[Objectives]To screen optimum conditions of HPLC and TLC for qualitative discrimination of berberine hydrochloride contained in Jin Weng Zhili Particle,thus providing basis for quality standard. [Methods]According to ...[Objectives]To screen optimum conditions of HPLC and TLC for qualitative discrimination of berberine hydrochloride contained in Jin Weng Zhili Particle,thus providing basis for quality standard. [Methods]According to the method described in Chinese Veterinary Pharmacopoeia,thin layer chromatography and high performance liquid chromatography were used respectively for qualitative and quantitative identification of berberine hydrochloride contained in Jin Weng Zhili Particle by improving the conditions of extraction and purification. [Results]After optimizing TLC conditions of Jin Weng Zhili Particle,the separation effect of TLC of test samples was favorable. The spot color was clear,reproducibility was good,and precision was high. Under the conditions of mobile phase constituted by acetonitrile-water-glacial acetic acid-triethylamine( 28∶ 70∶ 1∶ 1),detection wavelength of 265 nm,flow rate of 1. 0 m L min^(-1)and column temperature of 35℃,the resolution of berberine hydrochloride was good; the injection volume in the range of 3. 15-50 μg· m L^(-1)had good linear relationship with the peak area( R^2= 0. 998); the average sample recovery rate(n=6) was 5. 672%,and RSD was 0. 864%; the content of berberine hydrochloride contained in Jin Weng Zhili Particle was 4. 023 mg. [Conclusions]The method was of good feasibility,reproducibility and specificity,and could be used for qualitative and quantitative detection of berberine hydrochloride contained in Jin Weng Zhili Particle.展开更多
Zhili town is located about 15 kilometers from Huzhou City in Zhejiang.Today it is one of China’s biggest children’s apparel production bases and cashmere apparel industrial clusters.
AMONG 29 newly appointedcross-century Ministers of theState Council in 1998, ChenZhili, is one of only two women. Herappointment has caused a mild sensationamong the mass media at home and abroad,not only due to her f...AMONG 29 newly appointedcross-century Ministers of theState Council in 1998, ChenZhili, is one of only two women. Herappointment has caused a mild sensationamong the mass media at home and abroad,not only due to her female status but alsothe important role education plays inChina’s basic policy of "revitalizing thecountry through science and technology."As Minister of Education in a country witha population of 1.2 billion, Chen Zhili notonly has profound scientific and culturalknowledge, but the necessary, rich workingand life experiences as a leader. She isindeed qualified to be in charge of China’seducation in the 21 st century, according tosome media.展开更多
基金Supported by Special Project of National Public Benefit Industry(Agriculture)(201303040-05)Special Project of Science and Technology Groundwork(2013-FY110600-03)
文摘[Objectives]To screen optimum conditions of HPLC and TLC for qualitative discrimination of berberine hydrochloride contained in Jin Weng Zhili Particle,thus providing basis for quality standard. [Methods]According to the method described in Chinese Veterinary Pharmacopoeia,thin layer chromatography and high performance liquid chromatography were used respectively for qualitative and quantitative identification of berberine hydrochloride contained in Jin Weng Zhili Particle by improving the conditions of extraction and purification. [Results]After optimizing TLC conditions of Jin Weng Zhili Particle,the separation effect of TLC of test samples was favorable. The spot color was clear,reproducibility was good,and precision was high. Under the conditions of mobile phase constituted by acetonitrile-water-glacial acetic acid-triethylamine( 28∶ 70∶ 1∶ 1),detection wavelength of 265 nm,flow rate of 1. 0 m L min^(-1)and column temperature of 35℃,the resolution of berberine hydrochloride was good; the injection volume in the range of 3. 15-50 μg· m L^(-1)had good linear relationship with the peak area( R^2= 0. 998); the average sample recovery rate(n=6) was 5. 672%,and RSD was 0. 864%; the content of berberine hydrochloride contained in Jin Weng Zhili Particle was 4. 023 mg. [Conclusions]The method was of good feasibility,reproducibility and specificity,and could be used for qualitative and quantitative detection of berberine hydrochloride contained in Jin Weng Zhili Particle.
文摘Zhili town is located about 15 kilometers from Huzhou City in Zhejiang.Today it is one of China’s biggest children’s apparel production bases and cashmere apparel industrial clusters.
文摘AMONG 29 newly appointedcross-century Ministers of theState Council in 1998, ChenZhili, is one of only two women. Herappointment has caused a mild sensationamong the mass media at home and abroad,not only due to her female status but alsothe important role education plays inChina’s basic policy of "revitalizing thecountry through science and technology."As Minister of Education in a country witha population of 1.2 billion, Chen Zhili notonly has profound scientific and culturalknowledge, but the necessary, rich workingand life experiences as a leader. She isindeed qualified to be in charge of China’seducation in the 21 st century, according tosome media.
