_(120)h=0.38)on cell proliferation of PK15 cells.These results indicate that the COL1A1 locus can be used as a safe harbor for foreign genes knock-in into the pig genome and can be broadly applied to farm animal breeding and biomedical model establishment.
文摘The motion of the moored ship in the harbor is a classical hydrodynamics problem that still faces many challenges in naval operations,such as cargo transfer and ship pairings between a big transport ship and some small ships.A mathematical model is presented based on the Laplace equation utilizing the porous breakwater to investigate the moored ship motion in a partially absorbing/reflecting harbor.The motion of the moored ship is described with the hydrodynamic forces along the rotational motion(roll,pitch,and yaw)and translational motion(surge,sway,and heave).The efficiency of the numerical method is verified by comparing it with the analytical study of Yu and Chwang(1994)for the porous breakwater,and the moored ship motion is compared with the theoretical and experimental data obtained by Yoo(1998)and Takagi et al.(1993).Further,the current numerical scheme is implemented on the realistic Visakhapatnam Fishing port,India,in order to analyze the hydrodynamic forces on moored ship motion under resonance conditions.The model incorporates some essential strategies such as adding a porous breakwater and utilizing the wave absorber to reduce the port’s resonance.It has been observed that these tactics have a significant impact on the resonance inside the port for safe maritime navigation.Therefore,the current numerical model provides an efficient tool to reduce the resonance within the arbitrarily shaped ports for secure anchoring.