[Objectives] To study the protective and recovery effects of Yupingfeng polysaccharides( YPF-P) on spleen tissue structure of acute and chronic immunocompromised mice. [Methods]First,90 mice in the protective group we...[Objectives] To study the protective and recovery effects of Yupingfeng polysaccharides( YPF-P) on spleen tissue structure of acute and chronic immunocompromised mice. [Methods]First,90 mice in the protective group were injected with cyclophosphamide to replicate the chronic immunosuppressive model,and observe the protective effects of different doses of YPF-P on the spleen; 120 mice in the recovery group were injected with cyclophosphamide continuously to replicate the acute immune injury model,to observe the effects of YPF-P on spleen recovery. Besides,YPF-P positive control group was established to observe its effects on the spleen structure and function of normal mice. Testing indicators include spleen morphology and tissue structure,body mass,and spleen index. [Results]In the protective group and the recovery group,the spleen volume of the mice with different YPF-P doses increased significantly,and lymphocytes proliferated,showing obvious structural compensation and repair,and showing a dose-effect relationship. In the positive control group,the spleen function of the normal mice was significantly enhanced,and the spleen lymphoid tissue showed significant orderly proliferation. [Conclusions]Yupingfeng polysaccharides can effectively protect the function and structure of spleen,improve acute and chronic immune damage induced by cyclophosphamide,greatly promote the recovery of spleen structure and function after immune injury,and also can significant enhance the spleen structure and function of normal mice.展开更多
[Objective] The paper was to discuss regulation and protection effect of Yupingfeng polysaccharide （YPF-P） on immunosuppressive mice. [Method] Taking immunosuppressive mice induced by 80 mg/kg cyclophosphamide as te...[Objective] The paper was to discuss regulation and protection effect of Yupingfeng polysaccharide （YPF-P） on immunosuppressive mice. [Method] Taking immunosuppressive mice induced by 80 mg/kg cyclophosphamide as test animal model, effects of different doses （100,200 and 400 mg/kg） of YPF-P on spleen index, serum IL-2 and IFN-γ levels of mice were studied. [ Result] High dose of YPF-P （400 mg/kg） could improve the spleen index of immunosuppressive mice （P 〈0.05）. YPF-P could enhance serum IL-2 and IFN-γ levels of immunosuppressive mice, and the effects in middle-dose group and low-dose group were better. YPF-P could significantly increase serum IL-2 level of normal mice （ P 〈 0.01 ）, but it had no significant effect on serum IFN-γ level of normal mice. [ Conclusion] The research provides an experimental basis for clinical application of YPF-P.展开更多
研究采用RevMan3.5软件按照Cochrane方法进行Meta循证统计分析,旨在系统评价玉屏风多糖对啮齿动物免疫功能的调节作用。通过计算机检索知网、维普、万方、Pubmed、Web of sci-ence和Science Direct数据库中有关玉屏风多糖影响啮齿动物...研究采用RevMan3.5软件按照Cochrane方法进行Meta循证统计分析,旨在系统评价玉屏风多糖对啮齿动物免疫功能的调节作用。通过计算机检索知网、维普、万方、Pubmed、Web of sci-ence和Science Direct数据库中有关玉屏风多糖影响啮齿动物机体免疫功能的相关文献,共获得311篇文献,按纳入标准筛选出14篇文献,使用RevMan3.5软件进行Meta分析。以淋巴细胞增殖刺激指数(SI)、白细胞介素-2(IL-2)、分泌型免疫球蛋白A(SIgA)、辅助性T细胞分子表面特异性标志分子/细胞毒性淋巴细胞分子表面特异性标志分子(CD4^(+)/CD8^(+))、脾脏指数和转化生长因子β1(TGF-β1)等6项为有效免疫功能评价指标,其中SI、IL-2、CD4^(+)/CD8^(+)、脾脏指数和TGF-β1这5项指标与试验对照组相比,差异显著(P<0.05),SIgA指标与试验对照组相比有提高但差异不显著(P>0.05);敏感性分析显示,采用文献具有稳定性和可靠性;发表偏倚结果显示,IL-2这项指标有偏倚性,CD4^(+)/CD8^(+)和脾脏指数这两项指标无偏倚性。结果显示:玉屏风多糖可在一定程度上提高啮齿动物机体的免疫功能,后续还需进行更多的研究来进一步验证其免疫调节作用。展开更多
基金Supported by Science and Technology Planning Project of Guangdong Province(2016A020210140)Project of Key Laboratory of Preventive Veterinary Medicine for Guangdong Provincial Department of Education(2014KTSPT037)
文摘[Objectives] To study the protective and recovery effects of Yupingfeng polysaccharides( YPF-P) on spleen tissue structure of acute and chronic immunocompromised mice. [Methods]First,90 mice in the protective group were injected with cyclophosphamide to replicate the chronic immunosuppressive model,and observe the protective effects of different doses of YPF-P on the spleen; 120 mice in the recovery group were injected with cyclophosphamide continuously to replicate the acute immune injury model,to observe the effects of YPF-P on spleen recovery. Besides,YPF-P positive control group was established to observe its effects on the spleen structure and function of normal mice. Testing indicators include spleen morphology and tissue structure,body mass,and spleen index. [Results]In the protective group and the recovery group,the spleen volume of the mice with different YPF-P doses increased significantly,and lymphocytes proliferated,showing obvious structural compensation and repair,and showing a dose-effect relationship. In the positive control group,the spleen function of the normal mice was significantly enhanced,and the spleen lymphoid tissue showed significant orderly proliferation. [Conclusions]Yupingfeng polysaccharides can effectively protect the function and structure of spleen,improve acute and chronic immune damage induced by cyclophosphamide,greatly promote the recovery of spleen structure and function after immune injury,and also can significant enhance the spleen structure and function of normal mice.
基金Supported by Natural Science Fund of Guangdong Province(S2013010012191)Science and Technology Project of Guangdong Province(2009B020307004)
文摘[Objective] The paper was to discuss regulation and protection effect of Yupingfeng polysaccharide （YPF-P） on immunosuppressive mice. [Method] Taking immunosuppressive mice induced by 80 mg/kg cyclophosphamide as test animal model, effects of different doses （100,200 and 400 mg/kg） of YPF-P on spleen index, serum IL-2 and IFN-γ levels of mice were studied. [ Result] High dose of YPF-P （400 mg/kg） could improve the spleen index of immunosuppressive mice （P 〈0.05）. YPF-P could enhance serum IL-2 and IFN-γ levels of immunosuppressive mice, and the effects in middle-dose group and low-dose group were better. YPF-P could significantly increase serum IL-2 level of normal mice （ P 〈 0.01 ）, but it had no significant effect on serum IFN-γ level of normal mice. [ Conclusion] The research provides an experimental basis for clinical application of YPF-P.
文摘研究采用RevMan3.5软件按照Cochrane方法进行Meta循证统计分析,旨在系统评价玉屏风多糖对啮齿动物免疫功能的调节作用。通过计算机检索知网、维普、万方、Pubmed、Web of sci-ence和Science Direct数据库中有关玉屏风多糖影响啮齿动物机体免疫功能的相关文献,共获得311篇文献,按纳入标准筛选出14篇文献,使用RevMan3.5软件进行Meta分析。以淋巴细胞增殖刺激指数(SI)、白细胞介素-2(IL-2)、分泌型免疫球蛋白A(SIgA)、辅助性T细胞分子表面特异性标志分子/细胞毒性淋巴细胞分子表面特异性标志分子(CD4^(+)/CD8^(+))、脾脏指数和转化生长因子β1(TGF-β1)等6项为有效免疫功能评价指标,其中SI、IL-2、CD4^(+)/CD8^(+)、脾脏指数和TGF-β1这5项指标与试验对照组相比,差异显著(P<0.05),SIgA指标与试验对照组相比有提高但差异不显著(P>0.05);敏感性分析显示,采用文献具有稳定性和可靠性;发表偏倚结果显示,IL-2这项指标有偏倚性,CD4^(+)/CD8^(+)和脾脏指数这两项指标无偏倚性。结果显示:玉屏风多糖可在一定程度上提高啮齿动物机体的免疫功能,后续还需进行更多的研究来进一步验证其免疫调节作用。
