Purity Identification of Xinkui 19 by SSR Marker Technique

In this study, by using Xinkui 19 and its parents as experimental materials, 100 pairs of SSR molecular markers were screened to obtain specific poly- morphic primers, aiming at providing an accurate and efficient method for identifying the purity of Helianthus annuus L. hybrid seeds. According to the experimen- tal results, by using polymorphic SSR primer 455, the amplified bands of female and male parents were 460 and 430 bp, respectively; by using polymorphic SSR primer 478, the amplified bands of female and male parents were 330 and 350 bp, respectively. The identification results of Xinkui 19 hybrids and its parents with SSR marker technique and field cultivation were basically consistent. SSR primers 455 and 478 could be used for rapid and effective identification of the purity of Xinkui 19 hybrid seeds.

油用向日葵新葵19号恢复系654R的选育及利用

[目的]研究油用向日葵恢复系的选育方法。[方法]在现有技术中向日葵恢复系的选择过程比较漫长且结果不明确的情况下,为了改变从杂种一代F1以后连续自交的作法,在早代就开始进行测定其配合力,有目的的选择,方向比较明确,提高选择效率,创造新的优异资源。[结果]选育出油用向日葵新葵19号恢复系654R,该恢复系植株性状好、抗病性好、抗逆性强、配合力高、杂种优势强。[结论]该研究提供了一种油用向日葵恢复系新的选育方法,并对654R进行利用。