Eight Zhes Decoction ameliorates the lipid dysfunction of nonalcoholic fatty liver disease using integrated lipidomics, network pharmacology and pharmacokinetics

Nonalcoholic fatty liver disease(NAFLD)has developed into the most common chronic liver disease and can lead to liver cancer.Our laboratory previously developed a novel prescription for NAFLD,“Eight Zhes Decoction”(EZD),which has shown good curative effects in clinical practice.However,the pharmacodynamic material basis and mechanism have not yet been revealed.A strategy integrating lipidomics,network pharmacology and pharmacokinetics was used to reveal the active components and mechanisms of EZD against NAFLD.The histopathological results showed that EZD attenuated the degrees of collagen deposition and steatosis in the livers of nonalcoholic steatofibrosis model mice.Furthermore,glycerophospholipid metabolism,arachidonic acid metabolism,glycerolipid metabolism and linoleic acid metabolism with phospholipase A2 group IVA(PLA2G4A)and cytochrome P450 as the core targets and 12,13-cis-epoxyoctadecenoic acid,12(S)-hydroxyeicosatetraenoic acid,leukotriene B4,prostaglandin E2,phosphatidylcholines(PCs)and triacylglycerols(TGs)as the main lipids were found to be involved in the treatment of NAFLD by EZD.Importantly,naringenin,artemetin,canadine,and bicuculline were identified as the active ingredients of EZD against NAFLD;in particular,naringenin reduces PC consumption by inhibiting the expression of PLA2G4A and thus promotes sufficient synthesis of very-low-density lipoprotein to transport excess TGs in the liver.This research provides valuable data and theoretical support for the application of EZD against NAFLD.

汉语助词“着(zhe)”的种种译法

正在走着。/步。走着去学校。/学校。门开着。/開。

从翻译实践看“シテイル”和“着(zhe)”的不对应

"シテイル"作为持续体被列入日语的时体体系之中,自然让人联想到它和表示汉语持续体的符号"着"之间是否完全对应。鉴于《挪威的森林》日文版中含"シテイル"的句子十分丰富,作者选定该原著及其中译本(林少华版)作为展开分析的语料数据库,从对比语言学的角度来考察"シテイル"和"着"的对应情况,统计结果显示两者在绝大多数情况下并不对应,进而从中日两种语言在词汇、句法上的差别来分析不对应的根本原因。

大黄zhe虫胶囊联合力克敏治疗慢性乙肝36例

我院自2002年6月-2003年6月应用大黄zhe虫胶囊联合力克敏注射液治疗慢性乙型肝炎36例，取得良好效果，现报道如下：

现代汉语“着(·zhe)”的研究综述

＂着（·zhe）＂在现代汉语中的使用频率很高,但是对其语法意义,先哲时贤却众说纷纭,令人莫衷一是。例如表动作进行,表状态持续,表惯性,表情状等等。这种情况对于汉语信息输入和对外汉语教学都是不利的。有鉴于此,本文拟全面考察前人对＂着（·zhe）＂的语法分布和语法意义的研究,争取得出前人相关研究的成败,为今后关于＂着（·zhe）＂的研究提供一定的借鉴。

Plasma pharmacological study on Da Huang Zhe Chong capsule on platelet aggregation of normal person

Objective To develop a plasma pharmacological method evaluates the effect of Da Huang Zhe Chong capsule on platelet aggregation and its mechanism, which is a representative Traditional Chinese Medicine Patent Prescription Promoting blood circulation by removing blood stasis. Methods Platelets specimens from healthy volunteers made serum and plasma with medicine, while platelet PRP were separated, which were divided into 8groups,i.e. auto-serum, allo-serum, serum with Da Huang Zhe Chong capsule , serum with aspirin, auto-plasma, plasma with Da Huang Zhe Chong capsule, plasma with aspirin, every group added to serum and plasma to hatch. After ADP and adrenalin were added into the specimens and hatched, the effects of specimens on platelet aggregation were observed. Results After ADP adrenalin were added, all the serum groups did not present platelet aggregation,while all the plasma group presented platelet aggregation. P1, P5, Pmax, t and TM have no significant difference (P＞0. 05) between auto-plasma group and allo-plasma group induced by ADP and adrenalin. P1, P5, t and Pmax have significant differences (P＜0. 01) and TM decreased significantly (P＜0. 05) comparing plasma group with Da Huang Zhe Chong capsule and plasma group of aspirin to allo-plasma group. P1, t and Pmax have significant difference (P＜0.05), and P5 and TM are simulate comparing plasma group with Da Huang Zhe Chong capsule to plasma group of aspirin. P1, P5, t and Pmax have significant differences (P＜0. 01), P1, TM have also significant(P＜0. 05), comparing plasma group of Da Huang Zhe Chong capsule with plasma of aspirin to allo-plasma group induced by adrenalin. P1 ,P5 and Pmax have significant differences (P＜0.05), and t and Pmax are simulate comparing plasma group with Da Huang Zhe Chong capsule with plasma group of aspirin. Conclusion The serum pharmacological study is inappropriate to study platelet aggregation in vitro. The plasma pharmacological study is inappropriate to study platelet aggregation in vitro. The plasma pharmacological study could reflect the pharmacological effect produced in vivo. Da Huang Zhe Chong capsule has better anti artery thrombosis effect than aspirin, and it is an ideal medicine for anti artery thrombosis.

Developing International Economic and Technological Cooperation in Zhe Jiang

Zhejiang is a coastal province with advanced economy,solid foundation of industry and agriculture and all-comprehensive industry sectors,and with lignht industry andtextile as the mainstay Its popularized education,advancedscience and technology and ample supply of high-quality labourforce benefit itselt involving in foreign project contracting,labour export and overseas investment.Since 1979,Zhejianghas accepted more than ten foreign projects worth US$300million and supplied labour service to 40 countries。

大黄zhe虫丸外敷治疗耳鸣耳聋

我们用经方“大黄zhe虫丸”外敷治疗一例瘀血阻滞型耳鸣耳聋，效果满意介绍为下。

大黄Zhe虫丸合卵磷脂抗肝纤维化的临床研究

目的：观察中药大黄Zhe虫丸合用卵磷脂对慢性乙型肝炎肝纤维化的疗效。方法：选择慢性乙肝纤维化指标异常的患者90例，随机分成治疗组及对照组各45例，治疗组口服大黄Zhe虫丸合卵磷脂；对照组口服凯西莱，观察治疗后肝纤维化指标的变化。结果：治疗组HA、LN、PCⅢ、IV－C、ALT、AST有明显降低，而对照组各项指标下降不明显（P＜0．01、P＜0．05）。结论：大黄Zhe虫丸合卵磷脂的抗纤维化作用优于凯西莱。

Zhe9248—a new scented rice line cames out in Zhejiang

This novel rice line is developed recently’by a research team led by Associate Prof QIU Baiqin, Crop Breeding and Cultivation Institute, Zhejiang Academy of Agricultural Sciences. Zhe 9248 is of high resistance to blast, early mature and of higher yield. Being soft, smooth, and glossy, the cooked rice of "Zhe 9248" has excellent eating quality. The kernels of "Zhe 9248" are hardened to such a degree that they could be capa-

大黄蟅虫丸抗大鼠免疫性肝纤维化研究

目的：研究大黄虫丸防治肝纤维化的作用。方法：运用大鼠免疫性肝纤维化模型，观察大黄虫丸对肝组织病理学、肝羟脯氨酸（ＨＹＰ）含量的影响。结果：大黄虫丸预防和治疗试验肝纤维化率分别为７２．７％和７１．２％，而模型组和秋水仙碱组肝纤维化率为９２．３％～１００％，尤其是大黄虫丸治疗试验组肝ＨＹＰ含量下降显著，与模型组比较Ｐ＜０．０５。结论：大黄虫丸有一定的抗肝纤维化作用。

大黄虫丸对大鼠肝星状细胞表达TGF-β_1的影响

目的 :探讨大黄虫丸对大鼠肝星状细胞表达转化生长因子β1(TGF β1)的影响。方法 :用CCl4复合因素致大鼠肝纤维化模型 ,同时分别给予 3种剂量大黄虫丸治疗 ,免疫组化方法观察TGF β1、α 平滑肌肌动蛋白 (α SMA)的合成表达及纤维化程度分级。结果 :经给予大黄虫丸干预治疗常规剂量组大鼠TGF β1、α SMA表达与模型组比较差异无显著性 (P >0 0 5 ) ,但双倍剂量组和三倍剂量组TGF β1、α SMA在汇管区及不连续纤维间隔的表达明显减弱 (P <0 0 5 ,P <0 0 1)。结论 :大黄虫丸较大剂量能抑制肝星状细胞增殖和分泌TGF β1,减少胶原生成 ,从而减弱肝星状细胞的自分泌放大效应 ,这可能是大黄虫丸抗肝纤维化作用的主要机制之一。

仲景三方对大鼠肝纤维化不同时期胶原Ⅰ,Ⅲ,Ⅳ影响的观察

目的 :探讨小柴胡汤、大黄虫丸、鳖甲煎丸治疗肝纤维化机理之异同。方法 :用CCl4 制造肝纤维化模型 ,分别于造模第 1天、第 5周、第 9周时分别加入上述三方制成的混悬液 ,用药时间均为 4周 ,处死取材后HE及免疫组化染色 ,观察三方在肝纤维化不同时期对胶原Ⅰ ,Ⅲ ,Ⅳ的影响。结果 :小柴胡汤预防组及治疗Ⅰ组肝脏病理变化不明显 ,胶原Ⅰ ,Ⅲ ,Ⅳ阳性程度轻 ;治疗Ⅰ组、治疗Ⅱ组以大黄虫丸、鳖甲煎丸组病变程度轻 ,胶原Ⅰ ,Ⅲ ,Ⅳ阳性程度轻 ,但ridite分析治疗Ⅰ组大黄虫丸优于鳖甲煎丸 ;治疗Ⅱ组鳖甲煎丸优于大黄虫丸。结论 :小柴胡汤治疗肝纤维化当早期用药 ;轻度肝纤维化大黄虫丸疗效最优 ,亦可用鳖甲煎丸 ;较重的肝纤维化鳖甲煎丸疗效最优 。

大黄(庶虫)虫丸抗大鼠免疫性肝纤维化研究

目的:研究大黄廑虫丸预防和治疗肝纤维化的作用。方法:运用牛血清白蛋白致大鼠免疫损伤性肝纤维化模型,观察大廑黄虫丸对肝组织病理学、肝组织羟脯氨酸(HyP)和血清透明质酸(HA)及肝功能的影响,并设秋水仙碱作对照。结果:大黄廑虫丸预防和治疗组肝纤维化率分别为72.7％和71.4％,而模型组和秋水仙碱组肝纤维化率为92.3％～100％,尤其是大黄廑虫丸治疗试验组肝HyP含量下降最显著,与模型组比较,P<0.05。结论:大黄廑虫丸有一定的抗肝纤维化作用,尤适用于对肝纤维组织的降解。