Structural Elucidation of the Polymeric Condensed Tannins of Acacia nilotica Subspecies by ^(13)C NMR, MALDI-TOF and TMA as Sources of Bioadhesives

Tannin was extracted from different subspecies of Acacia nilotica,Acacia nilotica nilotica(Ann),Acacia nilotica tomentosa(Ant)and Acacia nilotica adansonii(Ana).The aim was to elucidate their structure and evaluate their reactivity as bioadhesives in the wood industry.The extracts were prepared by hot water extraction(90°C tem-perature).Their gel time with paraformaldehyde was used atfirst to compare their reactivity.The tannin contents and the percentage of total polyphenolic materials in different solutions of the extracts spray dried powder were determined by the hide powder method.Concentrated solutions(47%)were tested by both MALDI ToF,13CNMR.The thermomechanical analysis(TMA)was performed to evaluate their modulus of elasticity(MOE)at different pHs.The gel times of all the three tannin extracts showed that their reactivity and it was com-parable to other known procyanidin/prodelphinidin tannin extract types.Ana,Ann and Ant showed highest per-cent of total polyphenolic materials at 70%,64%,and 57%,respectively.The 13CNMR spectra showed that the three subspecies of condensed tannins were mainly constituted of procyanidins(PC)and prodelphinidins(PD)in slightly different ratios.Ann(56.5%PC and 43.4%PD),Ant(57%PC and 43%PD)and Ana(58%PC and 42%PD).MALDI–TOF spectra showed the presence offlavonoid monomers,and oligomers some of which linked to short carbohydrates monomers or dimers.TMA revealed that the three types of tannins had high MOE at their initial pH(5).

Tannins from Acacia mangium Bark as Natural Dyes for Textiles:Characteristics and Applications

Tannins are capable of producing natural dyes with antioxidant and antibacterial propertis,while synthetic dyes are commonly used in the textile industry,causing environmental issues like water pollution.This research aims to utilize waste tannins as natural dyes as an alternative to synthetic dyes.This study examined the effect of the extraction method on tannin properties such as phenolic content,antioxidants,and antibacterial activity.In addition,Pyrolysis Gas Chromatography‒Mass Spectrometry(Py-GCMS)analysis was used to identify the effect of extraction temperature on the chemical elucidation of tannin.The effect of tannin concentration was evaluated against four bacteria that are usually found on human skin:Staphylococcus epidermidis,Bacillus subtilis,Propionibacterium acnes,and Staphylococcus aureus.Extraction temperature significantly influences the chemical composition of tannin,which leads to different antioxidant properties.The maximum antibacterial properties of tannin were obtained at 90℃with the inhibition zone in the range of 0.9–1.0 mm against four bacteria,tannin yield of 26.59%,Gallic Acid Equivalents or total phenolic content(GAE)of 40.30 mg/g,and Radical Scavenging Activity or antioxidant activity(RSA)of 89.88%.Moreover,the concentration of tannin was significantly linear with its antibacterial properties.Tannin was successfully applied to the textile by using alum as a mordanting agent to create an antibacterial textile.The textile’s bacterial structure damage was analyzed under Field Emission Scanning Electron Microscopy(FESEM).After 50 washings,tannin-textiles with alum-modified properties remained stable compared to those without alum,with S.aureus and S.epidermidis being the most vulnerable bacteria,as confirmed by FESEM images.Hence,tannin is a feasible alternative to harmful and nondegradable synthetic dyes and antibacterial agents.

Antioxidant Activity and Phytochemical Screening in Acacia rigidula Benth. Leaves

This study aimed to compare the total phenolic content (TPC) and antioxidant activities of A. rigidula extracts. It also aimed to identify phenolic acids present in the extracts. The 2,2-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), 2,2-diphenyl-1-picrylhydrazyl (DPPH), ferric reducing antioxidant power (FRAP), and ferric thiocyanate lipid peroxidation antioxidant assays were performed. High performance liquid chromatography was used to identify phenolic acids. There was no solvent effect on TPC nor on scavenging activities, and inhibition of lipid peroxidation (p > 0.05) among solvent extracts. On the other hand, 1:1:3 water: acetone: methanol extract (10.22 mg GAE/g sample) had significantly higher reducing potential than 50% ethanol extract (EE) (9.259 mg GAE/g sample) (p < 0.05);but EE was not significantly different from 80% methanol extract (9.781 mg GAE/g sample) (p > 0.05). Phenolic fraction designated as fraction 4 had the highest antioxidant activity (p < 0.05) with 69.49% ABTS scavenging activity and FRAP reducing potential, 22.26 mg of GAE/g sample. DPPH scavenging activities of fractions 4 (55.59%) and 5 (55.64%) were significantly higher than the other fractions (p A. rigidula extracts contain gallic, caffeic, vanillic, p-coumaric, salicylic acids and vanillin.

Antimicrobial activities of Acacia genus:A review

More than 1 300 species of the vast genus Acacia are found in tropical habitats. They are crucial economic plants since they produce traditional medicines, timber, and gum. The pharmacological uses of the Acacia genus include anti-diarrheal, anti-malarial, chronic pain relief, wound healing, anti-cancer, anti-rheumatism, and antidiabetes activities. It is also used for treating various illnesses such as gastroenteritis, allergies, Alzheimer’s disease, cough, and cardiovascular disease. The present review aims to summarize the antimicrobial activities including the antibacterial and antifungal activity of the Acacia genus. The literature was searched in books and online databases including SciFinder, Google Scholar, Scopus, Pub Med, and scientific journals using the most relevant keywords: Acacia+antimicrobial, Acacia+antibacterial, and Acacia+antifungal.

Effect of Acacia albida Leaf Supplementation on Reproductive Parameters and Pre-Weaning Growth of Arabian Lambs

A trial on Acacia albida leaf supplementation on reproductive parameters and pre-weaning growth of Arabian lambs was conducted from September 2020 to May 2021 at the small ruminant station of the Livestock Research Institute for Development (IRED) in N’Djamena, Chad. Forty-eight (48) Arabian sheep (45 non-pregnant females and 3 males for a sex ratio of 1 ram to 15 ewes) with an average weight of 20.3 ± 1.2 kg and aged approximately 2 years were divided into 3 groups of 15 ewes each. The supplemented group received in addition to the dominant forage of the pasture (300 g of Dactyloctenium aegyptium + 300 g of Cenchrus ciliaris + 600 g of Panicum maximum), 400 g and 600 g of Acacia albida leaves/animal/day while the control group received only 300 g of Dactyloctenium aegyptium + 300 g of Cenchrus ciliaris + 600 g of Panicum maximum. From the results of this study, it appears that the ewes that received 400 g of Acacia albida leaves had a significantly higher fertility rate and birth mortality rate (93.03% and 6.25% respectively). Animals in the control group had a significantly higher pre-weaning mortality rate than those receiving Acacia albida leaves as a supplement (16.66% versus 6.66% and 14.28% respectively). The highest weight of the lambs was observed in the ration supplemented with 600 g of Acacia albida leaves (12209.66 g). The total weight gain, as well as the average daily weight gain of the lambs receiving the 600 g Acacia albida leaf ration was significantly higher than those of the other rations. Supplementation with 400 g and 600 g of Acacia albida leaves can be recommended as a protein supplement for breeding ewes and pre-weaned lambs.

Determinants of Smallholder Farmers Adoption of Acacia decurrens in Northwest Ethiopia

Planting a fast-growing multipurpose Acacia decurrens (AD) tree is one of the climate-smart agricultural practices that have been promoted in Ethiopia, which is widely practiced and an important livelihood strategy in Awi zone. However, the extent of its adoption varies considerably among households in the study area. This study investigated the determinants of intensity of adoption of AD among 385 randomly selected rural households in Awi Zone. Data were gathered using a cross-sectional household survey. Descriptive statistics and Two-limit Tobit model were employed for data analysis. The result of the study shows that on average the intensity of adoption of AD was 0.43 (43% of the total cropland area), though majority of the farmers (48.8%) belong to low level of adoption. The result of the analysis shows that being male, educational level, access to seedling, experience in growing the tree, extension contact has positive and significant relationship with the intensity of adoption. Age of head of household, land holding size, livestock holding size, soil fertility status, disease emergence and road distance have negative and significant influence on intensity of adoption. These suggest that expanding road infrastructure, education, access to seedling, secure land property right, disease management, and provision of extension services related to the AD can also improve smallholder farmers’ intensity of AD adoption. The generated information provides a picture of the study area’s situation to the attention of policy makers, development practitioners and institutional service providers to formulate a better policy intervention to sustain smallholder farmers’ AD plantation.

Survival of Seedlings of Native Woody Species in the Rehabilitation of Gold Mined-Site within a Three Years Old Acacia mangium Willd Plantation of St. Elizabeth-Mahdia, Guyana

Active restoration is a critical component of biodiversity conservation for degraded tropical forest ecosystems caused by artisanal gold mining, and the success of restoration is dependent on native species selection. However, significant knowledge gaps exist regarding when and where to plant trees. This article reports on a revegetation trial undertaken in St Elizabeth, Mahdia, Guyana, to assess the survival and RGR (Relative Growth Rate) of three native woody trees and shrubs planted within three years old Acacia mangium Willd trees pruned and unpruned blocks. ANOVA (Analysis of Variance) for a completely randomized block design with four blocks, two pruned and two unpruned, within A. mangium plots. Biochar treatment was added to the plants during transplanting. Thirty-six (36) wildlings of Humiria balsamifera (Aublet.) (Tauroniro), Goupia glabra Aublet (Kabukalli), and Vismia guianensis (Aublet.) Choisy (Bloodwood) were collected and raised in a tree nursery for two weeks. The native plants were transplanted 3 m apart, survival observations and each seedling’s initial height and diameter were measured and recorded. After the experiment, 13% of seedlings from a population of 720 had died, with the highest mortality being experienced at the 92 days of the experiment (t ≤ 122 days). While the overall survival rates were high, emphasizing the importance of field trials on native and exotic species in different environments is essential to fill the knowledge gaps on suitable species for restoration in degraded areas with other land use histories.

DNA polymorphisms and genetic relationship among populations of Acacia leucophloea using RAPD markers

RAPD （randomly amplified polymorphic DNA） markers were employed to characterize polymorphisms among 5 provenances of Acacia leucophloea and to detect genetic relatedness of the species with 6 other acacias （A. holosericea, A. auriculiformis, A. mangium, A. dealbata, A. ferruginea, and A. nilotica） widely grown in India. Of 194 markers scored for the provenances, 29.38% exhibited polymorphism. Also, 326 markers were generated among 7 species of Acacia, accounting for 55.82% of the polymorphisms. The fifteen 10-mer primers employed were capable of producing 1-8 polymorphic bands for the provenances, and 6-17 for all seven species of Acacia. The genetic similarity coefficient based on Jaccard＇ s coefficient revealed that provenances Thirumangalam and Dharmapuri were closely related. The dendrogram based on a sequential agglomerative hierarchical non-overlapping （SAHN） clustering analysis grouped 4 provenances of A. leucophloea （Dharapuram, Thirumangalam, Pudukottai and Dharmapuri） into one cluster and the other provenance, Sendurai, into a separate cluster. The genetic similarity matrix for 7 Acacia species showed that A. nilotica and A. dealbata were distantly related, while A. holosericea and A. ferruginea were very closely related. Cluster analysis grouped the species of Acacias into 3 major groups of which A. dealbata alone formed a separate group. The RAPD markers generated 36 provenance-specific markers and 162 species-specific markers that could have strong applications for species identification and tree breeding programs for A. leucophloea and for other Acacia species included in this study.

Cold tolerance of Acacia karroo and A.nilotica,two tree species from South Africa,in Beijing

Fynbos plant communities developed under geographic and climatic conditions unique to South Africa. Acacia karroo is the most widespread tree species, well known for its drought tolerance. A. nilotica exudes a good quality gum which is edible and suitable for confectionery. The two species have been introduced to China but little is known about their adaptability under the new environmental conditions. For our present study, the growth potential and physiological and biochemical parameters of the two spe- cies in response to cold stress were investigated. The results, measured as seed qualities （purity, thousand seed weights, germination, etc.） show differences between A. karroo and A. nilotica. The seed germination rate was between 10% and 60%. Seedlings were es- tablished in fields, pots and plugs. Those in the field grew well during the growing season but completely died in the winter. Those in pots or plugs were moved to and managed in the greenhouse. The plants were green in winter. The electrical conductivity of cells, the contents of water soluble sugars, proteins, malondialdehyde （MDA） increased during eight hours of low temperature treatment at 4℃ The activities of superoxide dismutase （SOD）, peroxidase （POD） and catalase （CAT） decreased markedly during the eight hours of 4℃ treatment. We conclude that A. karroo and A. nilotica have little adaptability to the cold winter weather and cannot survive in the outdoors in the Beijing area, but could grow well indoors as ornamentals.

马占相思 (Acacia mangium)与湿地松 (Pinus elliotii)人工林枯落物层的水文生态功能

分析了马占相思与湿地松人工林枯落物的蓄积量、年凋落量及凋落动态、枯落物层对大气降水的截留、以及枯落物抑制土壤水分蒸发和阻滞径流的效应。结果表明 :1 1 5龄的马占相思林枯落物蓄积量 32 .3t/hm2 ,年凋落量 1 1 .1 4 t/hm2 ,最大持水率 2 53.7% ,最大持水量 2 8.2 6t/hm2 ;1 5龄的湿地松林枯落物蓄积量 1 8.7t/hm2 ,年凋落量 7.30 t/hm2 ,最大持水率 2 1 6.7% ,最大持水量 1 5.82 hm2 ;2 2种林分对大气降水的截留率分别为 1 5.9%和 1 1 .7% ,截留率随 1次降水降水量 ( >1 0 mm)的增加而减少 ;3 2～ 4 cm枯落物覆盖下不同含水量的土壤水分蒸发比无覆盖的土壤减少 1 8.2 %～ 78.3% ,枯落物层减少土壤水分蒸发的效应随枯落物层厚度和土壤含水量的增大而增加 ;4 2种枯落物对径流流出时间的阻滞效应随径流深 ( <3mm)和坡度的增加而减小 ,随枯落物层厚度的增加呈直线增加。通过与部分其它森林类型枯落物层水文生态功能比较 。

马占相思(Acacia mangium)树干液流密度和整树蒸腾的个体差异

利用Granier热消散探针观测了华南丘陵地区马占相思人工林（18a树龄）的树干液流（Sap Flow）。日变化的观测结果显示，不同大小胸径的14株样树液流密度（山）个体间的差别较大（CV：36．42％-80．80％），日间最大液流密度从最高的80．05（gH2Om^-2s^-1）到最低的11．25（gH2Om^-2s^-1），差异显著。液流密度的个体差异与树形的大小并不显著相关（P〉0．24），即液流密度的大小不是与树木形态相关联的固有特征。然而，树木胸径的大小却是影响液流随时间变化的重要因子。树形较大的树木日总液流量（E1，kgH2Otree^-1d^-1）较高，但中等大小的树木却具有较高的单位基面积日液流量（E2，kgH2Odm^-2d^-1），发现，单位基面积的日流量最大值并不出现在胸径最大的树木，而出现在胸径稍小的树木，意味着后者对自身结构的水分利用效率较高。由液流密度计算的整树蒸腾，个体间差异也比较大（3．35—72．42kgH2Otree^-1d^-1），且与胸径以幂函数的形式呈现正相关（P〈0．001）。尽管整树蒸腾在个体之间的差别较大，但随时间的变化规律却是一致的，整树蒸腾的变化格型基本上受环境因子的控制。以液流反映马占相思的蒸腾与冠层的实际蒸腾存在明显的时滞，14株样树的液流变化比光合有效辐射滞后40,in至110,in，相关分析显示，时滞的长短与树木的胸径、高度、边材面积和冠幅均不呈现明显的相关性（P〉0.36）。

不同年龄阶段马占相思(Acacia mangium)人工林营养元素的生物循环

对马占相思人工林6种营养元素(N、P、K、Ca、Mg、S)的含量、积累、分布和生物循环特点以及随林分年龄的变化趋势进行了研究。结果表明:(1)林木不同组分营养元素含量的大小次序为树叶>干皮>活枝>枯枝或树根>干材;各组分和凋落物中营养元素含量以N最高,其次是Ca或K,然后是S和Mg,P最低;林地土壤中,以K的含量最高,其次是Ca、Mg、N和P,S最低;随林龄的增加,0～40cm土壤N、P和S含量呈增加趋势;(2)4年、7年生和11年生林分营养元素总积累量分别为1022.08、1997.08和2633.45kg.hm-2,其中乔木层营养元素贮存量依次占73.64%、82.39%和83.65%,林下植被层依次占13.74%、8.74%和6.20%,地表现存凋落物层依次占12.62%、8.87%和10.16%;乔木层以N积累量最大,占总贮存量的53.90%～60.07%,P最小,仅占0.90%～1.23%;(3)马占相思林中不同组分营养元素积累量的分配随林龄的增长发生变化,由4年生以树叶和树枝占主导,逐渐转移到7年生和11年生以干材和树皮为主导;(4)林分营养元素年积累量依次为7年生(235.06kg.hm-.2a-1)>11年生(200.26kg.hm-.2a-1)>4年生(188.16kg.hm-.2a-1);林木各组分营养元素年积累量总的变化趋势为:树干>树叶>树枝>根系>树皮,同一组分各营养元素年积累量与各组分营养元素积累量变化顺序一致,即为N>Ca>K>S>M>P;(5)林分营养元素年吸收量分别为382.35、432.04kg.hm-.2a-1和403.15kg.hm-2.a-1,年归还量分别为194.19、196.98kg.hm-.2a-1和202.89kg.hm-.2a-1,营养元素的循环系数分别为0.51、0.46和0.50,利用系数为0.51、0.26和0.18,周转期为3.88、8.35和10.86。可见,马占相思人工林早期营养元素利用率低,归还速率较快,林分生长到近熟期(11a)时营养元素的周转期较长,但其归还速率仍然较快,有利于林地地力的恢复、维持和提高。

基于树干液流测定值的马占相思(Acacia mangium)冠层气孔导度计算及数值模拟

为深入揭示华南地区马占相思冠层气孔导度对环境因子的响应规律,在2005年7月至11月,利用Granier热消散式探针法对马占相思(Acacia mangium)的树干液流(sapflow)进行了连续测定,计算出整树的蒸腾,并由Penman-Monteith方程得出马占相思的冠层气孔导度值。通过分析,发现:马占相思冠层气孔导度是控制马占相思树整树蒸腾的主要因素;冠层气孔导度随着水汽压亏缺增加呈负指数函数下降的趋势。使用包括了太阳总辐射、水汽压亏缺和气温的Jarvis模型可以较好地模拟马占相思冠层气孔导度对环境因子的响应特征;模拟结果表明:环境变量对模型精确度的影响程度依次为:水汽压亏缺>太阳总辐射>气温。

不同径级马占相思(Acacia mangium)整树蒸腾的湿、干季变化

利用Granier树干液流测定系统对广东鹤山丘陵地马占相思（Acacia mangium）林进行长期监测,并同步监测环境因子（空气温度、相对湿度、光合有效辐射、土壤体积含水量）,选择胸径具有代表性的样树,结合马占相思的形态学参数,计算马占相思的整树蒸腾。通过对光合有效辐射（PAR）分级,建立不同辐射强度等级的湿季（土壤水分θ≥33%）整树蒸腾与水汽压亏缺（VPD）的相关方程,以干季（θ≤24%）的VPD代入对应PAR等级的湿季的拟合方程,求出干季的潜在蒸腾,以潜在蒸腾和干季实际蒸腾之差分析不同径级整树蒸腾在不同季节的实际变化。在所有的PAR分级内,干季整树蒸腾显著低于湿季,仅占湿季蒸腾的10%-20%,就蒸腾减少的绝对量而言,优势木〉中间木〉劣势木。土壤水分下降缩小了不同径级树木之间液流密度的差异,土壤水分亏缺限制了树木的蒸腾,对马占相思的生长造成一定程度的水分胁迫。

厚荚相思(Acacia crassicarpa)腋芽组培快繁技术体系研究

以16年生厚荚相思优树新生带腋芽茎段为外植体进行组织培养和快繁体系建立的研究,结果表明:含腋芽茎段经75%酒精和0.1%升汞分别处理30S和12min后,接入MS+蔗糖2%上进行初代培养,存活率为67.33%,芽诱导率为80.56%;低浓度的活性炭可有效缓解组培苗玻璃化现象,最佳增殖培养基为改良MS+6-BA0.5 mg·L-1+NAA 0.25 mg·L-1+Ac 0.1 g·L-1+蔗糖3%,35 d增殖倍数可达3.72;最佳生根培养基为改良MS+IBA 0.5 mg·L-1+蔗糖2%,生根率为98.83%;将生根苗移栽至以黄心土为基质的营养杯中,存活率为90.00%。

Effect of different pre-sowing treatments on seed germination percentage and growth performance of Acacia auriculiformis

Seed morphology,germination and seedlings growth of Acacia auriculiformis were studied.The experiment was conducted in the nursery of Forestry and Wood Technology Discipline,Khulna University,Bangladesh.Matured seeds of the species were collected from healthy trees of road side plantation from different areas of Khulna District,Bangladesh and treated with five pre-sowing treatments（control,immersion in cold water,immersion in hot water,scarification with sand paper and immersion in concentrated H2SO4）.The average length,breadth and thickness were found to be（0.58±0.017） cm,（0.44±0.007） cm and（0.20±0.089） cm,respectively.Germination was conducted in polybags with a mixture of top soil,coconut husk,coarse sand,and fine sand in a ratio of 3：4：1：1.Results reveal that pre-sowing treatments influences the germination rates of seeds that significantly increase the percentage germination compared with those in control（43%） and cold water treatment（52%）.The highest germination success rate was found 83% in hot water treatment followed by 78% in scarification with sand paper,and 75% with immersion in H2SO4.Germination started from 7 to 12 days and completed between 28 and 35 days period in all treatments.ANOVA showed the significant difference（p0.05） among the treatments in seed germination,but no significant difference among treatment with regard to starting day,closing day and total germination period.In case of height and diameter growth,seedlings originated from the seeds with hot water treatment shows significantly higher in wet season（from May to July）.Hot water treatment can be recommended on seed germination of the species in rural Bangladesh.

Competition-density effect of tree organs in Acacia auriculiformis stands

The competition-density (C-D) effects for mean mass for tree, stem, branch and leaf were analyzed in Acacia auriculiformis stands. Mean tree mass-density and mean organ mass-density were well explained by the C-D equation of tree and the C-D equation of tree organ, respectively. An equation describing the relationship between mean leaf area u and density was formulated that fit the u-data well. The relationship between mean tree mass w and the ratio of each organ to mean tree mass (wo/ w) was examined. With increasing w, the stem mass ratio wS/w increased, whereas the branch mass ratio wB/w and the leaf mass ratio wL/w decreased. The yield difference between the lowest-density stand and the high-density stand became greater with stand growth. However, the yield of the mid-density stand was slightly lower than the yield of the high-density stand during the experimental period. To produce the most desirable combination of demanding individual-tree size and relative high stem yield, the mid-density is recommended as proper planting density for future management of A. auriculiformis stands.

Gastroprotective effect of Acacia nilotica young seedless pod extract:Role of polyphenolic constituents

Objective:To systematically evaluate antiulcer potential of Acacia nilotica in different ulcer models in rats.Methods:Different extracts[ethanolie,50%hydroethanolic(50:50),70% hydroethanolic(70:30) and aqueous]of young seedless pods were examined in pylorus ligation induced gastric ulcers in rats.Various parameters like,volume of gastric acid secretion,pH,free acidity,total acidity,ulcer index,mucin content and antioxidant studies were determined and were compared between extract treated,standard and vehicle control following ulcer induction. The most active extract was also evaluated in swimming stress induced and NSAID induced gastric ulceration.Results:Among different extracts of young seedless pods only hydroethanolic extracts showed significant antiulcer activity in pyloric ligation induced ulceration.Even more the 70%hydroethanolic extract showed better protection as compared to 50%hydroethanolic extract.Further 70%hydroethanolic extract also showed significant mucoprotection in swimming stress induced and nonsteroidal antiinflammatory drugs induced gastric ulceration.Conclusions: The results of present study concluded that the hydroethanolic extract of young seedless pods of Acacia nilotica has antiulcer activity in pylorus ligation,swimming stress and NSAID induced rat ulcer models.The extract containing more amount of phenolic components show high antiulcer activity,indicating the phenolic component of the extract to be responsible for the activity of the extracts.

Stability and solubility improvement of Sompoi(Acacia concinna Linn.) pod extract by topical microemulsion

The aim of this study was to enhance the solubility and stability of Acacia concinna extract by loading in a microemulsion for topical application. Both physical appearance and biological activities of the extract-loaded microemulsion were determined in comparison with the extract solution. Pseudoternary phase diagrams of three oil types including tea seed oil, grape seed oil, and sesame oil, together with polysorbate 85 or the mixture of polysorbate 85 and sorbitan oleate as surfactants, and absolute ethanol as a co-surfactant were constructed to optimize the microemulsion area. The selected microemulsion was then characterized for droplet size, polydispersity index, and viscosity. Tea seed oil exhibited the highest microemulsion area in the phase diagram because it had the highest unsaturated fatty acid content. The microemulsion composed of tea seed oil(5%), polysorbate 85(40%), ethanol(20%), and water(35%) exhibited Newtonian flow behavior with the droplet size and polydispersity index of 68.03 ± 1.09 nm and 0.44 ± 0.04, respectively. After 4% w/w of the extract was incorporated into the microemulsion, larger droplets size was observed(239.77 ± 12.69 nm)with a lower polydispersity index(0.37 ± 0.02). After storage in various conditions, both physical appearances and the stability of biological activity of the extract-loaded microemulsion were improved compared to the solution. Therefore, the A. concinna loaded microemulsion may be a promising carrier for further development into a topical formulation and clinical trials for pharmaceutical and cosmeceutical applications are also suggested.

Role of phenolics as antioxidants,biomolecule protectors and as anti-diabetic factors-Evaluation on bark and empty pods of Acacia auriculiformis

Objective:To search for an efficient and inexpensive source of phytoconstituents with antioxidant potential and health promoting traits from bark and empty pods of Acacia auriculiformis （A.auriculiformis）.Methods:Samples of bark and empty pod extracts were analyzed for bioactives（phenolics,flavonoids and proanthocyanidins） and subjected to free radical scavenging activity on DPPH*,ABTS?,OH?,O2*,and NO along with the determination of reducing power, iron chelating activity and peroxidation inhibition.Defensive action of extracts on biomolecules and cell membranes were evaluated by DNA nicking assay and haemolysis inhibition assay respectively,α-amylase andα-glucosidase inhibitory potentials were also determined. Results:All the bioactives analyzed were higher in bark（B） than empty pods（EP）[TPC:B （574.51±16.11）;EP（96.80±3.45） mg GAE/g.TFC:B（94.71±7.65）;EP（24（?）87±20.45） mg RE/g. Proanthocyanidins:B（2.81±0.31）;EP（1.25±0.01） mg LE/100 g DM]except flavonoids.Both the extracts showed higher quenching capacity on DPPH and ABTS（DPPH:B（0.21±0.01）;EP（1.51±0.17） g extract/g DPPH.ABTS:B（111 519.14±79 340.91）;EP（80 232.55±32 894.12） mmol TE/g） with the FRAP of B（84 515.63±3 350.69） and EP（47 940.79±1 257.60） mmol Fe（（?））/g.Iron chelation was not observed.In addition,they showed lower quenching activity on OH?（B（48.95±1.72）;EP（34.94±1.62）%） and equivalent quenching on O2?（B（53.47±3.92）;EP（24.41±2.61）%）,NO（B（49.04±5.04）; EP（51.00±5.13）%）,peroxidation inhibition（B（67.50±5.50）;EP（55.l±2.3）%） and antihaemolytic potential（B（87.60±6.84）%） towards authentic antioxidant standards.Interestingly,Empty pod extracts are devoid of antihaemolytic activity.Both the extracts showed dose dependent DNA protection.Besides this,bark and empty pod extracts exhibited dual inhibiting potential againstα-amylase andα-glucosidase enzymes.Conclusions:On summarization,it insinuated that both bark and empty pods can be used for the preparation of antioxidant/nutraceutical supplements and in anti-diabetic formulations.