14 yellow-seeded rapeseed lines (Brassia napus L.) from different genetic sources were used to analyze diversity of testa pigments content, oil and protein content, and RAPD markers. The results showed that the anthoc...14 yellow-seeded rapeseed lines (Brassia napus L.) from different genetic sources were used to analyze diversity of testa pigments content, oil and protein content, and RAPD markers. The results showed that the anthocyanin and melanin were the most important pigments in testa and their content were responsible for the variation in seed color ranging from orange to black yellow, 14 yellow-seeded lines could be classified into 3 groups: high anthocyanin content group with anthocyanin content over 2. 54 mg g-1 DW, the seed color was light yellow or orange; low pigments content group with low content of anthocyanin and melanin, the testa was transparent and the seed color was light yellow, greenish yellow or twany; high melanin content group with melanin content over 178. 4U(A290nm) , the testa was black, the seed color was black yellow. Oil content changed from 36.2% to 45. 5%, protein content from 21.1% to 27.7% , and the correlation analysis revealed that the oil content is highly significantly negatively correlated with the protein content. The cluster analysis showed that the extensive genetic variation existed among 14 yellow-seeded lines by using unweighted paired group method with arithmetic average (UPGMA) based on RAPD markers which were amplified with decamer primers, the genetic similarity among them ranged from 0. 25 to 0.909, and 14 yellow-seeded lines could put into 2 clusters corresponding to genome difference.展开更多
Brassica napus is an important cash crop broadly grown for the vegetable and oil values.Yellow-seeded B.napus is preferred by breeders due to its improved oil and protein quality,less pigments and lignin compared with...Brassica napus is an important cash crop broadly grown for the vegetable and oil values.Yellow-seeded B.napus is preferred by breeders due to its improved oil and protein quality,less pigments and lignin compared with the blackseeded counterpart.This study compared the differences in flavonoid and fatty acid contents between yellow rapeseed from the progenies of B.napus-Sinapis alba somatic hybrids and the black-seeded counterpart using RNA-seq analysis.Through HPLC-PDA-ESI(-)/MSanalysis,it was found that phenylpropanoids and flavonoids(i.e.,isorhamnetin,epicatechin,kaempferol,and other derivatives)in yellow seed were significantly lower than those in black seed.The fatty acid(FA)content in yellow rapeseed was higher than that in black rapeseed due to the variation of C16:0,C18:0,C18:1,C18:2,and C18:3 contents.RNA-seq analysis of seeds at four and five weeks after flowering(WAF)indicated that differentially expressed genes(DEGs)between black and yellow rapeseeds were enriched in flavonoid and FA biosynthesis,including BnTT3,BnTT4,BnTT18,and BnFAD2.Also,genes related to FA biosynthesis,desaturation and elongation(FAD3,LEC1,FUS3,and LPAT2)in yellow seed were up-regulated compared to those in black seed,while genes involved in beta-oxidation cycle(AIM1 and KAT2)of yellow seed were down-regulated compared to those in black seed.The DEGs related to the variation of flavonoids,phenylpropanoids,and FAs would help improve the knowledge of yellow seed character in B.napus and promote rapeseed improvement.展开更多
以甘蓝型黄籽油菜冷榨浸出粕为材料,测定了蛋白质、粗纤维、木质素、可溶性糖和多酚含量,并对黄籽油菜制备浓缩蛋白条件进行了初步探索。结果表明,未脱壳的黄籽油菜饼粕具有蛋白质含量高、抗营养物质含量低的特点,采用乙醇脱糖即可制备...以甘蓝型黄籽油菜冷榨浸出粕为材料,测定了蛋白质、粗纤维、木质素、可溶性糖和多酚含量,并对黄籽油菜制备浓缩蛋白条件进行了初步探索。结果表明,未脱壳的黄籽油菜饼粕具有蛋白质含量高、抗营养物质含量低的特点,采用乙醇脱糖即可制备浓缩蛋白。黄籽浸提粕的蛋白质含量达50.31%,可溶性糖含量为5.90%,粗纤维和木质素分别为4.40%和1.05%;黑籽粕蛋白质含量为38.38%,可溶性糖含量为5.43%,粗纤维和木质素分别为10%和6.10%。采用多因素正交试验对乙醇浸提工艺条件进行优化,方差分析表明乙醇浓度和浸提温度对蛋白质含量的影响分别达显著和极显著水平。在乙醇浓度95%、浸提温度70℃、料液比1∶7、浸提5次、每次20 m in的条件下,获得的甘蓝型黄籽浓缩蛋白产品蛋白含量达60.07%,赖氨酸等必需氨基酸含量显著提高,氨基酸总量达49.51%,各氨基酸比例均匀,硫甙脱除率达74%,并且浓缩蛋白的溶解度、氮溶解性、吸水性、吸油性、乳化性、起泡性与泡沫稳定性等功能特性良好。展开更多
文摘14 yellow-seeded rapeseed lines (Brassia napus L.) from different genetic sources were used to analyze diversity of testa pigments content, oil and protein content, and RAPD markers. The results showed that the anthocyanin and melanin were the most important pigments in testa and their content were responsible for the variation in seed color ranging from orange to black yellow, 14 yellow-seeded lines could be classified into 3 groups: high anthocyanin content group with anthocyanin content over 2. 54 mg g-1 DW, the seed color was light yellow or orange; low pigments content group with low content of anthocyanin and melanin, the testa was transparent and the seed color was light yellow, greenish yellow or twany; high melanin content group with melanin content over 178. 4U(A290nm) , the testa was black, the seed color was black yellow. Oil content changed from 36.2% to 45. 5%, protein content from 21.1% to 27.7% , and the correlation analysis revealed that the oil content is highly significantly negatively correlated with the protein content. The cluster analysis showed that the extensive genetic variation existed among 14 yellow-seeded lines by using unweighted paired group method with arithmetic average (UPGMA) based on RAPD markers which were amplified with decamer primers, the genetic similarity among them ranged from 0. 25 to 0.909, and 14 yellow-seeded lines could put into 2 clusters corresponding to genome difference.
基金funded by the National Natural Science Foundation of China(U20A2028 and 31972963)the Open Funds of the Key Laboratory of Plant Functional Genomics of the Ministry of Education,China(ML201804)+2 种基金the Project of Special Funding for Crop Science Discipline Development,China(yzuxk202006)the Priority Academic Program Development of Jiangsu Higher Education Institutions,Chinathe Yangzhou University for Excellent Talent Support Program,China。
文摘Brassica napus is an important cash crop broadly grown for the vegetable and oil values.Yellow-seeded B.napus is preferred by breeders due to its improved oil and protein quality,less pigments and lignin compared with the blackseeded counterpart.This study compared the differences in flavonoid and fatty acid contents between yellow rapeseed from the progenies of B.napus-Sinapis alba somatic hybrids and the black-seeded counterpart using RNA-seq analysis.Through HPLC-PDA-ESI(-)/MSanalysis,it was found that phenylpropanoids and flavonoids(i.e.,isorhamnetin,epicatechin,kaempferol,and other derivatives)in yellow seed were significantly lower than those in black seed.The fatty acid(FA)content in yellow rapeseed was higher than that in black rapeseed due to the variation of C16:0,C18:0,C18:1,C18:2,and C18:3 contents.RNA-seq analysis of seeds at four and five weeks after flowering(WAF)indicated that differentially expressed genes(DEGs)between black and yellow rapeseeds were enriched in flavonoid and FA biosynthesis,including BnTT3,BnTT4,BnTT18,and BnFAD2.Also,genes related to FA biosynthesis,desaturation and elongation(FAD3,LEC1,FUS3,and LPAT2)in yellow seed were up-regulated compared to those in black seed,while genes involved in beta-oxidation cycle(AIM1 and KAT2)of yellow seed were down-regulated compared to those in black seed.The DEGs related to the variation of flavonoids,phenylpropanoids,and FAs would help improve the knowledge of yellow seed character in B.napus and promote rapeseed improvement.
文摘以甘蓝型黄籽油菜冷榨浸出粕为材料,测定了蛋白质、粗纤维、木质素、可溶性糖和多酚含量,并对黄籽油菜制备浓缩蛋白条件进行了初步探索。结果表明,未脱壳的黄籽油菜饼粕具有蛋白质含量高、抗营养物质含量低的特点,采用乙醇脱糖即可制备浓缩蛋白。黄籽浸提粕的蛋白质含量达50.31%,可溶性糖含量为5.90%,粗纤维和木质素分别为4.40%和1.05%;黑籽粕蛋白质含量为38.38%,可溶性糖含量为5.43%,粗纤维和木质素分别为10%和6.10%。采用多因素正交试验对乙醇浸提工艺条件进行优化,方差分析表明乙醇浓度和浸提温度对蛋白质含量的影响分别达显著和极显著水平。在乙醇浓度95%、浸提温度70℃、料液比1∶7、浸提5次、每次20 m in的条件下,获得的甘蓝型黄籽浓缩蛋白产品蛋白含量达60.07%,赖氨酸等必需氨基酸含量显著提高,氨基酸总量达49.51%,各氨基酸比例均匀,硫甙脱除率达74%,并且浓缩蛋白的溶解度、氮溶解性、吸水性、吸油性、乳化性、起泡性与泡沫稳定性等功能特性良好。
