Liver Transplantation (in Chinese), revised edition, edited by Professor Shu-Sen Zheng and prefaced by Professor Guo-Wei Sang, has just been published. The revised edition is mainly based on the clinical practice of t...Liver Transplantation (in Chinese), revised edition, edited by Professor Shu-Sen Zheng and prefaced by Professor Guo-Wei Sang, has just been published. The revised edition is mainly based on the clinical practice of the Liver Transplant Center, the First Affiliated Hospital, Zhejiang University and the experience展开更多
After Wang Yangming experienced the revelation of Confucian philosophy at Longchang,he disseminated his heart-mind doctrine by engaging in teaching.A great number of scholars in Guizhou followed him and formed the Qia...After Wang Yangming experienced the revelation of Confucian philosophy at Longchang,he disseminated his heart-mind doctrine by engaging in teaching.A great number of scholars in Guizhou followed him and formed the Qian sub-school of the heartmind philosophy,the earliest of local Yangming sub-schools in the country. roughout the Zhengde and Wanli periods of the Ming dynasty,Yangming scholars and local o cials in favor of the heart-mind philosophy worked in collaboration to collate and print six books of Wang’s works in Guizhou.Among these,A New Supplementary Edition of Master Wang Yangming’s Writings represents the most fruitful e ort by both government o cials(Wang Xing)and local scholars(Chen Wenxue and Ye Wu).As an o print block-carving edition of Wang Yangming’s collections in the early years,it is extremely treasured for its rarity of edition and historical value. e profusion of printing of Wang’s works in Guizhou re ects the wide circulation of his heart-mind doctrines in China’s southwest frontier regions,vividly mirroring the growth of Wang’s philosophy outside cultural centers as well as the complicated history of interactive in uence between Chinese marginal and central cultures.展开更多
The molecular scalpel of clustered regularly interspersed short palindromic repeats/CRISPR associated protein 9(CRISPR/Cas9) technology may be sharp enough to begin cutting the genes implicated in inflammatory bowel d...The molecular scalpel of clustered regularly interspersed short palindromic repeats/CRISPR associated protein 9(CRISPR/Cas9) technology may be sharp enough to begin cutting the genes implicated in inflammatory bowel disease(IBD) and consequently decrease the 6.3 billion dollar annual financial healthcare burden in the treatment of IBD. For the past few years CRISPR technology has drastically revolutionized DNA engineering and biomedical research field. We are beginning to see its application in gene manipulation of sickle cell disease,human immunodeficiency virus resistant embryologic twin gene modification and IBD genes such as Gatm(Glycine amidinotransferase, mitochondrial),nucleotide-binding oligomerization domain-containing protein 2, KRT12 and other genes implicated in adaptive immune convergence pathways have been subjected to gene editing, however there are very few publications. Furthermore,since Crohn's disease and ulcerative colitis have shared disease susceptibility and share genetic gene profile, it is paramount and is more advantageous to use CRISPR technology to maximize impact. Although, currently CRISPR does have its limitations due to limited number of specific Cas enzymes, off-target activity,protospacer adjacent motifs and crossfire between different target sites. However,these limitations have given researchers further insight on how to augment and manipulate enzymes to enable precise gene excision and limit crossfire between target sites.展开更多
We are entering an exciting epoch in livestock biotechnology during which the fundamental approaches(such as transgenesis, spermatozoa cryopreservation and artificial insemination) will be enhanced based on the modern...We are entering an exciting epoch in livestock biotechnology during which the fundamental approaches(such as transgenesis, spermatozoa cryopreservation and artificial insemination) will be enhanced based on the modern understanding of the biology of spermatogonial stem cells(SSCs) combined with the outstanding recent advances in genomic editing technologies and in vitro cell culture systems. The general aim of this review is to outline comprehensively the promising applications of SSC manipulation that could in the nearest future find practical application in livestock breeding. Here, we will focus on 1) the basics of mammalian SSC biology;2) the approaches for SSC isolation and purification;3) the available in vitro systems for the stable expansion of isolated SSCs;4) a discussion of how the manipulation of SSCs can accelerate livestock transgenesis;5) a thorough overview of the techniques of SSC transplantation in livestock species(including the preparation of recipients for SSC transplantation,the ultrasonographic-guided SSC transplantation technique in large farm animals, and the perspectives to improve further the SSC transplantation efficiency), and finally, 6) why SSC transplantation is valuable to extend the techniques of spermatozoa cryopreservation and/or artificial insemination. For situations where no reliable data have yet been obtained for a particular livestock species, we will rely on the data obtained from studies conducted in rodents because the knowledge gained from rodent research is translatable to livestock species to a great extent. On the other hand, we will draw special attention to situations where such translation is not possible.展开更多
During March 14th-16th,a combined show including Intertextile Shanghai Apparel Fabrics-Spring Edition,2018 China International Clothing&Accessories Fair(CHIC)(Spring Edition),Intertextile Shanghai Home Textiles-Sp...During March 14th-16th,a combined show including Intertextile Shanghai Apparel Fabrics-Spring Edition,2018 China International Clothing&Accessories Fair(CHIC)(Spring Edition),Intertextile Shanghai Home Textiles-Spring Edition,Yarn Expo Spring and PH Value-Spring Edition was staged in National Exhibition and Convention Center(Shanghai),covering nearly 400,000m2 exhibition area and gathering over 5,300 exhibitors to create the industry’s biggest spring/summer gathering of the entire supply chain.展开更多
Compilation of the Pharmacopoeia of the People’s Republic of China (known as the Chinese Pharmacopoeia 2010) has been completed by the Ninth Chinese Pharmacopoeia Commission,which has been officially distributed in J...Compilation of the Pharmacopoeia of the People’s Republic of China (known as the Chinese Pharmacopoeia 2010) has been completed by the Ninth Chinese Pharmacopoeia Commission,which has been officially distributed in January 2010 and implemented展开更多
文摘Liver Transplantation (in Chinese), revised edition, edited by Professor Shu-Sen Zheng and prefaced by Professor Guo-Wei Sang, has just been published. The revised edition is mainly based on the clinical practice of the Liver Transplant Center, the First Affiliated Hospital, Zhejiang University and the experience
文摘After Wang Yangming experienced the revelation of Confucian philosophy at Longchang,he disseminated his heart-mind doctrine by engaging in teaching.A great number of scholars in Guizhou followed him and formed the Qian sub-school of the heartmind philosophy,the earliest of local Yangming sub-schools in the country. roughout the Zhengde and Wanli periods of the Ming dynasty,Yangming scholars and local o cials in favor of the heart-mind philosophy worked in collaboration to collate and print six books of Wang’s works in Guizhou.Among these,A New Supplementary Edition of Master Wang Yangming’s Writings represents the most fruitful e ort by both government o cials(Wang Xing)and local scholars(Chen Wenxue and Ye Wu).As an o print block-carving edition of Wang Yangming’s collections in the early years,it is extremely treasured for its rarity of edition and historical value. e profusion of printing of Wang’s works in Guizhou re ects the wide circulation of his heart-mind doctrines in China’s southwest frontier regions,vividly mirroring the growth of Wang’s philosophy outside cultural centers as well as the complicated history of interactive in uence between Chinese marginal and central cultures.
文摘The molecular scalpel of clustered regularly interspersed short palindromic repeats/CRISPR associated protein 9(CRISPR/Cas9) technology may be sharp enough to begin cutting the genes implicated in inflammatory bowel disease(IBD) and consequently decrease the 6.3 billion dollar annual financial healthcare burden in the treatment of IBD. For the past few years CRISPR technology has drastically revolutionized DNA engineering and biomedical research field. We are beginning to see its application in gene manipulation of sickle cell disease,human immunodeficiency virus resistant embryologic twin gene modification and IBD genes such as Gatm(Glycine amidinotransferase, mitochondrial),nucleotide-binding oligomerization domain-containing protein 2, KRT12 and other genes implicated in adaptive immune convergence pathways have been subjected to gene editing, however there are very few publications. Furthermore,since Crohn's disease and ulcerative colitis have shared disease susceptibility and share genetic gene profile, it is paramount and is more advantageous to use CRISPR technology to maximize impact. Although, currently CRISPR does have its limitations due to limited number of specific Cas enzymes, off-target activity,protospacer adjacent motifs and crossfire between different target sites. However,these limitations have given researchers further insight on how to augment and manipulate enzymes to enable precise gene excision and limit crossfire between target sites.
基金supported by the S grant of the Ministry of Education,Youth and Sport(MEYS)of Czech Republicsupported by the Primus Research Programme PRIMUS/17/MED/16 of the Charles University
文摘We are entering an exciting epoch in livestock biotechnology during which the fundamental approaches(such as transgenesis, spermatozoa cryopreservation and artificial insemination) will be enhanced based on the modern understanding of the biology of spermatogonial stem cells(SSCs) combined with the outstanding recent advances in genomic editing technologies and in vitro cell culture systems. The general aim of this review is to outline comprehensively the promising applications of SSC manipulation that could in the nearest future find practical application in livestock breeding. Here, we will focus on 1) the basics of mammalian SSC biology;2) the approaches for SSC isolation and purification;3) the available in vitro systems for the stable expansion of isolated SSCs;4) a discussion of how the manipulation of SSCs can accelerate livestock transgenesis;5) a thorough overview of the techniques of SSC transplantation in livestock species(including the preparation of recipients for SSC transplantation,the ultrasonographic-guided SSC transplantation technique in large farm animals, and the perspectives to improve further the SSC transplantation efficiency), and finally, 6) why SSC transplantation is valuable to extend the techniques of spermatozoa cryopreservation and/or artificial insemination. For situations where no reliable data have yet been obtained for a particular livestock species, we will rely on the data obtained from studies conducted in rodents because the knowledge gained from rodent research is translatable to livestock species to a great extent. On the other hand, we will draw special attention to situations where such translation is not possible.
文摘During March 14th-16th,a combined show including Intertextile Shanghai Apparel Fabrics-Spring Edition,2018 China International Clothing&Accessories Fair(CHIC)(Spring Edition),Intertextile Shanghai Home Textiles-Spring Edition,Yarn Expo Spring and PH Value-Spring Edition was staged in National Exhibition and Convention Center(Shanghai),covering nearly 400,000m2 exhibition area and gathering over 5,300 exhibitors to create the industry’s biggest spring/summer gathering of the entire supply chain.
文摘Compilation of the Pharmacopoeia of the People’s Republic of China (known as the Chinese Pharmacopoeia 2010) has been completed by the Ninth Chinese Pharmacopoeia Commission,which has been officially distributed in January 2010 and implemented
