Fine-mapping and primary analysis of candidate genes associated with seed coat color in mung bean(Vigna radiata L.)

Seed coat color affects the appearance and commodity quality of mung beans(Vigna radiata L.).The substances that affect mung bean seed coat color are mainly flavonoids,which have important medicinal value.Mapping the seed coat color gene in mung beans would facilitate the development of new varieties and improve their value.In this study,an F2 mapping population consisting of 546 plants was constructed using Jilv9(black seed coat)and BIS9805(green seed coat).Using bulk segregated analysis(BSA)sequencing and kompetitive allele-specific PCR(KASP)markers,the candidate region related to seed coat color was finally narrowed to 0.66 Mb on chromosome(Chr.)4 and included eight candidate genes.Combined transcriptome and metabolome analyses showed that three of the eight candidate genes(LOC106758748,LOC106758747,and LOC106759075)were differentially expressed,which may have caused the differences in flavonoid metabolite content between Jilv9 and BIS9805.These findings can provide a research basis for cloning the genes related to seed coat color and accelerate molecular markerassisted selection breeding in mung beans.

Domestication of Marama Bean in Arid Namibia: Challenges and Opportunities in a Climate Changing Agroecology

Tylosema esculentum (Burch.) A. Schreib. (Marama bean), referred to as marama in sections of this article, is an obligate outcrossing native plant with a yield potential of 2 ton/hectare which grows naturally in the deep sandy soils of the Kalahari Desert. It has adapted to the low precipitation levels in that agro-ecosystem. Marama serves as a staple food for the San and Bantus in that area. In Namibia, in the past you could find wild stands of marama in the Khomas region, Omaheke region, and the Otjozondjupa region without must struggle. It is renowned for its brown seeds, which are rich in high-quality oils and proteins. The tuberous root contains a significant amount of starch. The objective of domesticating orphaned marama is to provide farmers in this climate change-prone region with a viable alternative for food and nutrition security. This program, initiated in 2008 with an open-minded mindset, required swift implementation using harsh and occasionally unconventional methods. To introduce indigenous tools for resource-poor farmers, the domestication program prioritized the utilization of farmer-participatory methodologies. It was crucial to integrate old and new approaches to ensure learning from past and present experiences, leading to innovative solutions. There is little research and development of native crops in Africa because most of the currently cultivates crops were brought for use from abroad. Only a few numbers of indeginous African crops can be named. The arid Kalahari region, susceptible to climate change, necessitates the revival of indigenous crops like marama, which are resilient and well-adapted to the region’s conditions and have thrived for centuries. In many discussions regarding the health and nutrition of Africa, the recommendation to consume traditional foods to avoid exposure to modern foods, which may not be genetically compatible, is frequently emphasized. Regardless of their validity, these opinions must be acknowledged, and steps need to be taken to ensure a positive legacy for future generations. However, this chapter will address the limitations and challenges that exist in this regard. This article will summarize the progress made in the domestication program of the marama bean in Namibia thus far. Furthermore, this article will highlight the challenges that have been faced during the domestication journey for marama bean and other orphaned crops. The domestication program commenced with a broad germplasm collection, characterization, and preselection for breeding. Crop selection in this program was influenced by climate change-related concerns of shorter and uncertain rain seasons, and recurrent droughts. Selection included but was not limited to identifying marama genotypes with superior characteristics, early germination and many seeds per pod were among some of the identified and selected characteristics. The Namibia University of Science and Technology (NUST) has compiled a list of potential marama bean varieties and is currently testing marama seeds in anticipation of their introduction as a new crop alternative with good adaptation to the effects of climate change, since conventional crops like maize underperform due to persistent droughts. Marama bean, if properly developed, holds significant potential to address issues of hunger and malnutrition in arid regions of Southern Africa and other similar territories. The findings presented here are the result of ongoing field research and experiments conducted at multiple sites using superior marama bean varieties.

Glycemic Responses of Food Formulations Based on White Rice and White Bean

The management of diabetes mellitus is based on lifestyle and dietary measures suggesting the consumption of foods with a low glycemic index. The present study was conducted in order to propose food formulas based on white rice and white beans resulting in a lower glycemic response. For this, food formulations based on these foods were first made, in particular the formulation [75% white rice + 25% white beans] and the formulation [50% rice + 50% white beans]. Then, the biochemical composition of white rice, white bean and these two formulations was determined by the usual methods. Finally, their glycemic responses over 120 minutes were studied by the FAO/ WHO 1998 method. The results showed that white rice [100% white rice] had the highest carbohydrate content (54.61%) followed respectively by the formulation [75% white rice + 25% white bean] (45.69%), the white bean [100% white bean] (29.1%) and the formulation [50% white rice + 50% white bean] (26.16%). In terms of glycemic responses, the formulation [50% white rice + 50% white beans] presented the best evolution with a lower peak (6.041 mmol/L) observed at T45min and a lower postprandial glycemia (4.872 mmol/ L) at T120min. An increase in the proportion of beans is therefore recommended as it may suggest a beneficial metabolic effect.

Genetic dissection of yield-related traits in response to drought stress in common bean

Drought stress severely impairs common bean production.For facilitating drought-resistance breeding in common bean,molecular markers were identified in a genome-wide level marker–trait association study.A panel of 210 common bean accessions showed large variation in 11 agronomic traits at the adult stage(plant height,pod number per plant,seed number per pod,seed number per plant,seed yield per plant,pod length,harvest index,pod harvest index,days to maturity,hundred-seed weight,and seed yield)under two water conditions.The coefficient of variation ranged from 6.21% for pod harvest index to51.00% for seed number per plant under well-watered conditions,and from 4.05% for days to maturity to 40.72% for seed number per plant under drought stress.In a genome-wide association study,119quantitative-trait loci were associated with drought resistance,including 41 adjacent to known loci.Among these loci,12 were found to be associated with at least two traits.Three major loci were identified at Pv01 and Pv02.A set of candidate genes were found that encode MYBs,AREBs,WKRYs,and protein kinases.These results reveal promising alleles that control drought resistance,shedding light on the genetic basis of drought resistance and accelerating future efforts for drought resistance improvement in common bean.

The molecular mechanism of WRINKLED1 transcription factor regulating oil accumulation in developing seeds of castor bean

The transcription factor WRINKLED1(WRI1),a member of AP2 gene family that contain typical AP2 domains,has been considered as a master regulator regulating oil biosynthesis in oilseeds.However,the regulatory mechanism of RcWRI1 in regulating oil accumulation during seed development has not been clearly addressed.Castor bean(Ricinus communis)is one of the most important non-edible oil crops and its seed oils are rich in hydroxy fatty acids,widely applied in industry.In this study,based on castor bean reference genome,three RcWRIs genes(RcWRI1,RcWRI2 and RcWRI3)were identified and the expressed association of RcWRI1 with oil accumulation were determined.Heterologous transformation of RcWRI1 significantly increased oil content in tobacco leaf,confirming that RcWRI1 activate lipid biosynthesis pathway.Using DNA Affinity Purification sequencing(DAP-seq)technology,we confirmed RcWRI1 binding with Transcription Start Site of genes and identified 7961 WRI1-binding candidate genes.Functionally,these identified genes were mainly involved in diverse metabolism pathways(including lipid biosynthesis).Three cis-elements AW-box([CnTnG](n)7[CG])and AW-boxes like([GnAnC](n)6[GC]/[GnAnC](n)7[G])bound with RcWRI1 were identified.Co-expression network analysis of RcWRI1 further found that RcWRI1 might be widely involved in biosynthesis of storage materials during seed development.In particular,yeast one hybrid experiments found that both AP2 domains within RcWRI1 were required in binding targeted genes.These results not only provide new evidence to understand the regulatory mechanism of RcWRI1 in regulation of oil accumulation during castor bean seed development,but also give candidate gene resource for subsequent genetic improvement toward increasing oil content in oilseed crops.

Proteomic Study of Differentially Expressed Proteins in Seeds between Parents and Offspring of Castor Bean(Ricinus communis L.)

Castor bean(Ricinus communis L.),is one of the top 10 oilseed crops in the world and,therefore,of high economic value.Hybridization is one of the most effective ways to breed new varieties with high yield,high oil content,and better stress resistance.Therefore,prediction of desired traits in castor hybrid offspring is particularly important.In this study,proteomic analysis was performed to identify differentially expressed proteins(DEPs)in seeds between castor hybrid offspring and their female(Lm female line aLmAB2)and male parents(CSR·181).Among the DEPs upregulated in the seeds of hybrid offspring,the majority were related to seed yield and stress tolerance,while some were related to oil synthesis and fatty acid synthesis and metabolism in seeds.In other words,the hybrid offspring showed heterosis for seed yield,stress tolerance,oil synthesis,and fatty acid synthesis and metabolism when compared with their parents.Further,real-time quantitative polymerase chain reaction assays were performed on 12 genes encoding DEPs involved in oil synthesis,pollen abortion,yield,and stress tolerance of seeds.The results showed that the expression levels of the 12 genes were consistent with those of the DEPs.

Effects of Variety and Planting Density on Mung Bean Eco-Physiology and Yield in the Southeastern US

Mung bean (Vigna radiata L. Wilczek.) is a warm-season, C3 pulse crop of the legume family that has been widely cultivated in Asian countries. As the demand for mung bean continues to increase in the United States, the ecophysiology, growth, and yield of mung bean varieties in the southeastern US need to be assessed. A field experiment was conducted at the Agricultural Research and Education Center of Tennessee State University to investigate the effects of four varieties (OK2000, Berken, TSU-1, AAMU-1) and three planting densities (5, 10, and 15 cm spacing) on the ecophysiology and yield of mung bean. Results showed that the relative chlorophyll content, plant height, pod dry biomass, pod number, crop yield, and harvest index significantly varied among the varieties. Density only influenced transpiration, relative chlorophyll content, and plant dry biomass. OK2000 had 101.0% more pods per plant and a 42.4% higher harvest index and produced a 45.3% higher yield than other varieties, but no significant difference in yield was found among the other three varieties. This study demonstrated that the mung bean variety OK2000 with a high yield would be ideal for commercial production in the southeastern US.

Drought Tolerance in Mung Bean is Associated with the Genotypic Divergence, Regulation of Proline, Photosynthetic Pigment and Water Relation

Drought is one of the critical conditions for the growth and productivity of many crops including mung bean(Vigna radiata L.Wilczek).Screening of genotypes for variations is one of the suitable strategies for evaluating crop adaptability and global food security.In this context,the study investigated the physiological and biochemical responses of four drought tolerant(BARI Mung-8,BMX-08010-2,BMX-010015,BMX-08009-7),and four drought sensitive(BARI Mung-1,BARI Mung-3,BU Mung-4,BMX-05001)mung bean genotypes under wellwatered(WW)and water deficit(WD)conditions.The WW treatment maintained sufficient soil moisture(22%±0.5%,i.e.,30%deficit of available water)by regularly supplying water.Whereas,the WD treatment was maintained throughout the growing period,and water was applied when the wilting symptom appeared.The drought tolerant(DT)genotypes BARI Mung-8,BMX-08010-2,BMX-010015,BMX-08009-7 showed a high level of proline accumulation(2.52–5.99 mg g^(−1) FW),photosynthetic pigment(total chlorophyll 2.96–3.27 mg g^(−1) FW at flowering stage,and 1.62–2.38 mg g^(−1) FW at pod developing stage),plant water relation attributes including relative water content(RWC)(82%–84%),water retention capacity(WRC)(12–14)as well as lower water saturation deficit(WSD)(19%–23%),and water uptake capacity(WUC)(2.58–2.89)under WD condition,which provided consequently higher relative seed yield.These indicate that the tolerant genotypes gained better physiobiochemical attributes and adaptability in response to drought conditions.Furthermore,the genotype BMX-08010-2 showed superiority in terms of those physio-biochemical traits,susceptibility index(SSI)and stress tolerance index(STI)to other genotypes.Based on the physiological and biochemical responses,the BMX-08010-2 was found to be a suitable genotype for sustaining yield under drought stress,and subsequently,it could be recommended for crop improvement through hybridization programs.In addition,the identified traits can be used as markers to identify tolerant genotypes for drought-prone areas.

Yield, Growth and Vegetative Development Parameters Response of Dry Beans to Organic and Inorganic Fertilizers and Biofertilizer

As part of the promotion of common bean cultivation, fertilization methods will have to be proposed to growers. The aim of this study is therefore to develop a technical itinerary for dry bean fertilization. To this end, different types of chemical and organic fertilizers were evaluated on three dry bean varieties (HARI25/GHA19, HARI35/GHA19 and HARI36/GUI21). Seven (7) doses of chemical and organic fertilizers were used, including two controls (D0 with no fertilizer and D1, the reference dose using NPK base and cover fertilizers in the form of urea). The fertilization trial was set up as a Split-Plot design, with variety as the primary factor and dose as the secondary factor. The experiment was repeated three (3) times. The results showed that vegetative development parameters and fruit set rate varied according to the variety studied. For yield and its components, the treatments had a significant effect. Indeed, the response of varieties to fertilizers was specific. For each variety used, the optimum yield was obtained with a different treatment, thus highlighting the genotype effect of the dry bean varieties studied. Among the treatments tested, D4 (5 t organic fertilizer/ha) performed best in all three varieties, generating yield increases of 20%, 46% and 91% respectively.

Integrated Transcriptome and Small RNA Sequencing Analyses Reveals Insights into the Molecular Mechanism of Seed Germination in Mung Bean

During the life cycle of a plant,seed germination is crucial.Upon ingestion of water,the dry seeds resumed energy metabolism and cellular repair.To dissect the complex mechanisms at the very beginning of seed germination,two approaches including transcriptome and small RNA sequencing were conducted during the water imbibition process of mung bean seeds compared with dry seed.The transcriptome sequencing analysis identified 10,108 differentially expressed genes(DEGs)between dry and imbibed mung bean seeds.KEGG enrichment analyses demonstrated numerous DEGs involved in hormone signaling pathways,carbohydrate,and energy metabolism.Out of the total DEGs,129 genes were investigated to involve in abscisic acid,gibberellin,and ethylene pathways,with 56 genes involved in glycolysis and TCA cycle.Through the analysis of small RNA sequencing among different samples,there were 284 miRNAs found in the imbibed and dried seeds of mung bean containing 213 known and 71 novel miRNAs.From comparison between the two types of seeds,51 miRNAs were differentially expressed.The integrated analyses of transcriptome and miRNAome data showed that 23 DEGs are the putative target genes of 8 miRNAs including miR156,miR171b-3p,miR166e-3p,miR169-1,etc.QRT-PCR was used to validate several DEGs and miRNAs.The data suggested that the seed imbibition process is regulated by a variety of DEGs and microRNAs.Meanwhile,we hope to gain a deeper insight into how mung bean seeds germinate in order to optimize seed growth.

Construction of SNP genetic maps based on targeted next-generation sequencing and QTL mapping of vital agronomic traits in faba bean(Vicia faba L.)

Owing to the limitation of a large genome size(~13 Gb),the genetic and gene mapping studies on faba bean(Vicia faba L.)are lagging far behind those for other legumes.In this study,we selected three purified faba bean lines(Yundou 8137,H0003712,and H000572)as parents and constructed two F2 populations.These two F2 populations,namely 167 F2 plants in Pop1(Yundou 8137×H0003712)and 204 F2 plants in Pop2(H000572×Yundou 8137),were genotyped using a targeted next-generation sequencing(TNGS)genotyping platform,and two high-density single nucleotide polymorphisms(SNP)genetic linkage maps of faba bean were constructed.The map constructed from Pop1 contained 5103 SNPs with a length of 1333.31 cM and an average marker density of 0.26 cM.The map constructed from Pop2 contained 1904 SNPs with a greater length of 1610.61 cM.In these two F2 populations,QTL mapping identified 98 QTLs for 14 agronomic traits related to the flowers,pods,plant types and grains.The two maps were then merged into an integrated genetic linkage map containing 6895 SNPs,with a length of 3324.48 cM.These results not only lay the foundation for fine mapping and map-based cloning of related genes,but can also accelerate the molecular marker-assisted breeding of faba bean.

Isolation and Authentication of Local Rhizobia Nodulating Common Bean (Phaseolus vulgaris L.) in Different Agro-Ecological Zones of Côte d’Ivoire

Legumes such as common bean (Phaseolus vulgaris L.) have been introduced into cropping systems for sustainable soil management. Consequently, the loss of fertility of the latter remains a major constraint to bean production because this legume is rarely fertilized, yet it is considered to be a poor nitrogen fixer in the absence of inoculation. To overcome this, this study was undertaken with the objective of seeking efficient local rhizobia in order to propose a bean inoculum formulation. To do this, soil samples taken from twelve localities in the Centre, North and West areas of C?te d’Ivoire were used to trap bean nodulating rhizobia. The ROBA1 bean accession used was sown in pots containing the sampled soils. Seedlings were uprooted at the start of flowering and nodulation was assessed. The isolates obtained were purified and then characterized phenotypically. The infectivity and symbiotic efficacy of these isolates were determined in vitro by the authentication test in which the purified isolates were reinoculated to their original host plant. A total of 24 rhizobium isolates were obtained from the soils of six localities. During morphological characterization, the isolates showed typical characteristics of Rhizobium. With the exception of RPC501, RPC505 and RPC522, all isolates were authenticated and able to nodulate the host plant in controlled culture. Isolates RPC502, RPC507, and RPC508 were effective and significantly increased (P < 0.05) nodule number and weight, height, and plant biomass. This study has, therefore, revealed the presence of effective local rhizobia in Ivorian soils and capable of nodulating common beans. A genetic characterization of efficient rhizobia identified after experimentation in different environmental conditions should be considered before being recommended as bean rhizobia inoculant.

Cloning and Functional Validation of Mung Bean VrPR Gene

For the purpose of functional validation,the mung bean(Vigna radiata)VrPR gene was cloned and overexpressed in Arabidopsis thaliana.Thefindings revealed that the ORF of VrPR contained 1200 bp,in which 399 amino acids were encoded.Bioinformatics analysis showed that the VrPR protein belonged to the NADB Rossmann superfamily,which was one of the non-transmembrane hydrophilic proteins.VrPR was assumed to have 44 amino acid phosphorylation sites and be contained in chloroplasts.The VrPR secondary structure comprised of random coil,αhelix,βangle,and extended chain,all of which were quite compatible with the anticipated tertiary structure.Moreover,analysis of the phylogenetic tree indicated that the soybean PR(Glyma.12G222200)and VrPR were closely related.Furthermore,chlorophyll content in leaves is markedly increased in Arabidopsis when VrPR is overexpressed.Ourfindings will serve as a reference for more functional studies on the PR genes in mung bean.

Wastewater Use for Corn and Bean Crops

The global demand for water for agricultural use is continuously increasing worldwide as a result of population growth and food supply, approximately 1% per year since the 1980s. Irrigated agriculture represents 20% of the total cultivated area and contributes 40% of the total food production worldwide. Competition for water resources is particularly much needed in arid and semi-arid regions, where irrigation is essential for successful agriculture. This leads to the need to treat larger amounts of wastewater, in order to compensate for the lack of rainwater to irrigate crops and thus favor the environment and the economic development of farmers. In Mexico there are two highly consumed food products, they are: corn and beans, a fundamental part of the Mexican people diet. It can be said that corn is basic in the diet of Mexicans, it is consumed mainly as “tortilla” and in “tamales”, also in some stews;there is a variety called fodder corn to feed cattle. Beans are also an essential part of the Mexican diet for its nutritional value and pleasant flavor. To solve the challenges of the future in the supply of water for agriculture, it is necessary to reconsider that not all farmers can afford irrigation systems and depend on rainwater, so the use of treated wastewater in treatment plants can be useful.

低氧联合酸胁迫红小豆/绿豆萌发富集GABA及富含GABA芽豆复配米饭的工艺优化

为了研究低氧联合酸胁迫对红小豆和绿豆GABA富集的作用,采用单因素对萌发时间、萌发温度、低氧时间和L-谷氨酸浓度进行考察,在确定高GABA芽豆的胁迫条件基础上,将富含GABA红小豆、绿豆与大米进行复配,利用D-混料设计优化芽豆米饭配方工艺。结果显示,低氧联合酸胁迫对红小豆、绿豆富集GABA有积极促进作用,在萌发时间48 h、萌发温度40℃、低氧时间15 h和L-谷氨酸浓度2.5 mg/mL条件下,萌发红小豆中GABA高达158.32±3.24 mg/100 g。绿豆胁迫条件为萌发时间24 h、萌发温度35℃、低氧时间15 h和L-谷氨酸浓度2.5 mg/mL时,其中GABA含量最高为141.57±4.35 mg/100 g。在此基础上,通过D-混料设计优化确定了复配芽豆米饭的最佳配方为:大米76%、萌发绿豆11%、萌发红小豆13%,此条件下,芽豆米饭GABA含量为23.73±1.03 mg/100 g,感官评分均值为88.76±2.47,制得的芽豆米饭口感、色泽、香味均在可接受范围内,且积累了GABA活性成分,提升了芽豆米饭的营养及功能特性,为进一步开发杂粮复配米饭提供理论参考。

富含γ-氨基丁酸的萌发红小豆对T2DM小鼠肠道菌群的影响

为了研究富含GABA萌发红小豆对2型糖尿病(Diabetes Mellitus Type 2,T2DM)小鼠血糖水平及肠道菌群的影响,采用C57BL/6J小鼠为研究对象,通过高脂膳食+链脲菌素(Streptozocin,STZ)注射构建T2DM模型,选择不同剂量的富含GABA萌发红小豆对T2DM小鼠连续膳食干预6周,并利用16S rRNA测序技术对T2DM小鼠盲肠内容物的菌群结构和分布进行鉴定。结果显示:不同剂量富含GABA红小豆膳食干预可使T2DM小鼠FBG值明显下降,其中高剂量富含GABA红小豆(TF3)组FBG值为8.36±0.78 mmol/L,相比模型组(M)下降54.09%,干预效果最好。此外,TF3膳食可引起T2DM小鼠肠道菌群丰度发生显著(P<0.05)改变,门水平上Firmicutes丰度为35.96%,比M模型组下降53.17%,并可显著上调Bacteroidetes、Verrucomicrobia的菌群丰度(P<0.05)。表明TF3膳食改善糖脂代谢与Bacteroidetes、Verrucomicrobia优势菌丰度呈正相关,暗示高剂量富含GABA红小豆膳食可通过增加有益菌来缓解T2DM小鼠高血糖症状,为进一步解释T2DM与肠道菌群的关系提供参考。

绿豆糜/亲水胶体复合物流变学性质及在三维打印吞咽障碍食品中的应用研究

该文以绿豆糜为食物基质,系统研究了黄原胶、阿拉伯胶、κ-卡拉胶、高酯果胶、魔芋葡甘聚糖5种亲水胶体(添加量为绿豆干重的1%)对绿豆糜流变学性质、3D打印性及吞咽性的影响规律。结果表明,添加亲水胶体可有效提升产品的流动应力、剪切稀释性、触变性(除魔芋葡甘聚糖)、蠕变恢复性和凝胶强度,并且减少流动水含量。纯绿豆糜及添加高酯果胶、κ-卡拉胶和阿拉伯胶的绿豆糜打印效果不佳。添加黄原胶和魔芋葡甘聚糖的绿豆糜打印精确度高。吞咽障碍测试结果表明,除纯绿豆糜外,添加亲水胶体的绿豆糜均达到吞咽障碍食品5级-细馅型标准。该研究对深入理解物料流变学特性与3D打印之间的内在关联、丰富吞咽障碍食品的研发手段具有重要价值。

不同叶面肥对稻茬蚕豆产量和品质的影响

为探讨蚕豆高效栽培技术,研究了6种不同叶面肥对稻茬蚕豆产量和品质的影响。结果表明:与对照(CK)相比,赤·吲乙·芸苔(T1)、生根粉(T2)、含氨基酸水溶肥(T4)、含腐殖酸水溶肥(T5)、糖醇螯合硅肥(T6)处理的蚕豆株高均显著增加;T1、复硝酚钠(T3)、T4、T5处理的蚕豆始荚高显著增加;T1处理的蚕豆大分枝节数显著增加;T2、T4、T5处理的蚕豆籽粒显著增大,T5处理的蚕豆籽粒百粒重显著提高13.39%。从产量上看,T5处理的稻茬蚕豆荚和籽粒产量分别较CK显著增加63.96%和58.97%。从品质指标来看,T4处理的蚕豆籽粒蛋白质含量较CK显著增加8.33%;T2处理的籽粒脂肪含量较CK显著增加15.73%。合理喷施适宜的叶面肥有助于提高稻茬蚕豆产量和部分品质指标,可在稻茬蚕豆栽培技术中应用。

金冠豆角籽粒淀粉组成及性质研究

为了明确金冠豆角淀粉的基本性质,本文以提取盐溶蛋白后剩余的金冠豆角籽粒豆渣为原料,采用水提法提取淀粉,观察淀粉颗粒形态,测定其组成、粒径、理化性质及体外抗消化特性。结果表明,金冠豆角籽粒淀粉中直链淀粉和支链淀粉的含量分别为47.39%和52.07%,淀粉的颗粒形态与豌豆淀粉类似,呈卵圆形或球型,颗粒表面光滑完整,粒径为75.10μm。金冠豆角籽粒淀粉的峰值粘度(212.20 RVU)、衰减值(68.50 RVU)较低,但回生值(96.80 RVU)高于玉米淀粉;金冠豆角籽粒淀粉糊化的起始温度(T_(0))、峰值温度(T_(p))显著高于豌豆淀粉(P<0.05)。金冠豆角籽粒淀粉的凝沉值在90 min时接近80%,凝沉速度显著高于玉米淀粉,透光率介于豌豆淀粉和玉米淀粉之间,持水性(WHC)和持油性(OHC)值分别为1.08和1.00 g/g,凝胶硬度小,属于易凝沉淀粉,其抗性淀粉(RS)含量为68.43%,高于玉米淀粉和豌豆淀粉(P<0.05),具有良好的抗消化特性。