Objective To explore the effect of Chinese herbs on PRLR, PR, ER mRNA of decidue in Bromocriptine-induced hypoprolactin abortion rat model from gene transcription level, and observe the changes of blood PRL, P, E2.Met...Objective To explore the effect of Chinese herbs on PRLR, PR, ER mRNA of decidue in Bromocriptine-induced hypoprolactin abortion rat model from gene transcription level, and observe the changes of blood PRL, P, E2.Methods RT-PCR method was taken to analyses the differences of PRLR, PR, ER mRNA in decidue between model group (A group) and model + herbs group (A + H group); RIA was taken to measure the serum levels of PRL, P, E2. Results PRLR, PR mRNA expression in decidue of Group A was significantly lower than the A + H group (P<0. 01) , no difference in ER mRNA expression (P>0. 05); the abortion rate of Group A was 67 %, Group A+H was 17% , the difference was significant; as for the PRL and P level of day 7-1.0, the A group was significantly lower than the A+H group (P<0. 05).Conclusion Bromocriptine could induce abortion by declining the blood PRL, P level and downregulating PRLR, PR mRNA expression in decidue. Chinese herbs might maintain pregnancy by promoting PRL, P secretion and upregulating PRLR, PR mRNA expression in decidue.展开更多
基金This study was sponsored by Shanghai Sci-Tech Dvelopment Fund.No.981
文摘Objective To explore the effect of Chinese herbs on PRLR, PR, ER mRNA of decidue in Bromocriptine-induced hypoprolactin abortion rat model from gene transcription level, and observe the changes of blood PRL, P, E2.Methods RT-PCR method was taken to analyses the differences of PRLR, PR, ER mRNA in decidue between model group (A group) and model + herbs group (A + H group); RIA was taken to measure the serum levels of PRL, P, E2. Results PRLR, PR mRNA expression in decidue of Group A was significantly lower than the A + H group (P<0. 01) , no difference in ER mRNA expression (P>0. 05); the abortion rate of Group A was 67 %, Group A+H was 17% , the difference was significant; as for the PRL and P level of day 7-1.0, the A group was significantly lower than the A+H group (P<0. 05).Conclusion Bromocriptine could induce abortion by declining the blood PRL, P level and downregulating PRLR, PR mRNA expression in decidue. Chinese herbs might maintain pregnancy by promoting PRL, P secretion and upregulating PRLR, PR mRNA expression in decidue.
