Yunmai52, developed by crossing with common wheat-Haynaldia villosa6AL/6VS translocation line 92R149 as a resistant parent in 1992, was a common wheat cultivar approved and released in 2007 in Yunnan Province, China, ...Yunmai52, developed by crossing with common wheat-Haynaldia villosa6AL/6VS translocation line 92R149 as a resistant parent in 1992, was a common wheat cultivar approved and released in 2007 in Yunnan Province, China, which is characterized by high resistance to powdery mildew and stripe rust. In this study,an F_2 population derived from a cross K78S/Yunmai52 was constructed to investigate the resistance genes, where K78 S is a wheat male sterile line susceptible to powdery mildew and stripe rust. Phenotypic identification of the parents, F_1 and F_2 populations and chi-square analyses showed that F_1 population was immune to stripe rust and powdery mildew; the segregation ratio of resistance and susceptibility to powdery mildew（χ~2=1.10χ~2_（1,0.05）=3.84） and stripe rust（χ~2=0.15χ~2_（1,0.05）=3.84） fit to a 3：1 ratio in F_2 population, indicating that Yunmai52 harbors a dominant stripe rust resistance gene and a dominant powdery mildew resistance gene. The individuals were further detected with a marker co-segregated with Pm21（SCAR_（1400）） and two markers closely linked with Yr26（XWe173 and Xbarc181）. The results showed that polymorphic bands could be amplified between the parents and between resistance and susceptibility gene pools at the same locus. Randomly 96 individuals of F_2 population were selected for verification. The results showed that the phenotype was significantly correlated with the genotype. The detection accuracy of markers SCAR_（1400）, XWe173 and Xbarc181 was 100%, 97.91% and 92.70%, respectively.Yunmai52 harbored powdery mildew resistance gene Pm21 and stripe rust resistance gene Yr26, which were both derived from 6AL/6VS translocation line 92R149.In addition, the results also demonstrate that Pm21 and Yr26 are two genes conferring durable resistance to powdery mildew and stripe rust in wheat.展开更多
[Objective] This study was tween yield-related traits and yield of conducted to understand the relationship be- Yunmai 52 which is a high-quality high-yield multi-resistant new wheat variety, and make contribution of ...[Objective] This study was tween yield-related traits and yield of conducted to understand the relationship be- Yunmai 52 which is a high-quality high-yield multi-resistant new wheat variety, and make contribution of yield-related traits to the yield of Yunmai 52 clear. [Method] Wheat variety regional trial data in Yunnan Province in 2005-2007 were subjected to correlation analysis and path analysis in the paper. [Result] Correlationanalysis showed that the yield of Yunmai 52 was in- very significant positive correlation with spikelet number per ear, maximum tiller number and grains per ear （r=0.726^＊＊, 0.717^＊＊ and 0.695^＊＊, respectively）, in signif- icant positive correlation with 1 000-grain weight （r=0.491^＊）, but in significant nega- tive correlation with sterile spikelet number per ear, and in non-significant correlation with basic seedlings, effective ears and percentage of ear bearing tillers. Partial correlation analysis showed that the yield of Yunmai 52 was in very significant posi- tive correlation with spikelet number per ear （r=0.711^＊＊）, significant positive correla- tion with 1 000-grain weight （r=0.641 =）, but in non-significant correlation with other 6 traits. Path analysis showed that spikelet number per ear （P=-0.595）, maximum tiller number （P=0.462） and t 000-grain weight （P=0.263） had more contribution to yield of Yunmai 52. [Conclusion] Therefore, in extension and application of Yunmal 52 that is a high-quality high-yield multi-resistant new wheat variety, supply of fertilizer and water should be increased in tillering stage and jointing stage, to ensure its characteristics of high tilledng ability and large ear, as well as high 1 000-grain weight, and coordinated development of other yield-related traits is beneficial to im- provement of yield of Yunmai 52.展开更多
基金Supported by National 863 Program of China(2011AA10A106)Director Fund of the Institute of Food Crops+1 种基金Yunnan Academy of Agricultural Sciences(2013LZS003)Program for Science and Technology Innovation Talents of Yunnan Province(2012HC008)~~
文摘Yunmai52, developed by crossing with common wheat-Haynaldia villosa6AL/6VS translocation line 92R149 as a resistant parent in 1992, was a common wheat cultivar approved and released in 2007 in Yunnan Province, China, which is characterized by high resistance to powdery mildew and stripe rust. In this study,an F_2 population derived from a cross K78S/Yunmai52 was constructed to investigate the resistance genes, where K78 S is a wheat male sterile line susceptible to powdery mildew and stripe rust. Phenotypic identification of the parents, F_1 and F_2 populations and chi-square analyses showed that F_1 population was immune to stripe rust and powdery mildew; the segregation ratio of resistance and susceptibility to powdery mildew（χ~2=1.10χ~2_（1,0.05）=3.84） and stripe rust（χ~2=0.15χ~2_（1,0.05）=3.84） fit to a 3：1 ratio in F_2 population, indicating that Yunmai52 harbors a dominant stripe rust resistance gene and a dominant powdery mildew resistance gene. The individuals were further detected with a marker co-segregated with Pm21（SCAR_（1400）） and two markers closely linked with Yr26（XWe173 and Xbarc181）. The results showed that polymorphic bands could be amplified between the parents and between resistance and susceptibility gene pools at the same locus. Randomly 96 individuals of F_2 population were selected for verification. The results showed that the phenotype was significantly correlated with the genotype. The detection accuracy of markers SCAR_（1400）, XWe173 and Xbarc181 was 100%, 97.91% and 92.70%, respectively.Yunmai52 harbored powdery mildew resistance gene Pm21 and stripe rust resistance gene Yr26, which were both derived from 6AL/6VS translocation line 92R149.In addition, the results also demonstrate that Pm21 and Yr26 are two genes conferring durable resistance to powdery mildew and stripe rust in wheat.
基金Supported by National key R&D Projects(2016YFD0101603)National Planning Project Co-supported by Yunnan Province(2014GA016)Science&Technology Specific Project for Benefiting People in China(2014RA056)~~
文摘[Objective] This study was tween yield-related traits and yield of conducted to understand the relationship be- Yunmai 52 which is a high-quality high-yield multi-resistant new wheat variety, and make contribution of yield-related traits to the yield of Yunmai 52 clear. [Method] Wheat variety regional trial data in Yunnan Province in 2005-2007 were subjected to correlation analysis and path analysis in the paper. [Result] Correlationanalysis showed that the yield of Yunmai 52 was in- very significant positive correlation with spikelet number per ear, maximum tiller number and grains per ear （r=0.726^＊＊, 0.717^＊＊ and 0.695^＊＊, respectively）, in signif- icant positive correlation with 1 000-grain weight （r=0.491^＊）, but in significant nega- tive correlation with sterile spikelet number per ear, and in non-significant correlation with basic seedlings, effective ears and percentage of ear bearing tillers. Partial correlation analysis showed that the yield of Yunmai 52 was in very significant posi- tive correlation with spikelet number per ear （r=0.711^＊＊）, significant positive correla- tion with 1 000-grain weight （r=0.641 =）, but in non-significant correlation with other 6 traits. Path analysis showed that spikelet number per ear （P=-0.595）, maximum tiller number （P=0.462） and t 000-grain weight （P=0.263） had more contribution to yield of Yunmai 52. [Conclusion] Therefore, in extension and application of Yunmal 52 that is a high-quality high-yield multi-resistant new wheat variety, supply of fertilizer and water should be increased in tillering stage and jointing stage, to ensure its characteristics of high tilledng ability and large ear, as well as high 1 000-grain weight, and coordinated development of other yield-related traits is beneficial to im- provement of yield of Yunmai 52.
