Glycosmis longipetala F. J. Mou ＆ D. X. Zhang is described from Guangxi and Yunnan provinces in southwestern China. The new species is similar to G. cochinchinensis （Lour.） Pierre ex Engl. by its simple leaves, but...Glycosmis longipetala F. J. Mou ＆ D. X. Zhang is described from Guangxi and Yunnan provinces in southwestern China. The new species is similar to G. cochinchinensis （Lour.） Pierre ex Engl. by its simple leaves, but distinguishable in having long-elliptic or oblanceolate （vs. ovate） leaves, long-ovoid to ellipsoid （vs. ovoid） floral buds, ovaries with many tubercles （vs. smooth） and glabrous （vs. pubescent） stamens. The pollen grains of the new species are 23.9±3.09 （20.8-27.0）×22.0±1.80 （20.4-24.4）μm in size with reticulate exine ornamentation in equatorial area and foveolate in polar area. The chromosome number of the new species is 2n=72.展开更多
The definition of relatively stable expressed internal reference genes is essential in both traditional blotting quantification and as a modern data quantitative strategy.Appropriate internal reference genes can accur...The definition of relatively stable expressed internal reference genes is essential in both traditional blotting quantification and as a modern data quantitative strategy.Appropriate internal reference genes can accurately standardize the expression abundance of target genes to avoid serious experimental errors.In this study,the expression profiles of ten candidate genes,ACT1,ACT2,GAPDH,eIF1,eIF2,α-TUB,β-TUB,TBP,RNA Pol II and RP II,were calculated for a suitable reference gene selection in Paeonia ostii T.Hong et J.X.Zhang leaves under various drought stress conditions.Data were processed by the four regularly used evaluation software.A comprehensive analysis revealed that RNA Pol II was the most stable gene and eIF2 was the least stable one.In addition,the geNorm program provided the optimal choice of two reference gene combination,RNA Pol II andβ-TUB,for qRT-PCR normalization in P.ostii subjected to different drought stress levels.Our research provided convenience for gene expression analysis in P.ostii under drought stress and promoted research of effective methods to alleviate P.ostii drought stress in the future.展开更多
开展了凤丹牡丹(Paeonia ostii T.Hong et J.X.Zhang)种子的特性观测并进行了种子分级试验;采用不同浸种温度、不同浸种时间、沙藏层积、不同浓度激素处理凤丹牡丹种子,筛选出能够促进种子萌发的最佳处理方式。结果表明,利用凤丹种子的...开展了凤丹牡丹(Paeonia ostii T.Hong et J.X.Zhang)种子的特性观测并进行了种子分级试验;采用不同浸种温度、不同浸种时间、沙藏层积、不同浓度激素处理凤丹牡丹种子,筛选出能够促进种子萌发的最佳处理方式。结果表明,利用凤丹种子的比重不同,采取水浸法进行种子分级,能有效区分种子优劣,且Ⅱ级种子的发芽率最高,达到99%;赤霉素(GA3)处理能大幅提高播种出苗率,GA3促进种子萌发的的最佳浓度为150 mg/L,种子萌发率达到99%。种子的品质与质量相关性不大,与吸水率呈反比;凤丹牡丹种子萌发需要一定的生理后熟期。展开更多
2016年邯郸市林业局引进"凤丹"牡丹(Paeonia ostii T.Hong et J.X.zhang)进行驯化、栽培等试验。通过连续2年观测和统计,"凤丹"牡丹在邯郸适应性良好,成活率、保存率均达到85%以上,物候期相对山东菏泽市稍有延后,...2016年邯郸市林业局引进"凤丹"牡丹(Paeonia ostii T.Hong et J.X.zhang)进行驯化、栽培等试验。通过连续2年观测和统计,"凤丹"牡丹在邯郸适应性良好,成活率、保存率均达到85%以上,物候期相对山东菏泽市稍有延后,产量和收益随苗龄的增长逐年增加,生态效益和经济效益显著,适宜在邯郸各地推广。关于"凤丹"特丹稳产丰产的最佳条件和模式还需进一步深入研究。展开更多
基金supported by National Natural Science Foundation of China (30370107).
文摘Glycosmis longipetala F. J. Mou ＆ D. X. Zhang is described from Guangxi and Yunnan provinces in southwestern China. The new species is similar to G. cochinchinensis （Lour.） Pierre ex Engl. by its simple leaves, but distinguishable in having long-elliptic or oblanceolate （vs. ovate） leaves, long-ovoid to ellipsoid （vs. ovoid） floral buds, ovaries with many tubercles （vs. smooth） and glabrous （vs. pubescent） stamens. The pollen grains of the new species are 23.9±3.09 （20.8-27.0）×22.0±1.80 （20.4-24.4）μm in size with reticulate exine ornamentation in equatorial area and foveolate in polar area. The chromosome number of the new species is 2n=72.
基金This work was supported by the National Key R&D Program of China(2018YFD1000405)Jiangsu Modern Agricultural Industrial Technology System(JATS[2020]436)。
文摘The definition of relatively stable expressed internal reference genes is essential in both traditional blotting quantification and as a modern data quantitative strategy.Appropriate internal reference genes can accurately standardize the expression abundance of target genes to avoid serious experimental errors.In this study,the expression profiles of ten candidate genes,ACT1,ACT2,GAPDH,eIF1,eIF2,α-TUB,β-TUB,TBP,RNA Pol II and RP II,were calculated for a suitable reference gene selection in Paeonia ostii T.Hong et J.X.Zhang leaves under various drought stress conditions.Data were processed by the four regularly used evaluation software.A comprehensive analysis revealed that RNA Pol II was the most stable gene and eIF2 was the least stable one.In addition,the geNorm program provided the optimal choice of two reference gene combination,RNA Pol II andβ-TUB,for qRT-PCR normalization in P.ostii subjected to different drought stress levels.Our research provided convenience for gene expression analysis in P.ostii under drought stress and promoted research of effective methods to alleviate P.ostii drought stress in the future.
文摘2016年邯郸市林业局引进"凤丹"牡丹(Paeonia ostii T.Hong et J.X.zhang)进行驯化、栽培等试验。通过连续2年观测和统计,"凤丹"牡丹在邯郸适应性良好,成活率、保存率均达到85%以上,物候期相对山东菏泽市稍有延后,产量和收益随苗龄的增长逐年增加,生态效益和经济效益显著,适宜在邯郸各地推广。关于"凤丹"特丹稳产丰产的最佳条件和模式还需进一步深入研究。
