A model of focal cerebral ischemic infarction was established in dogs through middle cerebral artery occlusion of the right side.Thirty minutes after occlusion,models were injected with nerve growth factor adjacent to...A model of focal cerebral ischemic infarction was established in dogs through middle cerebral artery occlusion of the right side.Thirty minutes after occlusion,models were injected with nerve growth factor adjacent to the infarct locus.The therapeutic effect of nerve growth factor against cerebral infarction was assessed using the hemisphere anomalous volume ratio,a quantitative index of diffusion-weighted MRI.At 6 hours,24 hours,7 days and 3 months after modeling,the hemisphere anomalous volume ratio was significantly reduced after treatment with nerve growth factor. Hematoxylin-eosin staining,immunohistochemistry,electron microscopy and neurological function scores showed that infarct defects were slightly reduced and neurological function significantly improved after nerve growth factor treatment.This result was consistent with diffusion-weighted MRI measurements.Experimental findings indicate that nerve growth factor can protect against cerebral infarction,and that the hemisphere anomalous volume ratio of diffusion-weighted MRI can be used to evaluate the therapeutic effect.展开更多
目的通过对小儿特发性面神经麻痹患儿鼠神经生长因子不同治疗频度的疗效比较,探讨其在小儿特发性面神经麻痹治疗过程中的最佳治疗频度。方法选择2015年12月~2017年6月在我院神经科治疗的172例特发性面神经麻痹患儿,其中70例予常规治疗(...目的通过对小儿特发性面神经麻痹患儿鼠神经生长因子不同治疗频度的疗效比较,探讨其在小儿特发性面神经麻痹治疗过程中的最佳治疗频度。方法选择2015年12月~2017年6月在我院神经科治疗的172例特发性面神经麻痹患儿,其中70例予常规治疗(常规治疗组),54例在常规治疗基础上加用鼠神经生长因子治疗一天一次(qd组),48例在常规治疗基础上加用鼠神经生长因子治疗两天一次(qod组),观察三组的治疗疗效并加以比较。结果qd组的总有效率在疗程的7 d、14 d、28 d均明显高于常规治疗组,差异有统计学意义(7 d u=3.6037,P<0.05;14 d u=4.1778,P<0.05;28 dχ~2=8.0956,P<0.05);qod组的总有效率在疗程的28 d明显高于常规治疗组,差异有统计学意义(χ~2=5.0747,P<0.05),但在疗程的7 d、14 d时,虽高于常规治疗组,但差异无统计学意义(7 d u=0.4843,P>0.05;14 d u=1.8755,P>0.05);qd组的总有效率在7 d、14 d明显高于qod组,差异有统计学意义(7 d u=2.8328,P<0.05;14 d u=2.2417,P<0.05),但28 d时,qd组和qod组的总有效率接近,无显著的统计学差异(χ~2=0.3534,P>0.05)。结论应用鼠神经生长因子治疗小儿特发性面神经麻痹能显著提高疗效,qod组治疗成本更低,更能减轻患儿经济、精神负担。展开更多
基金supported by the Hebei Provincial Medical Science Research Key Youth Project,No.20100078
文摘A model of focal cerebral ischemic infarction was established in dogs through middle cerebral artery occlusion of the right side.Thirty minutes after occlusion,models were injected with nerve growth factor adjacent to the infarct locus.The therapeutic effect of nerve growth factor against cerebral infarction was assessed using the hemisphere anomalous volume ratio,a quantitative index of diffusion-weighted MRI.At 6 hours,24 hours,7 days and 3 months after modeling,the hemisphere anomalous volume ratio was significantly reduced after treatment with nerve growth factor. Hematoxylin-eosin staining,immunohistochemistry,electron microscopy and neurological function scores showed that infarct defects were slightly reduced and neurological function significantly improved after nerve growth factor treatment.This result was consistent with diffusion-weighted MRI measurements.Experimental findings indicate that nerve growth factor can protect against cerebral infarction,and that the hemisphere anomalous volume ratio of diffusion-weighted MRI can be used to evaluate the therapeutic effect.
文摘目的通过对小儿特发性面神经麻痹患儿鼠神经生长因子不同治疗频度的疗效比较,探讨其在小儿特发性面神经麻痹治疗过程中的最佳治疗频度。方法选择2015年12月~2017年6月在我院神经科治疗的172例特发性面神经麻痹患儿,其中70例予常规治疗(常规治疗组),54例在常规治疗基础上加用鼠神经生长因子治疗一天一次(qd组),48例在常规治疗基础上加用鼠神经生长因子治疗两天一次(qod组),观察三组的治疗疗效并加以比较。结果qd组的总有效率在疗程的7 d、14 d、28 d均明显高于常规治疗组,差异有统计学意义(7 d u=3.6037,P<0.05;14 d u=4.1778,P<0.05;28 dχ~2=8.0956,P<0.05);qod组的总有效率在疗程的28 d明显高于常规治疗组,差异有统计学意义(χ~2=5.0747,P<0.05),但在疗程的7 d、14 d时,虽高于常规治疗组,但差异无统计学意义(7 d u=0.4843,P>0.05;14 d u=1.8755,P>0.05);qd组的总有效率在7 d、14 d明显高于qod组,差异有统计学意义(7 d u=2.8328,P<0.05;14 d u=2.2417,P<0.05),但28 d时,qd组和qod组的总有效率接近,无显著的统计学差异(χ~2=0.3534,P>0.05)。结论应用鼠神经生长因子治疗小儿特发性面神经麻痹能显著提高疗效,qod组治疗成本更低,更能减轻患儿经济、精神负担。
文摘目的观察逍遥散加减联合鼠神经生长因子对原发性开角型青光眼(primary open-angle glaucoma,POAG)患者的治疗效果。方法将我院POAG患者56例(100只眼)随机分为对照组和观察组,各28例(50只眼)。观察组采用逍遥散加减联合鼠神经生长因子治疗,对照组单用注射用鼠神经生长因子治疗,两组均治疗8周。观察两组治疗前后视力、眼压、视野平均敏感度(value and mean sensitivity,MS)、视野平均缺损(mean deviation,MD)、图像视觉诱发电位(pattern visual evoked potential,P-VEP)、神经纤维层厚度变化。结果治疗后,观察组视力提升、MS值增加、MD值减小,差异均有统计学意义(P<0.05);与对照组相比,观察组视力提升、MS值增加、MD值减小,差异均有统计学意义(P<0.05);两组治疗前后P-VEP、眼压、视盘和周边视网膜神经纤维层厚度比较,差异无统计学意义(P>0.05)。观察组总有效率高于对照组,差异有统计学意义(P<0.05)。结论对于POAG患者,逍遥散加减联合注射用鼠神经生长因子能有效改善患者视力下降、视野缺损的状态,是中西医结合治疗POAG的临床有效途径,值得临床推广应用。
