[ Objective] This study aimed to investigate the biological effects of laser-induced mutation on fibrous roots of yellow skin onion. [ Method] Wet seeds of two yellow skin onion cultivars were irradiated by CO2 laser ...[ Objective] This study aimed to investigate the biological effects of laser-induced mutation on fibrous roots of yellow skin onion. [ Method] Wet seeds of two yellow skin onion cultivars were irradiated by CO2 laser and He-Ne laser at three dosage levels separately. A randomized complete block design with three replications was adopted. The biological effects of laser-induced mutation on fibrous roots of Ll-generation yellow skin onion were investigated with biostatistics and physiological and biochemical methods. [Result] Significant variations in the biological effects caused by various laser treatments were observed in the length, quantity, fresh weight and activity of onion fibrous roots. Specifically, the variation in fibrous root length induced by different types of laser reached 5% significance level; significant variation was observed in fibrous roots of different onion cultivars induced by laser, while the variation among each treatment did not reach 5% sig- nifieance level ; the variation in fibrous root quantity induced by different dosage levels of laser reached 5% significance level ; laser radiation showed stimulating effect on root activity of onion. [ Conclusion] This study provided reference for laser-induced breeding of yellow skin onion.展开更多
本试验旨在研究小麦-豆粕型饲粮中添加不同水平金菊黄和一品红对黄羽肉鸡生长性能,背部皮肤、腹部皮肤、胫脚和腹脂颜色以及色素沉积基因表达的影响,为天然类胡萝卜素在肉鸡上的应用提供参考。选取540只健康状况良好且体重相近的1日龄...本试验旨在研究小麦-豆粕型饲粮中添加不同水平金菊黄和一品红对黄羽肉鸡生长性能,背部皮肤、腹部皮肤、胫脚和腹脂颜色以及色素沉积基因表达的影响,为天然类胡萝卜素在肉鸡上的应用提供参考。选取540只健康状况良好且体重相近的1日龄麻黄肉公鸡,随机分为3组,每组6个重复,每个重复30只鸡。对照组饲喂基础饲粮,色素1组在基础饲粮中添加1000 mg/kg金菊黄和1000 mg/kg一品红,色素2组在基础饲粮中添加2000 mg/kg金菊黄和2000 mg/kg一品红。试验期48 d。结果表明:1)色素1组48日龄体重以及22~48日龄和1~48日龄平均日增重均显著高于对照组(P<0.05),色素1、2组22~48日龄和1~48日龄料重比显著低于对照组(P<0.05)。2)对于背部皮肤,与对照组相比,色素2组21日龄亮度(L^(∗))值显著降低(P<0.05),色素1、2组屠宰后45 min L^(∗)值显著降低(P<0.05);色素2组35日龄红度(a^(∗))值显著升高(P<0.05),色素1、2组屠宰后45 min和48 h a^(∗)值均显著升高(P<0.05);色素1、2组21、35、48日龄以及屠宰后45 min和48 h黄度(b^(∗))值均显著升高(P<0.05),且色素2组21、35和48日龄b^(∗)值显著高于色素1组(P<0.05)。3)对于腹部皮肤,与对照组相比,色素2组屠宰后45 min和48 h L^(∗)值显著降低(P<0.05);色素1、2组21日龄、48日龄以及屠宰后45 min和48 h a^(∗)值显著升高(P<0.05),且色素2组21日龄a^(∗)值显著高于色素1组(P<0.05);色素1、2组21、35、48日龄以及屠宰后45 min和48 h b^(∗)值均显著升高(P<0.05),且色素2组21日龄和屠宰后48 h b^(∗)值显著高于色素1组(P<0.05)。4)对于胫脚,与对照组相比,色素1、2组35日龄L^(∗)值显著降低(P<0.05);色素1、2组21、35和48日龄a^(∗)值显著升高(P<0.05);色素1、2组21、35和48日龄b^(∗)值显著升高(P<0.05),且色素2组35和48日龄b^(∗)值显著高于色素1组(P<0.05)。5)对于腹脂,与对照组相比,色素1、2组48日龄a^(∗)和b^(∗)值均显著升高(P<0.05),且色素2组48日龄b^(∗)值显著高于色素1组(P<0.05)。6)与对照组相比,色素1、2组皮肤、肝脏和肾脏中β-胡萝卜素-9′,10′-加氧酶(BCO2)基因表达量均显著升高(P<0.05)。综上所述,小麦-豆粕型饲粮中添加金菊黄和一品红可改善肉鸡生长性能以及不同阶段皮肤和腹脂着色效果,同时影响色素沉积基因表达,且低添加水平(1000 mg/kg)改善生长性能效果优于高添加水平(2000 mg/kg),但高添加水平着色效果优于低添加水平。展开更多
基金Supported by Natural Science Foundation of Sichuan Education Department(2008ZA033)
文摘[ Objective] This study aimed to investigate the biological effects of laser-induced mutation on fibrous roots of yellow skin onion. [ Method] Wet seeds of two yellow skin onion cultivars were irradiated by CO2 laser and He-Ne laser at three dosage levels separately. A randomized complete block design with three replications was adopted. The biological effects of laser-induced mutation on fibrous roots of Ll-generation yellow skin onion were investigated with biostatistics and physiological and biochemical methods. [Result] Significant variations in the biological effects caused by various laser treatments were observed in the length, quantity, fresh weight and activity of onion fibrous roots. Specifically, the variation in fibrous root length induced by different types of laser reached 5% significance level; significant variation was observed in fibrous roots of different onion cultivars induced by laser, while the variation among each treatment did not reach 5% sig- nifieance level ; the variation in fibrous root quantity induced by different dosage levels of laser reached 5% significance level ; laser radiation showed stimulating effect on root activity of onion. [ Conclusion] This study provided reference for laser-induced breeding of yellow skin onion.
文摘目的:探讨荆防方加减联合红黄光及重组牛碱性成纤维细胞生长因子(rb-bFGF)凝胶治疗面部糖皮质激素依赖性皮炎(Facial corticosteroid addictive dermatitis,FCAD)的效果。方法:根据治疗方法不同将2022年2月-2023年8月笔者医院的90例FCAD患者分为对照组和观察组,各45例。对照组使用红黄光照射治疗,观察组同时使用荆防方治疗。对比两组的临床疗效、症状评分、皮肤屏障功能、血清炎症因子水平、不良反应。结果:观察组的有效率(91.11%)高于对照组(73.33%)(P<0.05),观察组治疗后潮红、瘙痒、干燥脱屑、灼热疼痛的症状评分均低于对照组(P<0.05),观察组治疗后的皮脂含量、皮肤角质层含水量(Cuticle water conten,WCSC)高于对照组,经皮肤水分流失量(Transcutaneous water loss,TEWL)低于对照组(P<0.05),观察组治疗后的干扰素-γ(INF-γ)、免疫球蛋白-E(IgE)、白细胞介素-4(IL-4)水平均低于对照组(P<0.05),两组不良反应比较,差异无统计学意义(P>0.05)。结论:荆防方加减联合红黄光及rb-bFGF凝胶治疗FCAD的效果显著,能够减轻患者的症状,提高皮肤屏障功能,改善炎症状态。
文摘本试验旨在研究小麦-豆粕型饲粮中添加不同水平金菊黄和一品红对黄羽肉鸡生长性能,背部皮肤、腹部皮肤、胫脚和腹脂颜色以及色素沉积基因表达的影响,为天然类胡萝卜素在肉鸡上的应用提供参考。选取540只健康状况良好且体重相近的1日龄麻黄肉公鸡,随机分为3组,每组6个重复,每个重复30只鸡。对照组饲喂基础饲粮,色素1组在基础饲粮中添加1000 mg/kg金菊黄和1000 mg/kg一品红,色素2组在基础饲粮中添加2000 mg/kg金菊黄和2000 mg/kg一品红。试验期48 d。结果表明:1)色素1组48日龄体重以及22~48日龄和1~48日龄平均日增重均显著高于对照组(P<0.05),色素1、2组22~48日龄和1~48日龄料重比显著低于对照组(P<0.05)。2)对于背部皮肤,与对照组相比,色素2组21日龄亮度(L^(∗))值显著降低(P<0.05),色素1、2组屠宰后45 min L^(∗)值显著降低(P<0.05);色素2组35日龄红度(a^(∗))值显著升高(P<0.05),色素1、2组屠宰后45 min和48 h a^(∗)值均显著升高(P<0.05);色素1、2组21、35、48日龄以及屠宰后45 min和48 h黄度(b^(∗))值均显著升高(P<0.05),且色素2组21、35和48日龄b^(∗)值显著高于色素1组(P<0.05)。3)对于腹部皮肤,与对照组相比,色素2组屠宰后45 min和48 h L^(∗)值显著降低(P<0.05);色素1、2组21日龄、48日龄以及屠宰后45 min和48 h a^(∗)值显著升高(P<0.05),且色素2组21日龄a^(∗)值显著高于色素1组(P<0.05);色素1、2组21、35、48日龄以及屠宰后45 min和48 h b^(∗)值均显著升高(P<0.05),且色素2组21日龄和屠宰后48 h b^(∗)值显著高于色素1组(P<0.05)。4)对于胫脚,与对照组相比,色素1、2组35日龄L^(∗)值显著降低(P<0.05);色素1、2组21、35和48日龄a^(∗)值显著升高(P<0.05);色素1、2组21、35和48日龄b^(∗)值显著升高(P<0.05),且色素2组35和48日龄b^(∗)值显著高于色素1组(P<0.05)。5)对于腹脂,与对照组相比,色素1、2组48日龄a^(∗)和b^(∗)值均显著升高(P<0.05),且色素2组48日龄b^(∗)值显著高于色素1组(P<0.05)。6)与对照组相比,色素1、2组皮肤、肝脏和肾脏中β-胡萝卜素-9′,10′-加氧酶(BCO2)基因表达量均显著升高(P<0.05)。综上所述,小麦-豆粕型饲粮中添加金菊黄和一品红可改善肉鸡生长性能以及不同阶段皮肤和腹脂着色效果,同时影响色素沉积基因表达,且低添加水平(1000 mg/kg)改善生长性能效果优于高添加水平(2000 mg/kg),但高添加水平着色效果优于低添加水平。
