3-hydroxydecanoic acid (3HD) is an interesting intermediate for chemical synthesis of many valuable compounds. A novel method to produce 3HD by recombinant bacteria was constructed in Escherichia coli HB101 and Pseudo...3-hydroxydecanoic acid (3HD) is an interesting intermediate for chemical synthesis of many valuable compounds. A novel method to produce 3HD by recombinant bacteria was constructed in Escherichia coli HB101 and Pseudomonas putida GPp104, respectively. Simultaneous expression of both phaG encoding (R)-3-hydroxydecanoyl-ACP:CoA transacylase and tesB encoding thioesterase Ⅱ in E. coli HB101 increased 3HD production approximate 1.7-folds compared with the expression of phaG gene alone under identical conditions. In addition, when the tesB gene was introduced into the strain, the polyhydroxyalkanoate synthase negative strain P. putida GPpl04 produced extracellular 3HD. Thus, a novel pathway to produce 3HD by recombinant Pseudomonas was constructed. It was also found that the ratio of carbon source to nitrogen source affected the production of 3HD by recombinant P.putida harboring tesB gene. Nitrogen limitation seemed to promote the extracellular 3HD production.展开更多
Started from 5-hydroxy-2-pentanone, (R)(E)-3,7-dimethyl-2-octene-1,8-dioic acid, callosobruchusic acid, was synthesized via five steps with D-(-)-camphor sultam as the chiral auxiliary. It was of good optical purity a...Started from 5-hydroxy-2-pentanone, (R)(E)-3,7-dimethyl-2-octene-1,8-dioic acid, callosobruchusic acid, was synthesized via five steps with D-(-)-camphor sultam as the chiral auxiliary. It was of good optical purity and yield.展开更多
The title compound （R）-N′-[2-（4-methoxy-6-chloro）-pyrimidyl]-N-[3-methyl-2-（4- chlorophenyl）butyryl]-urea has been synthesized, and its crystal structure and biological behaviors were studied. Crystallographic ...The title compound （R）-N′-[2-（4-methoxy-6-chloro）-pyrimidyl]-N-[3-methyl-2-（4- chlorophenyl）butyryl]-urea has been synthesized, and its crystal structure and biological behaviors were studied. Crystallographic data： C17H18C12N4O3, Mr = 397.25, monoclinic, space group P21/c, a = 12.331（2）, b = 14.025（3）, c = 23.085（5） A, β = 99.607（4）°, Z = 8, V = 3936.2（13） A3, Dc = 1.341 g/cm^3, F（000） = 1648, R = 0.0718, wR = 0.1585 and/t（MoKα） = 0.353 mm^-1. The preliminary biological tests showed that the title compound has definite insecticidal and fungicidal activities.展开更多
A novel organic polycarboxylate N-cyclohexylaziridines-2R,3S-dicarboxylic acid was synthesized and reacted with Ni2（OH）2CO3.As a result,a coordination polymer was obtained.It crystallizes in orthorhombic,space group...A novel organic polycarboxylate N-cyclohexylaziridines-2R,3S-dicarboxylic acid was synthesized and reacted with Ni2（OH）2CO3.As a result,a coordination polymer was obtained.It crystallizes in orthorhombic,space group Pbca with a = 8.6325（10）,b = 10.5414（13）,c = 27.644（3） ,Z = 4,V = 2515.5（5） 3,C20H34N2Ni2O12,Mr = 611.91,Dc = 1.616 g/cm3,μ = 1.562 mm-1,F（000） = 1280,the final R = 0.0320 and wR = 0.0677 for 2854 independent reflections with Rint = 0.0390.Crystal structure shows that the coordination environment around the Ni（Ⅱ） ion can be described as a slightly distorted octahedron.The ligands act as a bridge through the carboxyl O atoms and join the Ni atoms into an infinite 1D chain structure.The 1D chain forms a 2D network through intermolecular O-H…O hydrogen bonds.The magnetic susceptibility measurements（2～300 K） agree with a weak antiferronmagnetic interaction between neighboring Ni ions in the chain.展开更多
Chiral amino acids and their corresponding amino alcohols bearing camphoric backbone were prepared from D-（＋）-camphoric imide and characterized by infrared, elemental analysis, ESI-MS, and NMR measurements. Among t...Chiral amino acids and their corresponding amino alcohols bearing camphoric backbone were prepared from D-（＋）-camphoric imide and characterized by infrared, elemental analysis, ESI-MS, and NMR measurements. Among them, one intermediate （1S,3R）-3-amino-2,2,3- trimethyl cyclopentane-1-carboxylic acid hydrochloride 3 was structurally elucidated by X-ray diffraction techniques. Versatile intermolecular hydrogen bonding interactions observed in its packing structure result in a two-dimensional framework.展开更多
基金Supported by the State Outstanding Young Scientist Award from National Natural Sciences Foundation of China (No. 20334020).
文摘3-hydroxydecanoic acid (3HD) is an interesting intermediate for chemical synthesis of many valuable compounds. A novel method to produce 3HD by recombinant bacteria was constructed in Escherichia coli HB101 and Pseudomonas putida GPp104, respectively. Simultaneous expression of both phaG encoding (R)-3-hydroxydecanoyl-ACP:CoA transacylase and tesB encoding thioesterase Ⅱ in E. coli HB101 increased 3HD production approximate 1.7-folds compared with the expression of phaG gene alone under identical conditions. In addition, when the tesB gene was introduced into the strain, the polyhydroxyalkanoate synthase negative strain P. putida GPpl04 produced extracellular 3HD. Thus, a novel pathway to produce 3HD by recombinant Pseudomonas was constructed. It was also found that the ratio of carbon source to nitrogen source affected the production of 3HD by recombinant P.putida harboring tesB gene. Nitrogen limitation seemed to promote the extracellular 3HD production.
基金Supported by the National Natural Science Foundation of China( No. 2 9872 0 12)
文摘Started from 5-hydroxy-2-pentanone, (R)(E)-3,7-dimethyl-2-octene-1,8-dioic acid, callosobruchusic acid, was synthesized via five steps with D-(-)-camphor sultam as the chiral auxiliary. It was of good optical purity and yield.
基金This work was sponsored by the National Key Technologies R & D Programs (No. 2004BA308A22-8)
文摘The title compound （R）-N′-[2-（4-methoxy-6-chloro）-pyrimidyl]-N-[3-methyl-2-（4- chlorophenyl）butyryl]-urea has been synthesized, and its crystal structure and biological behaviors were studied. Crystallographic data： C17H18C12N4O3, Mr = 397.25, monoclinic, space group P21/c, a = 12.331（2）, b = 14.025（3）, c = 23.085（5） A, β = 99.607（4）°, Z = 8, V = 3936.2（13） A3, Dc = 1.341 g/cm^3, F（000） = 1648, R = 0.0718, wR = 0.1585 and/t（MoKα） = 0.353 mm^-1. The preliminary biological tests showed that the title compound has definite insecticidal and fungicidal activities.
文摘A novel organic polycarboxylate N-cyclohexylaziridines-2R,3S-dicarboxylic acid was synthesized and reacted with Ni2（OH）2CO3.As a result,a coordination polymer was obtained.It crystallizes in orthorhombic,space group Pbca with a = 8.6325（10）,b = 10.5414（13）,c = 27.644（3） ,Z = 4,V = 2515.5（5） 3,C20H34N2Ni2O12,Mr = 611.91,Dc = 1.616 g/cm3,μ = 1.562 mm-1,F（000） = 1280,the final R = 0.0320 and wR = 0.0677 for 2854 independent reflections with Rint = 0.0390.Crystal structure shows that the coordination environment around the Ni（Ⅱ） ion can be described as a slightly distorted octahedron.The ligands act as a bridge through the carboxyl O atoms and join the Ni atoms into an infinite 1D chain structure.The 1D chain forms a 2D network through intermolecular O-H…O hydrogen bonds.The magnetic susceptibility measurements（2～300 K） agree with a weak antiferronmagnetic interaction between neighboring Ni ions in the chain.
基金This work was funded by the National Natural Science Foundation of China (No. 20301009)
文摘Chiral amino acids and their corresponding amino alcohols bearing camphoric backbone were prepared from D-（＋）-camphoric imide and characterized by infrared, elemental analysis, ESI-MS, and NMR measurements. Among them, one intermediate （1S,3R）-3-amino-2,2,3- trimethyl cyclopentane-1-carboxylic acid hydrochloride 3 was structurally elucidated by X-ray diffraction techniques. Versatile intermolecular hydrogen bonding interactions observed in its packing structure result in a two-dimensional framework.
