Objective:To explore the intervention effects and mechanism of Simiao Yongan(SMYA)Decoction on carotid atherosclerotic plaque in ApoE knockout mice by antagonizing lipid metabolism of oxidation low density lipoprotein...Objective:To explore the intervention effects and mechanism of Simiao Yongan(SMYA)Decoction on carotid atherosclerotic plaque in ApoE knockout mice by antagonizing lipid metabolism of oxidation low density lipoprotein(Ox-LDL).Methods:ApoE-/-mice were fed high-fat feeding for 2 weeks,combined with perivascular collar placement(PCCP)and high fat feeding for 8 weeks to establish carotid AS plaque model.All the mouses were divided into 5 groups,after successful AS model preparation.The sham operation as well as model group intragastrically administered with deionized water.The rest mouses were intragastrically administered with SMYA decoction or Pioglitazone,or treated with Atorvastatin for 8 weeks.Then adopting automatic biochemical analyzer to test total cholesterol(TC),triglyceride(TG),high density lipoprotein cholesterol(HDL-C)and low density lipoprotein cholesterol(LDL-C)content.The content of ox-LDL in serum,which was tested by enzyme-linked immunosorbent assay(ELISA).The pathological changes of carotid artery were observed by hematoxylin-eosin(HE)staining and the vascular parameters were measured.Immunohistochemical method and Western blot assay was used to detect the manifestations of protein include fatty acid binding protein 4(FABP4)and peroxisome proliferator-activated receptorγ(PPARγ)as well as matrix metalloproteinase 2(MMP 2).Results:Compared with the sham operation group,the serum ox-LDL content of the model group was increased,and the intima thickness(IT),intima-media thickness(IT/MT),plaque area(PA)as well as lumen area ratio of plaque vessels(PA/LA)were enlarged(P<0.05).Immunohistochemistry and Western blot results showed that the relative expression of FABP4 and MMP2 in the model group increased markedly,while the relative expression of PPARγsignificantly decreased.Compared SMYA decoction group and Atorvastatin group as well as Pioglitazone with model group,the serum ox-LDL content was decreased(P<0.05).In the mean time,the IT,PA,IT/MT and PA/LA in carotid artery were reduced(P<0.05).Immunohistochemistry and Western blot results showed that the expression of FABP4 and MMP2 was reduced,while PPARγprotein expression increased of mouses in each administration group.Conclusion:SMYA decoction is able to antagonize Ox-LDL,increase the expression of PPARγ,decrease the level of FABP4,reduce MMP2 in carotid arteries of ApoE-/-atherosclerotic mice,and increase the stability of plaque.展开更多
对采自福建永安市下白垩统坂头组的似纵属Elatides雌球果化石进行了宏观形态和角质层特征的研究,这些球果均单独保存,较小,果鳞较多,螺旋状紧密排列,顶端收缩呈尖刺状,与Elatides的特征相符。根据球果的形态、果鳞及其球果中轴的特征,...对采自福建永安市下白垩统坂头组的似纵属Elatides雌球果化石进行了宏观形态和角质层特征的研究,这些球果均单独保存,较小,果鳞较多,螺旋状紧密排列,顶端收缩呈尖刺状,与Elatides的特征相符。根据球果的形态、果鳞及其球果中轴的特征,将这些球果鉴定为3个种,即永安似纵(新种)Elatides yonganensis Dai et Sun,sp.nov.、弯叶似纵(相似种)Elatidescf.curvifolius及一未定种Elatides sp.。其中重点描述了新种E.yonganensis的形态特征,该球果以中轴呈波状,果鳞顶端较长、较尖,腹面具有锯齿状的膜质小舌,每一果鳞具有大概5枚种子而区别于以前报道的任一已知种。同时对中国的Elatides化石记录进行了收集整理,Elatides属的球果化石在福建永安坂头组的发现表明其分布区可达25°N,丰富了该属植物的化石记录并扩大了其分布范围。展开更多
基金National Natural Science Foundation of China General Program(No.81874446)National Scicnce and Technology Major Project(No.2017ZX09304019)。
文摘Objective:To explore the intervention effects and mechanism of Simiao Yongan(SMYA)Decoction on carotid atherosclerotic plaque in ApoE knockout mice by antagonizing lipid metabolism of oxidation low density lipoprotein(Ox-LDL).Methods:ApoE-/-mice were fed high-fat feeding for 2 weeks,combined with perivascular collar placement(PCCP)and high fat feeding for 8 weeks to establish carotid AS plaque model.All the mouses were divided into 5 groups,after successful AS model preparation.The sham operation as well as model group intragastrically administered with deionized water.The rest mouses were intragastrically administered with SMYA decoction or Pioglitazone,or treated with Atorvastatin for 8 weeks.Then adopting automatic biochemical analyzer to test total cholesterol(TC),triglyceride(TG),high density lipoprotein cholesterol(HDL-C)and low density lipoprotein cholesterol(LDL-C)content.The content of ox-LDL in serum,which was tested by enzyme-linked immunosorbent assay(ELISA).The pathological changes of carotid artery were observed by hematoxylin-eosin(HE)staining and the vascular parameters were measured.Immunohistochemical method and Western blot assay was used to detect the manifestations of protein include fatty acid binding protein 4(FABP4)and peroxisome proliferator-activated receptorγ(PPARγ)as well as matrix metalloproteinase 2(MMP 2).Results:Compared with the sham operation group,the serum ox-LDL content of the model group was increased,and the intima thickness(IT),intima-media thickness(IT/MT),plaque area(PA)as well as lumen area ratio of plaque vessels(PA/LA)were enlarged(P<0.05).Immunohistochemistry and Western blot results showed that the relative expression of FABP4 and MMP2 in the model group increased markedly,while the relative expression of PPARγsignificantly decreased.Compared SMYA decoction group and Atorvastatin group as well as Pioglitazone with model group,the serum ox-LDL content was decreased(P<0.05).In the mean time,the IT,PA,IT/MT and PA/LA in carotid artery were reduced(P<0.05).Immunohistochemistry and Western blot results showed that the expression of FABP4 and MMP2 was reduced,while PPARγprotein expression increased of mouses in each administration group.Conclusion:SMYA decoction is able to antagonize Ox-LDL,increase the expression of PPARγ,decrease the level of FABP4,reduce MMP2 in carotid arteries of ApoE-/-atherosclerotic mice,and increase the stability of plaque.
文摘对采自福建永安市下白垩统坂头组的似纵属Elatides雌球果化石进行了宏观形态和角质层特征的研究,这些球果均单独保存,较小,果鳞较多,螺旋状紧密排列,顶端收缩呈尖刺状,与Elatides的特征相符。根据球果的形态、果鳞及其球果中轴的特征,将这些球果鉴定为3个种,即永安似纵(新种)Elatides yonganensis Dai et Sun,sp.nov.、弯叶似纵(相似种)Elatidescf.curvifolius及一未定种Elatides sp.。其中重点描述了新种E.yonganensis的形态特征,该球果以中轴呈波状,果鳞顶端较长、较尖,腹面具有锯齿状的膜质小舌,每一果鳞具有大概5枚种子而区别于以前报道的任一已知种。同时对中国的Elatides化石记录进行了收集整理,Elatides属的球果化石在福建永安坂头组的发现表明其分布区可达25°N,丰富了该属植物的化石记录并扩大了其分布范围。
