UHPLC-MS/MS analysis of cAMP and cGMP in rat plasma as potential biomarkers of Yin-Yang disharmony in traditional Chinese medicine

Cyclic 3’,5’-adenosine monophosphate(cAMP)and cyclic 3’,5’-guanosine monophosphate(cGMP)are considered as potential biomarkers for Yin-Yang disharmony in traditional Chinese medicine.However,phosphodiesterase-mediated ex vivo degradation of these molecules in biological samples may result in their underestimation.In the present study,a ultra-high performance liquid chromatography-tandem mass spectrometry(UHPLC-MS/MS)method was developed for determination of cAMP and cGMP in rat plasma,with special consideration of their stability ex vivo.Following precipitation of proteins from plasma samples with 0.4 M perchloric acid,the analytes were chromatographed on a Shimadzu Shimpack-XR-ODS II column with 2.5 mM ammonium acetate and methanol in gradient mode.The MS/MS detection was performed using multiple reaction monitoring in the positive electrospray ionization mode.The lower limit of quantification was 0.27 ng/mL for cAMP and 0.37 ng/mL for cGMP.The method was used to determine the plasma cAMP and cGMP levels in normal and Yin deficiency diabetic rats treated with or without Rehmannia glutinosa.The developed method may be useful for evaluating the regulatory effects of Chinese herbal medicine on the levels of cAMP and cGMP in the body.

Clinical study on microscopic syndrome differentiation and traditional Chinese medicine treatment for liver stomach disharmony in chronic gastritis

BACKGROUND Chronic gastritis(CG)is a common gastrointestinal disorder characterized by inflammation of the stomach lining.Liver-stomach disharmony(LSD)syndrome is believed to contribute to CG symptoms.AIM To evaluate the efficacy and safety of microcosmic syndrome differentiation and Chinese herbal medicine(CHM)treatment in patients with CG and LSD syndrome.METHODS Sixty-four patients with CG and LSD syndrome were randomly divided into two groups:The treatment group received CHM based on microcosmic syndrome differentiation and the control group received conventional Western medicine.The treatment course lasted 12 wk.The primary outcome was improvement in dyspeptic symptoms,measured using the Nepean Dyspepsia Index.The secondary outcomes included the improvement rate of endoscopic findings,histopathological findings,and microcosmic syndrome scores and the incidence of adverse events.RESULTS After 12 wk of treatment,the treatment group showed significantly greater improvement in dyspeptic symptoms than the control group(93.75%vs 65.63%,P<0.01).The treatment group also showed a significantly higher improvement rate in endoscopic findings than the control group(81.25%vs 53.13%,P<0.05).The improvement rates of histopathological findings and microcosmic syndrome scores were not significantly different between the two groups(P>0.05).No serious adverse events were observed in either group.CONCLUSION Microcosmic syndrome differentiation and CHM treatment can effectively improve dyspeptic symptoms and endoscopic findings in patients with CG and LSD syndrome and have a good safety profile.Further studies with larger sample sizes and longer follow-up periods are required to confirm the long-term efficacy and mechanism of action of this treatment.

核转录因子Yin-Yang1与中医阴阳学说

Yin- Yang 1 ( YY1 )是一种具有双重转录活性的核转录因子 ,广泛存在于真核细胞中 ,自被发现十余年来 ,国外对其研究报道逐年增多 ,对其在基因转录调控、分子信号转导中所表现出的关键作用的认识日益引起重视。本文综述了近年来 YY1研究的部分相关文献 ,分析了 YY1与中医阴阳的关系 ,以期为中医阴阳学说的现代研究带来新的思路。

An Improved Dynamic Core for a Non-hydrostatic Model System on the Yin-Yang Grid

A 3D dynamic core of the non-hydrostatic model GRAPES（Global/Regional Assimilation and Prediction System） is developed on the Yin-Yang grid to address the polar problem and to enhance the computational efficiency. Three-dimensional Coriolis forcing is introduced to the new core, and full representation of the Coriolis forcing makes it straightforward to share code between the Yin and Yang subdomains. Similar to that in the original GRAPES model, a semi-implicit semi-Lagrangian scheme is adopted for temporal integration and advection with additional arrangement for cross-boundary transport. Under a non-centered second-order temporal and spatial discretization, the dry nonhydrostatic frame is summarized as the solution of an elliptical problem. The resulting Helmholtz equation is solved with the Generalized Conjugate Residual solver in cooperation with the classic Schwarz method. Even though the coefficients of the equation are quite different from those in the original model, the computational procedure of the new core is just the same. The bi-cubic Lagrangian interpolation serves to provide Dirichlet-type boundary conditions with data transfer between the subdomains. The dry core is evaluated with several benchmark test cases, and all the tests display reasonable numerical stability and computing performance. Persistency of the balanced flow and development of both the mountain-induced Rossby wave and Rossby–Haurwitz wave confirms the appropriate installation of the 3D Coriolis terms in the semi-implicit semi-Lagrangian dynamic core on the Yin-Yang grid.

Influence of TBX21 T-1993C variant on autoimmune hepatitis development by Yin-Yang 1 binding

AIM To investigated the mechanism of the association between the TBX21 T-1993 C promoter polymorphism and autoimmune hepatitis type 1(AIH-1) development.METHODS In vivo,In vivo,and reporter analyses were performed to determine the function of transcription factors binding to the T-1993 C element of the TBX21 promoter in human CD4+ T and B cell lines. Flow cytometry and quantitative real-time PCR were used to analyze T-box transcription factor(T-bet) and interferon-γ(IFN-γ) expressions in CD4+ T cells,B cells and monocytes from the peripheral blood of AIH-1 patients including 5-1993 TC and 15-1993 TT genotype carriers,and healthy controls including 10-1993 TC and 25-1993 TT genotype carriers. Furthermore,a range of biochemical indices was measured simultaneously in the blood of AIH-1 patients.RESULTS TBX21-1993 C allele created a strong Yin-Yang 1(YY1)-binding site and decreased transcriptional activity of TBX21 promoter in human CD4+ T and B cells.Higher levels of T-bet and IFN-γ were detected in the circulating CD4+ T cells and B cells of AIH-1 patients carrying the TBX21-1993 TT genotype compared with the patients carrying the-1993 TC genotype and controls with the-1993 TC genotype. T-bet expression levels of circulating T cells and B cells were positively correlated with AIH-1 disease activity. Knockdown of YY1 with si RNA caused increased expression of T-bet and IFN-γ in peripheral blood mononuclear cells in AIH-1 patients. CONCLUSION The repression of TBX21 expression by high-affinity binding of YY1 to the-1993 C allele may contribute to a decreased development of AIH-1 via suppression of type 1 immunity.

An Accurate Multimoment Constrained Finite Volume Transport Model on Yin-Yang Grids

A global transport model is proposed in which a multimoment constrained finite volume （MCV） scheme is applied to a Yin-Yang overset grid. The MCV scheme defines 16 degrees of freedom （DOFs） within each element to build a 2D cubic reconstruction polynomial. The time evolution equations for DOFs are derived from constraint conditions on moments of line-integrated averages （LIA）, point values （PV）, and values of first-order derivatives （DV）. The Yin-Yang grid eliminates polar singularities and results in a quasi-uniform mesh. A limiting projection is designed to remove nonphysical oscillations around discontinuities. Our model was tested against widely used benchmarks; the competitive results reveal that the model is accurate and promising for developing general circulation models.

Liver-stomach disharmony pattern:theoretical basis, identification and treatment

The pattern of disharmony between the liver and stomach is widely observed in patients with digestive diseases,such as gastroesophageal reflux disease,chronic gastritis,and functional dyspepsia,etc.In order to gain a full and systematic understanding of this pattern,we reviewed the functions and physiological characteristics of the liver and stomach and the liver-stomach relationship.In this review,we discuss the pathogenesis and identification of the pattern,as well as the commonly used classical formulas for its treatment,including Left Metal Pill (Zuojin Wan) and Bupleurum Liver-Soothing Powder (Chaihu Shugan San).Besides,we provide two examples of clinical modifications of these formulas in the treatment of chronic gastritis and gastroesophageal reflux disease in this article.It should be noted that in clinical practice,these formulas should be used flexibly,and modified in accordance to the specific condition of the patient.

Implementation of CMA-GFS 3D-Var System on the Yin-Yang Grid

The global three-dimensional variational(3D-Var)data assimilation is implemented on a new quasi-uniform overset(Yin-Yang)grid on sphere.As a quasi-uniform spherical grid,it covers the sphere by overlapping two perpendicularly oriented grid components which is nothing but low latitude region of the usual latitude-longitude grid.Based on this characteristic of the Yin-Yang grid,it enables us to implement the regional 3D-Var system efficiently and accurately on the Yin or Yang component grid,respectively.The global analysis could update directly from the regional analysis since they have the same configurations like the precondition of eigenvalue decomposition for vertical direction,recursive filtering for horizontal direction,minimization method and observation operator and so on.However,the balance equation and vector wind are needed to be paid more attention on the Yin grid analysis due to its coordinate transformation.How to spread the observation information near the boundary of Yin and Yang grid is a key to the 3D-Var analysis.Extending double the horizontal correlation length distance in the overset boundary of Yin and Yang grid has successfully solved the problem.The results show that the analysis on the Yin-Yang grid is reasonable and similar to the result on the latitude-longitude(LAT-LON)grid.This paper provides a promising strategy for the development of a 3D-Var global system for overset grids.

A Clinical Study of Integrated Traditional Chinese and Western Medicine in the Treatment of Liver-Stomach Disharmony Functional Dyspepsia

Objective:To explore the clinical effect of integrated traditional Chinese and Western medicine in the treatment of liver-stomach disharmony functional dyspepsia.Methods:Sixty patients with functional dyspepsia of liver-stomach disharmony type admitted to our hospital from January to August 2022 were selected as research subjects and randomly divided into two groups,a study group and a control group,with 30 cases in each group.The main observations were stomachache or pain over bilateral flanks,emotionally depressed,belching,fullness and discomfort over the abdomen and flanks,acid regurgitation,loss of appetite,frequent sighing,noisy epigastric,and the treatment effect.Results:According to the classification of symptom severity on the traditional Chinese medicine(TCM)symptom score table,statistics were made on the corresponding severity of the main symptoms and secondary symptoms of the two groups of patients,and the data of the two groups were compared by Wilcoxon test.The results showed that there was no significant difference in the distribution of TCM symptoms between the two groups;the study group’s total effective rate of pain relief(recovery+markedly effective+effective)was 96.67%,and Fisher’s χ^(2) test indicated a significant difference in the total effective rate of pain relief between the two groups(P=0.027<0.05).Conclusion:The use of integrated traditional Chinese and western medicine is clearly better than the simple application of western medicine.It is safe and has no side effects.It can be used as a treatment for patients with functional dyspepsia.

气机升降理论内涵及不寐病辨证分型探讨

气机升降理论是“汉派李氏脑病流派”辨治不寐病的核心理论思想,基于气机升降理论将不寐病分为升降太过和升降不及两大类,根据气机运行的部位、气的多少、气机升降出入的不同形式将不寐病分为营卫不和型、脏腑气机失调型、阴阳不交型三大类型;每一类型又有各自不同亚型,共有卫阳虚不敛营阴证、卫气盛夜不入营阴证、卫气入于阴而日不得出于阳证、肝胆气机疏泄失调证、心肾气机上下相济失调证、脾升胃降枢机失调证、阳不入阴证、阳盛阴虚证、阴不潜阳证九种亚型,并表现出各自独有的临床特征。基于气机升降理论的辨证分型法补充完善了现阶段不寐病辨证分型类别,为临床诊治不寐病开拓了新思路。

Women in Chinese Philosophy:Yin-Yang Theory in Feminism Constructing

The traditional way of thinking about women in ancient Chinese philosophy was grounded on the concepts of Yin and Yang.Yin and Yang are the basic principles of the being,the interrelation and interaction between which provide the original force for the emergence of all beings in the world.In the traditional family construction,men as Yang master the affairs outside the family,and women as Yin master the affairs inside the family(男主外,女主内),which means that women normally had no voice in the social affairs.Her field was limited inside the family.Furthermore,according to Confucian doctrine“husband guides wife”(夫为妻纲),husband is the decision maker of a family,as a king in a state.The prejudice against woman has persisted to the present.This paper will show that within the Confucian tradition,the ontological principles of Yin and Yang have been abused to a certain degree,and women became the sacrifice of the interpretation with this prejudice.The author will argue in this paper,that the Yin-yang principle itself is very flexible.It contains much richer possibilities of interpretation than that had been interpreted in the Confucian tradition.The author’s argument is that if we recognized that each person is a complete Yin-Yang system,we would understand that every human being is a complete and independent person with both virtues of Yin and Yang.Yin is not a substance which restricts women,neither Yang to men.From this perspective,every woman has the potentiality to reach her universality and individuality.The author’s claim is that in building feminism with the resource of ancient Chinese thought,the concepts of Yin and Yang can be revived and given a new life if we think about them in a functional way.In this framework,Yin and Yang are correlative functions in social and family operation.The properties of Yin and Yang are determined only in each particular affair.People practicing functions are Yin or Yang in temporality.

Clinical effect of Yin-Yang blending acupuncture combined with Banxia Baizhu Tianma Decoction in treatment of cervical vertigo

Objective:To explore the clinical effect of Yin-yang blending acupuncture with traditional Chinese medicine combined with Banxia Baizhu Tianma Decoction in treating patients with cervical vertigo.Methods:120 patients with cervical vertigo treated in our hospital were selected,and the acupuncture group and the acupuncture group were divided into 60 cases each according to the order of the patients.The two groups of patients were given basic treatment measures.Xia Baizhu Tianma Decoction was treated,and the control group was given Yin and Yang blending acupuncture.The left and right vertebral arteries(LVA),right vertebral arteries(RVA),and basal arteries(BA)were compared before and after treatment in the two groups.,Clinical symptom score,clinical efficacy.Results:after treatment,the Vm values of LVA,RVA,and BA in the acupuncture group were lower than those in the acupuncture group(P<0.05);after treatment,acupuncture The dizziness,neck and shoulder pain,headache,daily life work,psychological and social adaptability scores of the drug group were higher than those of the acupuncture group(P<0.05);after treatment,the cure rate of the acupuncture group was 42.37%,and the effective rate was 37.29%.The efficiency was 16.95%,the inefficiency was 3.39%,the cure rate in the acupuncture group was 26.32%,the marked efficiency was 35.09%,the effective rate was 29.82%,and the ineffective rate was 8.77%.The clinical efficacy of the acupuncture group was better than that of the acupuncture group(P<0.05).Conclusion:Traditional Chinese medicine Yin-yang reconciliation acupuncture combined with Pinellia Baizhu Tianma Decoction is definitely effective in treating patients with cervical vertigo,and it can effectively improve the clinical symptoms and clinical effects of patients.

Disharmonious Ventricular Relationship and Topology for the Given Atrioventricular Connections.Contemporary Diagnostic Approach Using 3D Modeling and Printing

In the last issue,two case reports separately present examples of the extremely rare and complex congenital heart diseases that show concordant atrioventricular connections to the L-looped ventricles in the presence of situs solitus.Both cases highlight that the relationship between the two ventricles within the ventricular mass is not always harmonious with the given atrioventricular connection.Such disharmony between the connections and relationships requires careful assessment of the three basic facets of cardiac building blocks,namely their morphology,the relationship of their component parts,and their connections with the adjacent segments.3D imaging and printing can now facilitate an otherwise difficult diagnosis in such complex situations.Rotation of either the 3D images or the models permit accurate assessment of the ventricular topologic pattern by creating the right ventricular en-face septal view,thus facilitating placement of the observer’s hands.As we now emphasize,an alternative approach,which might prove more attractive to imagers,is to rotate the ventricular mass to provide the ventricular apical view,thus permitting determination of the ventricular relationship without using the hands.

The Dao of Mathematical Creativity,Symmetry Theories,and Yin-Yang Philosophy With Their Classroom Applications

This paper introduces creativity,one of the significant features in maths.The creative mathematical beauty of symmetry and asymmetry are worth investigating in the practicing of Taichi.Symmetrical variances make the real world.Symmetry can be regarded as a kind of mirroring,like the line symmetry,the mirror image of the other parts,or rotational symmetry.Such characteristics can be vividly demonstrated in the traditional Chinese philosophical yin-yang theory(YY).The accompanying of male and female reveals the friendly cooperative relationship.YY reflects the counterparts of vacancy and firm,inhale and exhale,noun and verb,theory and practice,light and heavy,slow and fast,the pace,the sound effect in the classroom.Such an analysis is undergone about yin and yang in Yi School and Dao De Ching(DDC).Furthermore,there are detailed arguments of the symmetric nature of every verse of DDC,revealing the cultural value of the language.Their applications in the class admit the recognition under the new liberal arts project by China’s Ministry of Education.Yet,the integration of research and teaching is required to go further.

The Chinese Yin-Yang Thinking in Antoni Tàpies’Artistic Creation

The writings and artistic works of Antoni Tàpies(2009;2011)mirror the influences of the cosmic vision of the Chinese yin-yang.Signs,numbers,and creative procedures related to this correlative thought prevail in his art.Tàpies integrated different iconological codes from the ancient Chinese thinking into his art to question the existential meaning of the universe and to express his thoughts on the cultural and socio-political controversies of his time.In other words,the images,media,and techniques that he utilized for his works were extensions of his life experiences intertwined with various socio-historical facts and his personal feelings.

Clinical observation on efficacy of compound of warming yang, descending turbidity and dredging collaterals in the treatment of diabetic kidney disease with Yin-Yang deficiency and blood stasis syndrome

Objective:To observe the clinical efficacy of compound of owarming yang,descending turbidity and dredging collaterals in the treatment of diabetic kidney disease with yin-yang deficiency and blood stasis syndrome.Methods:Seventy-six patients of diabetic kidney disease with yin-yang deficiency and blood stasis syndrome were randomly divided into observation group and control group,thirty-eight cases in each group.The control group was given conventional western medicine treatment,while the observation group took compound of owarming yang,descending turbidity and dredging collaterals orally on the basis of conventional western medicine treatment.The course of treatment covered for one month.Before and after treatment,we observed the scores of traditional Chinese medicine symptoms,indicators of renal function[serum creatinine(Scr),blood urea nitrogen(BUN),microalbuminuria(MALB)],indicators of glucose metabolism[fasting plasma glucose(FPG),2-hour postprandial blood glucose(2hPG),glycosylated hemoglobin(HbAlc)],indicators of hemorheology[plasma viscosity(PV),platelet aggregation rate(PAR),fibrinogen(FIB)],Cystatin-C(Cys-C),C-reactive protein(CRP)in the two groups.Results:After treatment,the clinical effect of the observation group was significantly better than the control group(P<0.05).The scores of traditional Chinese medicine symptoms,indicators of renal function(Scr、BUN、UAER),indicators of glucose metabolism(FPG、2hPG、HbAlc),indicators of hemorheology(PV、PAR、FIB),Cys-C and CRP in the two groups were decreased significantly compared with those before treatment(P<0.05),and the decrease in the observation group was superior to that in the control group(P<0.05).Conclusion:Compound of warming yang,descending turbidity and dredging collaterals has remarkable efficacy in treating of diabetic kidney disease patients with yin-yang deficiency and blood stasis syndrome by alleviating clinical symptoms,glucose metabolism,renal function and microcirculatory disturbance,and the mechanism related to alleviation of microinflammation.

Gender Relations in Kjell Askildsen’s A Lovely Place From the Perspective of Chinese Yin-Yang Theory

A Lovely Place(2019)is a collection of the Norwegian writer Kjell Askildsen’s short stories that is translated from German into Chinese by Yang Zhizi.It contains 18 short stories,whose male characters are mainly analyzed by researchers.The paper argues that female characters are playing an indispensable role in compensating the males’life stories and enlightening their worldviews according to Chinese Yin-Yang theory.The females can be classified into the following three groups in terms of their important relations to the males.Firstly,the female is the male’s companion in a certain part of the latter’s life.Secondly,the female facilitates the male’s intellectual growth either spontaneously or purposefully.Thirdly,the female struggles for independence to set an example for the male.The female characters in A Lovely Place present such femininity as beauty,energy,nostalgia,sentimentality,motherhood,etc.,stimulate the males to reflect on their own identities and fulfill their muscularity,and function as the genuine protagonists in the collection as far as the gender relations are concerned.

Piercing the Veil of the Disharmony of Edna's Marriage and Family

The paper aims mainly at deploring the reasons of the disharmony in marriage and family life of Edna, the heroine of Kate Chopin's The Awakening by analyzing the traditional social labor division and procreation concept. And it is the unbalanced social labor division and the patriarchal procreation conception that caused the alienation between the husband and wife rather than the capriciousness of female.

Pattern of disharmony between the heart and kidney: Theoretical basis, identification and treatment

'Harmony between the heart and kidney' refers to the physiological relationship between these two zang organs in Chinese medicine,while 'disharmony between the heart and kidney' (also called disharmony between fire and water) refers to the pathological state.The pattern of disharmony between the heart and kidney is widely observed in patients with insomnia,anxiety disorder and menopausal syndrome,etc..In order to gain a full and systematical understanding of this pattern,from the perspective of ancient Chinese philosophy and zang-fu theory in Chinese medicine,we systematically reviewed and discussed the functions and physiological characteristics of the heart and kidney,the origin and development of theories relating to heart-kidney relationship,the pathogenesis and identification of the pattern,as well as the commonly used classical formulas for its treatment,including Coptis and DonkeyHide Gelatin Decoction (Huánglián (E)jiāo Tāng) and Grand Communication Pill (Jiāotài Wán).Two examples of clinical modifications of these formulas in the treatment of insomnia and menopausal syndrome are provided in this article.It should be noted that in clinical practice,these formulas should be used flexibly,and modified in accordance to the condition of the patient.

左枳六君汤治疗肝胃不和型幽门螺杆菌阳性慢性胃炎的效果

目的探讨左枳六君汤治疗肝胃不和型幽门螺杆菌(Hp)阳性慢性胃炎的效果。方法选取2021年1月至2022年12月江西中医药高等专科学校附属医院(抚州市中医医院)收治的70例慢性胃炎患者(肝胃不和型Hp阳性)作为研究对象,按照随机数字表法分为观察组(35例)与对照组(35例),观察组采用常规西药四联治疗+左枳六君汤治疗方法,对照组采用常规西药四联治疗方法。比较两组的临床疗效、中医证候积分、Hp根除及复发率、血清生化指标。结果观察组的治疗总有效率(97.15%)、Hp根除率(94.28%)均高于对照组(82.85%、77.14%),Hp复发率(2.86%)低于对照组(17.14%),差异有统计学意义(P<0.05)。治疗后,观察组的中医证候积分低于对照组,血清胃泌素、胃动素水平均高于对照组,炎症因子(C反应蛋白、白介素-6、肿瘤坏死因子-α)水平低于对照组,差异有统计学意义(P<0.05)。结论在基础对症治疗上,将左枳六君汤引入肝胃不和型Hp阳性慢性胃炎患者的治疗中,能够提升临床综合治疗效果,作用显著,推荐参考使用。