Dyes are common pollutants in textile wastewaters, and the treatment of the wastewater has now attracted much attention due to its wide application and low biodegradability. In this study, Fe^0/C/Clay ceramics, a kind...Dyes are common pollutants in textile wastewaters, and the treatment of the wastewater has now attracted much attention due to its wide application and low biodegradability. In this study, Fe^0/C/Clay ceramics, a kind of novel micro-electrolysis filler, were sintered and employed in a dynamic micro-electrolysis reactor for synthetic Acid Red 73 （AR73） and Reactive Blue 4 （RB4） wastewater treatment. The effects ofinfluent pH, hydraulic retention time （HRT）, and aeration on the decoloration efficiencies of AR73 and RB4 were studied. The optimum conditions for wastewater treatment were： AR73, influent pH of 4, HRT of 2 h and aeration; RB4, influent pH of 5, HRT of 6 h and aeration. Under the optimum conditions, decoloration efficiency of AR73 and RB4 wastewater was 96% and 83%, respectively. Results of UV-vis spectrum scanning demonstrated that the chromophores were broken. Continuous running tests showed that improvement of micro-electrolysis system with Fe^0/C/Clay ceramics for AR73 and RB4 synthetic wastewater treatment could avoid failure of micro-electrolysis reactor, which indicated great potential for the practical application of the ceramics in the field of actual industrial wastewater treatment.展开更多
分别采用饱和硫酸铵法和辛酸—硫酸铵法纯化兔抗酸性红73(acid red 73,AR73)免疫球蛋白G(IgG)。通过二喹啉甲酸(BCA)法、十二烷基硫酸钠—聚丙烯酰胺凝胶电泳(SDS-PAGE)试验、间接ELISA法分别测定IgG的浓度、纯度及效价。结果显示,饱和...分别采用饱和硫酸铵法和辛酸—硫酸铵法纯化兔抗酸性红73(acid red 73,AR73)免疫球蛋白G(IgG)。通过二喹啉甲酸(BCA)法、十二烷基硫酸钠—聚丙烯酰胺凝胶电泳(SDS-PAGE)试验、间接ELISA法分别测定IgG的浓度、纯度及效价。结果显示,饱和硫酸铵法所提IgG浓度为36.44mg/mL,最高效价达1.6×105;辛酸—硫酸铵法所提IgG浓度为15.79mg/mL,最高效价达3.2×105。提示,辛酸—硫酸铵法IgG纯度优于饱和硫酸铵法。展开更多
Since the azo dyes widely used in textile-dyeing industry are toxic, highly persistent, and ubiquitously distributed in the environment, their treatment efficiencies and fade in ecosystems have received worldwide atte...Since the azo dyes widely used in textile-dyeing industry are toxic, highly persistent, and ubiquitously distributed in the environment, their treatment efficiencies and fade in ecosystems have received worldwide attention. Due to the microbes play important roles in azo-dye degradation, exogenous nutrient addition is used as a promising biostimulation strategy to improve the treatment efficiencies of azo-dye wastewater. However, little is known about the effects of different kinds of exogenous nutrients on the azo-dye wastewater treatment directly. Here, three kinds of common nutrients, glucose, starch and yeast extract, were added to the sequencing batch reactors （SBR） for the treatment of wastewater containing acid red 73 to compare the biostimulation efficiencies by investigating the dye＇s removal efficiencies and the changes of related water qualities. Our results showed that the reactors added yeast extract had highest removal efficiencies and chemical oxygen demand （COD） as well, followed by glucose and starch. The removal rates of acid red 73 and COD were 90.63% and 8713% in the reactors added yeast extract, respectively, while 86.49% and 78.4% in those with glucose and 85.38% and 75.2% in those with starch. This study provided some useful information for the biostimulation strategy of azo-dye wastewater treatment and preliminarily suggested that yeast extract would be the optimal choice.展开更多
[Objectives]To identify stained Schisandrae Chinensis Fructus in Lifei Tablets with carmine and acid red 73 as target substances.[Methods]High performance liquid chromatography(HPLC)and liquid chromatography tandem-ma...[Objectives]To identify stained Schisandrae Chinensis Fructus in Lifei Tablets with carmine and acid red 73 as target substances.[Methods]High performance liquid chromatography(HPLC)and liquid chromatography tandem-mass spectrometry(LC-MS/MS)was used to analyze the 70%methanol extract of the drug.The HPLC was equipped with an Inertstain-C_(18)(250 mm×4.6 mm,5μm)chromatographic column.The mobile phase was acetonitrile-5 mmol/L ammonium acetate solution,gradient elution:volume flow rate of 1.0 mL/min,column temperature of 30℃,detection wavelength of 509 nm.LC-MS/MS was equipped with a Waters-C_(18)(2.1 mm×150 mm,1.7μm)chromatographic column,the mobile phase was methanol-0.1%formic acid solution(containing 5 mmol/L ammonium acetate),gradient elution;the volume flow rate was 0.3 mL/min;ESI ion source,positive ion mode,full scan of primary and secondary mass spectrometry.[Results]Carmine and acid red 73 showed good linear relationship in HPLC and LC-MS/MS,with r greater than 0.995.The sample recovery and RSD values of HPLC and LC-MS/MS met the requirements.The RSD of the results determined by HPLC and LC-MS/MS was not greater than 5.0%.Both methods had good compatibility and could be used for the examination of carmine and acid red 73 in Lifei Tablets.[Conclusions]The method is reliable and reproducible and can be used to identify stained Schisandrae Chinensis Fructus in Lifei Tablets.展开更多
文摘Dyes are common pollutants in textile wastewaters, and the treatment of the wastewater has now attracted much attention due to its wide application and low biodegradability. In this study, Fe^0/C/Clay ceramics, a kind of novel micro-electrolysis filler, were sintered and employed in a dynamic micro-electrolysis reactor for synthetic Acid Red 73 （AR73） and Reactive Blue 4 （RB4） wastewater treatment. The effects ofinfluent pH, hydraulic retention time （HRT）, and aeration on the decoloration efficiencies of AR73 and RB4 were studied. The optimum conditions for wastewater treatment were： AR73, influent pH of 4, HRT of 2 h and aeration; RB4, influent pH of 5, HRT of 6 h and aeration. Under the optimum conditions, decoloration efficiency of AR73 and RB4 wastewater was 96% and 83%, respectively. Results of UV-vis spectrum scanning demonstrated that the chromophores were broken. Continuous running tests showed that improvement of micro-electrolysis system with Fe^0/C/Clay ceramics for AR73 and RB4 synthetic wastewater treatment could avoid failure of micro-electrolysis reactor, which indicated great potential for the practical application of the ceramics in the field of actual industrial wastewater treatment.
文摘分别采用饱和硫酸铵法和辛酸—硫酸铵法纯化兔抗酸性红73(acid red 73,AR73)免疫球蛋白G(IgG)。通过二喹啉甲酸(BCA)法、十二烷基硫酸钠—聚丙烯酰胺凝胶电泳(SDS-PAGE)试验、间接ELISA法分别测定IgG的浓度、纯度及效价。结果显示,饱和硫酸铵法所提IgG浓度为36.44mg/mL,最高效价达1.6×105;辛酸—硫酸铵法所提IgG浓度为15.79mg/mL,最高效价达3.2×105。提示,辛酸—硫酸铵法IgG纯度优于饱和硫酸铵法。
基金supported by the National Natural Science Foundation of China(51308133)Guangdong Provincial National Natural Science Foundation(2014A030308019)+1 种基金Science and Technology Planning Project of Guangdong Province(2015A020215016)the support of Guangdong Institute of Microbiology
文摘Since the azo dyes widely used in textile-dyeing industry are toxic, highly persistent, and ubiquitously distributed in the environment, their treatment efficiencies and fade in ecosystems have received worldwide attention. Due to the microbes play important roles in azo-dye degradation, exogenous nutrient addition is used as a promising biostimulation strategy to improve the treatment efficiencies of azo-dye wastewater. However, little is known about the effects of different kinds of exogenous nutrients on the azo-dye wastewater treatment directly. Here, three kinds of common nutrients, glucose, starch and yeast extract, were added to the sequencing batch reactors （SBR） for the treatment of wastewater containing acid red 73 to compare the biostimulation efficiencies by investigating the dye＇s removal efficiencies and the changes of related water qualities. Our results showed that the reactors added yeast extract had highest removal efficiencies and chemical oxygen demand （COD） as well, followed by glucose and starch. The removal rates of acid red 73 and COD were 90.63% and 8713% in the reactors added yeast extract, respectively, while 86.49% and 78.4% in those with glucose and 85.38% and 75.2% in those with starch. This study provided some useful information for the biostimulation strategy of azo-dye wastewater treatment and preliminarily suggested that yeast extract would be the optimal choice.
基金Supported by Chongqing Pharmaceutical Research Project of Chongqing Drug Administration and Chongqing Science and Technology Bureau(Yu Yao Jian[2021]30)。
文摘[Objectives]To identify stained Schisandrae Chinensis Fructus in Lifei Tablets with carmine and acid red 73 as target substances.[Methods]High performance liquid chromatography(HPLC)and liquid chromatography tandem-mass spectrometry(LC-MS/MS)was used to analyze the 70%methanol extract of the drug.The HPLC was equipped with an Inertstain-C_(18)(250 mm×4.6 mm,5μm)chromatographic column.The mobile phase was acetonitrile-5 mmol/L ammonium acetate solution,gradient elution:volume flow rate of 1.0 mL/min,column temperature of 30℃,detection wavelength of 509 nm.LC-MS/MS was equipped with a Waters-C_(18)(2.1 mm×150 mm,1.7μm)chromatographic column,the mobile phase was methanol-0.1%formic acid solution(containing 5 mmol/L ammonium acetate),gradient elution;the volume flow rate was 0.3 mL/min;ESI ion source,positive ion mode,full scan of primary and secondary mass spectrometry.[Results]Carmine and acid red 73 showed good linear relationship in HPLC and LC-MS/MS,with r greater than 0.995.The sample recovery and RSD values of HPLC and LC-MS/MS met the requirements.The RSD of the results determined by HPLC and LC-MS/MS was not greater than 5.0%.Both methods had good compatibility and could be used for the examination of carmine and acid red 73 in Lifei Tablets.[Conclusions]The method is reliable and reproducible and can be used to identify stained Schisandrae Chinensis Fructus in Lifei Tablets.
