Preparation of lactic acid bacteria compound starter cultures based on pasting properties and its improvement of glutinous rice flour and dough

The effects of 5 lactic acid bacteria(LAB)fermentation on the pasting properties of glutinous rice flour were compared,and suitable fermentation strains were selected based on the changes of viscosity,setback value,and breakdown value to prepare LAB compound starter cultures.The results revealed that Latilactobacillus sakei HSD004 and Lacticaseibacillus rhamnosus HSD005 had apparent advantages in increasing the viscosity and reducing the setback and breakdown values of glutinous rice flour.In particular,the compound starter created using the two abovementioned LAB in the ratio of 3:1 had better performance than that using a single LAB in improving the pasting properties and increasing the water and oil absorption capacity of glutinous rice flour.Moreover,the gelatinization enthalpy of the fermented samples increased significantly.For frozen glutinous rice dough stored for 28 days,the viscoelasticity of frozen dough prepared by compound starter was better than that of control dough,and the freezable water content was lower than that of control dough.These results indicate that compound LAB fermentation is a promising technology in the glutinous rice-based food processing industry,which has significance for its application.

Synthesis and Evaluation of Nicotinoylamino Acid Compounds as Radiosensitizer

Nicotinoylamino acid compounds 4, 5, 9a, 9b, 9c, 10a and 10b were synthesized with nicotinoyl chloride or nicotinoyl azide as acetylating agents of amino acid esters or amino acids. The compounds were tested for their radiosensitizing activity in Leukemia cell line（L1210） and compared with nicotinamide; among them, compounds 9a and 9c showed significant radiosensitizing effects, the sensitizer enhancement ratio（SER） was 1.64 and 1.58, respectively, while nicotinamide did not show good radiosensitizing effect under the same conditions. Compound 9c was alone tested for radiosensitization in LA 795 cell-bearing T-739 mice, or hyperthermia and breathing carbogen（5%CO2＋95%O2） were together tested for radiosensitization. The results showed that radiation-induced growth delay was enhanced by 9c alone or by the combination of hypertheimia and carbogen. The tumor-bearing mice were irradiated locally by total 10 Gy, and the tumors grew to three times that of the original volume in an average of 5.8 d. The mice were given i.p. compound 9c at 1000 mg/kg 60 min before irradiation and treated at 43 ℃ for 30 min after irradiation or treated with breathing carbogen for 5 min before radiation or with hyperthmia（43 ℃） for 30 min after irradiation; the time required for the tumor to grow to three times the orginal volume was in an average of 12.9 and 13 d, respectively.

Crystal Structure and Magnetic Properties of a Dinuclear Terbium Compound Tb_2(μ_2-anthc)_4(anthc)_2(1,10-phen)_2

A dinuclear Tb（Ⅲ） compound, Tb_2（μ_2-anthc）_4（anthc）_2（1,10-phen）_2（1, Hanthc = 9-anthracenecarboxylic acid and 1,10-phen = 1,10-phenanthroline）, was synthesized under solvothermal condition and structurally characterized by single-crystal X-ray diffraction. Compound 1 crystallizes in the triclinic system, space group P1, with a = 12.5061（12）, b = 13.3168（10）, c = 15.0079（12） ？, α = 110.620（7）, β = 102.941（7）, γ = 107.036（7）o, V = 2081.8（3） ？~3, Z = 1, C_（114）H_（70）N_4O_（12）Tb_2, M_r = 2005.58, D_c = 1.600 g/cm^3, μ = 1.759 mm^（-1）, F（000） = 1008, the final R = 0.0294 and w R = 0.0608 for 8900 observed reflections with I 〉 2s（I）. In compound 1, two Tb（Ⅲ） ions are linked by four carboxylate groups of the bridging anthc– ligands to give a dinuclear Tb_2（μ_2-anthc）_4 unit wherein each Tb（III） ion is further capped by a terminal anthc– ligand and a 1,10-phen ligand in a chelating fashion. Detailed direct-current and alternating-current susceptibility measurements showed no slow magnetism relaxation phenomenon was observed for 1.

[Hmim]_3PW_(12)O_(40):A high-efficient and green catalyst for the acetalization of carbonyl compounds

[Hmim]_3PW_(12)O_(40) was developed and used in the acetalization of carbonyl compounds in excellent yields.The ionic liquidheteropoly acid hybrid compound and reaction medium formed temperature-dependent phase-separation system with the ease of product as well as catalyst separation.The catalyst was recycled more than 10 times without any apparent loss of catalytic activity.

Efficiency of Chalcone Compounds Inhibitors for Acid Corrosion of Al and Al-3.5Mg Alloy

Corrosion inhibition of Al and Al-3.5Mg alloy by organic compounds, namely chalcones in hydrochloric acid solutions has been investigated by rapid polarization technique and weight loss method. Polarization measurements show that, the inhibitors act cathodically both in case of Al and Al-3.5Mg alloy. It was found from the weight loss measurements that, the inhibition efficiency depends on the substituent in the chalcone compound. The relative inhibitive efficiency of these compounds has been explained on the basis of structure dependent electron donor properties of the inhibitors and the metal inhibitor interaction on the surface. The inhibition efficiency ranges from 16 to 64% for Al and from 30% to 91% for Al-3.5Mg alloy

Compounding Pattern of REE， Clay and Humic Acid in the Weathering Crust of Granites

An anomalous phase containing clays and humic acid was found while extraction humic acid from samples of weathering crust of REE-bearing granites by using 0.1 mol/L NaOH solution.Studied by chemical separation experiments,column chromatography,ion-exchange chromatography,infrared spectrum.fluorescence spectrum,polarimicroscopic testing and elemental determination by ultraviolet-visible spectrometer and atomic absorption spectrometer.it was proved to be a compound of humic acid and clays which are connected in the form of bridge by heteronuclearpolynuclear complexing ions formed by cations of RE￣(5+),Al￣(3+).Fe￣(3+) etc.The compounding pattern was further proved by the experiment for compounding humic acid,REE and kaolinite.

Synthesis and Determination of Absolute Configuration of a Divergent Polyhydroxy Enyne Compound

Polyhydroxy enyne compound （＋）-（1＇S, 2R, 3S, 5S, 6S）-5,6-dimethoxy-5, 6-dimethyl- 2-（1＇-hydroxylpropyl-2-ne）-3-vinyl-l,4-dioxane has been synthesized from D-（-）-tartaric acid. A new chiral center was established by nucleophilic addition with 87% de. The modified Mosher＇s method was employed to confirm the absolute configuration of 17, which assigned the S-configuration at the new chiral center.

STUDY ON SYNTHESIS,CHARACTERIZATION AND STRUCTURE OF A NEW RARE EARTH COORDINATION COMPOUND WITH BENZOIC ACID

In this paper,a new rare earth benzoate coordination compound,[TbL_3(HL)(H_2O)]_2 (HL=C_7H_6O_2=benzoic acid) is synthesized and characterized by elemental analysis DTA,TG,DTC,and IR spectra.The title compound crystallizes in triclinic,space.group P with a=9.148(1),b=11.460(2), c=13.506(2);α=112.76(1),β=91.83(1),γ=91.99(1)°;Z=1;V=1303.3.The final R factor is 0.0235.The rare earth ion is nine-coordinated to form a distorted monocapped square antiprism.

INHIBITIVE ACTION OF INORGANIC COMPOUND ON CORROSION OF ALUMINIUM IN HYDROCHLORIC ACID

The inhibitive action of CaSO_4 towards corrosion of aluminium in 1 mol/L HCl has been inveetigated by using fluorometrio and weight loss techniques.The results show that the inhibitor exhibits an outstanding function of inhibition,especially at higher tempe- ratures,and it was found to be predomiantly of cathodic rate control.Ths mechanism of inhibition and the effect of CdSO_4 on the corrosion kinetics are also discussed.

Study on Optimization of Ethanol Reflux Extraction of Phenolic Acids from Salvia miltiorrhiza

The extraction technology of phenolic acid compounds from Salvia miltiorrhiza by ethanol reflux was studied. In this experiment, salvianolic acid B standard was used to make the standard curve. Single factor experiment and orthogonal experiment were used to study the extraction of different ethanol concentrations, reflux times and material-to-liquid ratios. The OD value of salvianolic acid compounds was measured with a spectrophotometer. The extraction rate of phenolic acid compounds under different extraction conditions was calculated through a regression equation, so as to obtain the optimal conditions for the ethanol reflux extraction process of Salvia miltiorrhiza. The experimental data can provide a reference for the ethanol reflux extraction process of salvianolic acids in the industry. According to the experiment, the extraction rate of phenolic acids in Salvia miltiorrhiza was the highest when the ethanol concentration was 60%, the reflux time was 1.5 hours, and the ratio of material-to-liquid was 1:10.

Synthesis,Crystal Structure and Luminescence Study of a New Two-dimensional Mn(Ⅱ)Coordination Polymer

The coordination polymer [Mn2（cipt）2（aic）2]n （cipt = 2-（3-chlorophenyl）-1H- imidazo[4,5-f][1,10]phenanthroline, aic = 5-amino-isophthalic acid） has been obtained from hydrothermal reaction and characterized by elemental analysis, IR, TG and single-crystal X-ray diffraction. The title compound crystallizes in the triclinic system, space group P , with a = 11.523（3）, b = 12.162（3）, c = 18.706（5） A^°, β = 81.789（5）°, V = 2455.2（1） A^°3, C54H32Cl2Mn2N10O8, Mr = 1129.68, Z = 2, Dc = 1.528 g/cm3, μ = 0.692 mm^-1, F（000） = 1148, R = 0.064 and wR = 0.145 for 9571 observed reflections （I 〉 2σ（I））. In this polymer, the Mn（II） atoms adopt different coordination modes. The Mn（1） atom is five-coordinated, forming a distorted tetragonal pyramidal geometry. Mn（2） atom is six-coordinated to get a distorted octahedral geometry, which is different from the Mn（1） atom. Each Mn（II） atom is linked by aic ligands with neighboring Mn（II） atoms, forming an infinite one-dimensional （1D） double-chain structure. The existence of N–H…O hydrogen bonding interactions leads the 1D chains to generate a 2D structure. Luminescent properties for the ligand cipt and compound 1 have also been discussed in detail.

Influence of Mg/Al Molar Ratio on the Structures and Properties of Ru-Mg Alx Catalysts for Isomerization Linoleic Acid

With the knowledge on the significant effect of basic site and Ru on the catalytic performance of heterogeneous catalysts in the isomerization of linoleic acid to conjugated linoleic acid,a series of Ru-Mg Alx compounds with different basicity have been prepared by controlling the Mg/Al molar ratio. The results showed the catalysts with different basicity markedly influence the structure,the Ru dispersion,properties and catalytic performances for the isomerization reaction. The strong basic site is conductive to the high yield of CLA products. The strong basic site and the well-dispersion RuO2synergetic catalysis for the isomerization reaction,basic site and the well-dispersion RuO2synergetic effect are possibly via a Mg-O-Ru linkage.

Inhibition of Model Compound of Purple Acid Phosphatases on Growth of Aerobacter aerogenes Investigated by Microcalorimetry

Microcalorimetry was used to study the inhibitory or antibiotic action of six kinds of the model compounds of purple acid phosphatases on a strain of Aerobacter aerogenes . Difference in their capacities to inhibit the metabolism of this bacterium was observed. The extent and duration of the inhibitory effect on the metabolism as judged from the growth rate constant, k , and the half inhibitory concentration, IC 50 , varied with the different drugs. The rate constant k of A. aerogenes (in the log phase) in the presence of the compounds decreased with the increasing of concentrations. The experimental results reveal that the order of the antibiotic activity of the compounds is: LD 1>LD 2>LD 3>XF 1>LD 4～LD 5.

Some reactions of fluorosulfonyldifluoroacetic acid with N-heterocyclic compounds

Difluorocarbene generated from the decomposition of fluorosulfonyldifluoroacetic acid (2) reacted with various sodium salts of N-heterocyclic compounds(1) giving the corresponding difluoro- methylated products in acetonitrile at 10—40℃.Benzotriazole(1a),benzimidazole(1b) and imidazole (1c) were converted into 1-(difluoromethyl)benzotriazole(3a),1-(difluoromethyl)benzimidazole(3b) and 1-(difluoromethyl)imidazole(3c)respectively.Indole(1d)reacted with 2 to give -(fluorosulfonyldifluoro- acetate)indole(2d) rather than the expected difluoromethylated derivatives.

Divergent pathways of β,γ-unsaturated α-diazocarbonyl compounds catalyzed by dirhodium and Lewis acids catalysts separately or in combination

β,γ-Unsaturated a-diazocarbonyl compounds possess two reactive sites for electrophilic addition-one at the diazo carbon and the other at the vinylogous γ-position.Controlled by catalyst,divergent transformations are achieved starting from the same starting materials,either by Lewis acid-catalyzed addition or by dirhodium-catalyzed metal carbene reactions.In select cases two catalysts working in combination or in sequence provide a relay for cascade transformations.In this review,we summarize advances in catalyst-dependent divergent transformations of β,γ-unsaturated α-diazocarbonyl compounds and highlight the potential of this exciting research area and the many challenges that remain.

Preparation of phenyl substituted propargylic alcohol dicobalt hexacarbonyls and their reactions with active methylene compounds in the presence of acid

Eight new complexes with the formula [PhC_2C(OH)R^2R^2]Co_2(CO)_6 were prepared from phenyl substituted propargylic alcohols and dicobalt octacarbonyl.The reactions of these propargylio alcohol complexes with active methylene compounds,2,4-pentanedione or ethyl acetoacetate,in the presnce of an acid,HBF_4(40％)+P_2O_5(in excess)or BF_3·Et_2O,at room temperature in dichlorome- thane were investigated.From the 1-alkyl substituted tertiary propargylic alcohol complexes,three new conjugated ene-yne complexes produced by intramolecular dehydration reaction were isolated in high yields(82—95％).On the other hand,four new alkylated complexes were obtained with satisfactory yields(44—66％)from the secondary propargylic alcohol complexes.The influence of other acids,phosphorus pentoxide and polyphosphoric acid,on both dehydration reaction and alkylated reaction was also studied.

Synthesis, Docking and Biological Evaluation of Isoquinolonic Acid Derivatives

A series of isoquinolonic acid derivatives（4a-4o） was synthesized via one-pot synthesis for their anti-tumor activity. The structures of all the targeted compounds were confirmed by IH nuclear magnetic resonance （IH NMR） spectrometry and mass spectrometry（MS）. The anti-tumor activities of compounds 4a-4o against MG63（human osteosarcoma cells） and B16-F10（mouse melanoma cells） were examined. To evaluate the antitumor effect of the as-synthesized compounds, we compared the half maximal inhibitory concentration（1C50） of compounds 4a--4o to that of camptothecin（CPT） which appeared to be active against a broad range of human cancers. Among all the compounds, compound 41 shows the most potent biological activity against MG63 cells[IC50=（2.16i0.26） μmol/L] and B16-F10 cells[IC50=（6.95±0.24）μmol/L], thus providing useful information for the antitumor activity and potential practical use of isoquinolonic acid compounds. In addition, we screened out an efficient compound（41） that shows potential inhibit activity against Topoisomerase 1（Topo 1） by docking simulation.

Electrostatic assembly functionalization of poly(γ-glutamic acid)for biomedical antibacterial applications

Poly(γ-glutamic acid)(γ-PGA)has been found widespread applications in biomedical field because of its excellent water solubility,biocompatibility,and bioactivity.Herein,a water-insoluble γ-PGA antibacterial compound is facilely fabricated via one-pot electrostatic assembly of γ-PGA with cationic ethyl lauroyl arginate(ELA).The functionalized γ-PGA compound(γ-PGA-ELA)ethanol solution can facilely produce colorless and transparent coatings on various inorganic,metal,and polymeric substrates,especially for the lumen of slender catheters(length up to 2 m,and inner diameter down to 1 mm).The functionalized γ-PGA coating presents remarkable antibacterial efficacy in vitro and in vivo.In addition,the γ-PGA compound is used as antibacterial additives of polyolefin via melting extrusion,and the asprepared antibacterial polyolefin demonstrates advantageous antibacterial efficacy.More importantly,the functionalized γ-PGA coating exhibit good hemocompatibility,low cytotoxicity,and satisfactory histocompatibility.The as-proposed γ-PGA compound has a great potential to serve as a safe and multifunctional antibacterial candidate to combat biomedical devices-related infections.

Phosphatase activity and culture conditions of the yeast Candida mycoderma sp. and analysis of organic phosphorus hydrolysis ability

Orthophosphate is an essential but limiting macronutrient for plant growth. About 67% cropland in China lacks sufficient phosphorus, especially that with red soil. Extensive soil phosphorus reserves exist in the form of organic phosphorus, which is unavailable for root uptake unless hydrolyzed by secretory acid phosphatases. Thus, many microorganisms with the ability to produce phosphatase have been exploited. In this work, the activity of an extracellular acid phosphatase and yeast biomass from Candida mycoderma was measured under different culture conditions, such as pH, temperature, and carbon source. A maximal phosphatase activity of 8.47 × 10^5±0.11× 10^5 U/g was achieved by C. Mycoderma in 36 hr under the optimal conditions. The extracellular acid phosphatase has high activity over a wide pH tolerance range from 2.5 to S.0 （optimum pH 3.5）. The effects of different phosphorus compounds on the acid phosphatase production were also studied. The presence of phytin, lecithin or calcium phosphate reduced the phosphatase activity and biomass yield significantly. In addition, the pH of the culture medium was reduced significantly by lecithin. The efficiency of the strain in releasing orthophosphate from organic phosphorus was studied in red soil （used in planting trees） and rice soil （originating as red soil）. The available phosphorus content was increased by 230% after inoculating 20 days in rice soil and decreased by 50% after inoculating 10 days in red soil. This work indicates that the yeast strain C. mycoderma has potential application for enhancing phosphorus utilization in plants that grow in rice soil.