Respective characteristics of natural sea water,condensed sea water and well salt water are analyzed in this article,which shows the well salt water is the lightest polluted,condensed sea water secondly,and natural se...Respective characteristics of natural sea water,condensed sea water and well salt water are analyzed in this article,which shows the well salt water is the lightest polluted,condensed sea water secondly,and natural sea water is heaviest one. Compared with these three kinds of water coming from different place,ion c ontent is very different.So an ion content analysis for breeding water is sugges ted,meanwhile a balanced proportion is noted also.展开更多
2022年于卫氏并殖吸虫流行区湖北兴山县和保康县分别采集34只和27只溪蟹,捣碎后用NaOH消化法提取DNA。根据卫氏并殖吸虫5.8S核糖体RNA序列,使用Primer Explorer V5软件设计卫氏并殖吸虫环介导等温扩增(LAMP)特异引物,建立卫氏并殖吸虫L...2022年于卫氏并殖吸虫流行区湖北兴山县和保康县分别采集34只和27只溪蟹,捣碎后用NaOH消化法提取DNA。根据卫氏并殖吸虫5.8S核糖体RNA序列,使用Primer Explorer V5软件设计卫氏并殖吸虫环介导等温扩增(LAMP)特异引物,建立卫氏并殖吸虫LAMP检测方法,评价该方法的特异性、灵敏度、扩增效率及现场应用效果,并与沉淀镜检法比较。结果显示,建立的LAMP检出限可达1个卫氏并殖吸虫囊蚴,且与日本血吸虫、华支睾吸虫、肝片形吸虫、肝毛细线虫、钩虫等虫种无交叉反应。检测4份卫氏并殖吸虫囊蚴DNA样品,出现浊度时间(Tt值)和浊度速率峰值(Df)均值为29.0 min和0.237。LAMP检出兴山和保康县卫氏并殖吸虫阳性溪蟹分别为24只和0只,阳性率分别为70.6%(24/34)和0(0/27);沉淀镜检法检出囊蚴阳性溪蟹分别为23只和0只,阳性率分别为67.6%(23/34)和0(0/27),两种方法结果差异无统计学意义(χ^(2)=0.2,P>0.05)。建立的卫氏并殖吸虫LAMP检测方法操作简便、特异性强、灵敏度高,可用于淡水蟹卫氏并殖吸虫流行区现场调查或筛查。展开更多
文摘Respective characteristics of natural sea water,condensed sea water and well salt water are analyzed in this article,which shows the well salt water is the lightest polluted,condensed sea water secondly,and natural sea water is heaviest one. Compared with these three kinds of water coming from different place,ion c ontent is very different.So an ion content analysis for breeding water is sugges ted,meanwhile a balanced proportion is noted also.
文摘2022年于卫氏并殖吸虫流行区湖北兴山县和保康县分别采集34只和27只溪蟹,捣碎后用NaOH消化法提取DNA。根据卫氏并殖吸虫5.8S核糖体RNA序列,使用Primer Explorer V5软件设计卫氏并殖吸虫环介导等温扩增(LAMP)特异引物,建立卫氏并殖吸虫LAMP检测方法,评价该方法的特异性、灵敏度、扩增效率及现场应用效果,并与沉淀镜检法比较。结果显示,建立的LAMP检出限可达1个卫氏并殖吸虫囊蚴,且与日本血吸虫、华支睾吸虫、肝片形吸虫、肝毛细线虫、钩虫等虫种无交叉反应。检测4份卫氏并殖吸虫囊蚴DNA样品,出现浊度时间(Tt值)和浊度速率峰值(Df)均值为29.0 min和0.237。LAMP检出兴山和保康县卫氏并殖吸虫阳性溪蟹分别为24只和0只,阳性率分别为70.6%(24/34)和0(0/27);沉淀镜检法检出囊蚴阳性溪蟹分别为23只和0只,阳性率分别为67.6%(23/34)和0(0/27),两种方法结果差异无统计学意义(χ^(2)=0.2,P>0.05)。建立的卫氏并殖吸虫LAMP检测方法操作简便、特异性强、灵敏度高,可用于淡水蟹卫氏并殖吸虫流行区现场调查或筛查。