[Objectives]To evaluate the quality of Cardamine macrophylla Willd as Tibetan and Qiang medicinal materials,so as to improve its quality standard and evaluate the quality of C.macrophylla Willd in western Sichuan Prov...[Objectives]To evaluate the quality of Cardamine macrophylla Willd as Tibetan and Qiang medicinal materials,so as to improve its quality standard and evaluate the quality of C.macrophylla Willd in western Sichuan Province.[Methods]C.macrophylla Willd produced from western Sichuan Province was used as the sample,and the contents of moisture,total ash,acid-insoluble ash,extract,total flavonoids and quercetin in the ground part of C.macrophylla Willd were determined in accordance with the methods of Chinese Pharmacopoeia(2020 edition).With the above seven indicators as evaluation indicators,the quality of medicinal materials was comprehensively evaluated by cluster analysis and principal component analysis(PCA).[Results]According to the results of each indicator,the moisture content of C.macrophylla Willd sample should not exceed 11.00%,the total ash content should not exceed 18%,the acid-insoluble ash content should not exceed 6%,the extract content should not be less than 19%,the total flavone content(calculated by quercetin)should not be less than 2%,and the quercetin content should not be less than 0.15%.[Conclusions]The sample S7 has the best quality and S6 has the worst quality.In this study,the quantitative analysis method of total flavonoids(quercetin)and quercetin in C.macrophylla Willd was established,and the limits of each indicator were preliminarily formulated.展开更多
The efficiency of 7 treatment methods for breaking dormancy in zoysiagrass ( Zoysia japonica Steud.) seeds was synchronously compared. Two methods in which seeds soaking in 30% NaOH solution for 20 or 80 minutes were ...The efficiency of 7 treatment methods for breaking dormancy in zoysiagrass ( Zoysia japonica Steud.) seeds was synchronously compared. Two methods in which seeds soaking in 30% NaOH solution for 20 or 80 minutes were the optimum for breaking dormancy of seeds due to their simple and easy operation in techniques, economic and rapid effectiveness in performance and high germination rates (77.6% and 88.0% respectively).展开更多
To rapidly obtain high-quality genomic DNA from Chenopodium quinoa Willd, the genomic DAN in different tissues （leaves, stems and roots） of Chenopodi- um quinoa Willd was extracted by modified CTAB method, SDS metho...To rapidly obtain high-quality genomic DNA from Chenopodium quinoa Willd, the genomic DAN in different tissues （leaves, stems and roots） of Chenopodi- um quinoa Willd was extracted by modified CTAB method, SDS method and high- salt Iow-pH method, respectively. The quality and yield of extracted DNA was deter- mined using agarose gel electrophoresis and UV spectrophotometry. At the same time, the PCR-SSR and SSCP molecular detection was also performed. The results showed that the gel test strips, without obvious decomposition, of all the extraction methods were relatively obvious; the genomic DNA yield extracted by modified CTAB method was highest, followed by that by SDS method, and the genomic DNA extracted by high-salt Iow-pH method was lowest： the genomic DNA yields extracted by different methods from Chenopodium quinoa Wiltd leaves were all high- er than those from roots and stems; the quality of Chenopodium quinoa Willd ge- nomic DNA extracted by modified CTAB method and high-salt Iow-pH method was better, and polyphenols, polysaccharides and other impurities were removed more completely. The PCR-SSR and SSCP detection results showed that the genomic DNA extracted by different methods from different tissues of Chenopodium quinoa Willd all could be better amplified, and high-quality strips could be obtained. So the Chenopodium quinoa Willd genomic DNA extracted by the three methods all can be used for subsequent molecular biology research.展开更多
The age-structure of natural population of Zoysia japonica in Xiuyan County of Liaoning Province was studied by generational method. The results showed that the highest tiller age class was three, but 1st age class ti...The age-structure of natural population of Zoysia japonica in Xiuyan County of Liaoning Province was studied by generational method. The results showed that the highest tiller age class was three, but 1st age class tillers held dominant posi-tion with proportions over 95% in each month during the growing seasons. The 2nd age class and 3rd age class tillers were minority in the population. So Z. japonica population was an expanding population. The zero age class buds on the rhizomes were dominant in buds age structures. The proportion of buds to tillers on quantity in each month was about 30% to 40% and reached the highest at the end of September. The increasing of buds proportion before dormancy guaranteed the quantity of tillers in the next spring. The biomass of 1st age class tillers changed with time. The biomass kept increasing from April to July and reached the highest at the end of July and then decreased.展开更多
Callus induction effects of nine varieties of Chenopodium quinoa Willd. were compared by taking stem segments and cotyledons of C. quinoa as the ex- plants. At the same time, callus JnductJon of stem segments was opti...Callus induction effects of nine varieties of Chenopodium quinoa Willd. were compared by taking stem segments and cotyledons of C. quinoa as the ex- plants. At the same time, callus JnductJon of stem segments was optimized, as well as the callus proliferation system. Research results showed that the optimal explant for callus induction was stem segment. The average callus induction rate of nine varieties reached 90% in culture medium MS ＋ 0.5 mg/L 2, 4-D. In the callus opti- mization test, treatment VI （MS ＋ 0.5 mg/L 2, 4-D ＋ 0.5 mg/L KT ＋ 0.5 mg/L NAA） and treatment II （MS ＋ 0.5 mg/L 2, 4-D） had close induction rate, but the callus morphology was greatly different. The latter had loose, glossy and yellowish white calluses. Therefore, culture medium MS ＋ 0.5 mg/L 2, 4-D was the optimal for callus induction. And using 2, 4-D together with KT and NAA could significantly increase the proliferation rate of calluses.展开更多
A new tdterpenoid glycoside, 3-β-O-β-D-glucopyranosyl presenegenin 28-O-α-L-arabipyranosyl（1 → 3）-6-β- xylopyranosyl（1 → 4）-[β-D-apiofuranosyl（1 → 3）]-Ct-L-rhamnopyranosyl（1 → 2）-[α-L-rhamnopyranosyl...A new tdterpenoid glycoside, 3-β-O-β-D-glucopyranosyl presenegenin 28-O-α-L-arabipyranosyl（1 → 3）-6-β- xylopyranosyl（1 → 4）-[β-D-apiofuranosyl（1 → 3）]-Ct-L-rhamnopyranosyl（1 → 2）-[α-L-rhamnopyranosyl（1 → 3）]-β-D-fucopyranosyl ester （1） was isolated from the Polygala tenuifolia Willd., together with two known saponins, including polygalasaponinXXIV （2） and polygalasaponinXXVIII （3）. The structure of new compound was elucidated by spectroscopic methods.展开更多
Ultrasonically assisted extraction of isoflavones from the stem of Pueraria lobata （Willd.） Ohwi has been carried out with an ultrasonic extracting apparatus （20kHz, electrical power input to the transducer in 0-6...Ultrasonically assisted extraction of isoflavones from the stem of Pueraria lobata （Willd.） Ohwi has been carried out with an ultrasonic extracting apparatus （20kHz, electrical power input to the transducer in 0-650W）. The influence of the electrical power input and extraction time on the＇extraction yield is investigated in water, n-butanol, and 95% （by volume） and 50% （by volume） ethanol aqueous solution. The experimental results indicate that the yields of total isoflavones are higher in ultrasonically assisted extraction than those obtained from con-ventional extraction.Moreover,a mathematical model is proposed,by introducing the electrical power input to index the ultrsound intensity,to describe the behavior of ultrasonically assisted extraction.It is found that the model calcuations are in good agreement with the experimental data.展开更多
基金Supported by Scientific Research Project for School-level Teachers of Sichuan College of Traditional Chinese Medicine in 2023 (23ZRYB08)Tibetan Plateau Ethnic Medicinal Resources Protection and Utilization Key Laboratory Open Fund Project of Southwest Minzu University (QTPEM2305).
文摘[Objectives]To evaluate the quality of Cardamine macrophylla Willd as Tibetan and Qiang medicinal materials,so as to improve its quality standard and evaluate the quality of C.macrophylla Willd in western Sichuan Province.[Methods]C.macrophylla Willd produced from western Sichuan Province was used as the sample,and the contents of moisture,total ash,acid-insoluble ash,extract,total flavonoids and quercetin in the ground part of C.macrophylla Willd were determined in accordance with the methods of Chinese Pharmacopoeia(2020 edition).With the above seven indicators as evaluation indicators,the quality of medicinal materials was comprehensively evaluated by cluster analysis and principal component analysis(PCA).[Results]According to the results of each indicator,the moisture content of C.macrophylla Willd sample should not exceed 11.00%,the total ash content should not exceed 18%,the acid-insoluble ash content should not exceed 6%,the extract content should not be less than 19%,the total flavone content(calculated by quercetin)should not be less than 2%,and the quercetin content should not be less than 0.15%.[Conclusions]The sample S7 has the best quality and S6 has the worst quality.In this study,the quantitative analysis method of total flavonoids(quercetin)and quercetin in C.macrophylla Willd was established,and the limits of each indicator were preliminarily formulated.
文摘The efficiency of 7 treatment methods for breaking dormancy in zoysiagrass ( Zoysia japonica Steud.) seeds was synchronously compared. Two methods in which seeds soaking in 30% NaOH solution for 20 or 80 minutes were the optimum for breaking dormancy of seeds due to their simple and easy operation in techniques, economic and rapid effectiveness in performance and high germination rates (77.6% and 88.0% respectively).
基金Supported by National Natural Science Foundation of China(31301372)Key Project of Science and Technology Plan of Zhejiang Province(2011C12030)Innovation Training Project of Zhejiang Agriculture and Forestry University(201301004)~~
文摘To rapidly obtain high-quality genomic DNA from Chenopodium quinoa Willd, the genomic DAN in different tissues （leaves, stems and roots） of Chenopodi- um quinoa Willd was extracted by modified CTAB method, SDS method and high- salt Iow-pH method, respectively. The quality and yield of extracted DNA was deter- mined using agarose gel electrophoresis and UV spectrophotometry. At the same time, the PCR-SSR and SSCP molecular detection was also performed. The results showed that the gel test strips, without obvious decomposition, of all the extraction methods were relatively obvious; the genomic DNA yield extracted by modified CTAB method was highest, followed by that by SDS method, and the genomic DNA extracted by high-salt Iow-pH method was lowest： the genomic DNA yields extracted by different methods from Chenopodium quinoa Wiltd leaves were all high- er than those from roots and stems; the quality of Chenopodium quinoa Willd ge- nomic DNA extracted by modified CTAB method and high-salt Iow-pH method was better, and polyphenols, polysaccharides and other impurities were removed more completely. The PCR-SSR and SSCP detection results showed that the genomic DNA extracted by different methods from different tissues of Chenopodium quinoa Willd all could be better amplified, and high-quality strips could be obtained. So the Chenopodium quinoa Willd genomic DNA extracted by the three methods all can be used for subsequent molecular biology research.
基金This study was supported by the NKBRSF (G1999043407-1) Knowledge Innovation Program of the Chinese Academy of Sciences (KZCX2-406+3 种基金 SCXZD0101) NKTRDP (2001BA510B-07 2002BA516A20) and Education Committee Projects of Liaong Province (990121400
文摘The age-structure of natural population of Zoysia japonica in Xiuyan County of Liaoning Province was studied by generational method. The results showed that the highest tiller age class was three, but 1st age class tillers held dominant posi-tion with proportions over 95% in each month during the growing seasons. The 2nd age class and 3rd age class tillers were minority in the population. So Z. japonica population was an expanding population. The zero age class buds on the rhizomes were dominant in buds age structures. The proportion of buds to tillers on quantity in each month was about 30% to 40% and reached the highest at the end of September. The increasing of buds proportion before dormancy guaranteed the quantity of tillers in the next spring. The biomass of 1st age class tillers changed with time. The biomass kept increasing from April to July and reached the highest at the end of July and then decreased.
基金Supported by National Natural Sciences Foundation of China(31301372)the Major Science and Technology Project of Zhejiang Province(2011C12030)the Haixi Prefecture science and technology project of Qinghai Province(2012-Y01)~~
文摘Callus induction effects of nine varieties of Chenopodium quinoa Willd. were compared by taking stem segments and cotyledons of C. quinoa as the ex- plants. At the same time, callus JnductJon of stem segments was optimized, as well as the callus proliferation system. Research results showed that the optimal explant for callus induction was stem segment. The average callus induction rate of nine varieties reached 90% in culture medium MS ＋ 0.5 mg/L 2, 4-D. In the callus opti- mization test, treatment VI （MS ＋ 0.5 mg/L 2, 4-D ＋ 0.5 mg/L KT ＋ 0.5 mg/L NAA） and treatment II （MS ＋ 0.5 mg/L 2, 4-D） had close induction rate, but the callus morphology was greatly different. The latter had loose, glossy and yellowish white calluses. Therefore, culture medium MS ＋ 0.5 mg/L 2, 4-D was the optimal for callus induction. And using 2, 4-D together with KT and NAA could significantly increase the proliferation rate of calluses.
基金the National Nature Science Foundation of China (No.30772890)the Collaborative Education,Research and Development Project of Beijing Municipal Commission of Education and the Key Project of Chinese Ministry of Education (No.108132).
文摘A new tdterpenoid glycoside, 3-β-O-β-D-glucopyranosyl presenegenin 28-O-α-L-arabipyranosyl（1 → 3）-6-β- xylopyranosyl（1 → 4）-[β-D-apiofuranosyl（1 → 3）]-Ct-L-rhamnopyranosyl（1 → 2）-[α-L-rhamnopyranosyl（1 → 3）]-β-D-fucopyranosyl ester （1） was isolated from the Polygala tenuifolia Willd., together with two known saponins, including polygalasaponinXXIV （2） and polygalasaponinXXVIII （3）. The structure of new compound was elucidated by spectroscopic methods.
基金Supported by the National Natural Science Foundation of China（Nos.20176049 and 20576113）
文摘Ultrasonically assisted extraction of isoflavones from the stem of Pueraria lobata （Willd.） Ohwi has been carried out with an ultrasonic extracting apparatus （20kHz, electrical power input to the transducer in 0-650W）. The influence of the electrical power input and extraction time on the＇extraction yield is investigated in water, n-butanol, and 95% （by volume） and 50% （by volume） ethanol aqueous solution. The experimental results indicate that the yields of total isoflavones are higher in ultrasonically assisted extraction than those obtained from con-ventional extraction.Moreover,a mathematical model is proposed,by introducing the electrical power input to index the ultrsound intensity,to describe the behavior of ultrasonically assisted extraction.It is found that the model calcuations are in good agreement with the experimental data.
