Leaf color mutants are ideal materials for studying many plant physiological and metabolic processes such as photosynthesis,photomorphogenesis,hormone physiology and disease resistance.In this study,the genetically st...Leaf color mutants are ideal materials for studying many plant physiological and metabolic processes such as photosynthesis,photomorphogenesis,hormone physiology and disease resistance.In this study,the genetically stable yellow-green leaf mutant ygl16 was identified from mutated“Xinong 1B”.Compared with the wild type,the pigment concentration and photosynthetic capacity of the ygl16 decreased significantly.The ultrastructural observation showed that the distribution of thylakoid lamellae was irregular in ygl16 chloroplasts,and the grana and matrix lamellae were blurred and loose in varied degrees,and the chloroplast structure was disordered,while the osmiophilic corpuscles increased.The results of the genetic analysis and mapping showed that the phenotype of ygl16 was controlled by a pair of recessive nuclear gene.The gene located in the 56Kb interval between RM25654 and R3 on the long arm of chromosome 10.The sequencing results showed that the 121st base of the first intron of the candidate gene OsPORB/FGL changed from A to T in the interval.qRT-PCR results showed that the expression of chlorophyll synthase-related genes in the mutant decreased.展开更多
Enhancing photosynthesis efficiency is considered as one of the most crucial targets during wheat breeding.However,the molecular basis underlying high photosynthesis efficiency is not well understood up to now.In this...Enhancing photosynthesis efficiency is considered as one of the most crucial targets during wheat breeding.However,the molecular basis underlying high photosynthesis efficiency is not well understood up to now.In this study,we investigated the protein expression profile of wheat Jimai5265yg mutant,which is a yellow-green mutant with chlorophylls b deficiency but high photosynthesis efficiency.Though TMT-labeling quantitative proteomics analysis,a total of 72 differential expressed proteins(DEPs)were obtained between the mutant and wild type(WT).GO analysis found that they significantly enriched in thylakoid membrane,pigment binding,magnesium chelatase activity and response to light intensity.KEGG analysis showed that they involved in photosynthesis-antenna protein as well as porphyrin and chlorophyll metabolism.Finally,118 RNA editing events were found between mutant and WT genotype.The A to C editing in the 3-UTR of TraesCS6D02G401500 lead to its high expression in mutant through removing the inhibition of tae-miR9781,which might have vital role in regulating the yellow-green mutant.This study provided some useful clues about the molecular basis of Jimai5265yg mutant as well as chlorophylls metabolism in wheat.展开更多
To clarify the influence of yellow,green,and proportional yellow-green light on the capture effect,western flower thrips,Frankliniella occidentalis(Pergande),were captured using different self-made light sources in a ...To clarify the influence of yellow,green,and proportional yellow-green light on the capture effect,western flower thrips,Frankliniella occidentalis(Pergande),were captured using different self-made light sources in a greenhouse.The bio-activity capture effect of thrips was regulated by light and analyzed to determine the reasons for the changes in their capture by light.The results showed that the thrips’capture effect induced by different light sources with the same brightness was positively correlated with night temperature.When the average night temperature was 27℃(19:00-21:30),the capture effect was optimal,indicating that the coupling effect of light temperature can regulate the capture effect of thrips.Green light intensified and yellow light inhibited the visual trend sensitivity of thrips to yellow-green light of differing proportions.The capture effect trapped by a green-yellow light ratio of 4:1 was optimal(1088.00 individuals in night time),while that of yellow light was the worst(456.67 individuals/night),thus,indicating that visual trend sensitivity of thrips to green light was higher than that of yellow light.Such differences originated from the differences in the photoelectric thermal conversion effect of spectral optical properties,and the spectral photo-thermal effect was the main reason that thrips produced a light-trapped behavior.Night light enhanced the sensitivity of thrips’responses to a white adhesive board during the daytime,and the effect of yellow light intensity was the strongest(1563.00 individuals in the daytime),while that of green light was the weakest(75.33 individuals in the day time).Additionally,yellow light intensified while green light inhibited the regulatory effect of different proportions of yellow-green light on the bio-activity of thrips.However,the capture effect of day and night corresponding to 4:1 green-yellow light was the best(2019.67 individuals in day and night).The function of the photo-thermal effect on the capture effect of thrips was affected by the decrease in night temperature,but the photo-electro-thermal effects of night light intensified the bio-activity of thrips in the daytime and enhanced their color sensitivity.The results provide a theoretical basis for the development of pest light induction equipment.展开更多
基金supported by grants from the Project of Creating High Quality,Disease Resistance and High Combining Ability CMS Lines(Grant No.cstc2018jscx-msybX0250)Chongqing Technology Innovation and Application Demonstration Project and the Project of High Photosynthetic Efficiency Rice Breeding Technology System(Grant No.2017YFD0100201)the National Key Research and Development Program“Seven Crops Breeding”.
文摘Leaf color mutants are ideal materials for studying many plant physiological and metabolic processes such as photosynthesis,photomorphogenesis,hormone physiology and disease resistance.In this study,the genetically stable yellow-green leaf mutant ygl16 was identified from mutated“Xinong 1B”.Compared with the wild type,the pigment concentration and photosynthetic capacity of the ygl16 decreased significantly.The ultrastructural observation showed that the distribution of thylakoid lamellae was irregular in ygl16 chloroplasts,and the grana and matrix lamellae were blurred and loose in varied degrees,and the chloroplast structure was disordered,while the osmiophilic corpuscles increased.The results of the genetic analysis and mapping showed that the phenotype of ygl16 was controlled by a pair of recessive nuclear gene.The gene located in the 56Kb interval between RM25654 and R3 on the long arm of chromosome 10.The sequencing results showed that the 121st base of the first intron of the candidate gene OsPORB/FGL changed from A to T in the interval.qRT-PCR results showed that the expression of chlorophyll synthase-related genes in the mutant decreased.
基金supported by the National Key Research and Development Plan[2017YFD0100706]National Natural Science Foundation of China[31871618].
文摘Enhancing photosynthesis efficiency is considered as one of the most crucial targets during wheat breeding.However,the molecular basis underlying high photosynthesis efficiency is not well understood up to now.In this study,we investigated the protein expression profile of wheat Jimai5265yg mutant,which is a yellow-green mutant with chlorophylls b deficiency but high photosynthesis efficiency.Though TMT-labeling quantitative proteomics analysis,a total of 72 differential expressed proteins(DEPs)were obtained between the mutant and wild type(WT).GO analysis found that they significantly enriched in thylakoid membrane,pigment binding,magnesium chelatase activity and response to light intensity.KEGG analysis showed that they involved in photosynthesis-antenna protein as well as porphyrin and chlorophyll metabolism.Finally,118 RNA editing events were found between mutant and WT genotype.The A to C editing in the 3-UTR of TraesCS6D02G401500 lead to its high expression in mutant through removing the inhibition of tae-miR9781,which might have vital role in regulating the yellow-green mutant.This study provided some useful clues about the molecular basis of Jimai5265yg mutant as well as chlorophylls metabolism in wheat.
基金supported by the Scientific and technological project in Henan Province,China(Grant No.212102110139,212102110229)the Special Project of Xinxiang Science and Technology,China(Grant No.21ZD003)the Agriculture Research System of MOF and MARA(Grant No.CARS-03).
文摘To clarify the influence of yellow,green,and proportional yellow-green light on the capture effect,western flower thrips,Frankliniella occidentalis(Pergande),were captured using different self-made light sources in a greenhouse.The bio-activity capture effect of thrips was regulated by light and analyzed to determine the reasons for the changes in their capture by light.The results showed that the thrips’capture effect induced by different light sources with the same brightness was positively correlated with night temperature.When the average night temperature was 27℃(19:00-21:30),the capture effect was optimal,indicating that the coupling effect of light temperature can regulate the capture effect of thrips.Green light intensified and yellow light inhibited the visual trend sensitivity of thrips to yellow-green light of differing proportions.The capture effect trapped by a green-yellow light ratio of 4:1 was optimal(1088.00 individuals in night time),while that of yellow light was the worst(456.67 individuals/night),thus,indicating that visual trend sensitivity of thrips to green light was higher than that of yellow light.Such differences originated from the differences in the photoelectric thermal conversion effect of spectral optical properties,and the spectral photo-thermal effect was the main reason that thrips produced a light-trapped behavior.Night light enhanced the sensitivity of thrips’responses to a white adhesive board during the daytime,and the effect of yellow light intensity was the strongest(1563.00 individuals in the daytime),while that of green light was the weakest(75.33 individuals in the day time).Additionally,yellow light intensified while green light inhibited the regulatory effect of different proportions of yellow-green light on the bio-activity of thrips.However,the capture effect of day and night corresponding to 4:1 green-yellow light was the best(2019.67 individuals in day and night).The function of the photo-thermal effect on the capture effect of thrips was affected by the decrease in night temperature,but the photo-electro-thermal effects of night light intensified the bio-activity of thrips in the daytime and enhanced their color sensitivity.The results provide a theoretical basis for the development of pest light induction equipment.
