A new gene,named as NTZDS1(GenBank accession number:EU138882),was cloned from the alabastrum of Narcissus tazetta var.chinensis through RT-PCR.The sequence consists of 1 899 bp,has an open reading frame of 1 725 bp en...A new gene,named as NTZDS1(GenBank accession number:EU138882),was cloned from the alabastrum of Narcissus tazetta var.chinensis through RT-PCR.The sequence consists of 1 899 bp,has an open reading frame of 1 725 bp encoding a polypeptide of 574 amino acids.Homology analysis showed that the deduced NTZDS1 protein was highly homologous to other ZDS proteins from different species.Phylogenetic analysis indicated that NTZDS1 was more related to ZDS of Narcissus pseudonarcissus(O 49901).NTZDS1 gene was detected in flowers,leaves and roots except scales,and expression level was especially high in flowers.Within open flowers,level was the highest in petals and higher in corana while lower in stamens and pistils.展开更多
ζ （zeta）-carotene desaturase （ZDS） is a key enzyme for carotenoid biosynthesis, demonstrating high association with the yellow pigment （YP） content in wheat grain. Cloning ZDS gene and developing functional mar...ζ （zeta）-carotene desaturase （ZDS） is a key enzyme for carotenoid biosynthesis, demonstrating high association with the yellow pigment （YP） content in wheat grain. Cloning ZDS gene and developing functional markers are important for marker-assisted selection in wheat breeding. In the present study, the full-length DNA sequence of a ZDS gene on wheat chromosome 2A, designated TaZds-A1, was cloned, with 14 exons and 13 introns, and it has an open reading frame （ORF） of 1 707 bp, encoding 568 amino acid residues. A co-dominant functional marker, YP2A-1, was designed based on the polymorphisms of two alleles at the locus, TaZds-Ala and TaZds-Alb, yielding 183- and 179-bp fragments in TaZds-Ala and TaZds-Alb genotypes, respectively. A new QTL for YP content was detected on chromosome 2A, co-segregating with the functional marker YP2A-1 and TaZds-A1; it explained 11.3% of the phenotypic variance for YP content in a doubled haploid （DH） population from Zhongyou 9507/CA9632. Among 217 Chinese wheat cultivars and advanced lines, the average grain YP content of 126 cultivars with TaZds-Alb allele was 7.8% higher than that of 91 cultivars with TaZds- Ala allele.展开更多
烟草类胡萝卜素对烟叶品质和可用性有重要作用,ζ-胡萝卜素脱氢酶(ZDS)是调控类胡萝卜素合成积累的关键酶之一。为研究ZDS基因干扰表达对烟草类胡萝卜素生物合成的影响,从贵州省烟草科学研究院福泉试验基地取样品,采用不同株系相同部位...烟草类胡萝卜素对烟叶品质和可用性有重要作用,ζ-胡萝卜素脱氢酶(ZDS)是调控类胡萝卜素合成积累的关键酶之一。为研究ZDS基因干扰表达对烟草类胡萝卜素生物合成的影响,从贵州省烟草科学研究院福泉试验基地取样品,采用不同株系相同部位的方式取样,与贵烟1号(GY1)对照,筛选出能够构建RNA干扰(RNAi)载体并成功转化的转基因植株,建立ZDS基因的SYBR Green I实时荧光定量RT-PCR的检测方法,检测新鲜烟叶中ZDS基因表达水平并分析其遗传表型的变化。结果表明,ZDS和其他类胡萝卜素合成关键酶基因的表达均下调,其遗传后代出现异于正常植株的白化与矮化的表型。证明了ZDS基因对产生这种异同发挥了作用,也充分佐证了RNAi技术在基因功能分析中有着重要的作用,但这些样品表现出白化和矮化的表型是否是由于烟草类胡萝卜素的合成受到抑制尚不确定,还需要进一步验证。展开更多
文摘A new gene,named as NTZDS1(GenBank accession number:EU138882),was cloned from the alabastrum of Narcissus tazetta var.chinensis through RT-PCR.The sequence consists of 1 899 bp,has an open reading frame of 1 725 bp encoding a polypeptide of 574 amino acids.Homology analysis showed that the deduced NTZDS1 protein was highly homologous to other ZDS proteins from different species.Phylogenetic analysis indicated that NTZDS1 was more related to ZDS of Narcissus pseudonarcissus(O 49901).NTZDS1 gene was detected in flowers,leaves and roots except scales,and expression level was especially high in flowers.Within open flowers,level was the highest in petals and higher in corana while lower in stamens and pistils.
基金supported by the National Natural Science Foundation of China (30871522 and 30830072)the National Basic Research Program of China(2009CB118300)an Earmarked Fund for the Modern Agro-Industry Technology Research System, China (CARS-3-1-3)
文摘ζ （zeta）-carotene desaturase （ZDS） is a key enzyme for carotenoid biosynthesis, demonstrating high association with the yellow pigment （YP） content in wheat grain. Cloning ZDS gene and developing functional markers are important for marker-assisted selection in wheat breeding. In the present study, the full-length DNA sequence of a ZDS gene on wheat chromosome 2A, designated TaZds-A1, was cloned, with 14 exons and 13 introns, and it has an open reading frame （ORF） of 1 707 bp, encoding 568 amino acid residues. A co-dominant functional marker, YP2A-1, was designed based on the polymorphisms of two alleles at the locus, TaZds-Ala and TaZds-Alb, yielding 183- and 179-bp fragments in TaZds-Ala and TaZds-Alb genotypes, respectively. A new QTL for YP content was detected on chromosome 2A, co-segregating with the functional marker YP2A-1 and TaZds-A1; it explained 11.3% of the phenotypic variance for YP content in a doubled haploid （DH） population from Zhongyou 9507/CA9632. Among 217 Chinese wheat cultivars and advanced lines, the average grain YP content of 126 cultivars with TaZds-Alb allele was 7.8% higher than that of 91 cultivars with TaZds- Ala allele.
文摘烟草类胡萝卜素对烟叶品质和可用性有重要作用,ζ-胡萝卜素脱氢酶(ZDS)是调控类胡萝卜素合成积累的关键酶之一。为研究ZDS基因干扰表达对烟草类胡萝卜素生物合成的影响,从贵州省烟草科学研究院福泉试验基地取样品,采用不同株系相同部位的方式取样,与贵烟1号(GY1)对照,筛选出能够构建RNA干扰(RNAi)载体并成功转化的转基因植株,建立ZDS基因的SYBR Green I实时荧光定量RT-PCR的检测方法,检测新鲜烟叶中ZDS基因表达水平并分析其遗传表型的变化。结果表明,ZDS和其他类胡萝卜素合成关键酶基因的表达均下调,其遗传后代出现异于正常植株的白化与矮化的表型。证明了ZDS基因对产生这种异同发挥了作用,也充分佐证了RNAi技术在基因功能分析中有着重要的作用,但这些样品表现出白化和矮化的表型是否是由于烟草类胡萝卜素的合成受到抑制尚不确定,还需要进一步验证。
