Spraying Arginine at 5 Days before Harvest Delays Postharvest Broccoli Senescence via Nutrient Accumulation

To determine the effects of preharvest arginine spraying on the nutritional level of broccoli and the mechanism of action of arginine in improving the storage quality of broccoli,arginine spraying(5 mmol/L)was conducted at 0,1,3,and 5 days before harvest.The appearance,respiration rate,mass-loss rate,electrolyte leakage,glucosinolate,ascorbic acid,total phenol,total flavonoid,total sugar and sucrose contents,and sucrose phosphate synthase(SPS),invertase(INV),sucrose synthase synthesis(SSS)and cleavage(SSC)activities of broccoli samples were observed after 0,2,4,6,8,and 10 days of storage.The results showed that spraying arginine at 5 days preharvest(5-ARG)helped to inhibit broccoli respiration during storage,delay electrolyte leakage,and maintain broccoli color.Furthermore,during the growth stage,total sugar accumulation was higher in the 5-ARG group.In addition,during the storage period,sucrose synthesis was accelerated,while sucrose cleavage was inhibited,resulting in more sucrose retention in postharvest broccoli.In conclusion,5-ARG resulted in the accumulation of more nutrients during the growth process and effectively delayed the quality decline during storage,thereby prolonging the shelf life of broccoli.Therefore,this study provides a theoretical basis for improving postharvest storage characteristics of broccoli through preharvest treatments.

Index Identification of Suitable Temperature at the Booting Stage and Accumulated Temperature over 10℃ during the Whole Growth Period in Rice in the South of China

According to phenological observation data of fourteen stations in Hunan,Hubei and Jiangxi provinces from 1994 to 2006,indices of suitable temperature at the booting stage and accumulated temperature(≥10 ℃)during the whole growth period in early or late rice in the south of China were analyzed by statistics and biological relationship between factors.Indices of the optimum daily mean temperature and the minimum temperature at the booting stage were as follows:Early convention rice variety was 25.7 ℃ and 22.3 ℃ respectively,hybrid variety was 26.5 ℃ and 23.3℃ respectively;while late convention rice variety was 25.6 ℃ and 22.3 ℃ respectively,hybrid variety was 25.8 ℃ and 22.6 ℃ respectively.Indexes of accumulated temperature during the whole growth period were as follows:The early-maturing variety,the middle-maturing variety and the late-maturing variety of early rice was 2 512.1 ℃,2 528.4 ℃ and 2 651.3 ℃ respectively,while the early-maturing variety,the middle-maturing variety and the late-maturing variety of late rice was 3 126.2 ℃,3 031.5 ℃ and 3 335.2 ℃ respectively.The above indexes all met the requirements of identification,which could provide reference for formulating the meteorological standard of rice in the south of China.

Construction of Accumulated Temperature Effect Model for Fertility Alteration of Two-line Sterile Line P88S

[Objective]The research aimed at constructing an accumulated temperature effect model for fertility alteration of two-line hybrid rice P88S.[Method]Analyze the experiment datas of fertility alteration of P88S in Hainan from 2005 to 2006.[Result]The fertilities of P88S with temperatures ranging from 26 to 28 ℃ were determined by the absolute values and the accumulation effects of the temperature.An accumulation effect model of temperatures based on the bistable state was obtained.[Conclusion]The temperature asymmetry of fertility alteration for two-line sterility line in spring and autumn can be elucidated effectively using the accumulated temperature effect model,namely,alternating from fertile to sterile requires higher temperatures,and alternating from sterile to fertile requires lower temperatures.

Accumulated chilling hours during endodormancy impact blooming and fruit shape development in peach(Prunus persica L.)

Winter chill is essential for the growth and development of deciduous species. To understand the relationship between accumulated chilling hours during endodormancy and blooming and fruit shape development, we controlled chilling hours and investigated their effects on blooming date and fruit shape of peaches. The results showed that the number of days to full bloom date and the heat requirement for blooming were negatively correlated with accumulated chilling hours. Accumulated chilling hours were significantly negatively correlated with fruit shape index and fruit tip lengths, suggesting that the number of chilling hours affect the fruit shape development. Fewer accumulated chilling hours may be the major reason for longer fruit shape and protruding fruit tips. In conclusion, our results indicate specifically that decreased winter chilling hours can delay the bloom date and may lead to aberrant fruit shape development in peaches. Our study provides preliminary insights into the response of temperate fruit species to global climate change.

Profile Characteristics and Potential EnvironmentalEffect of Accumulated Phosphorusin Soils ofVegetable Fields in Beliing

Profile characteristics of accumulated P in 10 representativesoils of vegetable fields in suburban districts of Beijing wereinvestigated. Bioavailability of the accumulated P and its potentialeffect n the environment were studied in a greenhouse pot experimentand a soil column experiment. The results showed that theconcentration of Olsen-P in the 0～20 cm soil samples of thevegetable fields ranged from 2.1 to 358.0 mg kg^-1, which was 2 to 10times higher than that of the crop field sin the suburbs of Beijing.Most of the excessive phosphorus was accumulated in the topsoils.

Survival of Pseudomonas fluorescence X16(luxAB)Strain in Soils Accumulated with Mixed Rare Earth Elements

Rare earth elements(REE)are applied as micro-fertilizer in large scale in China and there is growing concern about the environmental effects of REE accumulation in soils. Accumulation of REE was simulated in lab by adding REE to three soils and the survival of Pseudomonas fluorescence X16 strain marked with luxAB gene in soils was detected. Curvilinear regression method was applied to analyze the survival pattern. The stimulation values, EC_(50) and NOEC values for X16 strain were calculated to compare the toxic intensity of REE in different soils. The stimulation(peak)values in red soil, yellow fluovo-aquic soil and yellow cinnamon soil, are 11.55～18.08,(0～2.13), 2.37～4.62 mg·kg^(-1) , respectively. EC_(50) values are 13.47～39.12, 6.59～56.18, 372～1034 (mg·kg^(-1)), respectively.NOEC values are 5.62 ～21.41, 0.00～4.53, 133.3～327.1 mg·kg^(-1), respectively. Tangents values of regression equation of the survival of X16 strain in red soil are the maximum ones indicating that REE accumulation in red soil has stronger inhibitory effects than in other two soils. The soil order, reflecting toxic intensity of REE is as follows: red soil>yellow fluovic-aquic soil>yellow cinnamon soil.

Effective accumulated temperature and developmental threshold temperature for Semanotus bifasciatus(Motschulsky) in Beijing

In order to understand the occurrence and development of Semanotus bifasciatus （Motschulsky）, a borer insect of Platycladus orientalis in parts of northern and eastern China and to improve its prediction, the developmental threshold temperature （C1） and effective accumulated temperature （K） of S. bifasciatus were determined under conditions of constant and variable temperatures. The results show that under constant temperatures the value of C1 has a range of 8.90±1.77℃and the value of K lies between 95.19±13.14 degree-day for eggs; the values for larvae are C1= 13.26±3.06℃ and the number of degree-day is K 2,885.07±187.87 degree-day; for pupae the ranges are C1 = 8.93±2.49℃ and K= 131.20±25.63 degree-day. Under the condition of ambient temperatures, the values for eggs are C1= 17.33±1.24℃ and K= 70.79±8.99 degree-day. It is suggested that S. bifasciatus adults over-winter in a state of dormancy, not at a diapause. Warm winter may accelerate the life cycle ofS. bifasciatus. Equations can be used to predict an early occurrence ofS. bifasciatus.

Spatio-temporal Changes of ≥10℃ Accumulated Temperature in Northeastern China since 1961

The objective of this study was to provide reliable basis for decision making for national food security and layout and structure adjustment of grain production in the northeastern China. The data of mean daily air temperature of 1961-2009 from 106 meteorological stations in the northeastern China were chosen in this study. Using statistical methods and isoline method, the spatio-temporal changes of various decadal ≥10℃accumulated temperature and the climatic means of ≥10℃ accumulated temperature were studied in this paper. The results showed that 1） The geo- graphical distribution of ≥10℃ accumulated temperature in the northeastern China could be influenced directly by the latitude, longitude and altitude. If latitude moved one degree northward, the average decrease amplitude of the climatic means was 101.9℃ in the study area. 2） The means of decadal ≥10℃ accumulated temperature rose since the 1980s, and their increase amplitudes became larger in the 1990s and the 2010s obviously. Compared with those of the 1980s, ≥10℃ accumulated temperature increased by about 100℃ in the mountainous and plain areas in the 1990s; compared with those of the 1990s, ≥10℃ accumulated temperature increased by about 200℃ in the Hulun Buir High Plain and the Songnen Plain, and 100℃ in the Sanjiang Plain and the Liaohe Plain in the 2010s. 3） The means of the decada ≥10℃accumulated temperature for 106 meteorological stations in the northeastern China increased with the rate of 145.57℃/10yr in 1961-2009.4） The climatic means of ≥10℃ accumulated temperature increased from 1961-1990 to 1971-2000 and 1981-2009. Compared with the climatic mean of 1971-2000, that of 1981-2009 had increased by above 50℃ in most of the study area, even up to 156℃. Compared with the climatic mean of 1961-1990, that of 1981-2009 increased by above 100℃ in most parts of the study area, even up to 200℃. 5） The maximum northward shift, eastward and westward extension amplitudes of3100℃, 3300℃ and 3500℃ isolines were larger among all isolines for the climatic means of the three phases. Compared with the positions of the isolines of 1961-1990, those amplitudes of 31000C isoline of 1981-2009 were 145 km, 109 km and 64 km, respectively; those of 3300℃ isoline were 154 km, 54 km and 64 km, respectively; and the maximum northward shift of 3500℃ isoline was about 100 km.

Change Trends of Accumulated Temperature and Effects on Agricultural Production in Shenyang during Recent 58 Years

[Objective] The aim was to study the change trend of accumulated temperature in Shenyang in recent 58 years, as well as its effect on agricultural production. [Method] Based on the surface temperature data in Shenyang, the change trends of ≥0 ℃ and ≥10 ℃ accumulated temperature in Shenyang in recent 58 years were analyzed by means of climatic statistics method, and the effects of accumulated temperature variation on agricultural production were discussed. [Result] In recent 58 years, the first day with temperature ≥0 ℃ advanced 10 d, and the last day put off slightly, while sustained days prolonged 13 d, and ≥0 ℃ accumulated temperature increased by 343 ℃·d; meanwhile, the first day with temperature ≥10 ℃ advanced 9 d, and the last day put off 8 d, while sustained days prolonged 16 d, and ≥10 ℃ accumulated temperature increased by 370 ℃·d; compared with the first 20 years, sustained days with temperature ≥0 ℃ and ≥10 ℃ prolonged 9 d in the last 20 years, and ≥0 ℃ and ≥10 ℃ accumulated temperature increased by 196 and 202 ℃·d, respectively. In addition, the increase of heat resources affected agricultural production in Shenyang. [Conclusion] The study could provide theoretical foundation for grasping heat resources variation and adjusting agriculture distribution.

Analysis on the Changes of Accumulated Temperature,Initial and Terminal Dates and Duration Days of Main Boundary Temperature in Zhangjiakou City

Global warming has become a topic of widespread attention. The climate change will affect the change of agricultural climate resource,thereby affecting the agricultural planting structure,the grain yield,etc. Based on the daily temperature of Zhangjiakou from 1960 to 2011,the temporal changes of ≥0 ℃ and ≥10 ℃ accumulated temperatures were analyzed by using the methods of linear trend estimation and M-K mutation test. The results indicated that(1) the annual average temperature showed an increasing trend with the linear warming rate of 0. 42 ℃ /10 a in the past 52 years. In 1982,the annual average temperature had abrupt change,especially after 1983,the warming trend increased significantly.(2) In the past 52 years,≥0 ℃ accumulated temperature showed an increasing trend with the warming rate of 89. 4 ℃ /10 a. Mutation point of ≥0 ℃ accumulated temperature appeared in 1983,and after 1985,≥0 ℃ accumulated temperature increased sharply. At the same time,the ≥10 ℃ accumulated temperature had abrupt change in 1983,especially from 1987 to 1995 and after 1986,the warming trend increased sharply.(3) The initial date of ≥0 ℃ presented significant advancing trend,and that of ≥10 ℃ had little change,while their terminal dates presented delaying trend,so the sustained days of ≥0 ℃ and ≥10 ℃ increased significantly.(4) In the past 52 years,the delay of the terminal dates of ≥0 ℃ and ≥10 ℃ was greater than the advancing of the initial dates,and ≥0 ℃ accumulated temperature had a greater increase than ≥10 ℃ accumulated temperature.

Improving control effects of absence seizures using single-pulse alternately resetting stimulation (SARS) of corticothalamic circuit

Presently,we develop a simplified corticothalamic(SCT)model and propose a single-pulse alternately resetting stimulation(SARS)with sequentially applying anodic(A,“+”)or cathodic(C,“−”)phase pulses to the thalamic reticular(RE)nuclei,thalamus-cortex(TC)relay nuclei,and cortical excitatory(EX)neurons,respectively.Abatement effects of ACC-SARS of RE,TC,and EX for the 2 Hz-4 Hz spike and wave discharges(SWD)of absence seizures are then concerned.The m∶n on-off ACC-SARS protocol is shown to effectively reduce the SWD with the least current consumption.In particular,when its frequency is out of the 2 Hz-4 Hz SWD dominant rhythm,the desired seizure abatements can be obtained,which can be further improved by our proposed directional steering(DS)stimulation.The dynamical explanations for the SARS induced seizure abatements are lastly given by calculating the averaged mean firing rate(AMFR)of neurons and triggering averaged mean firing rates(TAMFRs)of 2 Hz-4 Hz SWD.

Accumulated Rotation of A Modified Suction Caisson and Soil Deformation Induced by Cyclic Loading

Modified suction caissons(MSCs)acting as offshore wind turbine foundations will generate the accumulated rotation under cyclic loading resulted from waves.The accumulated rotation and the range of soil deformation around the MSC under long-term cyclic wave loading were studied using 3-D numerical simulations.The Morison equation was adopted to calculate the wave loadings.It was found that the MSC accumulated rotation increases linearly with the increase of the logarithm of cyclic number.The normalized expression was proposed to reflect the relationship between the accumulated rotation and cyclic number.The soil deformation range around the MSC increases when increasing the cyclic number and loading amplitude.It can also be concluded that the accumulated rotation increases rapidly with this change of excess pore pressure in the first 4000 cycles.The responses of the MSC to wave and wind loads were also investigated.Results show that the accumulated rotation of the MSC under both wave and wind loadings is larger than that under the wave loading only.

Improved strength and ductility of AZ31B Mg alloy sheets processed by accumulated extrusion bonding with artificial cooling

In this study,accumulated extrusion bonding(AEB)process with application of artificial water cooling was successfully performed to fabricate fined-grains AZ31B Mg alloy sheets at 150℃,200℃ and 250℃.The resultant microstructure and mechanical properties are systematically investigated.It reveals that the processing temperature has an important effect on the microstructural evolution during extrusion.During AEB process at 150℃ and 200℃,{10-12}tensile twinning was activated at early stage of extrusion,and subsequently continuous dynamic recrystallization(CDRX)occurred and dominated the further deformation.However,for the sample extruded at 250℃,hardly any twins can be observed,and new fined dynamic recrystallized grains were found along grain boundaries.Artificial cooling was utilized to reduce the rate of grain growth out of the extrusion die,resulting the grains significantly refined from 11μm to 2.5μm.Local high dislocation density region was also observed in the microstructure of sample processed at 150℃ in artificial cooling condition,and the degree decreased with the processing temperature increase.The results summarized from tensile tests indicated that due to the grain refinement the strength and ductility was significantly enhanced(YS of 186 MPa vs.145 MPa,UTS of 391 MPa vs.336 MPa and FE of 31.5%vs.24.5%compared with the as-received sample).Subsequently,annealing treatment at different temperatures was applied to eliminate the high dislocation density.The sample annealed at 200℃ exhibited the best comprehensive mechanical property with YS of 179 MPa,UTS of 390 MPa and FE of 33.0%.As the annealing temperature increasing,the dislocation density was reduced by static recrystallization(SRX)and grain growth,leading to a decreased strength and ductility.

Online volume rendering of incrementally accumulated LSCEM images for superficial oral cancer detection

Laser scanning confocal endomicroscope(LSCEM)has emerged as an imaging modality which provides noninvasive,in vivo imaging of biological tissue on a microscopic scale.Scientific visualizations for LSCEM datasets captured by current imaging systems require these datasets to be fully acquired and brought to a separate rendering machine.To extend the features and capabilities of this modality,we propose a system which is capable of performing realtime visualization of LSCEM datasets.Using field-programmable gate arrays,our system performs three tasks in parallel:(1)automated control of dataset acquisition;(2)imaging-rendering system synchronization;and(3)realtime volume rendering of dynamic datasets.Through fusion of LSCEM imaging and volume rendering processes,acquired datasets can be visualized in realtime to provide an immediate perception of the image quality and biological conditions of the subject,further assisting in realtime cancer diagnosis.Subsequently,the imaging procedure can be improved for more accurate diagnosis and reduce the need for repeating the process due to unsatisfactory datasets.

Sensitivity of Personal Account Accumulated Value of New Rural Social Pension Insurance to Variation of Uncertainties

The personal account of new rural social pension insurance raises funds in the form of individual premium-paying,collectively pooled subsidy and government subsidy.The personal account accumulated value has a decisive influence on the payment level of personal account pension.The personal account accumulated value has a direct or indirect relationship with the income level of farmers,premium-paying level,the insured period,investment return rate of funds and other factors.Analysing the impact of infinitesimal variation of these factors on accumulated value of personal account pension,is of great significance to improving and consolidating personal account,and achieving sustainable development of new rural social pension insurance.

Strength-ductility balance of AZ31 magnesium alloy via accumulated extrusion bonding combined with two-stage artificial cooling Author links open overlay panel

AZ31 Mg alloy with heterogeneous bimodal grain structure(smaller grain size of 5-20µm and coarser grain size of 100-200µm)was subjected to accumulated extrusion bonding(AEB)at 250℃combined with two-stage artificial cooling in this work,viz.local water cooling and artificial cooling.The microstructure developed consecutively as a result of discontinuous dynamic recrystallization(DDRX)for the AEBed samples.{10-12}tensile twinning also played an important role for the AEB with local water cooling at the initial extrusion stage in the container.Local water cooling could further reduce the DRXed grain size to~2.1µm comparing that without water cooling.And the grain growth rate was reduced by artificial cooling out of extrusion die.Under the combination of two-stage cooling,the fine DRXed grains at sizing band were almost retained with average grain size of~2.3µm after the sheet out of extrusion die,and the unDRXed grains with high residual dislocation density accumulation were also reserved.The tensile tests results indicated that a good strength-ductility balance with a high ultimate tensile strength(319 MPa vs.412 MPa)and fracture elongation(19.9%vs.30.3%)were obtained.The strength enhancement was mainly owing to the grain refinement and local residual plastic strain reserved by the artificial cooling.The excellent ductility originated from fine DRXed microstructure and ED-tilt double peak texture.

Attenuation-type and failure-type curve models on accumulated pore water pressure in saturated normal consolidated clay

Based on dynamic triaxial test results of saturated soft clay, similarities of variations between accumulated pore water pressure and accumulated deformation were analyzed. The Parr＇s equation on accumulated deformation was modified to create an attenuation-type curve model on accumulated pore water pressure in saturated normal consolidation clay. In this model, dynamic strength was introduced and a new parameter called equivalent dynamic stress level was added. Besides, based on comparative analysis on variations between failure-type and attenuatiun-type curves, a failure-type curve model was created on accumulated pore water pressure in saturated normal consolidation clay. Two models can take cycle number, coupling of static and dynamic deviator stress, and consolidation way into consideration. The models are verified by test results. The correlation coefficients are more than 0.98 for optimization of test results based on the two models, and there is good agreement between the optimized and test curves, which shows that the two models are suitable to predict variations of accumulated pore water pressure under different loading cases and consolidation ways. In order to improve prediction accuracy, it is suggested that loading cases and consolidation ways should be consistent with in-situ conditions when dynamic triaxial tests are used to determine the constants in the models.

Characteristics of the dynamic changes in active accumulated temperature in Sichuan,China in the last 51 years against the background of climate change

It is of utmost necessity to understand the dynamics of regional active accumulated temperature(AAT)to cope with the negative impacts of global warming on agroforestry development and food security and to provide a real-time and effective reference basis for regional agroforestry planning.The daily temperature data from 30 meteorological stations in Sichuan Province from 1970 to 2020,and sea surface temperature(SST)index data from the Atlantic Multiphase Oscillation(AMO)and Pacific Decadal Oscillation(PDO)were used for the study.Sichuan Province was divided into the western region(WS)and the eastern region(ES),considering 1000 m above sea level as the boundary.The spatiotemporal characteristics of≥0℃ and≥10℃ active accumulated temperature(AAT0,AAT10)in WS and ES were analyzed comprehensively using 5-day average sliding,empirical orthogonal function(EOF),ensemble empirical mode decomposition(EEMD),and multiple mutation tests.The results show that(1)AAT0 and AAT10 of WS ranged from 3034℃ to 3586℃ and 1971℃ to 2636℃,respectively,while the AAT0 and AAT10 of ES ranged from 5863℃ to 6513℃ and 4847℃ to 5875℃,respectively.The period around 1997 was a significant abrupt change,and the AAT in the province generally increased during the subsequent time period(2)AAT in the study area is mainly driven by the fluctuations of AMO,as reflected by the low-to-high variation of AAT coinciding with the jump of the cold-to-warm phase of AMO.Considering different time scale fluctuations in the past 51 years,the major cycle for both AAT0 and AAT10 in WS is 3.40 a,while the major cycles in ES are 3.64 a and 3.19 a,respectively with a sub-cycle of 7.29 a.AAT fluctuation has an insignificant periodic characteristic of 25.50 a on the interdecadal scale(3)The spatial heterogeneity of AAT in WS is prominent and is mainly reflected by the significantly warm conditions in the south of the WS region and relatively slight warm conditions in the north,as well as by the isolated cooling area in the form of"freezing point",i.e.,Xiaojin county.In contrast,the spatial variability of AAT in ES is more or less consistent,with the warming areas concentrated in the foothills of the western edge of the basin and a slight increase in AAT observed in the central part of the basin.

Comparison on Morphological and Optical Properties of TiO_(2) Thin Films Grown by Single-Pulse and Multi-Pulse Laser Ablation

TiO2 thin films were prepared on glass substrates using the PLD (Pulsed Laser Deposition) technique. In order to carry out the ablation process, a Nd:YAG laser was used emitting in 1064 nm wavelength at 10 Hz repetition rate, set up for operating in both single-pulse and multi-pulse regimes. A comparison of the deposition rate, the optical and morphological properties of the layers obtained from both ablation regimes was made, which showed that the multi-pulsed ablation produced layers with a higher surface quality and better optical properties.

Effects of Rice Yield and Quality Across Accumulated Temperature Zone Planting in Cold Area

Five rice varieties were planted to determine the variation of the yield and quality traits in five different regions in a cold area of China. The results showed that the number of the panicles, the number of grains per panicle and percentage of head-milled rice displayed quadratic curves against the accumulated temperature, and the sterile rate decreased with greater accumulated temperature. However, 1 000-grain weight had no correlation with the accumulated temperature and protein content, amylose content and taste also had no obvious relation with the accumulated temperature. The results from the accumulated temperature differed with rice variety, so the temperature insensitive type variety should be proposed for production.