Objective：Estradiol （E2） plays an important role in the development of breast cancer.In postmenopausal women,the estrogen can be synthesized via aromatase （CYP19） pathway and steroid-sulfatase （STS） pathway in ...Objective：Estradiol （E2） plays an important role in the development of breast cancer.In postmenopausal women,the estrogen can be synthesized via aromatase （CYP19） pathway and steroid-sulfatase （STS） pathway in peripheral tissues,when the production in ovary has ceased.The objective of our study was to explore the effects of Shu-Gan-Liang-Xue Decoction （SGLXD） on the expressions of CYP19 and STS in estrogen receptor positive breast cancer MCF-7 and T47D cells.Methods：The effects of SGLXD on the cell viability of MCF-7 and T47D were analyzed by MTT assay.By quantitative real-time RT-PCR and Western blot,we evaluated the mRNA and protein expressions of CYP19 and STS in MCF-7 and T47D cells after SGLXD treatment.Results：By MTT assay,the cell viability rates of MCF-7 and T47D were significantly inhibited by SGLXD in a dose-dependent manner,the IC50 values were 40.07 mg/ml for MCF-7 cells and 25.62 mg/ml for T47D cells,respectively.As evidenced by real-time PCR and Western blot,the high concentrations of SGLXD significantly down-regulated the expressions of CYP19 and STS both in the transcript level and the protein level.Conclusion：The results suggest that SGLXD is a potential dual aromatase-sulfatase inhibitor by simultaneously down-regulating the expressions of CYP19 and STS in MCF-7 and T47D cells.展开更多
Xu Chunfu was a famous medical expert in the Ming Dynasty, who authored the Gu Jin Yi Tong Da Quan and the Yi Xue Zhi Nan Jie Jing Liu Shu. This paper uses the Xu family genealogy, literature catalog, historical recor...Xu Chunfu was a famous medical expert in the Ming Dynasty, who authored the Gu Jin Yi Tong Da Quan and the Yi Xue Zhi Nan Jie Jing Liu Shu. This paper uses the Xu family genealogy, literature catalog, historical records to study the Xu family’s life and deeds. Xu Chunfu was born in the second year of the Jiajing reign of the Ming Dynasty (1523) and passed away in the 24th year of the Wanli reign of the Ming Dynasty (1596). He served as an official in the Tai Hospital for about 20 years. His work Gu Jin Yi Tong Da Quan was written around the 43rd year of the Jiajing reign of the Ming Dynasty (1556), with a lower limit of the fifth year of the Longqing reign of the Ming Dynasty (1571), and the initial engraving time was not earlier than the eighth year of the Wanli reign of the Ming Dynasty (1580). This paper corrects the shortcomings and errors in previous research, and provides a comprehensive and systematic examination of Xu Chunfu’s life and works.展开更多
基金supported by the National Natural Science Foundation of China (No. 200930973906)
文摘Objective：Estradiol （E2） plays an important role in the development of breast cancer.In postmenopausal women,the estrogen can be synthesized via aromatase （CYP19） pathway and steroid-sulfatase （STS） pathway in peripheral tissues,when the production in ovary has ceased.The objective of our study was to explore the effects of Shu-Gan-Liang-Xue Decoction （SGLXD） on the expressions of CYP19 and STS in estrogen receptor positive breast cancer MCF-7 and T47D cells.Methods：The effects of SGLXD on the cell viability of MCF-7 and T47D were analyzed by MTT assay.By quantitative real-time RT-PCR and Western blot,we evaluated the mRNA and protein expressions of CYP19 and STS in MCF-7 and T47D cells after SGLXD treatment.Results：By MTT assay,the cell viability rates of MCF-7 and T47D were significantly inhibited by SGLXD in a dose-dependent manner,the IC50 values were 40.07 mg/ml for MCF-7 cells and 25.62 mg/ml for T47D cells,respectively.As evidenced by real-time PCR and Western blot,the high concentrations of SGLXD significantly down-regulated the expressions of CYP19 and STS both in the transcript level and the protein level.Conclusion：The results suggest that SGLXD is a potential dual aromatase-sulfatase inhibitor by simultaneously down-regulating the expressions of CYP19 and STS in MCF-7 and T47D cells.
基金the 2020 National Social Science Foundation Major Project(20&ZD222)Open Project of Key Laboratory of Xin’an Medical Education in 2022,“Research on the Active Utilization of Intangible Cultural Heritage Resources in the Gu Jin Yi Tong Da Quan"(2022xayx01)2022 Anhui Provincial Central Finance Traditional Chinese Medicine Special Fund“North Huatuo,South Xin’an”Inheritance and Innovation Project:Systematic Compilation and Audiovisual Protection Research of Xin’an Medical Traditional Medicine Intangible Cultural Heritage Project(2022BHTNXA02).
文摘Xu Chunfu was a famous medical expert in the Ming Dynasty, who authored the Gu Jin Yi Tong Da Quan and the Yi Xue Zhi Nan Jie Jing Liu Shu. This paper uses the Xu family genealogy, literature catalog, historical records to study the Xu family’s life and deeds. Xu Chunfu was born in the second year of the Jiajing reign of the Ming Dynasty (1523) and passed away in the 24th year of the Wanli reign of the Ming Dynasty (1596). He served as an official in the Tai Hospital for about 20 years. His work Gu Jin Yi Tong Da Quan was written around the 43rd year of the Jiajing reign of the Ming Dynasty (1556), with a lower limit of the fifth year of the Longqing reign of the Ming Dynasty (1571), and the initial engraving time was not earlier than the eighth year of the Wanli reign of the Ming Dynasty (1580). This paper corrects the shortcomings and errors in previous research, and provides a comprehensive and systematic examination of Xu Chunfu’s life and works.
