Identifying SNPs associated with birth weight and days to 100 kg traits in Yorkshire pigs based on genotyping-by-sequencing

Birth weight(BW)and days to 100 kg(D100)are important economic traits that are both affected by polygenes.However,the genetic architecture of these quantitative traits is still elusive.Genotyping-by-sequencing(GBS)data containing a large number of single nucleotide polymorphisms(SNPs)have become a powerful tool in genomic analysis.To better understand their complex genetic structure,a total of 600 Yorkshire pigs were sequenced using GBS technology.After quality control,279787 SNPs were generated for subsequent genome-wide association study(GWAS).A total of 30 genome-wide SNPs(P<1.79 E–07)were identified for D100.Furthermore,a total of 22 and 2 suggestive SNPs(P<3.57 E–06)were detected for D100 and BW,respectively.Of these,one locus located on SSC12(position:46226512 bp)were evaluated to affect both BW and D100 in Yorkshire pigs,indicating the pleiotropism in different traits.Considering the function of candidate genes,two genes,NSRP1 and DOCK7,were suggested as the most promising candidate genes involved in growth traits.Thus,use of GBS is able to identify novel variants and potential candidate genes for BW and D100,and provide an opportunity for improving pig growth traits using genomic selection in pigs.

Estimation of the Genetic Parameters on Reproduction Performances of the China Yorkshire Line SⅡ_1

[Objective] This study was to investigate the genetic parameters on reproduction performances of China Yorkshire line SII,. [Method] Using China Yorkshire line SII, as experimental animal, its genetic parameters on reproduction performance, such as total number of born （TNB）, the alive number of born （ANB）, the litter weight of born （LWB） and the litter weight at age of 21 days （/W21 ） , were estimated by using the DFREML method of multi-traits animal model. [Result] The heritability estimates of TNB, ANB, LWB and LW21 were 0.11,0.19, 0.14 and 0.05, respectively; and the genetic correlations between the traits as TNB/ANB, TNB/LWB, TNB/LW21, ANB/LWB, ANB/LW21, LWB/LW21 were 0.91, 0.76, 0.65, 0.85, 0.59 and 0.38, respectively. [ Conclusion] Main genetic parameters of reproduction traits in China Yorkshire line all, have all reached or even exceeded the requirements of breeding.

Comparison of Muscle Fiber Growth from F1Generations of Large Yorkshire Pig andErhualian Pig by Reciprocal Cross

[ Objective] To compare the muscle fiber growth between F1 generations of large Yorkshire pig and Erhualian pig by reciprocal cross, and provide a theoretical basis for the integrated breeding of meat quality traits and growth performances by rational utilization of hybrid combination. [ Method] The hybrid pigs of large Yorkshire pig and Erhualian pig were fed and managed in the same conditions. The reciprocal combinations were Erhualian pig ♀ × Yorkshire pigd, and Yorkshire pig ♀ × Erhualian pig♂ , respectively. At the age of 20, 70, 120 and 180 d, the Iongissimus dorsi muscle and thigh muscle were collected and made into frozen sections for hematoxylin and eosin （HE） staining, and then the muscle fiber area was determined. [ Result] The muscle fiber was thickened gradually with increasing age; and the absolute growth curve of muscle fiber area was presented as ＂S＂ shape. The diameter, perimeter and area of Iongissimus dorsi muscle and thigh muscle fibers in the reciprocal combination of Yorkshire pig ♀ × Erhualian pig♂ were higher than those in the reciprocal combination of Erhualian pig ♀ × Yorkshire pig♂ （P 〈 0.01 ）. The thigh muscle grew faster than Iongissimus dorsi muscle at the age of 20 -120 d; but at the age of 120 -180 d, the growth rate of Iongissimus dorsi muscle was higher than that of thigh muscle; and at the age of 180 d, the muscle fiber area had no significant difference between the Iongissimus dorsi muscle and the thigh muscle. The muscle fiber area had extremely significant difference between the reciprocal combinations at the age of 20 d; but no significant difference was found at the age of 180 d. [ Conclusion] The reciprocal combinations can affect the muscle fiber growth of hybrid pigs of Yorkshire pig and Erhualian pig, but the affect degree reduces with the increasing age.

Comparisons of carcass traits,meat quality,and serum metabolome between Shaziling and Yorkshire pigs

This study aims to compare the meat quality of Shaziling and Yorkshire pigs and to find the potential indicator in serum for superior meat quality.Six Shaziling and Yorkshire pigs at 30,60,90,150,210,and 300 d of age were selected to examine carcass traits,meat quality,and serum metabolome.The results showed that the body weight,carcass length,and loin eye area of Shaziling pigs at 150,210,and 300 d of age were significantly lower than those of Yorkshire pigs(P<0.05).Shaziling pigs at 150 and 300 d of age had significantly lower backfat thickness than Yorkshire pigs(P<0.05).Compared with Yorkshire pigs,Shaziling pigs at all 6 ages had a lower lean percentage and a higher fat percentage(P<0.05).At 60,90,and 150 d of age,the post-mortem pH-decline,b*value(yellowness),and drip loss of Shaziling pigs were significantly lower than those of Yorkshire pigs(P<0.05).Moreover,at 150 d of age,Shaziling pigs had significantly higher a^(*)value(redness)and intramuscular fat(IMF)content than Yorkshire pigs(P<0.05).Correlation analysis between the top 40 metabolites and phenotypes indicated that L-carnitine had positive correlations with fat percentage,pH24h,and IMF content,but had negative correlations with lean percentage,L^(*)value(lightness),and b^(*)value(P<0.05).Serum L-carnitine content,fat percentage,pH24h,and IMF content all decreased first and then increased as the pigs grew,which verified the positive correlations between L-carnitine and these phenotypes.In conclusion,Shaziling pigs have a slower growth rate but a better meat quality than Yorkshire pigs.The meat quality of Shaziling pigs is the best from 150 to 210 d of age.This study suggests that a higher serum L-carnitine content is a promising indicator for better meat quality.

Comparisons of blood biochemical parameters,digestive enzyme activities and volatile fatty acid profile between Meishan and Yorkshire piglets

This study was conducted to compare physiological characteristics between Meishan and Yorkshire piglets in their early lives. Six healthy purebred Meishan sows and Yorkshire sows with close farrowing dates were used in this research. The piglets sucked their respective sow’s milk for 14 days, then they were slaughtered to collect samples of blood, pancreas, contents of stomach, jejunum, cecum, colon as well as feces for analysis of blood biochemical parameters, digestive enzymes, and volatile fatty acid(VFA). The results showed that Yorkshire piglets had higher concentrations of high-density lipoprotein cholesterol(HDL-C) and total cholesterol(TC)(P < 0.05). Gastric lipase activity was higher in Meishan piglets but Yorkshire piglets had higher lactase activity(P < 0.05). The total VFA together with acetate and propionate in cecum and colon were higher in Meishan piglets than in Yorkshire piglets(P < 0.05),but acetate in jejunum and ratio of acetate to propionate in colon were lower in Meishan piglets than in Yorkshire piglets(P < 0.05). In conclusion, in early suckling period, significant differences exist in host metabolism and intestinal microbial metabolism between Meishan and Yorkshire piglets.

约克夏YORKSHIRE移动的璀璨宝石

小巧精致的可爱脸孔.闪耀丝缎般光彩的被毛,温柔婉约的气质.水汪汪的明亮大眼睛,总是仿佛想要诉说千言万语般地注视主人。它以其迷人浪漫的外表与聪明爱娇的个性,让许多主人在第一眼看见时就为之倾心不已。

脂质体介导pBK－Signal－EGF真核表达质粒治疗猪深Ⅱ度创面

目的：探索创面局部治疗新途径。方法：在Ｙｏｒｋｓｈｉｒｅ猪背部均匀制备圆形深Ⅱ度烫伤创面，将脂质体ＬｉｐｏｆｅｃｔＡＭＩＮＥ和ｐＢＫ－Ｓｉｇｎａｌ－ＥＧＥ．质粒混合液按倍比剂量分３组进行痂下注射，对照组注射等量Ｏｐｔｉ－ＭＥＭ减血清培养液。治疗后第９天，取创面组织做病理活检及流式细胞仪检测。结果：（１）病理活检结果表明治疗组创面毛囊周围组织上皮化程度校对照组显著加快；（２）流式细胞仪分析显示治疗组创面组织细胞的Ｓ期与Ｇ２＋Ｍ期的百分比值较对照组显著增高，治疗组间无显著差异。结论：脂质体ＬｉｐｏｆｅｃｔＡＭＩＮＥ介导ｐＢＫ－Ｓｉｇｎａｌ－ＥＧＦ真核表达质粒治疗猪深Ⅱ度创面，可促进烧伤创面的愈合。

Fattening Effects of Two Kinds of Three-way Crossbreeding Commercial Pigs

To understand the fattening performance of Duroc x Landrace×Yorkshire and Duroc×Yorkshire x landrace, 48 individuals of two kinds of three-way crossbreeding lean type commercial pigs were selected to determine average daily feed intake, average daily gain and feed gain ratio. The backtat thickness and eye muscle area of pigs were measured at the end of trial. The results showed that Duroc × Landraee × Yorkshire and Dttroc × Yorkshire x Landrace had similar fattening performance, and could be applied under scientific management conditions. The study is of great significance for development of pig industry.

Study on PRRSV Receptor Genes Differential Expression in Lung Tissues in Different Breeds of Pigs after Infecting with HP-PRRSV

[ Objective] In order to study the susceptibility molecular mechanism of highly pathogenic porcine reproductive and respiratory syndrome virus （ HP- PRRSV） JXA1 isolate on Tibetan pig, Zangmei pig and Yorkshire pig. [ Method ] In the study, real-time quantitative RT-PCR method was established to compare and analyze the differential expression of five porcine reproductive and respiratory syndrome virus （PRRSV） receptor genes （HSPG2, SIGLEC1, CD163, VIM and NMMHC-H A） in lung tissues in Tibetan pig, Zangmei pig and Yorkshire pig before the challenge and at the 4th ,7th and 14th days after the challenge with JXAI isolate. [ Results ] HSPG2 expression in Tibetan pig lung tissues increased significantly at the 4th and 14th days after the challenge with JXAI （ P 〈 0.05 ）, while decreased significantly at the 7th day after the challenge （P 〈 0.05 ）, HSPG2 expression in Zangmei pig lung tissues increased significantly at the 14th day after the challenge （P〈0.05）. SIGLECl expression in Tibetan pig lung tissues increased significantly at the 4th and 14th days after the infection（P 〈 0.05 ）, while SIGLEC 1 expression in Yorkshire pig decreased significantly at the 4th, 7th and 14th days after the challenge （P 〈0. 05 ）. CD163 expression in lung tissues of Tibetan pig and Zangmei pig both increased significantly at the 14th day after the challenge （P 〈 0.05 ）, while CD163 expression in lung tissues of Yorkshire pig decreased significantly at the 7th and 14th days after the challenge （ P 〈 0. 05 ）. VIM expression in lung tissues of Tibetan pig increased significantly at the 7th day after the challenge （ P 〈 0. 05 ）, while which of Yorkshire pig at the 7th day after the challenge decreased significantly （ P 〈 0. 05 ）. NMMHC-II A expression in lung tissues of Zangmei pig increased significantly at the 4th day after the challenge （ P 〈 0. 05 ）, and which of Yorkshire pig increased significantly at the 4th and 14th days after the challenge （P 〈 0. 05 ）. [ Conclusion] SIGLEC1 and VIM genes might be the potential key genes affecting the susceptibility of JXA1 isolate on Tibetan pig, Zangrnei pig and Yorkshire pig. Key words JXA1 isolate; Tibetan pig; Zangmei pig; Yorkshire pig; Porcine reproductive and respiratory syndrome virus receptor genes; Differential expression

Yorkshiire加入第一流联盟

在过去的几年里，所谓‘传统’染料生产领域发生了剧变。出现了合并、接管和退出竞争的现象。十年前大约有八个主要厂商，到今年底将仅留半数。