Drought is a major abiotic stress limiting agricultural crops production worldwide.In our study,we isolated a novel C2H2-type zinc finger protein gene ZF2 from chickpea.ZF2 consisted of 232 amino acids with two QALGGH...Drought is a major abiotic stress limiting agricultural crops production worldwide.In our study,we isolated a novel C2H2-type zinc finger protein gene ZF2 from chickpea.ZF2 consisted of 232 amino acids with two QALGGH motifs in Cys2/His2 zinc finger domain.Transient expression analysis of ZF2:GFP fusion protein showed that ZF2 was a nuclear localized protein.In the yeast assay system,the full-length of ZF2 did not show transcriptional activation.Expression of ZF2 gene was enhanced by treatments of several abiotic stresses and phytohormones.The promoter region of ZF2 contained multiple stress-and hormone-related cis-elements.Overexpression of ZF2 in Arabidopsis significantly improved the root length and fresh weight at seedling stage and enhanced the survival rates and proline content under drought treatment.These results indicated that ZF2 functioned as a positive regulator in drought response.展开更多
研究了(/%)0.023Al_(sol)、0.035Al_(sol)、0.018～0.019Al_(sol)-0.012～0.014Ti和0.019～0.020Al_(sol)-0.031～0.032Nb微合金化对60 t BOF-LF-VD-300 mm×360 mm CC-连轧工艺生产的Φ120 mm ZF7渗碳钢(/%:0.18～0.20C、0.22～0.2...研究了(/%)0.023Al_(sol)、0.035Al_(sol)、0.018～0.019Al_(sol)-0.012～0.014Ti和0.019～0.020Al_(sol)-0.031～0.032Nb微合金化对60 t BOF-LF-VD-300 mm×360 mm CC-连轧工艺生产的Φ120 mm ZF7渗碳钢(/%:0.18～0.20C、0.22～0.25Si、1.22～1.30Mn、0.008～0.016P、0.019～0.029S、1.19～1.25Cr、0.001 8～0.002 2B)930℃加热时晶粒度混晶的影响。结果表明,在930℃7.5 h热处理条件下,当Al_(sol)为0.035%时ZF7钢未发生混晶现象,当钢中(/%)0.023Al_(sol)或0.018～0.019Al_(sol)-0.012～0.014Ti或0.019～0.020Al_(sol)-0.031～0.032Nb微合金化时,ZF7钢均发生了混晶现象,说明钢中有足够的Al_(sol)含量是防止ZF7钢发生混晶的关键因素。展开更多
基金sponsored by the National Natural Science Foundation of China(31160306 and 30860152)Natural Science Foundation of Zhejiang Province(LQ20C130003)+1 种基金Scientific Research Fund of Zhejiang Provincial Education Department(Y202145972 and Y202248468)Zhejiang Students’Technology and Innovation Program(Xinmiao Program)(2022R485A003).
文摘Drought is a major abiotic stress limiting agricultural crops production worldwide.In our study,we isolated a novel C2H2-type zinc finger protein gene ZF2 from chickpea.ZF2 consisted of 232 amino acids with two QALGGH motifs in Cys2/His2 zinc finger domain.Transient expression analysis of ZF2:GFP fusion protein showed that ZF2 was a nuclear localized protein.In the yeast assay system,the full-length of ZF2 did not show transcriptional activation.Expression of ZF2 gene was enhanced by treatments of several abiotic stresses and phytohormones.The promoter region of ZF2 contained multiple stress-and hormone-related cis-elements.Overexpression of ZF2 in Arabidopsis significantly improved the root length and fresh weight at seedling stage and enhanced the survival rates and proline content under drought treatment.These results indicated that ZF2 functioned as a positive regulator in drought response.
文摘研究了(/%)0.023Al_(sol)、0.035Al_(sol)、0.018～0.019Al_(sol)-0.012～0.014Ti和0.019～0.020Al_(sol)-0.031～0.032Nb微合金化对60 t BOF-LF-VD-300 mm×360 mm CC-连轧工艺生产的Φ120 mm ZF7渗碳钢(/%:0.18～0.20C、0.22～0.25Si、1.22～1.30Mn、0.008～0.016P、0.019～0.029S、1.19～1.25Cr、0.001 8～0.002 2B)930℃加热时晶粒度混晶的影响。结果表明,在930℃7.5 h热处理条件下,当Al_(sol)为0.035%时ZF7钢未发生混晶现象,当钢中(/%)0.023Al_(sol)或0.018～0.019Al_(sol)-0.012～0.014Ti或0.019～0.020Al_(sol)-0.031～0.032Nb微合金化时,ZF7钢均发生了混晶现象,说明钢中有足够的Al_(sol)含量是防止ZF7钢发生混晶的关键因素。
